Chrna7^tm1Bay

A 7 kb genomic fragment containing exons 8-10 was replaced with a neomycin selection cassette. The deleted sequences encode the second through the fourth transmembrane domains and the cytoplasmic loop. Northern blot analysis on brain samples derived from homozygous mice demonstrated that no detectable transcript was produced from this allele. Western blot analysis on brain extracts derived from homozygous mice also confirmed that no detectable protein was made from this allele. (J:44288, J:122939)