Dao is located on chromosome 5 of mice. CRISPR/Cas technology was used to design sgRNA; Dao knockout mice were obtained by applying high-throughput electroporation of fertilized eggs. After sexual maturity, sperm were collected for cryopreservation.
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Dao is located on chromosome 5 of mice. CRISPR/Cas technology was used to design sgRNA; Dao knockout mice were obtained by applying high-throughput electroporation of fertilized eggs. After sexual maturity, sperm were collected for cryopreservation.