Pomc^tm1Low

A PGK-neomycin based targeting vector was used to introduce a premature start codon into exon 3, resulting in the truncation of the translated protein. In situ hybridization on homozygous mutant animals demonstrates that the truncation event had no effect on the distribution or level of transcript. Immunoreactivity demonstrated that Homozygous mutant mice had no detectable beta endorphin activity in the hypothalamus or pituitary (J:78759)