Enables heparan sulfate 2-O-sulfotransferase activity. Acts upstream of or within several processes, including heparan sulfate proteoglycan biosynthetic process, polysaccharide chain biosynthetic process; heparin metabolic process; and ureteric bud formation. Predicted to be located in Golgi apparatus and membrane. Predicted to be integral component of membrane. Is expressed in brain and future brain. Orthologous to human HS2ST1 (heparan sulfate 2-O-sulfotransferase 1). [provided by Alliance of Genome Resources, Apr 2022]