A PGK-neomycin based targeting vector replaced the 5' region of the gene including exons 1 and 2. Western analysis on homozygous mutant embryos demonstrated an absence of wild-type protein. (J:49339)
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A PGK-neomycin based targeting vector replaced the 5' region of the gene including exons 1 and 2. Western analysis on homozygous mutant embryos demonstrated an absence of wild-type protein. (J:49339)