Predicted to enable S-adenosylmethionine-dependent tRNA (m5U54) methyltransferase activity. Predicted to be involved in RNA processing. Predicted to act upstream of or within methylation and tRNA processing. Located in mitochondrion. Is expressed in olfactory cortex ventricular layer and telencephalon ventricular layer. Orthologous to human TRMT2B (tRNA methyltransferase 2 homolog B). [provided by Alliance of Genome Resources, Apr 2022]