LOCUS       NM_175579               4093 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus WNK lysine deficient protein kinase 4 (Wnk4),
            mRNA.
ACCESSION   NM_175579
VERSION     NM_175579.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 4093)
  AUTHORS   Bhuiyan,M.I.H., Song,S., Yuan,H., Begum,G., Kofler,J., Kahle,K.T.,
            Yang,S.S., Lin,S.H., Alper,S.L., Subramanya,A.R. and Sun,D.
  TITLE     WNK-Cab39-NKCC1 signaling increases the susceptibility to ischemic
            brain damage in hypertensive rats
  JOURNAL   J Cereb Blood Flow Metab 37 (8), 2780-2794 (2017)
   PUBMED   27798271
REFERENCE   2  (bases 1 to 4093)
  AUTHORS   Vorontsova,I., Lam,L., Delpire,E., Lim,J. and Donaldson,P.
  TITLE     Identification of the WNK-SPAK/OSR1 signaling pathway in rodent and
            human lenses
  JOURNAL   Invest Ophthalmol Vis Sci 56 (1), 310-321 (2014)
   PUBMED   25515571
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4093)
  AUTHORS   Ponce-Coria,J., Markadieu,N., Austin,T.M., Flammang,L., Rios,K.,
            Welling,P.A. and Delpire,E.
  TITLE     A novel Ste20-related proline/alanine-rich kinase
            (SPAK)-independent pathway involving calcium-binding protein 39
            (Cab39) and serine threonine kinase with no lysine member 4 (WNK4)
            in the activation of Na-K-Cl cotransporters
  JOURNAL   J Biol Chem 289 (25), 17680-17688 (2014)
   PUBMED   24811174
  REMARK    GeneRIF: in addition to SPAK and OSR1, WNK4 is able to anchor
            itself to the N-terminal domain of NKCC1 and to promote
            cotransporter activation.
REFERENCE   4  (bases 1 to 4093)
  AUTHORS   Shibata,S., Zhang,J., Puthumana,J., Stone,K.L. and Lifton,R.P.
  TITLE     Kelch-like 3 and Cullin 3 regulate electrolyte homeostasis via
            ubiquitination and degradation of WNK4
  JOURNAL   Proc Natl Acad Sci U S A 110 (19), 7838-7843 (2013)
   PUBMED   23576762
REFERENCE   5  (bases 1 to 4093)
  AUTHORS   Wakabayashi,M., Mori,T., Isobe,K., Sohara,E., Susa,K., Araki,Y.,
            Chiga,M., Kikuchi,E., Nomura,N., Mori,Y., Matsuo,H., Murata,T.,
            Nomura,S., Asano,T., Kawaguchi,H., Nonoyama,S., Rai,T., Sasaki,S.
            and Uchida,S.
  TITLE     Impaired KLHL3-mediated ubiquitination of WNK4 causes human
            hypertension
  JOURNAL   Cell Rep 3 (3), 858-868 (2013)
   PUBMED   23453970
REFERENCE   6  (bases 1 to 4093)
  AUTHORS   Kahle,K.T., Wilson,F.H., Leng,Q., Lalioti,M.D., O'Connell,A.D.,
            Dong,K., Rapson,A.K., MacGregor,G.G., Giebisch,G., Hebert,S.C. and
            Lifton,R.P.
  TITLE     WNK4 regulates the balance between renal NaCl reabsorption and K+
            secretion
  JOURNAL   Nat Genet 35 (4), 372-376 (2003)
   PUBMED   14608358
REFERENCE   7  (bases 1 to 4093)
  AUTHORS   Yang,C.L., Angell,J., Mitchell,R. and Ellison,D.H.
  TITLE     WNK kinases regulate thiazide-sensitive Na-Cl cotransport
  JOURNAL   J Clin Invest 111 (7), 1039-1045 (2003)
   PUBMED   12671053
REFERENCE   8  (bases 1 to 4093)
  AUTHORS   Monti,J., Zimdahl,H., Schulz,H., Plehm,R., Ganten,D. and Hubner,N.
  TITLE     The role of Wnk4 in polygenic hypertension: a candidate gene
            analysis on rat chromosome 10
  JOURNAL   Hypertension 41 (4), 938-942 (2003)
   PUBMED   12642508
  REMARK    GeneRIF: Gene analysis of rat chromosome 10 suggest that Wnk4 does
            not have a role in polygenic hypertension.
REFERENCE   9  (bases 1 to 4093)
  AUTHORS   Wilson,F.H., Kahle,K.T., Sabath,E., Lalioti,M.D., Rapson,A.K.,
            Hoover,R.S., Hebert,S.C., Gamba,G. and Lifton,R.P.
  TITLE     Molecular pathogenesis of inherited hypertension with hyperkalemia:
            the Na-Cl cotransporter is inhibited by wild-type but not mutant
            WNK4
  JOURNAL   Proc Natl Acad Sci U S A 100 (2), 680-684 (2003)
   PUBMED   12515852
REFERENCE   10 (bases 1 to 4093)
  AUTHORS   Wilson,F.H., Disse-Nicodeme,S., Choate,K.A., Ishikawa,K.,
            Nelson-Williams,C., Desitter,I., Gunel,M., Milford,D.V.,
            Lipkin,G.W., Achard,J.M., Feely,M.P., Dussol,B., Berland,Y.,
            Unwin,R.J., Mayan,H., Simon,D.B., Farfel,Z., Jeunemaitre,X. and
            Lifton,R.P.
  TITLE     Human hypertension caused by mutations in WNK kinases
  JOURNAL   Science 293 (5532), 1107-1112 (2001)
   PUBMED   11498583
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AY192567.1, AY187039.1 and BQ211864.1.
            
            On Oct 1, 2009 this sequence version replaced NM_175579.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY192567.1, AY187039.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132297, SAMEA5760383
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-54                AY192567.1         1-54
            55-4043             AY187039.1         32-4020
            4044-4093           BQ211864.1         1-50                c
FEATURES             Location/Qualifiers
     source          1..4093
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="10"
                     /map="10q31"
     gene            1..4093
                     /gene="Wnk4"
                     /gene_synonym="Ac2-059; Prkwnk4"
                     /note="WNK lysine deficient protein kinase 4"
                     /db_xref="GeneID:287715"
                     /db_xref="RGD:631401"
     exon            1..635
                     /gene="Wnk4"
                     /gene_synonym="Ac2-059; Prkwnk4"
                     /inference="alignment:Splign:2.1.0"
     CDS             27..3695
                     /gene="Wnk4"
                     /gene_synonym="Ac2-059; Prkwnk4"
                     /EC_number="2.7.11.1"
                     /note="WNK4 Ser/Thr kinase; protein kinase with no lysine
                     4; protein kinase lysine-deficient 4"
                     /codon_start=1
                     /product="serine/threonine-protein kinase WNK4"
                     /protein_id="NP_783169.2"
                     /db_xref="GeneID:287715"
                     /db_xref="RGD:631401"
                     /translation="MLAPRNTETGVHMSQTEADLALRPSPSLTSMGPTRLGPPPRRVR
                     RFSGKAEPRPRSSRPSRRSSVDLGLLSSWSQPASLLPEPPDPPDSAGPMRSPPSNSKE
                     HPEGTWTGAAPVKAADSACPELTVSSGGPGSREPPRVPDAAARERRREQEEKEDTETQ
                     AVATSPDGRYLKFDIEIGRGSFKTVYRGLDTDTTVEVAWCELQARKLSRAERQRFSEE
                     VEMLKGLQHPNIVRFYDSWKSVLRGQVCIVLVTELMTSGTLKTYLRRFREMKPRVLQR
                     WSRQILRGLHFLHSRVPPILHRDLKCDNVFITGPSGSVKIGDLGLATLKRASFAKSVI
                     GTPEFMAPEMYEEKYDEAVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTSGTKPN
                     SFYKVKMPEVKEIIEGCIRTDKNERFTIQDLLTHAFFREERGVHVELAEEDDGEKPGL
                     KLWLRMEDARRGGRPRDNQAIEFLFQLGRDAAEEVAQEMVALGLVCEADYQPVARAVR
                     ERVAAIQRKREKLRKARELEVLPPDSGPPPATVSMTPGPPSAFPPEPEEPEADQHQSF
                     LFRHASYSSTTSDCETDGYLSSSGFLDASDPALQPPGGMPSSPAEPHLCLPSGFALSI
                     PRSGPGSDFSPGDSYASDAASGLSDMGEGGQMRKNPVKTLRRRPRSRLRVTSVSDQSD
                     RVVECQLQTHNSKMVTFRFDLDGDSPEEIAAAMVYNEFILPSERDGFLSRIREIIQRV
                     ETLLKRDAGPSEATEDALSPQEEAAAMPALPGPSDEELQRSISPEQRSWAAFSTSPSS
                     PGTPLSPGTPFSPGTPPVFPCPIFPITSPSCHPYPFSQVSSNPCPQAPSSLLPSSSGA
                     SQVPFPSPSLPTSSPLPFSPSYPQVPLHPASLPTCPSPPPLPSTTAAPLLSLASAFSL
                     AVMTVAQSLLSPSPGLLSQSPPAPPGPLPSMPLPLASCDQESLSAQTAETENEASRNP
                     AQPLLGDARLAPISEEGKPQLVGRFQVTSSKEPAEPPLQPASPTLSRSLKLPTPQLTS
                     ESSDTEDSAAGGPETREALAESDRAAEGLGVAIDEEKDEGKEPQIGGSSPILSQPSPV
                     WMNYSYSSLCLSSEESESSGEDEEFWAELQNLRQKHLSEVEALQTLQKKEIEDLYSRL
                     GKQPPPGIVAPAAMLSCRQRRLSKGSFPTSRRNSLQRSDLPGPGIMRKNSLSGSSTGS
                     QEQRASKGVTFAGDVGRM"
     exon            636..808
                     /gene="Wnk4"
                     /gene_synonym="Ac2-059; Prkwnk4"
                     /inference="alignment:Splign:2.1.0"
     exon            809..1029
                     /gene="Wnk4"
                     /gene_synonym="Ac2-059; Prkwnk4"
                     /inference="alignment:Splign:2.1.0"
     exon            1030..1187
                     /gene="Wnk4"
                     /gene_synonym="Ac2-059; Prkwnk4"
                     /inference="alignment:Splign:2.1.0"
     exon            1188..1276
                     /gene="Wnk4"
                     /gene_synonym="Ac2-059; Prkwnk4"
                     /inference="alignment:Splign:2.1.0"
     exon            1277..1493
                     /gene="Wnk4"
                     /gene_synonym="Ac2-059; Prkwnk4"
                     /inference="alignment:Splign:2.1.0"
     exon            1494..1758
                     /gene="Wnk4"
                     /gene_synonym="Ac2-059; Prkwnk4"
                     /inference="alignment:Splign:2.1.0"
     exon            1759..1880
                     /gene="Wnk4"
                     /gene_synonym="Ac2-059; Prkwnk4"
                     /inference="alignment:Splign:2.1.0"
     exon            1881..1939
                     /gene="Wnk4"
                     /gene_synonym="Ac2-059; Prkwnk4"
                     /inference="alignment:Splign:2.1.0"
     exon            1940..2054
                     /gene="Wnk4"
                     /gene_synonym="Ac2-059; Prkwnk4"
                     /inference="alignment:Splign:2.1.0"
     exon            2055..2171
                     /gene="Wnk4"
                     /gene_synonym="Ac2-059; Prkwnk4"
                     /inference="alignment:Splign:2.1.0"
     exon            2172..2309
                     /gene="Wnk4"
                     /gene_synonym="Ac2-059; Prkwnk4"
                     /inference="alignment:Splign:2.1.0"
     exon            2310..2352
                     /gene="Wnk4"
                     /gene_synonym="Ac2-059; Prkwnk4"
                     /inference="alignment:Splign:2.1.0"
     exon            2353..2927
                     /gene="Wnk4"
                     /gene_synonym="Ac2-059; Prkwnk4"
                     /inference="alignment:Splign:2.1.0"
     exon            2928..2988
                     /gene="Wnk4"
                     /gene_synonym="Ac2-059; Prkwnk4"
                     /inference="alignment:Splign:2.1.0"
     exon            2989..3394
                     /gene="Wnk4"
                     /gene_synonym="Ac2-059; Prkwnk4"
                     /inference="alignment:Splign:2.1.0"
     exon            3395..3594
                     /gene="Wnk4"
                     /gene_synonym="Ac2-059; Prkwnk4"
                     /inference="alignment:Splign:2.1.0"
     exon            3595..3692
                     /gene="Wnk4"
                     /gene_synonym="Ac2-059; Prkwnk4"
                     /inference="alignment:Splign:2.1.0"
     exon            3693..4080
                     /gene="Wnk4"
                     /gene_synonym="Ac2-059; Prkwnk4"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ccgttctgcg gcgcccggcc cttcccatgc tagcacctcg aaatacagag accggggtcc
       61 acatgtccca aacggaggcc gacctggctt tgcggccatc tccgtctctc acctccatgg
      121 ggccgacccg cctggggcca cctcctcgcc gagtgcgccg cttctctggg aaggctgagc
      181 cccggccgcg ctcttcgaga ccgagccgcc gcagctcagt cgatctggga ctgttgagct
      241 cctggtctca accagcctca ctccttccgg aacctcctga tcctccggac tccgctggtc
      301 ccatgaggag cccaccttca aactctaaag aacaccccga gggcacatgg acgggggcag
      361 ccccggtgaa ggctgcagac tctgcctgtc ctgagcttac agtatcttcc ggaggtccgg
      421 ggtcccggga gccaccaagg gttcctgatg ctgcagcccg ggaacggcgg cgggagcagg
      481 aagaaaaaga ggacacagag acccaggctg tggcaacgtc tcccgacggc cgatacctca
      541 agtttgacat tgagattgga agaggttcct tcaagaccgt gtatcgaggg ctagacaccg
      601 acaccacggt agaggtggcc tggtgtgagc tgcaggctcg gaaactctct cgggctgaac
      661 ggcagcgttt ctctgaggaa gttgagatgc tcaaggggct gcagcacccc aacatcgtcc
      721 gcttctatga ctcctggaag tcggtgctga ggggtcaggt ttgcatcgtg ctggtcacag
      781 aactcatgac ctcgggcacg ctcaagacgt acctgaggag attccgagaa atgaagcccc
      841 gagtccttca gcgctggagt cgccagatcc tacggggact tcatttctta cattcccgag
      901 tgccccccat cctgcaccga gatctcaaat gtgacaatgt cttcattacc ggcccttcag
      961 gctctgtcaa aatcggagat ctcggactgg ccacgctcaa gcgcgcctcc tttgccaaga
     1021 gcgtcattgg gaccccggaa ttcatggccc ctgagatgta tgaggaaaag tacgacgagg
     1081 ctgtggatgt gtacgccttt ggcatgtgca tgctggagat ggctacgtct gagtatccct
     1141 actctgagtg tcagaacgca gcacaaatct accgaaaggt cacttcgggc acaaagccca
     1201 acagcttcta caaagtgaag atgccagagg tgaaggagat cattgaaggc tgcatccgca
     1261 cggataagaa tgagaggttc actatccagg atcttctgac ccacgcgttc ttccgcgagg
     1321 aacgtggtgt gcatgtggag ctggcagagg aagatgatgg agagaagccg ggtctcaaac
     1381 tctggctgcg catggaggac gcacgacgcg gggggcgccc aagggacaac caggccatcg
     1441 agttcctgtt ccagctcggt cgggatgcag cggaagaggt ggctcaggag atggtagcct
     1501 tgggcttagt ctgcgaagca gactaccagc cagtggcccg tgcagttcga gaacgggttg
     1561 ctgccatcca gcggaagcgg gagaagctaa gaaaagccag ggaattggag gttctcccac
     1621 cagactcagg acctcctcca gcaactgtgt ctatgactcc tggtcccccc agtgccttcc
     1681 ccccagagcc cgaggagcca gaggctgacc agcaccaatc cttcctcttc cgccatgcca
     1741 gctactcatc cactacatct gattgcgaga ctgatggcta cctcagctcc tccggcttcc
     1801 tggatgcctc agaccctgcc cttcagcccc ccggggggat gccatccagc cccgctgagc
     1861 cccatctctg cttgccctcg ggttttgcct tgtccattcc acggtctggc cctggcagtg
     1921 acttttctcc tggggacagc tacgcctcag atgcagcctc aggcctcagt gacatgggag
     1981 aaggggggca aatgaggaaa aatccagtga agactctgcg acggagacct cgatcccggc
     2041 ttcgggtcac gagtgtctca gaccagagcg acagagtcgt ggagtgccag ctgcaaactc
     2101 acaacagcaa gatggtgacg ttccgatttg atctggatgg ggacagccca gaagaaatcg
     2161 cagctgccat ggtttataat gaattcattc tgccctcgga gcgagacgga ttcctgagcc
     2221 ggatccggga gattatccag cgagtggaga ccctgctgaa gagagatgct ggtccctcgg
     2281 aggccactga agatgcgctg agccctcagg aagaggcagc agccatgcct gccctcccag
     2341 gcccatccga tgaagagctc cagagaagca tctccccgga acagaggagc tgggcagcct
     2401 tctctacatc tccatcttct cctggcaccc ccttgtcccc tggaaccccc ttttcccctg
     2461 ggacccctcc tgtcttccca tgccccatct tccctatcac ttcaccctca tgccatccct
     2521 acccgttctc acaggtctct tcaaatccct gtccacaggc ccccagctcc ctacttccct
     2581 cctcctccgg tgcttctcag gttccattcc catccccttc ccttcccacc agctctcccc
     2641 tcccattctc tcctagttat ccccaagtcc ctcttcaccc tgcttctctt cccacctgcc
     2701 cctcccctcc ccctctcccc tccaccacag cagcccctct cctctctctg gctagtgcct
     2761 tctctctggc tgtgatgact gtggcccagt ccctgctgtc cccatcccct gggcttctct
     2821 ctcagtctcc tccagcccct ccaggtcctc tgcctagcat gccccttccc cttgcttctt
     2881 gtgaccagga gagcctttca gcccaaacag ctgagacaga gaatgaggct tcccgaaatc
     2941 ctgctcagcc actactgggt gatgctagac tggcacccat atctgaagag ggaaaacccc
     3001 agctggttgg ccgattccaa gtgacttcat ccaaggaacc agcagagcct cccctacaac
     3061 cagcatctcc aactctctcc agatccctaa agcttccaac ccctcagctg acctcagaga
     3121 gttcagacac agaggacagt gctgcaggag gaccagagac cagggaggct ctggcagaga
     3181 gtgaccgtgc agccgagggc ttgggggtcg ccatcgatga ggaaaaggat gaagggaagg
     3241 aaccccaaat tgggggcagt tccccaatct tgagccagcc cagcccagtg tggatgaact
     3301 actcctacag cagcctgtgt ctaagcagtg aggagtcaga gagcagcggg gaggacgagg
     3361 agttctgggc tgagctgcag aaccttcggc agaagcactt gtcggaagtg gaagcactac
     3421 agacgctaca gaagaaggaa atcgaagact tgtacagccg gcttggaaag caacccccac
     3481 ctggtattgt agctccagcg gctatgctgt cctgccgcca gcgccgcctc tccaagggaa
     3541 gctttcccac ctcccgccgc aacagcctgc agcgctctga tctccctggc cctggcatca
     3601 tgcggaagaa ctctctgagt ggcagcagca ccggctccca ggagcagcgg gcaagcaagg
     3661 gggtgacatt cgccggggat gttggcagga tgtgaattca gaccagaagt cctatgtctc
     3721 cccaacacca gggccccacc atggcccgtg gcctctcaga atctgatgtt tttgtgacca
     3781 tctcaactca gcacagaaca ccccagcact gagggggtag aaggccagtg ggcatggaga
     3841 gtgcgttgtg ttatcctgaa gagtcaaact cgagggtgct ctttgctgtg tggaaatctc
     3901 atttccgtac ctaccccgcc ttcattttct gttcctcccc aggcaagggc ggaccactaa
     3961 gcaatcaaag tcaagtcctg atgcttgtag aaggccagct tccagctggg agagtgtagg
     4021 ggctgtgttc atgctaccaa agttaacagc aaccaataaa aatactgagg agaagaaaga
     4081 aaaaaaaaaa aaa
//
