Mreg^dsu

Genomic PCR and DNA sequencing analysis showed the mutation is a deletion that spans the region from 11.1-kb upstream of the initiator methionine to intron 2. Northern and RT-PCR analysis demonstrated that no wild-type transcript could be detected in the mutant animals. Western blot analysis of homozygous mutant melanocytes showed that it also is not expressed at the protein level. (J:94728)