Enables pseudouridine synthase activity. Acts upstream of or within tRNA pseudouridine synthesis. Predicted to be active in cytoplasm and nucleus. Is expressed in several structures, including alimentary system; heart ventricle; pharyngo-tympanic tube; pituitary gland; and vertebral axis musculature. Orthologous to human PUS3 (pseudouridine synthase 3). [provided by Alliance of Genome Resources, Apr 2022]