LOCUS       NM_001110365            4355 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Rattus norvegicus WASP like actin nucleation promoting factor
            (Wasl), mRNA.
ACCESSION   NM_001110365 XM_001061794
VERSION     NM_001110365.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 4355)
  AUTHORS   Bouslama,R., Dumont,V., Lindfors,S., Paavolainen,L., Tienari,J.,
            Nisen,H., Mirtti,T., Saleem,M.A., Gordin,D., Groop,P.H.,
            Suetsugu,S. and Lehtonen,S.
  TITLE     Phosphorylation of PACSIN2 at S313 Regulates Podocyte Architecture
            in Coordination with N-WASP
  JOURNAL   Cells 12 (11), 1487 (2023)
   PUBMED   37296607
  REMARK    GeneRIF: Phosphorylation of PACSIN2 at S313 Regulates Podocyte
            Architecture in Coordination with N-WASP.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4355)
  AUTHORS   Houy,S., Nicolas,G., Momboisse,F., Malacombe,M., Bader,M.F.,
            Vitale,N., Lecomte,M.C., Ory,S. and Gasman,S.
  TITLE     alphaII-spectrin controls calcium-regulated exocytosis in
            neuroendocrine chromaffin cells through neuronal Wiskott-Aldrich
            Syndrome protein interaction
  JOURNAL   IUBMB Life 72 (4), 544-552 (2020)
   PUBMED   31859439
  REMARK    GeneRIF: alphaII-spectrin controls calcium-regulated exocytosis in
            neuroendocrine chromaffin cells through neuronal Wiskott-Aldrich
            Syndrome protein interaction.
REFERENCE   3  (bases 1 to 4355)
  AUTHORS   Okrut,J., Prakash,S., Wu,Q., Kelly,M.J. and Taunton,J.
  TITLE     Allosteric N-WASP activation by an inter-SH3 domain linker in Nck
  JOURNAL   Proc Natl Acad Sci U S A 112 (47), E6436-E6445 (2015)
   PUBMED   26554011
  REMARK    GeneRIF: Nck was the most potent activator of N-WASP-driven actin
            assembly.
REFERENCE   4  (bases 1 to 4355)
  AUTHORS   Jaudon,F., Raynaud,F., Wehrle,R., Bellanger,J.M., Doulazmi,M.,
            Vodjdani,G., Gasman,S., Fagni,L., Dusart,I., Debant,A. and
            Schmidt,S.
  TITLE     The RhoGEF DOCK10 is essential for dendritic spine morphogenesis
  JOURNAL   Mol Biol Cell 26 (11), 2112-2127 (2015)
   PUBMED   25851601
REFERENCE   5  (bases 1 to 4355)
  AUTHORS   Banjade,S. and Rosen,M.K.
  TITLE     Phase transitions of multivalent proteins can promote clustering of
            membrane receptors
  JOURNAL   Elife 3, e04123 (2014)
   PUBMED   25321392
  REMARK    GeneRIF: biochemical reconstitution on supported lipid bilayers of
            protein clusters containing the adhesion receptor Nephrin and its
            cytoplasmic partners, Nck and N-WASP, is reported.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4355)
  AUTHORS   Qualmann,B. and Kelly,R.B.
  TITLE     Syndapin isoforms participate in receptor-mediated endocytosis and
            actin organization
  JOURNAL   J Cell Biol 148 (5), 1047-1062 (2000)
   PUBMED   10704453
REFERENCE   7  (bases 1 to 4355)
  AUTHORS   Qualmann,B., Roos,J., DiGregorio,P.J. and Kelly,R.B.
  TITLE     Syndapin I, a synaptic dynamin-binding protein that associates with
            the neural Wiskott-Aldrich syndrome protein
  JOURNAL   Mol Biol Cell 10 (2), 501-513 (1999)
   PUBMED   9950691
REFERENCE   8  (bases 1 to 4355)
  AUTHORS   Suetsugu,S., Miki,H. and Takenawa,T.
  TITLE     The essential role of profilin in the assembly of actin for
            microspike formation
  JOURNAL   EMBO J 17 (22), 6516-6526 (1998)
   PUBMED   9822597
REFERENCE   9  (bases 1 to 4355)
  AUTHORS   Miki,H., Sasaki,T., Takai,Y. and Takenawa,T.
  TITLE     Induction of filopodium formation by a WASP-related
            actin-depolymerizing protein N-WASP
  JOURNAL   Nature 391 (6662), 93-96 (1998)
   PUBMED   9422512
REFERENCE   10 (bases 1 to 4355)
  AUTHORS   Fukuoka,M., Miki,H. and Takenawa,T.
  TITLE     Identification of N-WASP homologs in human and rat brain
  JOURNAL   Gene 196 (1-2), 43-48 (1997)
   PUBMED   9322739
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from CH473959.1.
            
            On Nov 16, 2007 this sequence version replaced XM_001061794.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMD00132261, SAMD00132262
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..4355
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="4"
                     /map="4q22"
     gene            1..4355
                     /gene="Wasl"
                     /gene_synonym="N-WASP; TRS4"
                     /note="WASP like actin nucleation promoting factor"
                     /db_xref="GeneID:682507"
                     /db_xref="RGD:735144"
     exon            1..473
                     /gene="Wasl"
                     /gene_synonym="N-WASP; TRS4"
                     /inference="alignment:Splign:2.1.0"
     CDS             366..1871
                     /gene="Wasl"
                     /gene_synonym="N-WASP; TRS4"
                     /note="neural Wiskott-Aldrich syndrome protein;
                     Wiskott-Aldrich syndrome-like"
                     /codon_start=1
                     /product="actin nucleation-promoting factor WASL"
                     /protein_id="NP_001103835.1"
                     /db_xref="GeneID:682507"
                     /db_xref="RGD:735144"
                     /translation="MSSGQQPPRRVTNVGSLLLTPQENESLFSFLGKKCVTMSSAVVQ
                     LYAADRNCMWSKKCSGVACLVKDNPQRSYFLRIFDIKDGKLLWEQELYNNFVYNSPRG
                     YFHTFAGDTCQVALNFANEEEAKKFRKAVTDLLGRRQRKSEKRRDAPNGPNLPMATVD
                     IKNPEITTNRFYGSQVNNISHTKEKKKGKAKKKRLTKADIGTPSNFQHIGHVGWDPNT
                     GFDLNNLDPELKNLFDMCGISEAQLKDRETSKVIYDFIEKTGGVEAVKNELRRQAPPP
                     PPPSRGGPPPPPPPPHSSGPPPPPARGRGAPPPPPSRAPTAAPPPPPPSRPGVVVPPP
                     PPNRMYPPPPPALPSSAPSGPPPPPPLSMAGSTAPPPPPPPPPPPGPPPPPGLPSDGD
                     HQVPASSGNKAALLDQIREGAQLKKVEQNSRPVSCSGRDALLDQIRQGIQLKSVSDGQ
                     ESTPPTPAPTSGIVGALMEVMQKRSKAIHSSDEDEDDDDEEDFEDDDEWED"
     exon            474..608
                     /gene="Wasl"
                     /gene_synonym="N-WASP; TRS4"
                     /inference="alignment:Splign:2.1.0"
     exon            609..695
                     /gene="Wasl"
                     /gene_synonym="N-WASP; TRS4"
                     /inference="alignment:Splign:2.1.0"
     exon            696..792
                     /gene="Wasl"
                     /gene_synonym="N-WASP; TRS4"
                     /inference="alignment:Splign:2.1.0"
     exon            793..816
                     /gene="Wasl"
                     /gene_synonym="N-WASP; TRS4"
                     /inference="alignment:Splign:2.1.0"
     exon            817..985
                     /gene="Wasl"
                     /gene_synonym="N-WASP; TRS4"
                     /inference="alignment:Splign:2.1.0"
     exon            986..1028
                     /gene="Wasl"
                     /gene_synonym="N-WASP; TRS4"
                     /inference="alignment:Splign:2.1.0"
     exon            1029..1182
                     /gene="Wasl"
                     /gene_synonym="N-WASP; TRS4"
                     /inference="alignment:Splign:2.1.0"
     exon            1183..1700
                     /gene="Wasl"
                     /gene_synonym="N-WASP; TRS4"
                     /inference="alignment:Splign:2.1.0"
     exon            1701..1809
                     /gene="Wasl"
                     /gene_synonym="N-WASP; TRS4"
                     /inference="alignment:Splign:2.1.0"
     exon            1810..4355
                     /gene="Wasl"
                     /gene_synonym="N-WASP; TRS4"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ccgcgtcccg tggtctcgcc cgcccctgct gccatgttgg attgtgcggc tactgccgcc
       61 gccgccgccg ccgctgccgc cgccagagga ctggaggagt aaggagttta gcgcagccgc
      121 tggagtgcga ggacaacgac catccggccc gagccaagta gggcgggagt ccggagcttc
      181 cctgtcggcg cgcggcctga aggcgcccgc cgtcagctcc ttccctcggc ctccgcgcgc
      241 cggaggagcg acgggatgct gcgtctgtaa ctgccggctc gcgcccctgt cacccggagt
      301 gggaccgagt gctcgcccac caccagaaga gacggccctg gacactccac cccaccggcg
      361 acaccatgag ctcgggccag cagcccccgc ggagggtcac caacgtgggc tccctgctgc
      421 tcaccccgca agaaaacgag tctcttttct ccttcctcgg caagaaatgt gtgactatgt
      481 cttcagcagt ggtgcagtta tatgcagctg atcggaactg tatgtggtca aagaagtgca
      541 gtggtgttgc ttgtcttgtt aaggacaatc ctcagagatc ttatttttta agaatatttg
      601 acattaagga tgggaaatta ctgtgggaac aagagctata caataacttt gtatataata
      661 gtcctagagg atattttcat acctttgctg gagatacttg tcaagtagct cttaattttg
      721 ccaatgaaga agaagcaaaa aagttccgaa aagcagttac agacctgttg ggtcgacgac
      781 aaaggaaatc tgaaaaaaga cgagatgctc caaatggtcc caatctaccc atggctacag
      841 ttgacataaa aaatccagaa atcacaacaa acaggtttta tggttcacaa gtcaacaaca
      901 tctcccacac caaagaaaag aagaaaggaa aagctaaaaa gaagagatta accaaggcag
      961 atattggaac accaagtaat ttccagcaca ttggacatgt tggttgggat ccaaatacag
     1021 gttttgatct aaataatttg gatccagaat tgaagaatct ttttgatatg tgtgggatct
     1081 ctgaggccca gcttaaagac agagaaacat caaaagttat ttatgacttt attgaaaaaa
     1141 caggaggtgt agaagctgtt aaaaatgaac tccgaaggca agcaccacca cctcctccac
     1201 cctcaagagg aggacctccc cctcctcctc cccctcctca cagctcaggc cctcctcccc
     1261 ctcctgcccg tggaaggggg gctcctcccc cgccaccatc aagagctcct actgctgcac
     1321 ctccacctcc acctccttct aggcctggtg ttgtcgttcc tccacctcct ccaaacagga
     1381 tgtaccctcc tccaccacca gccctgcctt cctcagcacc ttcaggccca ccaccacctc
     1441 cgcctctgtc tatggcaggg tccacagcac caccacctcc tccaccacct ccccctccac
     1501 cagggccacc acctccccct ggcctgcctt ctgatggtga ccatcaagtt ccagcttctt
     1561 caggaaacaa agcagctctt ttggatcaaa ttagagaggg tgctcagcta aaaaaagtgg
     1621 agcagaatag tcggcccgtg tcctgctcag gaagggatgc acttctagac cagatacgac
     1681 agggcattca gttgaaatcc gtgtctgatg gccaagagtc cacaccacca acccccgcgc
     1741 ccacttcagg aattgtgggt gcgctgatgg aagtgatgca gaaaaggagc aaagccattc
     1801 attcctcaga tgaagatgaa gatgatgatg atgaagaaga ttttgaggat gatgatgagt
     1861 gggaagactg atctatatat tatatatata tttttaaggt gaaatactaa acactacttc
     1921 ctgtctattg atctgtaaaa ttatcatgta aatgttctac caatttgctg tatatttttg
     1981 ttgccttttt tgcttttttt tttaaattaa tctgtgcaat acctcattta atctgtaaag
     2041 gtttgttata atcctatata aagctactcc ctgttgctgt gtgcaagttt acaacaggat
     2101 gctcacgtga tttgtgaata ttttcattct atcaacaagg gagtttaatt atgtgtgaga
     2161 ctgacacaaa ccttaatgta atttagttag aatgccagaa ggagaacact attttcatac
     2221 cctacttttt ctgtacctaa attttcttaa taaaatttag tatagcacta cattcttttt
     2281 caagtgatgg aaatttagtt tctttagccc cttcattttg aatatgatgc cacatatgaa
     2341 tgttgaagct gatacattgc acaataccct agacaccact acactgatac agtttttcaa
     2401 attctagtat tatgtgttgc ttaacatcac tacagagatg ctgctgaaga gagacggcag
     2461 cggcggcgca cacctccagt gcagtagtgc ctgccgttgg taagcagtgg catcgcatcc
     2521 acaggcagga accctgacga ggcctcactc atgtgcctgt ccttgaccaa ggtgattttt
     2581 atgctgtggc aaatgtgaca ggctcttttt agcagagttt aacactcgtt atcacacccg
     2641 acatagtttc tggtagcatt tttcaaggta gtttcgtgca tctaaacaac tgtcatgccc
     2701 atatttgact cagaggggtg ttggaacagt gctgcaatgg aatgcagtta tctttgtatt
     2761 ttgcactttg tgggcatcgc tgtattcaaa gtaccaaccg ccagagtcgg agtttttggc
     2821 agaaatatgc acgaatgcta acccttgcgt ataccatata tttttatttt gatggccgct
     2881 ccaagttttt gtcaagtacc acgtgtatgt agagtttaca tttttcttaa aagattgatc
     2941 gtcccaatgc aaatttctta gaatacataa ctgatttgat agtttttttt aaataaaact
     3001 ttacagccgg cacagttgta cgcatactat atggtatgtt aaaattaggg tttccttgcc
     3061 actttacagt acactaatct gcctaaaggt ttcatattta cagtgataat tatcttacct
     3121 acctacttta aataaatttt aggtagatga ttacctgatt taagtgcaga catttttctc
     3181 acattgagtt gaatgcagaa tgtagcttca aatgtattca ttatagtcac aatatccaac
     3241 taaaaattat gctatctaga aatataccga ccaaaatcta gtattgacat gatcttgaca
     3301 ggctggacct gcgagatatg gtttgaattt taaccagttt atcacataac ctctagagac
     3361 catgcatata attattgtca ggaataattt aaaaggttga gttgctgaaa gcagttaagt
     3421 ggtgcagtaa gatagttaag ttattcaaag aatgttcccc tcctcgcaag agtaattaaa
     3481 gcacatctga aagattctca atgctgtgtt tcctgtaaaa acagtgctct ctgctgctgg
     3541 gaactctttc tgcttactgt cttttcatgt ggcttgagct ctgtgagacc gtgtagatga
     3601 ggctggacgg ctactaccca cacagttagg ggtctatagg tcggaactac tttaaataaa
     3661 tagcatcgga cgttcatatt tctttttctt tttgatgtga agaaaaaaat taatgcctaa
     3721 aaatgtcttt gagataatgc caattccgat gacccttacc ttttggccaa gaagaagttt
     3781 gggatctttc cagctaattt gctgtttgat cagaagcttg tctagagagc ctctgagttc
     3841 tttgagttgc atatacattg gggggatatt atttgccaaa ggtaaaattg agtttattct
     3901 ttttcataca gaaaagggtt ttgcttttta ctttgtttaa agtctaaatt acaggagctg
     3961 tggggttcag tccaggcatg aagactaatc cactctgagt caagtgtcag ttgtacagct
     4021 ccgtccccct cgtcttgcac tatggatgcc actcttcctg tgcgtcatct ggaagcctca
     4081 tggatatatc cagctctgtg cttgtctggt agcctttgcg tttaacaaac tagcatgagg
     4141 ccttttatat cttaaatgct atgtgataga caatttcagc tagccacata ttgtattatg
     4201 aaattcataa agattttcaa tcatccaatt ccatttatca actgaaattt tgtttagtat
     4261 gtgaattttt ttgaaatgta tagtaaataa gatgttgcac tggtggcaat tcatcatgga
     4321 acataataaa attaatttga ctcaaatagt ataaa
//
