LOCUS       NM_001398866            4161 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus WASH complex subunit 5 (Washc5), mRNA.
ACCESSION   NM_001398866 XM_002729816
VERSION     NM_001398866.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 4161)
  AUTHORS   Song,L., Rijal,R., Karow,M., Stumpf,M., Hahn,O., Park,L.,
            Insall,R., Schroder,R., Hofmann,A., Clemen,C.S. and Eichinger,L.
  TITLE     Expression of N471D strumpellin leads to defects in the
            endolysosomal system
  JOURNAL   Dis Model Mech 11 (9) (2018)
   PUBMED   30061306
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4161)
  AUTHORS   Tyrrell,B.J., Woodham,E.F., Spence,H.J., Strathdee,D., Insall,R.H.
            and Machesky,L.M.
  TITLE     Loss of strumpellin in the melanocytic lineage impairs the WASH
            Complex but does not affect coat colour
  JOURNAL   Pigment Cell Melanoma Res 29 (5), 559-571 (2016)
   PUBMED   27390154
REFERENCE   3  (bases 1 to 4161)
  AUTHORS   Jahic,A., Khundadze,M., Jaenisch,N., Schule,R., Klimpe,S.,
            Klebe,S., Frahm,C., Kassubek,J., Stevanin,G., Schols,L., Brice,A.,
            Hubner,C.A. and Beetz,C.
  TITLE     The spectrum of KIAA0196 variants, and characterization of a murine
            knockout: implications for the mutational mechanism in hereditary
            spastic paraplegia type SPG8
  JOURNAL   Orphanet J Rare Dis 10, 147 (2015)
   PUBMED   26572744
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4161)
  AUTHORS   Zhao,J. and Hedera,P.
  TITLE     Strumpellin and Spartin, Hereditary Spastic Paraplegia Proteins,
            are Binding Partners
  JOURNAL   J Exp Neurosci 9, 15-25 (2015)
   PUBMED   25987849
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4161)
  AUTHORS   Gallon,M. and Cullen,P.J.
  TITLE     Retromer and sorting nexins in endosomal sorting
  JOURNAL   Biochem Soc Trans 43 (1), 33-47 (2015)
   PUBMED   25619244
  REMARK    Review article
REFERENCE   6  (bases 1 to 4161)
  AUTHORS   Wang,F., Zhang,L., Zhang,G.L., Wang,Z.B., Cui,X.S., Kim,N.H. and
            Sun,S.C.
  TITLE     WASH complex regulates Arp2/3 complex for actin-based polar body
            extrusion in mouse oocytes
  JOURNAL   Sci Rep 4, 5596 (2014)
   PUBMED   24998208
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 4161)
  AUTHORS   Ryder,P.V., Vistein,R., Gokhale,A., Seaman,M.N., Puthenveedu,M.A.
            and Faundez,V.
  TITLE     The WASH complex, an endosomal Arp2/3 activator, interacts with the
            Hermansky-Pudlak syndrome complex BLOC-1 and its cargo
            phosphatidylinositol-4-kinase type IIalpha
  JOURNAL   Mol Biol Cell 24 (14), 2269-2284 (2013)
   PUBMED   23676666
REFERENCE   8  (bases 1 to 4161)
  AUTHORS   Harbour,M.E., Breusegem,S.Y., Antrobus,R., Freeman,C., Reid,E. and
            Seaman,M.N.
  TITLE     The cargo-selective retromer complex is a recruiting hub for
            protein complexes that regulate endosomal tubule dynamics
  JOURNAL   J Cell Sci 123 (Pt 21), 3703-3717 (2010)
   PUBMED   20923837
REFERENCE   9  (bases 1 to 4161)
  AUTHORS   Derivery,E., Sousa,C., Gautier,J.J., Lombard,B., Loew,D. and
            Gautreau,A.
  TITLE     The Arp2/3 activator WASH controls the fission of endosomes through
            a large multiprotein complex
  JOURNAL   Dev Cell 17 (5), 712-723 (2009)
   PUBMED   19922875
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000007.1.
            
            On Dec 27, 2021 this sequence version replaced XM_002729816.6.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26360190.781490.1,
                                           SRR26643294.29336.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760389,
                                           SAMEA5760434 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-144               JAXUCZ010000007.1  92825389-92825532   c
            145-428             JAXUCZ010000007.1  92816319-92816602   c
            429-574             JAXUCZ010000007.1  92815736-92815881   c
            575-659             JAXUCZ010000007.1  92814929-92815013   c
            660-760             JAXUCZ010000007.1  92814068-92814168   c
            761-953             JAXUCZ010000007.1  92811579-92811771   c
            954-1106            JAXUCZ010000007.1  92808798-92808950   c
            1107-1220           JAXUCZ010000007.1  92806775-92806888   c
            1221-1392           JAXUCZ010000007.1  92804831-92805002   c
            1393-1520           JAXUCZ010000007.1  92798845-92798972   c
            1521-1650           JAXUCZ010000007.1  92795521-92795650   c
            1651-1763           JAXUCZ010000007.1  92794497-92794609   c
            1764-1930           JAXUCZ010000007.1  92792574-92792740   c
            1931-2006           JAXUCZ010000007.1  92792424-92792499   c
            2007-2117           JAXUCZ010000007.1  92791601-92791711   c
            2118-2258           JAXUCZ010000007.1  92790661-92790801   c
            2259-2339           JAXUCZ010000007.1  92789903-92789983   c
            2340-2441           JAXUCZ010000007.1  92789265-92789366   c
            2442-2621           JAXUCZ010000007.1  92788418-92788597   c
            2622-2746           JAXUCZ010000007.1  92787127-92787251   c
            2747-2909           JAXUCZ010000007.1  92784971-92785133   c
            2910-3012           JAXUCZ010000007.1  92784110-92784212   c
            3013-3092           JAXUCZ010000007.1  92783942-92784021   c
            3093-3196           JAXUCZ010000007.1  92782981-92783084   c
            3197-3326           JAXUCZ010000007.1  92782156-92782285   c
            3327-3423           JAXUCZ010000007.1  92780688-92780784   c
            3424-3577           JAXUCZ010000007.1  92778538-92778691   c
            3578-3665           JAXUCZ010000007.1  92777021-92777108   c
            3666-4161           JAXUCZ010000007.1  92773625-92774120   c
FEATURES             Location/Qualifiers
     source          1..4161
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="7"
                     /map="7q33"
     gene            1..4161
                     /gene="Washc5"
                     /gene_synonym="RGD1564420"
                     /note="WASH complex subunit 5"
                     /db_xref="GeneID:362919"
                     /db_xref="RGD:1564420"
     exon            1..144
                     /gene="Washc5"
                     /gene_synonym="RGD1564420"
                     /inference="alignment:Splign:2.1.0"
     exon            145..428
                     /gene="Washc5"
                     /gene_synonym="RGD1564420"
                     /inference="alignment:Splign:2.1.0"
     CDS             243..3722
                     /gene="Washc5"
                     /gene_synonym="RGD1564420"
                     /note="WASH complex subunit strumpellin"
                     /codon_start=1
                     /product="WASH complex subunit 5"
                     /protein_id="NP_001385795.1"
                     /db_xref="GeneID:362919"
                     /db_xref="RGD:1564420"
                     /translation="MLDFLAENNLCGQAILRIVSCGNAIIAEVLRLSEFIPAVFLLKD
                     RADQQRYGDIIFDFSYFKGPEFWESKLEARPELQDLDEEFRENNIEIVTRFYLAFQSV
                     HKYIVDLNRYLDDLNEGVYIQQTLETVLLNEDGKQLLCEALYLYGVMLLVIDQKIEGE
                     VRERMLVSYYRYSAARSSADSNLDDICKLLRSTGYSSQPGAKRPPSYPESYFQRVPIN
                     PTFISMAIGRLRSDDIYNQVSAYPLPEHRSTALANQAAMLYVILYFEPSILHTHQAKM
                     REIVDKYFPDNWVISIYMGITVNLADAWEPYKAAKTALNNTLDLPNVKEQASRYASVS
                     ERVRAQVQQFLKEGYLREEVLLDNIPRLLNCLRDCNVAIRWLMLHTADSACDPNNKRL
                     RQIKDQILADSRYNPKILFQLLLDTAQFEFILKEMFKQMLSEKQSKWEHYKKEGSERM
                     IELADVFSGVKPLTRVEKNENLQAWFREISKQILSLNYDDSTAAGRKTVQLIQALEEV
                     QEFHQLESNLQVCQFLADTRKFLHQMIRTINIKEEVLITMQIVGDLSFAWQLIDSFTS
                     IMQESIRVNPSMVTKLRATFLKLASALDLPLLRINQANSPDLLSVSQYYSGELVSYVR
                     KVLQIIPESMFTSLLKIIKLQTHDIIEVPTRLDKDKLRDYAQLGPRYEVAKLTHAISI
                     FTEGILMMKTTLVGIIKVDPKQLLEDGIRKELVKRVAFALHRGLIFNPRAKPSELMPK
                     LKELGATMDGFHRSFEYIQDYVNIYGLKIWQEEVSRIINYNVEQECNNFLRTKIQDWQ
                     SIYQSTHIPIPKFAPVDESITFIGRLCREILRITDPKVTCYIDQLNTWYDMKTRQEVT
                     SSRLFSEIQTTLGTFGLNGLDRLLCFMIVKELQNFLSMFQKIILRERTVQETLKTLMN
                     AVSPLRSIVANSSKVYLAAITKTQKIWSTYLEAIMKVGQMQILRRQIANELNSSCRFD
                     SRHLAAALDNLNKALLADIEAHYRDPSLPYPKEDNTLLYEITAYLEAAGIHNPLNKIY
                     ITTKRLPYFPIVNFLFLIAQLPKLQYNKNLGMVCRKPADPVDWPPLVLGLLTLLKQFH
                     SRYTEQFLALIGQFIRSTMEQCTSQKMPEMPADAVGALLFLEDYVRYTKLPRRVAEAH
                     VPNFIFDEFRTVL"
     exon            429..574
                     /gene="Washc5"
                     /gene_synonym="RGD1564420"
                     /inference="alignment:Splign:2.1.0"
     exon            575..659
                     /gene="Washc5"
                     /gene_synonym="RGD1564420"
                     /inference="alignment:Splign:2.1.0"
     exon            660..760
                     /gene="Washc5"
                     /gene_synonym="RGD1564420"
                     /inference="alignment:Splign:2.1.0"
     exon            761..953
                     /gene="Washc5"
                     /gene_synonym="RGD1564420"
                     /inference="alignment:Splign:2.1.0"
     exon            954..1106
                     /gene="Washc5"
                     /gene_synonym="RGD1564420"
                     /inference="alignment:Splign:2.1.0"
     exon            1107..1220
                     /gene="Washc5"
                     /gene_synonym="RGD1564420"
                     /inference="alignment:Splign:2.1.0"
     exon            1221..1392
                     /gene="Washc5"
                     /gene_synonym="RGD1564420"
                     /inference="alignment:Splign:2.1.0"
     exon            1393..1520
                     /gene="Washc5"
                     /gene_synonym="RGD1564420"
                     /inference="alignment:Splign:2.1.0"
     exon            1521..1650
                     /gene="Washc5"
                     /gene_synonym="RGD1564420"
                     /inference="alignment:Splign:2.1.0"
     exon            1651..1763
                     /gene="Washc5"
                     /gene_synonym="RGD1564420"
                     /inference="alignment:Splign:2.1.0"
     exon            1764..1930
                     /gene="Washc5"
                     /gene_synonym="RGD1564420"
                     /inference="alignment:Splign:2.1.0"
     exon            1931..2006
                     /gene="Washc5"
                     /gene_synonym="RGD1564420"
                     /inference="alignment:Splign:2.1.0"
     exon            2007..2117
                     /gene="Washc5"
                     /gene_synonym="RGD1564420"
                     /inference="alignment:Splign:2.1.0"
     exon            2118..2258
                     /gene="Washc5"
                     /gene_synonym="RGD1564420"
                     /inference="alignment:Splign:2.1.0"
     exon            2259..2339
                     /gene="Washc5"
                     /gene_synonym="RGD1564420"
                     /inference="alignment:Splign:2.1.0"
     exon            2340..2441
                     /gene="Washc5"
                     /gene_synonym="RGD1564420"
                     /inference="alignment:Splign:2.1.0"
     exon            2442..2621
                     /gene="Washc5"
                     /gene_synonym="RGD1564420"
                     /inference="alignment:Splign:2.1.0"
     exon            2622..2746
                     /gene="Washc5"
                     /gene_synonym="RGD1564420"
                     /inference="alignment:Splign:2.1.0"
     exon            2747..2909
                     /gene="Washc5"
                     /gene_synonym="RGD1564420"
                     /inference="alignment:Splign:2.1.0"
     exon            2910..3012
                     /gene="Washc5"
                     /gene_synonym="RGD1564420"
                     /inference="alignment:Splign:2.1.0"
     exon            3013..3092
                     /gene="Washc5"
                     /gene_synonym="RGD1564420"
                     /inference="alignment:Splign:2.1.0"
     exon            3093..3196
                     /gene="Washc5"
                     /gene_synonym="RGD1564420"
                     /inference="alignment:Splign:2.1.0"
     exon            3197..3326
                     /gene="Washc5"
                     /gene_synonym="RGD1564420"
                     /inference="alignment:Splign:2.1.0"
     exon            3327..3423
                     /gene="Washc5"
                     /gene_synonym="RGD1564420"
                     /inference="alignment:Splign:2.1.0"
     exon            3424..3577
                     /gene="Washc5"
                     /gene_synonym="RGD1564420"
                     /inference="alignment:Splign:2.1.0"
     exon            3578..3665
                     /gene="Washc5"
                     /gene_synonym="RGD1564420"
                     /inference="alignment:Splign:2.1.0"
     exon            3666..4161
                     /gene="Washc5"
                     /gene_synonym="RGD1564420"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggtcacgtga cgggctggga accaggacag aaaccagggt ccgggaagct gactctctgc
       61 aacccgggac tctaggagat ctctggagcg cgagaggggg acccgggtgt ccagtttacg
      121 cggccagcat cggaccgctg gcaggtcttt aatggagcaa ccatttgtcc tacagatctg
      181 gcctcacatc atgatccaca gaccccagct ctgaagtcag tcagccattc ccacccttca
      241 caatgttgga tttcctggct gagaataatc tctgtggcca ggcaatcctg aggattgttt
      301 cctgtggcaa cgccatcatt gccgaggtgc tgcggctctc tgagtttatt cctgctgtgt
      361 tcctgctgaa agaccgggct gaccagcaga gatatggaga catcatcttc gacttcagct
      421 attttaaggg tccagagttc tgggaaagca aactggaagc cagacctgag ctacaggatt
      481 tagatgagga atttcgggaa aacaacatag aaattgtgac cagattttac ttagcctttc
      541 aaagtgtgca taaatacatt gtagacttaa acagatacct cgatgatctc aatgaaggag
      601 tttatattca gcaaacctta gaaacggtgc ttctcaatga ggatgggaaa caacttctat
      661 gtgaagccct gtacttatac ggagttatgc tgcttgtcat cgaccagaag attgaaggag
      721 aagtcagaga gagaatgctg gtttcttact atcgatacag tgctgctcga tcctctgctg
      781 actcaaatct ggatgatatc tgtaagctac tgcgaagcac ggggtattcc agccagccag
      841 gagccaaaag gccacccagc taccctgaaa gctatttcca gagagtgcct atcaacccga
      901 ccttcattag catggccatc ggacgcctgc gatcggatga catctacaac caggtctcgg
      961 cctacccttt gccagagcac cgcagcactg ccctggcgaa tcaagccgcc atgctgtacg
     1021 tgattctcta ctttgagcct tccattcttc acacccacca agcaaaaatg agagagatcg
     1081 tggataagta ctttccagat aactgggtaa tcagcattta tatgggtatc accgttaatc
     1141 tagccgatgc gtgggaacct tacaaagctg caaaaactgc tttgaacaat acccttgacc
     1201 tcccaaatgt caaagagcag gcaagcagat acgctagtgt cagcgaaaga gttcgcgccc
     1261 aggtacagca gtttctgaaa gagggctact tgagggagga ggtgcttctg gacaatatcc
     1321 ccagactgct gaactgcctg agagactgca atgtggccat ccgctggctg atgcttcaca
     1381 cagccgactc agcctgtgac ccaaacaaca agcgccttcg tcaaatcaag gaccagattc
     1441 tggcagactc taggtacaat cccaagatcc tcttccagct gctgctggac actgcacaat
     1501 ttgagtttat actcaaagag atgttcaagc aaatgctttc agagaaacaa tccaagtggg
     1561 agcattacaa gaaggagggc tccgagcgga tgatagagct cgctgatgtc ttttcagggg
     1621 tgaagccact aacccgagtg gagaaaaatg aaaatcttca agcttggttc agagagatct
     1681 caaaacaaat actgtctcta aattatgatg attctactgc tgcaggcagg aaaactgtgc
     1741 aactaataca agctttggaa gaggttcagg agtttcacca gttggaatca aatctacaag
     1801 tttgccagtt tcttgctgac acccggaagt ttctccatca aatgatcaga actatcaaca
     1861 tcaaagagga agtcctgatc acaatgcaga ttgtggggga cctttctttt gcctggcagt
     1921 taattgacag tttcacatcc atcatgcaag aaagcataag ggtaaatcca tccatggtta
     1981 ccaaactcag agccacgttc ctaaagcttg cctctgccct tgaccttccc ctgctacgta
     2041 tcaatcaagc caacagccct gacctcctca gcgtgtctca gtattactca ggagagttgg
     2101 tgtcgtacgt gagaaaggtt ttgcagatca ttccagaaag catgttcaca tctctcctga
     2161 agatcataaa gcttcagacc catgacatca ttgaagtgcc tactcgcctg gacaaggaca
     2221 agctgcggga ctatgctcag ctcggcccac gatacgaggt cgccaaactt actcatgcca
     2281 tctccatctt taccgagggc atcttgatga tgaaaacaac cctggtgggc atcatcaagg
     2341 tggatcccaa gcagttgctg gaggacggaa taaggaaaga gctggttaag cgcgtggctt
     2401 tcgccctcca tcggggactg atattcaacc ctcgtgccaa gccaagtgag ttgatgccca
     2461 agctgaaaga gctgggggcc accatggatg gattccatcg ttcctttgag tatatacagg
     2521 actatgtcaa catttacggc ctgaagatct ggcaggagga agtgtctcgt attataaatt
     2581 ataatgtgga acaagagtgt aataacttct taagaacaaa gattcaagac tggcaaagta
     2641 tctaccagtc cactcatatt ccaatcccga agttcgcacc tgtggatgag tctataacgt
     2701 ttattggtcg actctgcaga gaaatcttgc ggatcacaga cccaaaagtg acatgttaca
     2761 tagaccagct gaacacttgg tatgacatga aaactcgtca agaagtgacc agcagtcgcc
     2821 tcttctcaga aatccagacc accttgggga cttttggtct gaatggattg gacaggcttc
     2881 tgtgcttcat gattgtaaaa gaactacaga atttcctcag tatgtttcag aaaatcatcc
     2941 tgagagagag aactgttcaa gaaactttaa aaacactcat gaatgctgtc agccctctaa
     3001 gaagcatagt agcaaattca agtaaagttt atcttgctgc catcacgaaa acacagaaga
     3061 tctggagcac ttacctggag gctataatga aggttggaca gatgcagatc ctcagacgac
     3121 agatcgccaa tgagctgaac tcttcctgtc gatttgattc cagacatctg gcagctgctc
     3181 tggacaatct caataaggct ctcctggccg acatcgaagc tcactatcgg gatccttcgc
     3241 ttccttaccc caaagaagat aacacactct tatatgaaat cacagcctat ctggaggcag
     3301 ctggcattca caacccactg aataagattt acataacaac gaagcgccta ccctacttcc
     3361 cgatcgtcaa ctttctgttt ttgattgcac agttgccaaa acttcagtat aacaaaaatc
     3421 taggcatggt ctgccggaaa cctgccgacc ctgtggactg gccaccactc gtcctgggtc
     3481 tgctcactct gctgaagcag ttccactccc ggtacacaga gcagttcctg gcactgattg
     3541 gccagtttat tcgctccaca atggagcagt gcacaagcca gaagatgcct gaaatgcctg
     3601 cagatgctgt gggtgccctt ctgttcctgg aggattatgt tcggtacacg aaactccccc
     3661 gaagggtggc tgaagcgcat gtgcctaact tcatttttga tgaattccga acagtcctgt
     3721 gacttccttc cctgcttcag tgacaggatg ttctgtgact aaaatgaact tggaaatgaa
     3781 gatacttgac tcctcatgca actttcattt ccctcctctg tgggaattat cagccactct
     3841 aagatggctc tcagctcatt taatggcatc atatggaatt tttatcatgt agaagcagaa
     3901 ctgttttgta tgtgcctctt ctaaatgctc agtgtaactg atggatgaat gcatttcatc
     3961 ttatgttcta aagaactact tgtgcaactc cagatcttca gtaaaacagc attgccagta
     4021 ctgaactgcc ctgcttgtga tgaaaaactg acaccccgta agaaaccctg tgacagtctg
     4081 gggaggggtt gggggtgctc catccactgt aggtctgtaa ggaccattga cagcccagca
     4141 gtaaactcaa ctcatgttct a
//
