A neomycin selection cassette replaced a genomic segment containing exon 5. RT-PCR analysis on RNA derived from homozygous embryos demonstrated that only an aberrant transcript which splices exon 4 to exon 6 is produced from this allele. (J:36250)
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A neomycin selection cassette replaced a genomic segment containing exon 5. RT-PCR analysis on RNA derived from homozygous embryos demonstrated that only an aberrant transcript which splices exon 4 to exon 6 is produced from this allele. (J:36250)