LOCUS       NM_199104               5418 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Rattus norvegicus zinc finger FYVE-type containing 27 (Zfyve27),
            mRNA.
ACCESSION   NM_199104 XM_219886
VERSION     NM_199104.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 5418)
  AUTHORS   Brachet,A., Lario,A., Fernandez-Rodrigo,A., Heisler,F.F.,
            Gutierrez,Y., Lobo,C., Kneussel,M. and Esteban,J.A.
  TITLE     A kinesin 1-protrudin complex mediates AMPA receptor synaptic
            removal during long-term depression
  JOURNAL   Cell Rep 36 (5), 109499 (2021)
   PUBMED   34348158
  REMARK    GeneRIF: A kinesin 1-protrudin complex mediates AMPA receptor
            synaptic removal during long-term depression.
REFERENCE   2  (bases 1 to 5418)
  AUTHORS   Hashimoto,Y., Shirane,M., Matsuzaki,F., Saita,S., Ohnishi,T. and
            Nakayama,K.I.
  TITLE     Protrudin regulates endoplasmic reticulum morphology and function
            associated with the pathogenesis of hereditary spastic paraplegia
  JOURNAL   J Biol Chem 289 (19), 12946-12961 (2014)
   PUBMED   24668814
REFERENCE   3  (bases 1 to 5418)
  AUTHORS   Ohnishi,T., Shirane,M., Hashimoto,Y., Saita,S. and Nakayama,K.I.
  TITLE     Identification and characterization of a neuron-specific isoform of
            protrudin
  JOURNAL   Genes Cells 19 (2), 97-111 (2014)
   PUBMED   24251978
  REMARK    Erratum:[Genes Cells. 2020 Sep;25(9):646. doi: 10.1111/gtc.12799..
            PMID: 32926506]
REFERENCE   4  (bases 1 to 5418)
  AUTHORS   Chang,J., Lee,S. and Blackstone,C.
  TITLE     Protrudin binds atlastins and endoplasmic reticulum-shaping
            proteins and regulates network formation
  JOURNAL   Proc Natl Acad Sci U S A 110 (37), 14954-14959 (2013)
   PUBMED   23969831
REFERENCE   5  (bases 1 to 5418)
  AUTHORS   Matsuzaki,F., Shirane,M., Matsumoto,M. and Nakayama,K.I.
  TITLE     Protrudin serves as an adaptor molecule that connects KIF5 and its
            cargoes in vesicular transport during process formation
  JOURNAL   Mol Biol Cell 22 (23), 4602-4620 (2011)
   PUBMED   21976701
REFERENCE   6  (bases 1 to 5418)
  AUTHORS   Saita,S., Shirane,M., Natume,T., Iemura,S.I. and Nakayama,K.I.
  TITLE     Promotion of neurite extension by protrudin requires its
            interaction with vesicle-associated membrane protein-associated
            protein
  JOURNAL   J Biol Chem 284 (20), 13766-13777 (2009)
   PUBMED   19289470
  REMARK    GeneRIF: VAP-A is an important regulator both of the subcellular
            localization of protrudin and of its ability to stimulate neurite
            outgrowth.
REFERENCE   7  (bases 1 to 5418)
  AUTHORS   Shirane,M. and Nakayama,K.I.
  TITLE     Protrudin induces neurite formation by directional membrane
            trafficking
  JOURNAL   Science 314 (5800), 818-821 (2006)
   PUBMED   17082457
  REMARK    GeneRIF: protrudin regulates Rab11-dependent membrane recycling to
            promote the directional membrane trafficking required for neurite
            formation [protrudin]
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000001.1.
            
            On Aug 31, 2012 this sequence version replaced NM_199104.1.
            
            Sequence Note: This RefSeq record was created from genomic sequence
            data to make the sequence consistent with the reference genome
            assembly. The genomic coordinates used for the transcript record
            were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC061741.1, SRR26360194.2083414.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760389
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-185               JAXUCZ010000001.1  250929131-250929315
            186-383             JAXUCZ010000001.1  250930031-250930228
            384-454             JAXUCZ010000001.1  250935457-250935527
            455-641             JAXUCZ010000001.1  250937398-250937584
            642-737             JAXUCZ010000001.1  250939565-250939660
            738-850             JAXUCZ010000001.1  250940939-250941051
            851-990             JAXUCZ010000001.1  250941629-250941768
            991-1062            JAXUCZ010000001.1  250942306-250942377
            1063-1207           JAXUCZ010000001.1  250943964-250944108
            1208-1254           JAXUCZ010000001.1  250947098-250947144
            1255-1336           JAXUCZ010000001.1  250947411-250947492
            1337-5418           JAXUCZ010000001.1  250948400-250952481
FEATURES             Location/Qualifiers
     source          1..5418
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q54"
     gene            1..5418
                     /gene="Zfyve27"
                     /note="zinc finger FYVE-type containing 27"
                     /db_xref="GeneID:309376"
                     /db_xref="RGD:735177"
     exon            1..185
                     /gene="Zfyve27"
                     /inference="alignment:Splign:2.1.0"
     exon            186..383
                     /gene="Zfyve27"
                     /inference="alignment:Splign:2.1.0"
     CDS             187..1401
                     /gene="Zfyve27"
                     /note="zinc finger FYVE domain-containing protein 27; zinc
                     finger, FYVE domain containing 27"
                     /codon_start=1
                     /product="protrudin"
                     /protein_id="NP_954535.2"
                     /db_xref="GeneID:309376"
                     /db_xref="RGD:735177"
                     /translation="MQSSDRDLSGPEASPSVMPEVLSECSPAPTKSTAFDLFNLVLSY
                     KRLEIYLEPLKDAGDGVRYLLRWQMPLCSLLTCLGLNILFLTLNEGAWYSVGALIISV
                     PALLGYLQEVCRAQLPESELMRRKYHSVRQEDLQRVRLSRPEAVAEVKSFLIRLEAFL
                     ARLCYTCESAYRVLHWENPVVSSQFYGALLGMVCMLYLLPLCWVLALLNSTLFLGNGE
                     FFRVVSEYRACLQRRMSPKQEECVCEGSALQDAGGRAVVLDSTPAPTPTEDLTPGSVE
                     EAEEAEPDEEFKDAIEEDDEGTPCPAEDELTMQDNGFLSKNEVLRSKVSKLTERLRKR
                     YPTNNFGNCAGCAATFSVLKKRRSCSNCGNSFCSRCCSFKVPKSSMGATAPEAQRETV
                     FVCASCNQTLSK"
     exon            384..454
                     /gene="Zfyve27"
                     /inference="alignment:Splign:2.1.0"
     exon            455..641
                     /gene="Zfyve27"
                     /inference="alignment:Splign:2.1.0"
     exon            642..737
                     /gene="Zfyve27"
                     /inference="alignment:Splign:2.1.0"
     exon            738..850
                     /gene="Zfyve27"
                     /inference="alignment:Splign:2.1.0"
     exon            851..990
                     /gene="Zfyve27"
                     /inference="alignment:Splign:2.1.0"
     exon            991..1062
                     /gene="Zfyve27"
                     /inference="alignment:Splign:2.1.0"
     exon            1063..1207
                     /gene="Zfyve27"
                     /inference="alignment:Splign:2.1.0"
     exon            1208..1254
                     /gene="Zfyve27"
                     /inference="alignment:Splign:2.1.0"
     exon            1255..1336
                     /gene="Zfyve27"
                     /inference="alignment:Splign:2.1.0"
     exon            1337..5418
                     /gene="Zfyve27"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gtcgcgtccc ggaagcgacg gcggtggtgg ggcagactgt tctctggtgg acttgcgatt
       61 tcggagcgtc tgtgggaggt ggcgcggcgt ggggtggggc gggcctgagg ggccgctccc
      121 gggcttggtg gcccttctgt ccccttgggc gcccctgccc ctcccattcc tgggacagtg
      181 tccggaatgc agtcttcgga tcgggacctg agtggaccag aggcgagccc cagcgtgatg
      241 cctgaggttc tctctgagtg ttcacctgcc cctaccaagt caacagcgtt tgatcttttc
      301 aacctggttc tgtcctacaa gaggctggag atctacctgg aacccctgaa ggatgcaggt
      361 gacggtgttc gatacttgct aaggtggcag atgcctttgt gttccttgct gacttgcctg
      421 ggcctcaaca tcttgttcct cactctgaat gagggtgcat ggtactccgt gggtgccttg
      481 ataatttcgg tgcctgccct actgggctac cttcaggagg tgtgccgggc acagctacca
      541 gagtctgagc tgatgaggcg gaagtaccac agcgtgcggc aggaagacct gcagagagtt
      601 cgcctttccc gccctgaggc tgtagctgag gtgaaaagct tcttgatccg actggaagcc
      661 ttcttggccc gcctgtgcta tacctgcgag tcagcttacc gtgtacttca ctgggagaac
      721 cctgtggtgt cctcacagtt ctatggtgct cttctgggca tggtttgcat gctctacctg
      781 ctgccgctct gctgggtcct cgccctttta aacagcacac tctttctggg aaatggggaa
      841 ttcttccgag tggtgtctga gtacagggct tgtctgcagc ggcggatgag ccccaagcag
      901 gaagagtgcg tctgtgaggg ctcggcactg caggatgccg gggggagggc tgttgtactg
      961 gacagcaccc ctgcccctac acccacagag gacctcacgc caggcagtgt ggaggaagct
     1021 gaggaggctg agccagatga ggagttcaaa gatgcaatag aggaggacga tgagggaacc
     1081 ccatgcccag cagaggatga gctgaccatg caggacaatg gcttcctcag caagaatgag
     1141 gtactgcgca gcaaggtgtc gaagcttaca gagcggctcc gcaagcgcta cccgaccaac
     1201 aacttcggga actgtgcagg ctgtgctgcc accttctccg tgctgaagaa gaggcgaagc
     1261 tgcagcaact gtgggaacag cttctgctct cggtgctgct ccttcaaggt gcccaagtcc
     1321 tccatggggg ccacagctcc tgaagcccag agagagactg tgtttgtgtg tgcctcctgt
     1381 aatcagacct tgagcaaatg agaagagaag ccaggctcca gctgccctgg ggccagcagt
     1441 agacccaact gccccaactt tggtcctctg aggcctgtgc tggacagatg tagcctgaca
     1501 gctaggccct tcctgctctt tccctgctgc ctccagctgg gctggtgaca ctctaaggcc
     1561 cagggggaga ggagcctctg gggtcctggc tgttggtcct gttctaccct gtaccctctt
     1621 aactcaaggc cagccaggga gtgtccacca gagggcagaa gtgagcacag cctgctctca
     1681 tctgggctct ggtggtcaaa gccagactgg ctaggccttg ggatccaagg agaccggagg
     1741 ttggaaaaag aggttcttcc ccattgggta ctgggcagtc tcagccccag agcctgcaag
     1801 gggacttaga gcagcatggc ctgactctgc cctttgaata gccccaggac atgttggttt
     1861 ttagagacag gattggagtt accaggtgcc ccgaggagag ggaatgtgag ggtgctggtc
     1921 aagtccttca gtgtcacgtg gatggatggc agtgtggggg agctctacct acagaatctt
     1981 agctcccatg attctctggt gccctcagcc ctgctcgggt ggctacaggg ccctcacatg
     2041 attcctactt cctgttctga gtctggggca ccccaccttt ctccactgcc acatctctct
     2101 agcccttatc tcatggaaca tgaggccagg cgagtggagc agagagtctg gaagcctttg
     2161 aggttggggg aagtatggat cgcctggatt tcagggctgt tcctagagag gagtttacat
     2221 cgtaggatag ggcacatggc cagttttatg ttccctcttc tctgccaagg gggcgctgca
     2281 cctcttctta agccatgagc tggctcaggc ccctccccca gagctttttc agagtattta
     2341 tttatttatt tcatggttcc tgggagcatg cagcacaggg tgggatgaaa acgatgaggg
     2401 gcttctgcct gcgacccttc tgtagccatg gaccgccctt ggggcagatc ttcaggaact
     2461 tgcatcagta gcagcggagc tgtttctacc caagcatgga cttggcgcgc acacttgagc
     2521 cgcatgagtg actctctgct gatgggcccc acacagaaca gtgagggttt ggcagtgggg
     2581 accaagcagg tgaggtgtac ccctttgtcc tggtgagaga aggtcacctc tgtgtattta
     2641 tactgtagag ctgtagaaag gaactctgtg gagaattgtc aataaaaggc cccacgctta
     2701 tggttttgtc aggtctagtg ctgttcggtc tttcacattg cccaggctgg gctgtaggtg
     2761 gatcttgcac accttctgtg gcagtggtga gccagccagg cccagtggaa aactgcctta
     2821 gcagaaggag gggagggatc tgtcttgtga tcagacccag ggccttggat tgaggttgga
     2881 accagaactt aactataagt aatgcaggct tcagaatgga aaggttttat cgaatcctct
     2941 gggaaagaca ggaaggccag cactgcagga gccggagaac tagtaaagag gttcgtggag
     3001 ggcgaggtga tccagattat gttggtgctg ggcactgcac acgttatgca ggtagcagca
     3061 gcaagcccat gaggtaggct ctgatgtcca catggtaagg cagcagctct gcgagccagc
     3121 tctgagctac cacccaccat gcacctgtgt gccatcatcc tacagccttc acaaggaatc
     3181 tctgttctgt gtccctatag cagaagtctt ttcctcctct tcctcctctc cctctttctt
     3241 tctcctctcc tcttcttcct cctcctccca caaagaatga ttttaagtct tttttttaaa
     3301 agaaggtatc agggagagag ttcagagtat agtttgaaat agttgatgtc atcttactga
     3361 aaggtgactg tcactgctca gaaaggctga ctgtagtatg tagtcacaga tgtgagaaaa
     3421 ggggcttcct tttttcgttt cctggaggtc cctgtgtgaa caggtgggag gctgtcttca
     3481 aattcaggtt tcttctttga gaacagagcc accagagttc agcagggttg gtggtggtag
     3541 tggtgtcacc acggagcctg tggtgcagag ttctctaggt gggatgacag acagcttgca
     3601 cgctgtggaa ctggatgtct gtgtcaaaag acacctgcta gtttgatggg cacccaggaa
     3661 cagggtcaga gagccaggtt cggagggtcc tggcacgtgg cagggcagct ctgggactct
     3721 ggcatccagc ctgacttggg ctcaggtctg ttgagggttt tgtggtccag tgtgtcgatt
     3781 ccttcagaaa gtcttggcag acctggaaca ggcaatggtt tggggaggtg ttgtctgtag
     3841 cccttgggac tgaagccaaa tggatctcct ttagaggtct gtgtttgtct tcccacagct
     3901 ggccctacgc acggctcatt ttgccaggga acgtgtccca ccgtgccaca ggttccccta
     3961 attcgtggat atcttttttt tttttttttt gggttctttt tttcggagct ggggaccgaa
     4021 cccagggcct tgcgcttcct aggtaagcgt tctaccactg agctaaatcc ccagccccta
     4081 ttcgaggata tcttaaatgg gcagatggag gtgcaggggg gtcaagtgag tgggaattgt
     4141 gttgcaggtg ttcatagcag ccatggccta agaatctctg ggtaaaggtc acatattctg
     4201 gtccccactc taaggagcta gccacccagc taacaagaac tgtctgccct cccacccccg
     4261 ccccagcacc atcccccttc agtctctacc agaacagaga tggccttgtg gcaggagccc
     4321 ccacttctcc cagtgacccc gcctcctgcc agcaacaccc cagcagcaca ttccattctc
     4381 tggccctgct ggtggctgcc gattcaggtt ccttcggcca taagccttct ctggggctcc
     4441 agcagaggct gtttgggctg ggacactggg ctccagccac acatattcca gcaacaagca
     4501 ccccccgcat tagatttccc aaggagtgaa cttcaattca gccaggatta cagatggcct
     4561 tacagttgtg ggatatgggc ttgctttgtg tagacctgag gctggacact agtaggggtg
     4621 aagaggagtg ggtctgcttc tgctggggcc ttgtagcatt actggagcag cctcacgatt
     4681 gcctgtgccc ttgtcccacc tcacttctgt agatgggagt aatagtctgc agactttcaa
     4741 gctgtgactc catatgctgt cctgtactgc atgcgaggat taggggaagt ttggtgacag
     4801 caaaaggctc tcaaactctg agcaactaaa aaccaactca aagccaaact cgaaaaccca
     4861 tcggaggtgt ttgtggagca ctgtcctgag cactgcaccc tgtagattcc tcatgccaca
     4921 gaggcagtga aaaccccttg gatcgtacaa cgtgaggcca ctgcatgttg tgaactacag
     4981 tgtttttact gtacgttcag ttcctgcttt tacaggaaca tggtggttta cagaaaataa
     5041 ggacttacta atggccctac ttgcatgtaa atatctaagg agagtatgtc attggattat
     5101 tatgttggaa tacttgattt taaaatcggt tactgtttgt ggacgggtga gatggctcac
     5161 taggtaagcc ctgcccacaa aggctggacc cctgactttg atccctggga ccttgttaaa
     5221 aagccagccg tgttgcatct gtaatctcag cattcttgta gagaaatgca aggagacaag
     5281 aggatggccc tggagagtgc agcagtctgg cataaactag agtctgcctg aaaaagactg
     5341 aaggagagaa ccaagccctc tgcatctaca catgcactgt agcacaggca ataataaaaa
     5401 ttaaaagaga aatgaaaa
//
