Enables tRNA 2'-phosphotransferase activity. Acts upstream of or within regulation of protein kinase activity and tRNA splicing, via endonucleolytic cleavage and ligation. Is expressed in several structures, including gonad; intestine; liver; lung; and spleen. Orthologous to human TRPT1 (tRNA phosphotransferase 1). [provided by Alliance of Genome Resources, Apr 2022]