Predicted to enable myosin light chain binding activity and myosin light chain kinase activity. Involved in skeletal muscle satellite cell differentiation. Acts upstream of or within neuromuscular synaptic transmission. Predicted to be located in several cellular components, including dendritic spine; postsynaptic density; and synaptic vesicle. Predicted to be active in cytoplasm. Human ortholog(s) of this gene implicated in hypertrophic cardiomyopathy 1. Orthologous to human MYLK2 (myosin light chain kinase 2). [provided by Alliance of Genome Resources, Apr 2022]