Enables acetyl-CoA C-acetyltransferase activity. Predicted to be involved in carboxylic acid catabolic process; ketone body catabolic process; and purine nucleoside bisphosphate metabolic process. Predicted to act upstream of or within fatty acid metabolic process. Located in mitochondrial inner membrane. Is expressed in brain; embryo; heart; liver; and metanephros. Human ortholog(s) of this gene implicated in beta-ketothiolase deficiency and carbohydrate metabolic disorder. Orthologous to human ACAT1 (acetyl-CoA acetyltransferase 1). [provided by Alliance of Genome Resources, Apr 2022]