LOCUS       NM_001411818           10613 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus WNK lysine deficient protein kinase 3 (Wnk3),
            transcript variant 1, mRNA.
ACCESSION   NM_001411818 XM_006256782
VERSION     NM_001411818.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 10613)
  AUTHORS   Zhu,J., Lin,X., Chen,C., Tan,H., Gao,Y., Li,D. and Chen,G.
  TITLE     WNK3 Promotes Neuronal Survival after Traumatic Brain Injury in
            Rats
  JOURNAL   Neuroscience 477, 76-88 (2021)
   PUBMED   34626749
  REMARK    GeneRIF: WNK3 Promotes Neuronal Survival after Traumatic Brain
            Injury in Rats.
REFERENCE   2  (bases 1 to 10613)
  AUTHORS   Wu,D., Lai,N., Deng,R., Liang,T., Pan,P., Yuan,G., Li,X., Li,H.,
            Shen,H., Wang,Z. and Chen,G.
  TITLE     Activated WNK3 induced by intracerebral hemorrhage deteriorates
            brain injury maybe via WNK3/SPAK/NKCC1 pathway
  JOURNAL   Exp Neurol 332, 113386 (2020)
   PUBMED   32589890
  REMARK    GeneRIF: Activated WNK3 induced by intracerebral hemorrhage
            deteriorates brain injury maybe via WNK3/SPAK/NKCC1 pathway.
REFERENCE   3  (bases 1 to 10613)
  AUTHORS   Zhang,J., Gao,G., Begum,G., Wang,J., Khanna,A.R., Shmukler,B.E.,
            Daubner,G.M., de Los Heros,P., Davies,P., Varghese,J.,
            Bhuiyan,M.I., Duan,J., Zhang,J., Duran,D., Alper,S.L., Sun,D.,
            Elledge,S.J., Alessi,D.R. and Kahle,K.T.
  TITLE     Functional kinomics establishes a critical node of volume-sensitive
            cation-Cl- cotransporter regulation in the mammalian brain
  JOURNAL   Sci Rep 6, 35986 (2016)
   PUBMED   27782176
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 10613)
  AUTHORS   Zhang,Z., Xu,X., Xiang,Z., Yu,Z., Feng,J. and He,C.
  TITLE     LINGO-1 receptor promotes neuronal apoptosis by inhibiting WNK3
            kinase activity
  JOURNAL   J Biol Chem 288 (17), 12152-12160 (2013)
   PUBMED   23482566
  REMARK    GeneRIF: LINGO-1 potentiates neuronal apoptosis, likely by
            inhibiting WNK3 kinase activity.
REFERENCE   5  (bases 1 to 10613)
  AUTHORS   Cruz-Rangel,S., Melo,Z., Vazquez,N., Meade,P., Bobadilla,N.A.,
            Pasantes-Morales,H., Gamba,G. and Mercado,A.
  TITLE     Similar effects of all WNK3 variants on SLC12 cotransporters
  JOURNAL   Am J Physiol Cell Physiol 301 (3), C601-C608 (2011)
   PUBMED   21613606
REFERENCE   6  (bases 1 to 10613)
  AUTHORS   Yang,C.L., Zhu,X. and Ellison,D.H.
  TITLE     The thiazide-sensitive Na-Cl cotransporter is regulated by a WNK
            kinase signaling complex
  JOURNAL   J Clin Invest 117 (11), 3403-3411 (2007)
   PUBMED   17975670
REFERENCE   7  (bases 1 to 10613)
  AUTHORS   Dorwart,M.R., Shcheynikov,N., Wang,Y., Stippec,S. and Muallem,S.
  TITLE     SLC26A9 is a Cl(-) channel regulated by the WNK kinases
  JOURNAL   J Physiol 584 (Pt 1), 333-345 (2007)
   PUBMED   17673510
REFERENCE   8  (bases 1 to 10613)
  AUTHORS   Verissimo,F., Silva,E., Morris,J.D., Pepperkok,R. and Jordan,P.
  TITLE     Protein kinase WNK3 increases cell survival in a
            caspase-3-dependent pathway
  JOURNAL   Oncogene 25 (30), 4172-4182 (2006)
   PUBMED   16501604
REFERENCE   9  (bases 1 to 10613)
  AUTHORS   Rinehart,J., Kahle,K.T., de Los Heros,P., Vazquez,N., Meade,P.,
            Wilson,F.H., Hebert,S.C., Gimenez,I., Gamba,G. and Lifton,R.P.
  TITLE     WNK3 kinase is a positive regulator of NKCC2 and NCC, renal
            cation-Cl- cotransporters required for normal blood pressure
            homeostasis
  JOURNAL   Proc Natl Acad Sci U S A 102 (46), 16777-16782 (2005)
   PUBMED   16275913
REFERENCE   10 (bases 1 to 10613)
  AUTHORS   Florea,L., Di Francesco,V., Miller,J., Turner,R., Yao,A.,
            Harris,M., Walenz,B., Mobarry,C., Merkulov,G.V., Charlab,R.,
            Dew,I., Deng,Z., Istrail,S., Li,P. and Sutton,G.
  TITLE     Gene and alternative splicing annotation with AIR
  JOURNAL   Genome Res 15 (1), 54-66 (2005)
   PUBMED   15632090
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000021.1.
            
            On Sep 2, 2022 this sequence version replaced XM_006256782.4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMEA5760383,
                              SAMEA5760393 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            RefSeq Select criteria    :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-171               JAXUCZ010000021.1  23599305-23599475
            172-823             JAXUCZ010000021.1  23634190-23634841
            824-996             JAXUCZ010000021.1  23645880-23646052
            997-1217            JAXUCZ010000021.1  23647817-23648037
            1218-1375           JAXUCZ010000021.1  23649490-23649647
            1376-1464           JAXUCZ010000021.1  23667847-23667935
            1465-1684           JAXUCZ010000021.1  23671142-23671361
            1685-1979           JAXUCZ010000021.1  23673614-23673908
            1980-2080           JAXUCZ010000021.1  23676622-23676722
            2081-2230           JAXUCZ010000021.1  23676954-23677103
            2231-2295           JAXUCZ010000021.1  23695535-23695599
            2296-2443           JAXUCZ010000021.1  23699301-23699448
            2444-2560           JAXUCZ010000021.1  23700993-23701109
            2561-2716           JAXUCZ010000021.1  23704515-23704670
            2717-2768           JAXUCZ010000021.1  23704751-23704802
            2769-2906           JAXUCZ010000021.1  23706078-23706215
            2907-3817           JAXUCZ010000021.1  23706552-23707462
            3818-4049           JAXUCZ010000021.1  23718398-23718629
            4050-4180           JAXUCZ010000021.1  23719015-23719145
            4181-4782           JAXUCZ010000021.1  23719971-23720572
            4783-4994           JAXUCZ010000021.1  23725595-23725806
            4995-5197           JAXUCZ010000021.1  23733134-23733336
            5198-10613          JAXUCZ010000021.1  23736875-23742290
FEATURES             Location/Qualifiers
     source          1..10613
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="X"
                     /map="Xq12"
     gene            1..10613
                     /gene="Wnk3"
                     /gene_synonym="RGD1563131"
                     /note="WNK lysine deficient protein kinase 3"
                     /db_xref="GeneID:317420"
                     /db_xref="RGD:1563131"
     exon            1..171
                     /gene="Wnk3"
                     /gene_synonym="RGD1563131"
                     /inference="alignment:Splign:2.1.0"
     exon            172..823
                     /gene="Wnk3"
                     /gene_synonym="RGD1563131"
                     /inference="alignment:Splign:2.1.0"
     CDS             290..5527
                     /gene="Wnk3"
                     /gene_synonym="RGD1563131"
                     /EC_number="2.7.11.1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     serine/threonine-protein kinase WNK3"
                     /codon_start=1
                     /product="serine/threonine-protein kinase WNK3 isoform 1"
                     /protein_id="NP_001398747.1"
                     /db_xref="GeneID:317420"
                     /db_xref="RGD:1563131"
                     /translation="MATDSGEPASTEDSEKPDGVSFQSRVARAVAPLTVEARIKEKYS
                     TFSASGETIERKRFFRKSVEMTEDDKVAQSSRRDERKVATNISRVEKVPTNVLRGGQE
                     VKYEQCSKATSESSKDCFKEKTEKEMEEEAEMKAVATSPSGRFLKFDIELGRGAFKTV
                     YKGLDTETWVEVAWCELQDRKLTKAEQQRFKEEAEMLKGLQHPNIVRFYDSWESILKG
                     KKCIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLQFLHTRTPPIIHRDLK
                     CDNIFITGPTGSVKIGDLGLATLMRTSFAKSVIGTPEFMAPEMYEEHYDESVDVYAFG
                     MCMLEMATSEYPYSECQNAAQIYRKVTSGIKPASFNKVTDPEVKEIIEGCIRQNKSER
                     LSIKDLLNHAFFAEDTGLRVELAEEDDCSNSSLALRLWVEDPKKLKGKHKDNEAIEFS
                     FNLEADTPEEVAYEMVKSGFFHESDSKAVAKSIRDRVTLIKKIREKKPAGCLEERRDS
                     QCKHARNVNPQQQTATLRPAPGPHNAAECEETEVDQHVRQQLVQGKAQQQPSSVRGDT
                     SSEPAAGPVLHSDTSSHPTVAYSSNQATSSQLQEQPKLTRSPVLPVVQGQSSVMPIYA
                     AGPAVASQSQISPLTIQKVSQIKPVSQPIGAEQQATPPNPDFVRSLNQDVTSVKENTN
                     SPDTPSGNGKQDRIKQRRASCPRPEKGTKFQLTVLQVSVSGDNMVECQLETHNNKMVT
                     FKFDVDGDAPEDIADYMVEDNFVLENEKEKFVEELRAIVGQAQEILHVHSAAEKSIGV
                     DSVALESNSNQTGSSEQVLINSASTQTSNESAPQSSPVGRWRFCINQTIKNREAQSPP
                     SLQPSVAMVPGLHPFPNNTSNQEISQDTLFTVPGHHVVFTSKLDGKIGERASVETEQT
                     SVPYQAEDDKLKAPATDTSNYSATLVCPDPAGCEALTSQAGMFIPTYPCQQAAVPADV
                     LMSHPGESVQIGSNAVVTSVLVSSDQKPQSLSVQQPTIDAEFISQEGETTVNSETSSP
                     KAVGATQTPGFEPTVLLPATILESDGERPPKMEFTDNRIKTLDEKLRNLLYQEHSIST
                     ICPESQKDTQSIDSPFSSSAEDILSSPMPEVIAISHCGIQDSQAQSPNFQPTGSKILS
                     NVAASQPAHIPVLKRDLNVITSVPSELCLHEMSPDASLPGDPEAYPAAVSSDGTIHLQ
                     TGGGYFGLSFTCPSLKNPISRKSWTRKLKSWAYRLRQSTSFFKRSKVRQVETEDKRSA
                     IASDPIPLTREFSADTRALSRCKAMSGSFQRGRFQVITVPQQQPVKMMSFGKEHRPPS
                     KKTTAQSNEQALTFTEAAVTQLIEVEPAIPTHKASVSSQKLRTLYETFKEDKGDPEQG
                     DILSLSTARETSVSSVTTEKNVEETSTTGISVQSGSEMLDKEKDESTPGKQTCTNEFS
                     ATPAGSGKSVAKAAPKSGQHLPARAQTQSSLFYSPSSPMSSDNESEIEDEDLKAELQR
                     LREKHIQEVVSLQTQQNKELQELYERLRATKDSKAQSSEVPMPPASPRRPRSFKSKLR
                     SRPQSLTHSDNLVVVKDVQRAESNTAPRQQSPASKKGMFTDDLHKLVDDWTRETVGNF
                     PSKPSLNQLKQSQQKSEAENWNKLHESTPSTVGYTSTWISSLSQIRGAAPSSLPHGLP
                     LPSFPGPLASYGMPHACQYNPVGAASYPVQWVGISGAAQQSVVLPTQSGGLFQPGMNL
                     QSFPAPPVQNPPSIPPGPK"
     exon            824..996
                     /gene="Wnk3"
                     /gene_synonym="RGD1563131"
                     /inference="alignment:Splign:2.1.0"
     exon            997..1217
                     /gene="Wnk3"
                     /gene_synonym="RGD1563131"
                     /inference="alignment:Splign:2.1.0"
     exon            1218..1375
                     /gene="Wnk3"
                     /gene_synonym="RGD1563131"
                     /inference="alignment:Splign:2.1.0"
     exon            1376..1464
                     /gene="Wnk3"
                     /gene_synonym="RGD1563131"
                     /inference="alignment:Splign:2.1.0"
     exon            1465..1684
                     /gene="Wnk3"
                     /gene_synonym="RGD1563131"
                     /inference="alignment:Splign:2.1.0"
     exon            1685..1979
                     /gene="Wnk3"
                     /gene_synonym="RGD1563131"
                     /inference="alignment:Splign:2.1.0"
     exon            1980..2080
                     /gene="Wnk3"
                     /gene_synonym="RGD1563131"
                     /inference="alignment:Splign:2.1.0"
     exon            2081..2230
                     /gene="Wnk3"
                     /gene_synonym="RGD1563131"
                     /inference="alignment:Splign:2.1.0"
     exon            2231..2295
                     /gene="Wnk3"
                     /gene_synonym="RGD1563131"
                     /inference="alignment:Splign:2.1.0"
     exon            2296..2443
                     /gene="Wnk3"
                     /gene_synonym="RGD1563131"
                     /inference="alignment:Splign:2.1.0"
     exon            2444..2560
                     /gene="Wnk3"
                     /gene_synonym="RGD1563131"
                     /inference="alignment:Splign:2.1.0"
     exon            2561..2716
                     /gene="Wnk3"
                     /gene_synonym="RGD1563131"
                     /inference="alignment:Splign:2.1.0"
     exon            2717..2768
                     /gene="Wnk3"
                     /gene_synonym="RGD1563131"
                     /inference="alignment:Splign:2.1.0"
     exon            2769..2906
                     /gene="Wnk3"
                     /gene_synonym="RGD1563131"
                     /inference="alignment:Splign:2.1.0"
     exon            2907..3817
                     /gene="Wnk3"
                     /gene_synonym="RGD1563131"
                     /inference="alignment:Splign:2.1.0"
     exon            3818..4049
                     /gene="Wnk3"
                     /gene_synonym="RGD1563131"
                     /inference="alignment:Splign:2.1.0"
     exon            4050..4180
                     /gene="Wnk3"
                     /gene_synonym="RGD1563131"
                     /inference="alignment:Splign:2.1.0"
     exon            4181..4782
                     /gene="Wnk3"
                     /gene_synonym="RGD1563131"
                     /inference="alignment:Splign:2.1.0"
     exon            4783..4994
                     /gene="Wnk3"
                     /gene_synonym="RGD1563131"
                     /inference="alignment:Splign:2.1.0"
     exon            4995..5197
                     /gene="Wnk3"
                     /gene_synonym="RGD1563131"
                     /inference="alignment:Splign:2.1.0"
     exon            5198..10613
                     /gene="Wnk3"
                     /gene_synonym="RGD1563131"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ccacgtgcct aggcctagcg gttacaggcc gtcaaaaatc ggttagctcc ctctcccatc
       61 ccttctttgg tgttgccccc cctcccccgc gcgcactgag gtcctggggg tggggggggc
      121 ggtggtggtg tatgccatgg ccgttgccct ctcaacataa ccaggagaca gattgctacg
      181 ggaatatatg actacataaa aggaagtgca ttctgatagt tctgatacct gagatgtaag
      241 tggactgaag agagaagcag gaaaaagttt agtgccaact ttagttacaa tggccactga
      301 ttctggggag ccagctagca cagaagattc tgagaagcct gatggagttt cctttcaaag
      361 cagggttgcc cgggctgttg cacctctgac tgtagaagct agaataaagg aaaaatacag
      421 taccttttct gcttctgggg aaaccattga aagaaagaga tttttccgaa agagtgttga
      481 gatgacagaa gatgacaaag ttgcccaatc ttcccgcaga gatgaaagaa aagttgcaac
      541 aaatatttca agagtagaaa aagttcctac aaatgtgcta agaggtgggc aagaagttaa
      601 atatgagcag tgttcaaagg caacctcaga atcctcaaaa gattgcttca aggagaaaac
      661 tgaaaaggaa atggaagaag aagcagaaat gaaggctgtc gcaacgtccc ctagtggcag
      721 gtttctaaaa tttgatatag agctgggaag aggcgctttt aaaacagtat ataaaggatt
      781 ggacactgaa acatgggttg aagttgcttg gtgtgagcta caggaccgca aattaactaa
      841 agctgagcag caacggttca aagaagaagc tgagatgttg aaaggcctcc agcatcccaa
      901 tatagttcga ttttacgatt catgggagtc catattgaaa ggaaagaaat gtattgtttt
      961 agtgactgaa ctaatgacat caggaacctt aaagacgtac ttaaaacgat ttaaagtcat
     1021 gaaaccaaag gtcttaagaa gctggtgcag gcaaatttta aaaggattgc agttcttaca
     1081 cacaaggacg cctcctatta ttcaccggga tctgaaatgt gacaacattt tcatcacagg
     1141 acccactgga tctgtgaaga ttggtgatct gggacttgca accttaatgc gcacatcctt
     1201 tgctaagagt gtcattggca ctcctgagtt tatggcgcca gagatgtatg aagaacacta
     1261 tgatgaatct gtagatgttt atgcttttgg gatgtgtatg ttggaaatgg ctacttcgga
     1321 gtatccatat tctgagtgcc agaatgcagc acaaatatac cgtaaagtaa ccagtggcat
     1381 aaaaccagcc agcttcaata aagtcacaga ccctgaagtg aaagaaataa ttgaaggatg
     1441 tattagacaa aacaaatctg aaaggttatc gatcaaggat ctactaaacc atgcattctt
     1501 tgctgaggat actggactga gggtggagtt agcagaggaa gatgattgct caaattcatc
     1561 cttggctcta agactctggg ttgaagatcc taagaaatta aaaggcaaac ataaagacaa
     1621 tgaagctatt gaattcagtt tcaacttaga agcagataca cctgaggaag tagcgtatga
     1681 aatggtcaag tctggattct tccatgaaag tgattccaaa gctgttgcta agtccattag
     1741 ggaccgtgtg acactgatta aaaagataag ggaaaagaag cctgctggat gcttagagga
     1801 acgcagggat tcccagtgca agcatgcgag gaatgtaaat cctcagcagc agactgcaac
     1861 tttacgacct gctcctggtc cacacaatgc ggctgagtgt gaagaaacag aagttgatca
     1921 acatgttcga cagcagcttg tacaaggcaa agcacagcag cagccctcct ctgttagggg
     1981 tgacacttca tctgaaccag cagctggacc agttctacat tcagatactt caagtcaccc
     2041 cactgtagcc tattcttcaa accaggcaac tagttctcag ttgcaggagc agccaaagct
     2101 gactcggtct ccagttttgc ctgtggttca aggtcagtct tctgttatgc ccatatatgc
     2161 tgctggacct gcagttgctt cacagtccca gatttctcca ttaactatcc agaaggtctc
     2221 acagataaag cctgtatccc aaccaattgg agctgaacaa caagcaactc ctccaaatcc
     2281 agattttgtt cgaagcttga atcaagatgt gacatctgtg aaagaaaaca ccaatagccc
     2341 tgatacccca agtggaaatg gcaaacagga tcggatcaaa caaagaagag cctcctgtcc
     2401 ccgaccagag aaggggacta aattccagct tactgtcctt caggtctcag tctctgggga
     2461 caacatggtg gaatgtcagc tggaaacaca taacaacaag atggtcacat tcaagtttga
     2521 tgttgatggt gatgcacctg aggatatcgc tgactatatg gttgaagata actttgtgct
     2581 ggaaaatgag aaagaaaaat ttgtagagga attgagggct atcgtaggtc aagcccagga
     2641 gatccttcat gtccactcag ctgcagaaaa atctattggt gttgattctg ttgccttgga
     2701 atctaacagt aaccaaacag gatcatctga acaagtattg ataaattctg catccactca
     2761 aactagcaat gaatctgctc ctcagtcatc cccagttggt cgctggcggt tttgtatcaa
     2821 ccaaacaata aaaaatcgtg aggcgcagtc tcctccttcc cttcagcctt ccgtggctat
     2881 ggtgcctggg ttacatccat ttcctaataa cacaagtaat caagaaatat cacaggacac
     2941 actgttcact gttccaggcc atcatgtagt tttcacctcc aagttagatg gtaagattgg
     3001 tgaacgtgct agtgtagaaa cggagcagac gagtgttccc tatcaagcag aagatgacaa
     3061 actaaaggca ccagctactg atacttccaa ttattctgct actttggtgt gtccagatcc
     3121 agctggatgt gaggcactca ccagccaagc aggcatgttc atacctacat atccatgtca
     3181 gcaagctgct gttccggctg atgtgcttat gtcccatcct ggtgaatcag ttcagattgg
     3241 cagtaatgct gttgtaacgt cagtacttgt atcttctgat caaaagcctc aatccttatc
     3301 agtacagcag ccaactatag atgcagagtt tatttcacaa gaaggagaaa ccacagtaaa
     3361 ctctgaaacg agttctccta aggctgtcgg tgccactcag actcctggct ttgaaccaac
     3421 tgtgcttcta cctgcgacta tcctggaatc agatggggaa cgacctccaa aaatggagtt
     3481 tacagacaat cgaattaaaa cactggatga aaaattaaga aacctgctct atcaagagca
     3541 cagcatttct accatctgtc ctgagagtca gaaggatact caaagcatag actctccatt
     3601 ttcttcctct gctgaagata tactatcctc cccaatgcca gaagtcatag ccatcagtca
     3661 ctgtgggatt caagatagcc aagcacaatc cccgaatttc caaccaacag gctctaagat
     3721 tctatcgaat gtggctgcaa gtcagcctgc tcatatacca gtgctcaaaa gagacctgaa
     3781 tgtgataact tctgtaccca gcgagctatg tttacacgag atgtccccag atgcttcact
     3841 tccaggggat ccagaggcct atcctgctgc tgtgtcaagc gatggaacca ttcatctgca
     3901 gacaggaggt ggatattttg gcctaagctt tacttgtcct agtctcaaaa atcctattag
     3961 caggaaatcc tggactcgaa aattaaaaag ctgggcatac aggctacggc agtcaaccag
     4021 ctttttcaag agatcaaaag ttcgtcaagt ggaaacagaa gataaaagat cagcaattgc
     4081 ttctgatccc attccgctga cacgggagtt ctcagctgat actagggctt tgagtagatg
     4141 taaagcgatg agtggatcat ttcagcgggg tcggttccag gtgattacag ttcctcagca
     4201 gcagccagtg aaaatgatgt cttttggaaa agaacataga ccgccatcga agaaaacgac
     4261 agcccagtct aacgagcaag cactaacctt tactgaagct gcagtgacac agttgatcga
     4321 agtggaacct gccataccca ctcataaagc ttcagtttct tcacaaaagt tacgaactct
     4381 ttatgaaacc tttaaagaag ataaaggaga ccctgaacaa ggtgacatct tatctttgag
     4441 cacagctcgt gagaccagtg tgtcttcagt gacaacagaa aagaatgtgg aagaaacttc
     4501 tactacagga attagtgtgc agtctgggtc tgaaatgtta gataaagaaa aagatgaatc
     4561 aactcctggg aaacagacat gtactaatga attttcagcc actcctgctg gcagtggaaa
     4621 gtcagtggca aaagctgctc caaagagtgg tcagcattta ccagcccgtg ctcaaacaca
     4681 gagttcactc ttctactctc catcttctcc aatgagcagt gacaatgaat cagaaatcga
     4741 ggatgaggac ttgaaagcgg agcttcaaag attacgggaa aaacacattc aagaggtggt
     4801 cagtcttcaa acacagcaga ataaagagct acaggagctc tatgaacgcc ttcgagcaac
     4861 taaagacagc aaagcgcaat cttcagaagt tcccatgcca cctgcatcac cacgcaggcc
     4921 aagatccttc aaaagcaaac ttcgaagccg cccccagtcc ttgacacatt cggacaatct
     4981 cgttgttgta aaagatgtgc agcgtgcgga gagtaatact gcgccacgcc agcaatcgcc
     5041 agccagtaag aaggggatgt tcacagatga tttacacaag ctggtggatg actggacaag
     5101 agaaacagta ggaaattttc ctagcaagcc aagtttaaac caactcaaac agagccaaca
     5161 aaaatcagag gcagaaaact ggaacaagtt gcatgaaagc actccatcta ctgtgggata
     5221 cacatcaacc tggatttctt ctctctccca aattcgcgga gctgccccgt cttccttgcc
     5281 acatggactc ccactccctt catttcctgg gccattagcg tcctatggaa tgcctcatgc
     5341 ttgtcagtac aatcccgtag gcgccgcaag ctatccagta cagtgggttg ggatttcggg
     5401 agcagcacag caatccgtag tgcttcctac ccaatctggg ggactcttcc agcctgggat
     5461 gaatctgcag tcatttccag ctccaccagt gcagaaccca ccttcaatcc ctcctggtcc
     5521 caaatgagtc caagtcagtg ctgaaggaag gggagagaac tttcagaaca cctgtgatgc
     5581 tatactttct tcttgagttc tgccatattt tttctcattc atctctactt taaactgtgt
     5641 tttttgttgt tctaatgtga tatttgatac agctcatcat tcggtagtaa tgtgcaattt
     5701 gcacaaagaa tactgcacac agaaatgttt tttcacttca aatcctatcc tctttgatac
     5761 ttgattttta aaaaatcctg ttccaaatgc tctactagct cagcttaagt gttaccattt
     5821 ccaagacacc tctcctgtgt gcggacatgc actcacctgc ccgccctctt ttttatctgc
     5881 taccagcagt tgtatgatac ttctctttaa catgtgttaa aaaaaaaagt atgtggattg
     5941 gttctcccca aaatgtggat gacgtaagac cctccaatag ccagtcttgt gggattcgtc
     6001 atgtctgtct taggtttact tttcacagaa ggcagtaact ggctgcagga tctgttttgg
     6061 tgtataaatg tagtagactt ttgtcaagca gtacaaaatc tgagtgtttt taaaatgtga
     6121 ttttctcccc gtcatcacag caggaggagt gagggcttgt ttggagtgag gcggagggaa
     6181 aggcaaaagc agaactgtaa cagttccttg aacctacagc taccagagct gcttctgggt
     6241 ttcagtcata acccaaagca gccacctttt ctcatgtgca caccgctttg gtttggagca
     6301 tccgcgaggc actggtttgg cttgcattct ttcgctaggc attgcagctg atgtgaatct
     6361 catttggggg ggtaatcctt gtaatcctaa ccaaggagat tcaggttaac acttctgtta
     6421 atccattctt ctaaagcgaa atgtacttga cacactaggg tttgtagctt ttgagacttt
     6481 tgcataaaac actggcttta cagggttcta agagatgtag gatacactgt cctgcagtga
     6541 actagtacac cctaggatga tattatttaa attgccttta accttttttg atgacaatgt
     6601 ttgggttgtt atcatttctt actagtgtct atagacttag aacattttag atcttagatg
     6661 cgtttgtcag cctcttgaga atttgagaga cttcactgtc aagttgcact ttactgggtt
     6721 ttatccagtt ttaggaggag ggaggcagtg tcaaaatgta cacctgtggt tttcaggaag
     6781 cttaactgta gtaaatctta cgtgctagtc acagggtcac ctaattttca gtctgtgaaa
     6841 cccaaataga atttttactt ggtatatatc tatatatcag ctcctctgta tacatgttca
     6901 aagacttatc attcactgaa gctttgaaag tcctttcaaa tacatgtttt cctatttagg
     6961 aattctcttg gtaaccattc caacttgtct tcgtttctct taacagattt tcttccatat
     7021 tcctctaaaa ccttaccccc atgggcttgc cgggaagtga ggaaacttaa tgggtgtgct
     7081 ttattttttc atacaatttc atactttgtg acattcagtg ccccttttaa aagttgtctt
     7141 atgttgctta cctaaagacc acacccatca tcacattttt gtctctgttc ctggggacat
     7201 tctgtggatt gctattgatt taagagccac ggtttctttt cctaagacat catgttttgg
     7261 ctgagtccag atcatatgca cggattcctc aaattccttc catggtattg ctttgcattt
     7321 gaagtgattt ttcggctact catgtagttc aagctagtca gttgcttgca ttgtagtgct
     7381 ttccacatgt acaaaacgat gctcctaaac ctcctggttc tttaaaactg tctgtttcat
     7441 taccagtcgg ttttgtgtaa ctatattggg ctagataacc tataaggtgg aatactacag
     7501 gtttaaagac tggctctcct cgagttgcaa tccttttaaa cagtgtgccc atggtgaaaa
     7561 ataattctac agatgacatg tacaacttca tttacaatct catggctgct ctacatagta
     7621 tgtcctgtgt tttttacaca tctcagtttt acatataact cattgtcctt gtttgcctgc
     7681 agatgtttgc agtgccttgt aatatttaga atcttttcag gttttgctgg aaagaaaagt
     7741 catcttctaa agtttgaaga tgggactagt gtactgctgt ctccatgctg actgcagtat
     7801 ctgggttaac atatatgtta catatacatt atgtatatgt aatgtgtaga agttaaagcg
     7861 tacaaaaaaa gggagtgtgc agaaatgaaa acaatgctat tcactttgct gtggggtttt
     7921 ctttactttc cctcattact ttttgatttg attcagattt tagtcacttt gattgtaaat
     7981 attgattagt tttctaggtg ggaattttgt attatttcat aactttgata agtcttataa
     8041 cagtgctgtt tccatccttg cctgaggtac ttttactccc ccatcaataa cgaattccag
     8101 ataacactca gaagtaccct tttctttaac aaagagccta gaacatataa taaaccttct
     8161 gttcctttct agaaagcgtg caaacctgtc ctttttgctt tctttcttgt gatcagcaca
     8221 aagcttgcac ttagccacca tcttgtaaag tttccttaag agatagtctg tatgtcccca
     8281 ttctgtttcc ctctcaagcg cacatctgat gagcagcatt gtgtcctcgt gtgtggtgac
     8341 atgaacacat ggccaggaag cactgctctt gtgtctgtgg agttggctaa aatataagac
     8401 tggaaccagt actgctgtct tcagtgcaga cccttcatat acctggagca gtcttgaatc
     8461 taagcaattt gctgtagttt cacaaatatc tctgcagttt ttcatctctt ccttgcttat
     8521 accaagcata agttctaata gtttgacagg aaacgtaact gtgaaagttt aaaagacact
     8581 tccgctgtga tttttgggtt gagtgtagtg ttttctaagg tctgatgcaa tgttatcatc
     8641 tgaaagcccc agatttactt gcagtccagt tacttttgtt tatctacctc gtttttcagt
     8701 actgttttag gtgcacaagt aaatgttgcc tgtgaaactt gcttttttgc cttgattctt
     8761 tgtgttagtt tagattctca atctggcttg ccttttttgt cctagcactc accatgcatg
     8821 tgggatgaga ggaaccatca ttctgccacc gatctgtgtt gaagcgatga cagccatgca
     8881 cctgtgtgta ataaagttag gggatttggg gatggaaagc aaagagagaa ataatttggt
     8941 ggcaatacag gacttagtgg tgggacttag ttttcaaatg tactgaggtg agacatcccc
     9001 agtgccccca acttcatatg tactggtgat gcttcctcac tcagtgccta aagggctatg
     9061 ggttgtaatt gctttaacta ccacagtgaa atacttgacc tcttagggca atgacccatc
     9121 ttcaaaatgt tagcagactg cctgctattt gcagtgacag tttgttatct taaaatggca
     9181 gaatgccttt catttgaatc atagttggta gtaagcgttc agataattgc ttatctaatc
     9241 tgtagtttct ggagttgact ctctttgctg tattttctaa gcattgaaaa ttctcttccc
     9301 ctccttaaaa aacttcagca aatgtttgta actggatatt ccttgtttca catgtgtact
     9361 gtgtaacagt acatttttaa tattagtaaa cactacacct cagtcatcat ttatatgtac
     9421 attcctgagg tagagttgcc atcagtttgt gattcggtta cctcatgttc tgtttgatga
     9481 agaaaaacct gagccttctg aggtggtatg caagccattt tctagaataa ctttttttta
     9541 atctgtagtc aatcagggac ttcttttccc tccctttatt tagaagatat aatagaaagg
     9601 tatgtatata tagaaaagac ttttgatttg gtaaataaaa ttttagggtt aaaaatttaa
     9661 aatctaaccc aaattatgaa aagaaaaccc tcgaacatag tatcagttcc ttttaaataa
     9721 aattagtgtg caattggtat ggagccatat gttcagattg ccctggaaag agtctctttt
     9781 tttgcgtgta tgaaggatga attcaaacat tgatcaggga ccttgaccac tactatgtga
     9841 atgggcccac agtagtaacc aggttaaatt attttccaat gagatggttg aaagagtgta
     9901 cagaccaaca gctccaattc tgggaggttc tctgaagtaa aacaacacag actgttaaaa
     9961 agctgaaagt caggtgagaa ggcaagggga agactatgct ctgaagggac aatgcttgga
    10021 aatgcacact tggcaccctt ctagcttttt gaccttgtgt ttctttccac tataatttaa
    10081 gatttaaggt tgcgatttgt agtctagacc cctcagcaga tagagtggat ataatgactg
    10141 gtcagaacgt gcagttgaag gtctgtcatg ctcaatcact taaagctata atgtgtttac
    10201 tgcgaatatt aagcatgtag acctagtgca gcatgggagc cactcaggaa gtttatgcaa
    10261 ttaaactttc atgcataatt taccatgaaa tatgtagatt tcagccaatt ctcaatactt
    10321 aacatttagt tatatatgtg aactctcctt tcttaattcg ggaatgtagc attatataga
    10381 atgctgttaa acttaatttt aaccttttta acattaactt ttgttttggt aagccaagtg
    10441 atctaccttt cagcagttgt ctggttttga ttcgtcgaaa ctgtgatttt tattttaatc
    10501 actacccaac tgttgttaaa tttttgtttc ctggaatttt gttttgaaat aaaaaataaa
    10561 ggctgtgtta aatgagtatg tcaccttatt aaaattctgt catatttact gca
//
