18 bps of the 3'RARE were replaced by a Sal1 restriction enzyme site. A lox-P flanked neomycin cassette was inserted into the Sal1 site and was later removed via Cre-mediated recombination in the final allele. (J:38388)
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18 bps of the 3'RARE were replaced by a Sal1 restriction enzyme site. A lox-P flanked neomycin cassette was inserted into the Sal1 site and was later removed via Cre-mediated recombination in the final allele. (J:38388)