Enables heparan sulfate 6-O-sulfotransferase activity. Acts upstream of or within heparan sulfate proteoglycan biosynthetic process, enzymatic modification. Predicted to be located in membrane. Predicted to be integral component of membrane. Is expressed in several structures, including central nervous system; dorsal aorta; genitourinary system; limb primordium; and sensory organ. Orthologous to human HS6ST3 (heparan sulfate 6-O-sulfotransferase 3). [provided by Alliance of Genome Resources, Apr 2022]