Ncam1^tm1Cgn

A neomycin resistance cassette replaced most of exons 3 and 4 and the intervening intron. Ribonuclease protection assays did not detect wild-type or read-through transcripts or alternative or cryptic splicing in brain tissue from homozygous mutant mice. Western blot analysis of brain tissue using a monoclonal antibody or a polyclonal rabbit serum did not detect protein in homozygous mutant mice. Similarly, immunostaining did not detect protein in mutant olfactory bulb. (J:16590)