LOCUS       NP_001002823            2634 aa            linear   ROD 24-JUN-2024
DEFINITION  serine/threonine-protein kinase WNK1 isoform 1 [Rattus norvegicus].
ACCESSION   NP_001002823
VERSION     NP_001002823.2
DBSOURCE    REFSEQ: accession NM_001002823.2
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (residues 1 to 2634)
  AUTHORS   Boyd-Shiwarski,C.R., Shiwarski,D.J., Griffiths,S.E., Beacham,R.T.,
            Norrell,L., Morrison,D.E., Wang,J., Mann,J., Tennant,W.,
            Anderson,E.N., Franks,J., Calderon,M., Connolly,K.A., Cheema,M.U.,
            Weaver,C.J., Nkashama,L.J., Weckerly,C.C., Querry,K.E.,
            Pandey,U.B., Donnelly,C.J., Sun,D., Rodan,A.R. and Subramanya,A.R.
  TITLE     WNK kinases sense molecular crowding and rescue cell volume via
            phase separation
  JOURNAL   Cell 185 (24), 4488-4506 (2022)
   PUBMED   36318922
REFERENCE   2  (residues 1 to 2634)
  AUTHORS   Akella,R., Humphreys,J.M., Sekulski,K., He,H., Durbacz,M.,
            Chakravarthy,S., Liwocha,J., Mohammed,Z.J., Brautigam,C.A. and
            Goldsmith,E.J.
  TITLE     Osmosensing by WNK Kinases
  JOURNAL   Mol Biol Cell 32 (18), 1614-1623 (2021)
   PUBMED   33689398
  REMARK    GeneRIF: Osmosensing by WNK Kinases.
REFERENCE   3  (residues 1 to 2634)
  AUTHORS   Akella,R., Drozdz,M.A., Humphreys,J.M., Jiou,J., Durbacz,M.Z.,
            Mohammed,Z.J., He,H., Liwocha,J., Sekulski,K. and Goldsmith,E.J.
  TITLE     A Phosphorylated Intermediate in the Activation of WNK Kinases
  JOURNAL   Biochemistry 59 (18), 1747-1755 (2020)
   PUBMED   32314908
  REMARK    GeneRIF: A Phosphorylated Intermediate in the Activation of WNK
            Kinases.
REFERENCE   4  (residues 1 to 2634)
  AUTHORS   Kim,Y., Jun,I., Shin,D.H., Yoon,J.G., Piao,H., Jung,J., Park,H.W.,
            Cheng,M.H., Bahar,I., Whitcomb,D.C. and Lee,M.G.
  TITLE     Regulation of CFTR Bicarbonate Channel Activity by WNK1:
            Implications for Pancreatitis and CFTR-Related Disorders
  JOURNAL   Cell Mol Gastroenterol Hepatol 9 (1), 79-103 (2020)
   PUBMED   31561038
  REMARK    GeneRIF: Regulation of CFTR Bicarbonate Channel Activity by WNK1:
            Implications for Pancreatitis and CFTR-Related Disorders.
REFERENCE   5  (residues 1 to 2634)
  AUTHORS   Gao,J.L., Peng,K., Shen,M.W., Hou,Y.H., Qian,X.B., Meng,X.W.,
            Ji,F.H., Wang,L.N. and Yang,J.P.
  TITLE     Suppression of WNK1-SPAK/OSR1 Attenuates Bone Cancer Pain by
            Regulating NKCC1 and KCC2
  JOURNAL   J Pain 20 (12), 1416-1428 (2019)
   PUBMED   31085334
  REMARK    GeneRIF: Suppression of WNK1-SPAK/OSR1 Attenuates Bone Cancer Pain
            by Regulating NKCC1 and KCC2.
REFERENCE   6  (residues 1 to 2634)
  AUTHORS   Yang,C.L., Angell,J., Mitchell,R. and Ellison,D.H.
  TITLE     WNK kinases regulate thiazide-sensitive Na-Cl cotransport
  JOURNAL   J Clin Invest 111 (7), 1039-1045 (2003)
   PUBMED   12671053
  REMARK    GeneRIF: WNK1 has a role in the sodium regulatory pathway of the
            distal
REFERENCE   7  (residues 1 to 2634)
  AUTHORS   Xu,B.E., Min,X., Stippec,S., Lee,B.H., Goldsmith,E.J. and Cobb,M.H.
  TITLE     Regulation of WNK1 by an autoinhibitory domain and
            autophosphorylation
  JOURNAL   J Biol Chem 277 (50), 48456-48462 (2002)
   PUBMED   12374799
REFERENCE   8  (residues 1 to 2634)
  AUTHORS   Wilson,F.H., Disse-Nicodeme,S., Choate,K.A., Ishikawa,K.,
            Nelson-Williams,C., Desitter,I., Gunel,M., Milford,D.V.,
            Lipkin,G.W., Achard,J.M., Feely,M.P., Dussol,B., Berland,Y.,
            Unwin,R.J., Mayan,H., Simon,D.B., Farfel,Z., Jeunemaitre,X. and
            Lifton,R.P.
  TITLE     Human hypertension caused by mutations in WNK kinases
  JOURNAL   Science 293 (5532), 1107-1112 (2001)
   PUBMED   11498583
REFERENCE   9  (residues 1 to 2634)
  AUTHORS   Xu,B., English,J.M., Wilsbacher,J.L., Stippec,S., Goldsmith,E.J.
            and Cobb,M.H.
  TITLE     WNK1, a novel mammalian serine/threonine protein kinase lacking the
            catalytic lysine in subdomain II
  JOURNAL   J Biol Chem 275 (22), 16795-16801 (2000)
   PUBMED   10828064
REFERENCE   10 (residues 1 to 2634)
  AUTHORS   Moore,T.M., Garg,R., Johnson,C., Coptcoat,M.J., Ridley,A.J. and
            Morris,J.D.
  TITLE     PSK, a novel STE20-like kinase derived from prostatic carcinoma
            that activates the c-Jun N-terminal kinase mitogen-activated
            protein kinase pathway and regulates actin cytoskeletal
            organization
  JOURNAL   J Biol Chem 275 (6), 4311-4322 (2000)
   PUBMED   10660600
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AF227741.1 and JAXUCZ010000004.1.
            
            On Nov 20, 2010 this sequence version replaced NP_001002823.1.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1). This variant
            represents the exon combination of the dorsal root ganglia and
            sciatic nerve variant inferred from the mouse sequence described in
            Figure 2F of PubMed ID 18521183.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMEA5760383, SAMEA5760386
                              [ECO:0006172]
            ##Evidence-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Rattus norvegicus"
                     /db_xref="taxon:10116"
                     /chromosome="4"
                     /map="4q42"
     Protein         1..2634
                     /product="serine/threonine-protein kinase WNK1 isoform 1"
                     /EC_number="2.7.11.1"
                     /note="protein kinase with no lysine 1"
                     /calculated_mol_wt=279436
     Region          1..80
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9JIH7.2)"
     Site            17
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q9H4A3; propagated from
                     UniProtKB/Swiss-Prot (Q9JIH7.2)"
     Site            58
                     /site_type="phosphorylation"
                     /note="Phosphothreonine.
                     /evidence=ECO:0000269|PubMed:16081417; propagated from
                     UniProtKB/Swiss-Prot (Q9JIH7.2)"
     Region          93..202
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9JIH7.2)"
     Site            165
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (Q9JIH7.2)"
     Site            172
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (Q9JIH7.2)"
     Region          195..483
                     /region_name="STKc_WNK1"
                     /note="Catalytic domain of the Serine/Threonine protein
                     kinase, With No Lysine (WNK) 1; cd14030"
                     /db_xref="CDD:270932"
     Site            order(227..231,233,235,248,250,281,301..304,308,310,349,
                     351,353..354,356,368,371,385..388)
                     /site_type="active"
                     /db_xref="CDD:270932"
     Site            order(227..231,233,235,248,250,281,301..304,308,349,351,
                     353..354,356,368)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270932"
     Site            order(231,308,310,349,351,353,371,385..388)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270932"
     Site            367..388
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270932"
     Site            378
                     /site_type="phosphorylation"
                     /note="Phosphoserine, by autocatalysis.
                     /evidence=ECO:0000269|PubMed:12374799; propagated from
                     UniProtKB/Swiss-Prot (Q9JIH7.2)"
     Site            382
                     /site_type="phosphorylation"
                     /note="Phosphoserine, by autocatalysis.
                     /evidence=ECO:0000269|PubMed:12374799,
                     ECO:0000269|PubMed:22544747; propagated from
                     UniProtKB/Swiss-Prot (Q9JIH7.2)"
     Region          488..555
                     /region_name="Autoinhibitory domain.
                     /evidence=ECO:0000269|PubMed:12374799"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9JIH7.2)"
     Region          500..563
                     /region_name="OSR1_C"
                     /note="Oxidative-stress-responsive kinase 1 C-terminal
                     domain; pfam12202"
                     /db_xref="CDD:463491"
     Region          629..639
                     /region_name="Interaction with KLHL3.
                     /evidence=ECO:0000250|UniProtKB:Q96J92"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9JIH7.2)"
     Region          <1828..2116
                     /region_name="PHA03247"
                     /note="large tegument protein UL36; Provisional"
                     /db_xref="CDD:223021"
     CDS             1..2634
                     /gene="Wnk1"
                     /gene_synonym="Hsn2; Prkwnk1"
                     /coded_by="NM_001002823.2:166..8070"
                     /note="isoform 1 is encoded by transcript variant 1"
                     /db_xref="GeneID:116477"
                     /db_xref="RGD:621141"
ORIGIN      
        1 msdgtaekqs gtpgflsppa pvpkngsssd ssvgeklgaa vadsgigrte eyrrrrhtmd
       61 kdsrgaaatt tptehrffrr svicdsnata lelpglplsi pqpsvpavvp qsappephre
      121 etltatvasq vsqqpsaaas pgeqavvgsa tatvpsstsk drpvsqpslv gskeepppsr
      181 sgsgsggasa kepqeernqq qddieeletk avgmsndgrf lkfdieigrg sfktvykgld
      241 tettvevawc elqdrkltks erqrfkeeae mlkglqhpni vrfydswest vkgkkcivlv
      301 telmtsgtlk tylkrfkvmk ikvlrswcrq ilkglqflht rtppiihrdl kcdnifitgp
      361 tgsvkigdlg latlkrasfa ksvigtpefm apemyeekyd esvdvyafgm cmlematsey
      421 pysecqnaaq iyrrvtsgvk pasfdkvaip evkeiiegci rqnkderysi kdllnhaffq
      481 eetgvrvela eeddgekiai klwlriedik klkgkykdne aiefsfdler dvpedvaqem
      541 vesgyvcegd hktmakaikd rvslikrkre qrqlvreeqe krkqeessfk qqneqqasvs
      601 qagiqplsva stgiptaptt sasvstqvep eepeadqhqq lqyqqpsisv lsdgtvdsgq
      661 gssvftesrv ssqqtvsygs qheqahsigt apghtvssiq aqsqphgvyp pssmpqsvah
      721 pcggtptype sqiffptihe rpvsfspppt cppkvaisqr rkstsfleaq trhfqpllrt
      781 vgqnhlppgg sptnwtpeai vmlgttanrv nrelcemqvq pvfettqiys dyrpglvlae
      841 eahyfipqet vylagvhyqa haagqyegis ynspvlsspm kqiteqkpvp gcpasssvfe
      901 fpsgqaflvg hlqnlrldsg pspasplssi sapnstdath lkfhpvfvph sapavlthsn
      961 enrsncvfef haqtpssssg egggilpqrv yrnrqvavds sqeelspqsv glhchlqpvt
     1021 eeqrnnhtpe ltisvvepmg qnwpvgspey ssdssqitss disdfqsppp tggtaapfgs
     1081 dvslpyirlp qtvlqesplf fcfpqgttsq qvlsasyssg gsalhpqaqg qnqgqpsssl
     1141 agvlssqpvq hpqqqgiqpt vppqqavqys lpqaassseg tvqpvsqpqv sagtqgfpsr
     1201 lppqypgdsn iapssnvasv cihstvlapp pmptealatq gyfptvvqpy vestplvpmg
     1261 svggqvqvsq pavslsqqpp ttssqqavle sstqgvsqaa ppeqtpitqs qptqpvplvs
     1321 svdsahsdva sgmsdgnena psssgrhegr ttkrhyrksv rsrsrhekts rpklrilnvs
     1381 nkgdrvvecq lethnrkmvt fkfdldgdnp eeiatimvnn dfilaieres fvaqvreiie
     1441 kademlsedv svepegdqgl eslqgkddyg fpgsqklege fkqpiavssm pqqigvptss
     1501 ltqvvhsagr rfivspvpes rlreskifts eipdpvaast sqgpgmnlsh sasslslqqa
     1561 fselkhgqmt egpntappnf nhpgptfspf ltsiagvqtv aastpsvsvp itssplndis
     1621 tsvmqsegal ptdkgiggvt tstgvvasgg lttlsvsetp tlssavssst apavvtvstt
     1681 sqpvqastsg siasstgsfp sgtfstttgt tvssvavpna kpptvllqqv agntagvaiv
     1741 tsvstttpfp amasqpslpl gsstsaptla etvvvsahsl dkashsstag lglsfcapss
     1801 ssssgtavss svsqpgivhp lvissaiast pvlpqpavpt stpllpqvpn ipplvqpvan
     1861 vpavqqtlih sqpqpallpn qphthcpemd adtqskapgi ddiktleekl rslfsehsss
     1921 gtqhasvsle tplvvetvtp gipttavaps klmtsttstc lpptnlplgt agmpvmpvgt
     1981 pgqvstpgth asapastatg akpgttppkp sltktvvppv gtelsagtvp ceqlppfpgp
     2041 sliqtqqple dldaqlrrtl spetipvtpa vgplstmsst avteagsqpq kdgtevhvta
     2101 sssgagvvkm grfqvsvtmd daqkerknrs edtksvhfes stsessvlss sspestlvkp
     2161 epngitvsgi sldvpdsthr tptpeakset gqptkvgrfq vtttankvgr fsvsrtedkv
     2221 telkkegpvt spfrdseqtv ipaaipkkek pelaepshln gpssdleaaf lsrggedgsg
     2281 sphspphlcs kslpiqtlsq slsnsfnssy mssdnesdie dedlrlelrr lrekhlkeiq
     2341 dlqsrqkhei eslytklgkv ppaviippaa plsgrrrrpt kskgskssrs sslgnkspql
     2401 sgnlsgqsgt svlnpqqtlh ppgntpetgh nqllqplkps pssdnlysaf tsdgaisvps
     2461 lsapgqgtss tntvggtvss qaaqaqppam tssrkgtftd dlhklvdnwa rdamnlsgrr
     2521 gskghmnyeg pgmarkfsap gqlcismtsn mggstpisaa satslghftk smcppqqygf
     2581 paapfgtqws gtggpapqpl gqfqpvgtts lqnfnisnlq ksisnppgsn lrtt
//
