LOCUS       NM_001401428            2396 bp    mRNA    linear   ROD 13-FEB-2025
DEFINITION  Rattus norvegicus zinc finger CCHC-type containing 4 (Zcchc4),
            mRNA.
ACCESSION   NM_001401428 XM_017599294
VERSION     NM_001401428.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Pinto,R., Vagbo,C.B., Jakobsson,M.E., Kim,Y., Baltissen,M.P.,
            O'Donohue,M.F., Guzman,U.H., Malecki,J.M., Wu,J., Kirpekar,F.,
            Olsen,J.V., Gleizes,P.E., Vermeulen,M., Leidel,S.A., Slupphaug,G.
            and Falnes,P.O.
  TITLE     The human methyltransferase ZCCHC4 catalyses N6-methyladenosine
            modification of 28S ribosomal RNA
  JOURNAL   Nucleic Acids Res 48 (2), 830-846 (2020)
   PUBMED   31799605
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Ren,W., Lu,J., Huang,M., Gao,L., Li,D., Wang,G.G. and Song,J.
  TITLE     Structure and regulation of ZCCHC4 in m6A-methylation of 28S rRNA
  JOURNAL   Nat Commun 10 (1), 5042 (2019)
   PUBMED   31695039
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2396)
  AUTHORS   van Tran,N., Ernst,F.G.M., Hawley,B.R., Zorbas,C., Ulryck,N.,
            Hackert,P., Bohnsack,K.E., Bohnsack,M.T., Jaffrey,S.R., Graille,M.
            and Lafontaine,D.L.J.
  TITLE     The human 18S rRNA m6A methyltransferase METTL5 is stabilized by
            TRMT112
  JOURNAL   Nucleic Acids Res 47 (15), 7719-7733 (2019)
   PUBMED   31328227
REFERENCE   4  (bases 1 to 2396)
  AUTHORS   Ma,H., Wang,X., Cai,J., Dai,Q., Natchiar,S.K., Lv,R., Chen,K.,
            Lu,Z., Chen,H., Shi,Y.G., Lan,F., Fan,J., Klaholz,B.P., Pan,T.,
            Shi,Y. and He,C.
  TITLE     N6-Methyladenosine methyltransferase ZCCHC4 mediates ribosomal RNA
            methylation
  JOURNAL   Nat Chem Biol 15 (1), 88-94 (2019)
   PUBMED   30531910
  REMARK    Erratum:[Nat Chem Biol. 2019 May;15(5):549. doi:
            10.1038/s41589-019-0233-6.. PMID: 30737498]
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000014.1.
            
            On Feb 11, 2022 this sequence version replaced XM_017599294.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26360181.1141182.1,
                                           SRR26360176.524665.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-151               JAXUCZ010000014.1  62419394-62419544   c
            152-270             JAXUCZ010000014.1  62418408-62418526   c
            271-353             JAXUCZ010000014.1  62417121-62417203   c
            354-629             JAXUCZ010000014.1  62405777-62406052   c
            630-707             JAXUCZ010000014.1  62405247-62405324   c
            708-780             JAXUCZ010000014.1  62397749-62397821   c
            781-931             JAXUCZ010000014.1  62395124-62395274   c
            932-1032            JAXUCZ010000014.1  62393793-62393893   c
            1033-1154           JAXUCZ010000014.1  62386382-62386503   c
            1155-1230           JAXUCZ010000014.1  62386068-62386143   c
            1231-1282           JAXUCZ010000014.1  62383837-62383888   c
            1283-1427           JAXUCZ010000014.1  62383285-62383429   c
            1428-2396           JAXUCZ010000014.1  62378269-62379237   c
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="14"
                     /map="14q11"
     gene            1..2396
                     /gene="Zcchc4"
                     /note="zinc finger CCHC-type containing 4"
                     /db_xref="GeneID:360946"
                     /db_xref="RGD:1311414"
     exon            1..151
                     /gene="Zcchc4"
                     /inference="alignment:Splign:2.1.0"
     CDS             28..1497
                     /gene="Zcchc4"
                     /note="zinc finger CCHC domain-containing protein 4; zinc
                     finger, CCHC domain containing 4; rRNA
                     N6-adenosine-methyltransferase ZCCHC4"
                     /codon_start=1
                     /product="rRNA N(6)-adenosine-methyltransferase ZCCHC4"
                     /protein_id="NP_001388357.1"
                     /db_xref="GeneID:360946"
                     /db_xref="RGD:1311414"
                     /translation="MAAPMDCLESLEGEGSVGRRASGVEVALPSNPGAPAPQCPHGPT
                     LLFVKVSQGKEEPRKFYACSACRDRKDCNFFQWEDEKLSEARLAAREIHNQRCQPPLS
                     RAQCIERYLSFIQLPLTQRKFCRSCQQLLLPADWREHGEHQLSADISTTQIARPSQLL
                     YPLENKKTHAQYLFADRSCQFLAGLLATLGFRRVLCVGAPRLHEQIRLTAPGEKPDMR
                     SLLLDIDFRYSQFYLEESFCRYNMFNHHFFDGKAALEVCKAFLQEEEGKGVIMVTDPP
                     FGGLVEPLAVTFKKLIAMWKEGQSQDDSHKELPIFWIFPYFFESRICQFFPSFCMLDY
                     QVDYDNHALYKHGKTGRKQSPVRIFTNIPPNKIILPSEEGYRFCSVCQRYVSRENQHC
                     VHCNSCTSKDGRKWSHCFFCKKCVKPSWIHCNTCNRCALPDHSCLGPKDGCFICGSLD
                     HKRSNCPSIGASQRANNAVRKQKQRKSNKIRREALKDNP"
     exon            152..270
                     /gene="Zcchc4"
                     /inference="alignment:Splign:2.1.0"
     exon            271..353
                     /gene="Zcchc4"
                     /inference="alignment:Splign:2.1.0"
     exon            354..629
                     /gene="Zcchc4"
                     /inference="alignment:Splign:2.1.0"
     exon            630..707
                     /gene="Zcchc4"
                     /inference="alignment:Splign:2.1.0"
     exon            708..780
                     /gene="Zcchc4"
                     /inference="alignment:Splign:2.1.0"
     exon            781..931
                     /gene="Zcchc4"
                     /inference="alignment:Splign:2.1.0"
     exon            932..1032
                     /gene="Zcchc4"
                     /inference="alignment:Splign:2.1.0"
     exon            1033..1154
                     /gene="Zcchc4"
                     /inference="alignment:Splign:2.1.0"
     exon            1155..1230
                     /gene="Zcchc4"
                     /inference="alignment:Splign:2.1.0"
     exon            1231..1282
                     /gene="Zcchc4"
                     /inference="alignment:Splign:2.1.0"
     exon            1283..1427
                     /gene="Zcchc4"
                     /inference="alignment:Splign:2.1.0"
     exon            1428..2396
                     /gene="Zcchc4"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 agaggctttc gggacctcgg cggaaagatg gcggcgccca tggactgtct tgagtccctg
       61 gagggtgaag gcagcgtggg gcgtagggcg tccggggtgg aggtggcgct tccctcgaac
      121 cccggggctc ccgccccgca gtgcccacac gggcccactc ttctctttgt aaaagtgagc
      181 caagggaagg aagaaccacg caaattttat gcctgctcag cctgtagaga tagaaaagac
      241 tgtaattttt ttcaatggga agatgaaaag ttgtcagaag ctagacttgc tgcccgggaa
      301 attcataatc aaagatgcca gcctcctcta tcccgagcac aatgcataga aaggtacctg
      361 agtttcattc agttgccatt gactcaaaga aagttctgtc gaagttgtca gcaattgctg
      421 ttacctgcag attggaggga gcatggcgag caccagctat cagctgacat ttccaccacc
      481 cagattgcaa ggcccagtca gctgctctac ccgctggaga acaagaagac acacgcccag
      541 tacctgtttg ccgataggag ctgccagttc ctggcaggcc tccttgccac cctcggattc
      601 agaagagtgc tgtgtgttgg agcaccaagg ctacacgagc agatcaggct aacagcacca
      661 ggtgagaagc ccgacatgag gagcctttta ttggatattg actttcgata ttcacagttt
      721 tacctggaag agagcttttg ccgctataac atgttcaacc atcatttctt tgatggaaag
      781 gctgcccttg aagtgtgcaa ggcattctta caggaagagg aaggcaaggg ggtgattatg
      841 gtgacagacc ctccctttgg cggcttggtt gagccactgg ctgttacatt caagaagtta
      901 attgctatgt ggaaagaagg tcaaagccaa gatgacagtc acaaagaact gcccattttc
      961 tggattttcc catatttttt tgagtcacgg atttgtcagt tttttccgag cttctgcatg
     1021 ctggattacc aggtagatta tgataatcat gcgctttaca aacatggaaa gacaggtcga
     1081 aaacagtctc ctgtccggat tttcaccaac attccaccca acaaaataat ccttccatca
     1141 gaggaagggt acaggttttg ctcagtctgt cagagatacg tctctcgcga gaaccagcac
     1201 tgtgtgcatt gtaattcctg cacatccaag gatggcagga aatggagcca ttgctttttc
     1261 tgcaaaaagt gtgtgaagcc ttcctggatc cattgtaaca cctgcaatcg ctgtgcactc
     1321 ccagaccatt cctgtttggg gccgaaagat ggctgcttta tttgtggttc gttggatcat
     1381 aaacgcagca attgtcccag cattggcgcc tcgcagagag ctaacaacgc tgtcagaaag
     1441 cagaagcaaa gaaaaagtaa taagataaga agggaggcac taaaggacaa tccatgaatc
     1501 acacatctgc tccgagaaga aggaagagga ggaaaagggc ccatcaatat cctcgctcct
     1561 aaatgtccag tgacttgaga ataagcaaga tttacagtcc aacctttgat gccattttct
     1621 ccgagtgcca cactggactt aaatccagct gctttcaaag gtgtgcccct tcgtaaggct
     1681 ggtaacaggc aggtggcatt tgctggctga gtcccttcag ctgcctctgc agacccggga
     1741 gccttctgta cgcagtaggt tgcctggctc tctctctaca tgtctttcca ctctttaact
     1801 cctgacaagt ctttattttg gcctcttttg tctgaaaaat gggcatacag catatgttta
     1861 cttacaatac cagatagaaa tgcaaatgaa aatctatact tttccttatg tcagtcaagc
     1921 ttttgtttta atttaggtat gaaacttctg ttagtgacca cgcagtacat taaagcacac
     1981 agcccatggg ccacatgtgc cccaggatag ctatgaacgt agcctaacac cgaatcataa
     2041 gcacacttaa aactgagact ttgtgtgact ttggtcataa cttgttctca cagactttga
     2101 taggaacttt gtagatgatg tcgcgttgca atgtcaaaag ttggacacat ggggttgggg
     2161 atttagattt agctcagtgg tagagcgctt gcctagcaac tgcaaggccc tgggttctgt
     2221 ccccagctcc gaaaaaaaaa aaaaaaagtt ggacacaccc agactctttc aacaaaaagt
     2281 gtgatacata attatttata gattgcatct ggagataatg ggggaagttg tcattgaagc
     2341 actgtaaaga taaaaagatt gtaattgttg ttttattatt aaatctttat ttttca
//
