Enables phosphopyruvate hydratase activity. Acts upstream of or within cilium organization; flagellated sperm motility; and glycolytic process. Located in sperm principal piece. Is expressed in choroid invagination; embryo; metencephalon part of 4th ventricle choroid plexus; and olfactory epithelium. Orthologous to human ENO4 (enolase 4). [provided by Alliance of Genome Resources, Apr 2022]