Prkdc^tm1Gcl

Half of exon 3 and part of intron 3 was replaced with a PGK-neo cassette via homologous recombination. RT-PCR showed deletion of exons 1-4 in homozygous mutant animals and Western blot failed to detect protein product. In vitro kinase assay of fibroblasts from homozygous mutant animals was negative for kinase activity. (J:53765)