Predicted to enable DNA binding activity and endonuclease activity. Predicted to be involved in DNA metabolic process and mitotic intra-S DNA damage checkpoint signaling. Predicted to act upstream of or within response to intra-S DNA damage checkpoint signaling. Predicted to be located in heterochromatin. Predicted to be part of Holliday junction resolvase complex. Orthologous to human EME1 (essential meiotic structure-specific endonuclease 1). [provided by Alliance of Genome Resources, Apr 2022]