Enables tubulin-tyrosine ligase activity. Predicted to be involved in C-terminal protein-tyrosinylation; positive regulation of mitotic cell cycle; and regulation of metaphase plate congression. Predicted to act upstream of or within microtubule cytoskeleton organization and regulation of axon extension. Predicted to be located in spindle microtubule. Orthologous to human TTL (tubulin tyrosine ligase). [provided by Alliance of Genome Resources, Apr 2022]