LOCUS       NM_001085353            2640 bp    mRNA    linear   ROD 03-JUL-2024
DEFINITION  Rattus norvegicus Wnt ligand secretion mediator (Wls), transcript
            variant 2, mRNA.
ACCESSION   NM_001085353
VERSION     NM_001085353.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Tacelosky,D.M., Alexander,D.N., Morse,M., Hajnal,A., Berg,A.,
            Levenson,R. and Grigson,P.S.
  TITLE     Low expression of D2R and Wntless correlates with high motivation
            for heroin
  JOURNAL   Behav Neurosci 129 (6), 744-755 (2015)
   PUBMED   26501177
  REMARK    GeneRIF: Results link reduced expression of both the D2R and
            Wntless to the explicit motivation for heroin rather than to
            differences in total drug intake per se
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Bian,W.J., Miao,W.Y., He,S.J., Wan,Z.F., Luo,Z.G. and Yu,X.
  TITLE     A novel Wnt5a-Frizzled4 signaling pathway mediates
            activity-independent dendrite morphogenesis via the distal PDZ
            motif of Frizzled 4
  JOURNAL   Dev Neurobiol 75 (8), 805-822 (2015)
   PUBMED   25424568
REFERENCE   3  (bases 1 to 2640)
  AUTHORS   Yu,J., Chia,J., Canning,C.A., Jones,C.M., Bard,F.A. and
            Virshup,D.M.
  TITLE     WLS retrograde transport to the endoplasmic reticulum during Wnt
            secretion
  JOURNAL   Dev Cell 29 (3), 277-291 (2014)
   PUBMED   24768165
REFERENCE   4  (bases 1 to 2640)
  AUTHORS   Jaremko,K.M., Thompson,N.L. Jr., Reyes,B.A., Jin,J., Ebersole,B.,
            Jenney,C.B., Grigson,P.S., Levenson,R., Berrettini,W.H. and Van
            Bockstaele,E.J.
  TITLE     Morphine-induced trafficking of a mu-opioid receptor interacting
            protein in rat locus coeruleus neurons
  JOURNAL   Prog Neuropsychopharmacol Biol Psychiatry 50, 53-65 (2014)
   PUBMED   24333843
REFERENCE   5  (bases 1 to 2640)
  AUTHORS   Das,S., Yu,S., Sakamori,R., Stypulkowski,E. and Gao,N.
  TITLE     Wntless in Wnt secretion: molecular, cellular and genetic aspects
  JOURNAL   Front Biol (Beijing) 7 (6), 587-593 (2012)
   PUBMED   23439944
REFERENCE   6  (bases 1 to 2640)
  AUTHORS   Belenkaya,T.Y., Wu,Y., Tang,X., Zhou,B., Cheng,L., Sharma,Y.V.,
            Yan,D., Selva,E.M. and Lin,X.
  TITLE     The retromer complex influences Wnt secretion by recycling wntless
            from endosomes to the trans-Golgi network
  JOURNAL   Dev Cell 14 (1), 120-131 (2008)
   PUBMED   18160348
REFERENCE   7  (bases 1 to 2640)
  AUTHORS   Binnerts,M.E., Kim,K.A., Bright,J.M., Patel,S.M., Tran,K., Zhou,M.,
            Leung,J.M., Liu,Y., Lomas,W.E. 3rd, Dixon,M., Hazell,S.A.,
            Wagle,M., Nie,W.S., Tomasevic,N., Williams,J., Zhan,X., Levy,M.D.,
            Funk,W.D. and Abo,A.
  TITLE     R-Spondin1 regulates Wnt signaling by inhibiting internalization of
            LRP6
  JOURNAL   Proc Natl Acad Sci U S A 104 (37), 14700-14705 (2007)
   PUBMED   17804805
REFERENCE   8  (bases 1 to 2640)
  AUTHORS   Bartscherer,K., Pelte,N., Ingelfinger,D. and Boutros,M.
  TITLE     Secretion of Wnt ligands requires Evi, a conserved transmembrane
            protein
  JOURNAL   Cell 125 (3), 523-533 (2006)
   PUBMED   16678096
REFERENCE   9  (bases 1 to 2640)
  AUTHORS   Banziger,C., Soldini,D., Schutt,C., Zipperlen,P., Hausmann,G. and
            Basler,K.
  TITLE     Wntless, a conserved membrane protein dedicated to the secretion of
            Wnt proteins from signaling cells
  JOURNAL   Cell 125 (3), 509-522 (2006)
   PUBMED   16678095
REFERENCE   10 (bases 1 to 2640)
  AUTHORS   Ahmed,F., Torrado,M., Zinovieva,R.D., Senatorov,V.V., Wistow,G. and
            Tomarev,S.I.
  TITLE     Gene expression profile of the rat eye iridocorneal angle: NEIBank
            expressed sequence tag analysis
  JOURNAL   Invest Ophthalmol Vis Sci 45 (9), 3081-3090 (2004)
   PUBMED   15326124
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC062391.1.
            
            Transcript Variant: This variant (2) differs in the 3' UTR compared
            to variant 1. Variants 1 and 2 encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC062391.1, SRR8487231.33401.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132262
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="2"
                     /map="2q45"
     gene            1..2640
                     /gene="Wls"
                     /gene_synonym="Gpr177"
                     /note="Wnt ligand secretion mediator"
                     /db_xref="GeneID:362065"
                     /db_xref="RGD:735139"
     exon            1..287
                     /gene="Wls"
                     /gene_synonym="Gpr177"
                     /inference="alignment:Splign:2.1.0"
     CDS             182..1807
                     /gene="Wls"
                     /gene_synonym="Gpr177"
                     /note="integral membrane protein GPR177; EVI; protein
                     evenness interrupted homolog; G protein-coupled receptor
                     177; wntless Wnt ligand secretion mediator"
                     /codon_start=1
                     /product="protein wntless homolog"
                     /protein_id="NP_001078822.1"
                     /db_xref="GeneID:362065"
                     /db_xref="RGD:735139"
                     /translation="MAGAIIENMSTKKLCIVGGILLVFQIVAFLVGGLIAPAPTTAVS
                     YVAAKCVDVRKNHHKTRWLMPWGPNKCNKINDFEEAIPREIEANDIVFSVHIPLPSME
                     MSPWFQFMLFILQIDIAFKLNNQIRENAEVSMDVSLGYRDDMFSEWTEMAHERVPRKL
                     RCTFTSPKTPEHEGRHYECDVLPFMEIGSVAHKYYLLNIRLPVNEKKKINVGIGEIKD
                     IRLVGIHQNGGFTKVWFAMKTFLTPSIFIIMVWYWRRITMMSRPPVLLEKVIFALGIS
                     MTFINIPVEWFSIGFDWTWMLLFGDIRQGIFYAMLLSFWIIFCGEHMMDQHERNHIAG
                     YWKQVGPIAVGSFCLFIFDMCERGVQLTNPFYSIWTTDVGTELAMAFIIVAGICLCLY
                     FLFLCFMVFQVFRNISGKQSSLPAMSKVRRLHYEGLIFRFKFLMLITLACAAMTVIFF
                     IVSQVTEGHWKWGGVTVQVSSAFFTGIYGMWNLYVFALMFLYAPSHKNYGEDQSNGDL
                     GVHSGEELQLTTTITHVDGPTEIYKLTRKEAQE"
     exon            288..560
                     /gene="Wls"
                     /gene_synonym="Gpr177"
                     /inference="alignment:Splign:2.1.0"
     exon            561..685
                     /gene="Wls"
                     /gene_synonym="Gpr177"
                     /inference="alignment:Splign:2.1.0"
     exon            686..847
                     /gene="Wls"
                     /gene_synonym="Gpr177"
                     /inference="alignment:Splign:2.1.0"
     exon            848..984
                     /gene="Wls"
                     /gene_synonym="Gpr177"
                     /inference="alignment:Splign:2.1.0"
     exon            985..1153
                     /gene="Wls"
                     /gene_synonym="Gpr177"
                     /inference="alignment:Splign:2.1.0"
     exon            1154..1251
                     /gene="Wls"
                     /gene_synonym="Gpr177"
                     /inference="alignment:Splign:2.1.0"
     exon            1252..1315
                     /gene="Wls"
                     /gene_synonym="Gpr177"
                     /inference="alignment:Splign:2.1.0"
     exon            1316..1459
                     /gene="Wls"
                     /gene_synonym="Gpr177"
                     /inference="alignment:Splign:2.1.0"
     exon            1460..1543
                     /gene="Wls"
                     /gene_synonym="Gpr177"
                     /inference="alignment:Splign:2.1.0"
     exon            1544..1697
                     /gene="Wls"
                     /gene_synonym="Gpr177"
                     /inference="alignment:Splign:2.1.0"
     exon            1698..2618
                     /gene="Wls"
                     /gene_synonym="Gpr177"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gggaacccaa gcggcttcgc ccaggcattc gcgcgggcgc ccgcggtctg ggtcccacct
       61 cctctgcttt cgcacccttg aagttttgga gcaccaggaa aagagggcaa ggaaggagag
      121 gggaagcgaa agcatatcct aaaacattta cttaaaggag gaaagaaaag gggtcgcaga
      181 aatggctggg gcaattatag aaaacatgag caccaagaag ctctgcattg ttggagggat
      241 tcttctggtt ttccaaatcg ttgcctttct ggtgggaggc ttgatcgctc cagcacccac
      301 aacggcagtg tcctacgtgg cagcaaaatg tgtggatgtc cggaagaacc accataaaac
      361 aagatggctg atgccctggg gaccaaacaa gtgtaacaag atcaatgact tcgaagaagc
      421 aattccaagg gaaattgaag cgaatgacat tgtgttttct gtacacattc ccctcccttc
      481 tatggagatg agcccatggt tccagtttat gctgtttatc ctgcagatag acattgcttt
      541 caagctaaac aaccaaatca gagaaaatgc agaagtttcc atggatgttt ccctgggtta
      601 ccgtgatgat atgttttctg agtggactga aatggcgcac gaaagagtac cacgtaaact
      661 cagatgcact ttcacatccc ccaagacccc agagcatgaa ggtcgtcatt atgaatgtga
      721 tgtccttcct ttcatggaaa ttgggtcagt ggctcataag tattaccttc taaatatccg
      781 gctacctgta aatgagaaga agaaaatcaa tgttggaatt ggggaaataa aggacattcg
      841 gttggtggga atccaccaaa atggaggttt cactaaggta tggtttgcta tgaagacctt
      901 cctcacaccc agcatcttca tcattatggt gtggtattgg agaaggatca ccatgatgtc
      961 ccgacctcca gtgcttctgg aaaaagtcat ctttgccctt gggatttcca tgacctttat
     1021 caatatccct gtggaatggt tttccattgg atttgattgg acctggatgc tgttatttgg
     1081 tgacatacga cagggcatct tctatgcaat gcttctttcc ttctggatca tcttctgtgg
     1141 cgagcacatg atggatcaac atgagcggaa tcacattgca gggtattgga agcaagttgg
     1201 accaattgct gttggctctt tctgcctctt catatttgac atgtgtgaga gaggagtgca
     1261 actcacaaat cctttctaca gtatctggac tacagatgtt ggaacagaac tggctatggc
     1321 tttcatcatt gtggcaggta tctgcctctg cctctacttc ctgtttctgt gtttcatggt
     1381 atttcaagta ttcagaaaca tcagtgggaa acagtctagc ctcccagcca tgagcaaagt
     1441 ccggaggctg cactatgagg gtctgatttt caggttcaag ttcctcatgc tgatcacctt
     1501 ggcttgtgct gccatgactg ttatcttctt cattgttagt caggtgacag aaggccattg
     1561 gaaatggggt ggggtcacag ttcaagtgag cagtgctttc ttcactggaa tctatgggat
     1621 gtggaacctg tatgtctttg ctttgatgtt cttgtatgca ccatcccata agaactatgg
     1681 ggaagaccag tctaatggtg acctgggtgt ccacagcggg gaagaactgc agctcactac
     1741 cacaatcacc catgtagatg gaccgactga gatctacaag ttgacccgta aagaagcaca
     1801 ggagtagtag gctatggtat ggcactgggc tggaattgtc tctctgtcca gcctcttccc
     1861 ctggaaaaga tgtcctacta tacagagagc agtgaaaagt gaatgcctaa tgcttcttag
     1921 gtatagtttc ttagaacaat ggatggacat ttgtcagatg acctccaatg gtggctgttg
     1981 taagacttct gcctatgtag catatgataa caaaaactga tatgacacat tttttctgtg
     2041 atcattgtta attagtgaca tagcaacatc tgtagcagct gggttaataa cccttacata
     2101 gagggatata taggatggtt tgggccaata ggcattatat tgagtagatt atggttattt
     2161 ttttctgttt tagattctgg gatatgttta aacattcttt gcaggccaaa catttgctga
     2221 tgtcatagca ttttcactct cctccatggg cacttttttt ctatttatat caccaagtag
     2281 ccaacagagt tattcaagtt gtcagtacaa atatatccac tttttaaaaa atggattact
     2341 ataaaataac caaacttcat gagaaattct atcttaccag aggcatttca gcttttaaac
     2401 caaatctgtg tatttgatac taatctgtct gttggatgtg gattttgaga aatatttgct
     2461 aaattaccca aataggattt cttttattaa atggcctttg ggtcctaaaa agctttttaa
     2521 acttcttgct tgaaatgtgt tttcttttga taagatgttt caaacagcct ccaaaactgt
     2581 aaatccaatc atttgaataa aatgaatgta aacatgcaaa aaaaaaaaaa aaaaaaaaaa
//
