Enables [heparan sulfate]-glucosamine 3-sulfotransferase 1 activity. Acts upstream of or within blastocyst hatching. Predicted to be located in Golgi apparatus and membrane. Predicted to be integral component of membrane. Is expressed in alimentary system; early conceptus; integumental system; and nervous system. Orthologous to human HS3ST6 (heparan sulfate-glucosamine 3-sulfotransferase 6). [provided by Alliance of Genome Resources, Apr 2022]