Contributes to ATP hydrolysis activity. Involved in ATP metabolic process. Located in mitochondrial inner membrane. Part of mitochondrial proton-transporting ATP synthase complex, catalytic sector F(1). Human ortholog(s) of this gene implicated in mitochondrial complex V (ATP synthase) deficiency nuclear type 3. Orthologous to several human genes including ATP5F1E (ATP synthase F1 subunit epsilon). [provided by Alliance of Genome Resources, Apr 2022]