Knock-in of the Otx2 gene into the endogenous En1 locus. En1 promoter drives expression of a bicistronic Otx2 and IRES-LacZ reporter mRNA. A loxP flanked neomycin resistance casette was removed in vivo by Cre-mediated recombination. (J:57538)
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Knock-in of the Otx2 gene into the endogenous En1 locus. En1 promoter drives expression of a bicistronic Otx2 and IRES-LacZ reporter mRNA. A loxP flanked neomycin resistance casette was removed in vivo by Cre-mediated recombination. (J:57538)