LOCUS       NM_001399772            5597 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus WASH complex subunit 4 (Washc4), mRNA.
ACCESSION   NM_001399772 XM_235003
VERSION     NM_001399772.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 5597)
  AUTHORS   Tyrrell,B.J., Woodham,E.F., Spence,H.J., Strathdee,D., Insall,R.H.
            and Machesky,L.M.
  TITLE     Loss of strumpellin in the melanocytic lineage impairs the WASH
            Complex but does not affect coat colour
  JOURNAL   Pigment Cell Melanoma Res 29 (5), 559-571 (2016)
   PUBMED   27390154
REFERENCE   2  (bases 1 to 5597)
  AUTHORS   Gallon,M. and Cullen,P.J.
  TITLE     Retromer and sorting nexins in endosomal sorting
  JOURNAL   Biochem Soc Trans 43 (1), 33-47 (2015)
   PUBMED   25619244
  REMARK    Review article
REFERENCE   3  (bases 1 to 5597)
  AUTHORS   Ryder,P.V., Vistein,R., Gokhale,A., Seaman,M.N., Puthenveedu,M.A.
            and Faundez,V.
  TITLE     The WASH complex, an endosomal Arp2/3 activator, interacts with the
            Hermansky-Pudlak syndrome complex BLOC-1 and its cargo
            phosphatidylinositol-4-kinase type IIalpha
  JOURNAL   Mol Biol Cell 24 (14), 2269-2284 (2013)
   PUBMED   23676666
REFERENCE   4  (bases 1 to 5597)
  AUTHORS   Harbour,M.E., Breusegem,S.Y., Antrobus,R., Freeman,C., Reid,E. and
            Seaman,M.N.
  TITLE     The cargo-selective retromer complex is a recruiting hub for
            protein complexes that regulate endosomal tubule dynamics
  JOURNAL   J Cell Sci 123 (Pt 21), 3703-3717 (2010)
   PUBMED   20923837
REFERENCE   5  (bases 1 to 5597)
  AUTHORS   Derivery,E., Sousa,C., Gautier,J.J., Lombard,B., Loew,D. and
            Gautreau,A.
  TITLE     The Arp2/3 activator WASH controls the fission of endosomes through
            a large multiprotein complex
  JOURNAL   Dev Cell 17 (5), 712-723 (2009)
   PUBMED   19922875
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000007.1.
            
            On Jan 14, 2022 this sequence version replaced XM_235003.10.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643290.25157.1,
                                           SRR26643298.38458.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-131               JAXUCZ010000007.1  22127698-22127828   c
            132-271             JAXUCZ010000007.1  22124870-22125009   c
            272-325             JAXUCZ010000007.1  22122207-22122260   c
            326-391             JAXUCZ010000007.1  22122054-22122119   c
            392-437             JAXUCZ010000007.1  22121392-22121437   c
            438-505             JAXUCZ010000007.1  22120470-22120537   c
            506-588             JAXUCZ010000007.1  22116764-22116846   c
            589-631             JAXUCZ010000007.1  22115789-22115831   c
            632-735             JAXUCZ010000007.1  22115289-22115392   c
            736-856             JAXUCZ010000007.1  22114331-22114451   c
            857-980             JAXUCZ010000007.1  22112528-22112651   c
            981-1108            JAXUCZ010000007.1  22112306-22112433   c
            1109-1269           JAXUCZ010000007.1  22110921-22111081   c
            1270-1396           JAXUCZ010000007.1  22106566-22106692   c
            1397-1522           JAXUCZ010000007.1  22100671-22100796   c
            1523-1630           JAXUCZ010000007.1  22098246-22098353   c
            1631-1777           JAXUCZ010000007.1  22097301-22097447   c
            1778-1857           JAXUCZ010000007.1  22097099-22097178   c
            1858-1963           JAXUCZ010000007.1  22096451-22096556   c
            1964-2080           JAXUCZ010000007.1  22095796-22095912   c
            2081-2249           JAXUCZ010000007.1  22094393-22094561   c
            2250-2404           JAXUCZ010000007.1  22093749-22093903   c
            2405-2479           JAXUCZ010000007.1  22091629-22091703   c
            2480-2584           JAXUCZ010000007.1  22090969-22091073   c
            2585-2719           JAXUCZ010000007.1  22089955-22090089   c
            2720-2828           JAXUCZ010000007.1  22087971-22088079   c
            2829-2895           JAXUCZ010000007.1  22085548-22085614   c
            2896-2982           JAXUCZ010000007.1  22084696-22084782   c
            2983-3130           JAXUCZ010000007.1  22083161-22083308   c
            3131-3227           JAXUCZ010000007.1  22081092-22081188   c
            3228-3424           JAXUCZ010000007.1  22080410-22080606   c
            3425-3524           JAXUCZ010000007.1  22079790-22079889   c
            3525-5597           JAXUCZ010000007.1  22075546-22077618   c
FEATURES             Location/Qualifiers
     source          1..5597
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="7"
                     /map="7q13"
     gene            1..5597
                     /gene="Washc4"
                     /gene_synonym="RGD1309995"
                     /note="WASH complex subunit 4"
                     /db_xref="GeneID:314690"
                     /db_xref="RGD:1309995"
     exon            1..131
                     /gene="Washc4"
                     /gene_synonym="RGD1309995"
                     /inference="alignment:Splign:2.1.0"
     CDS             71..3592
                     /gene="Washc4"
                     /gene_synonym="RGD1309995"
                     /note="WASH complex subunit 7"
                     /codon_start=1
                     /product="WASH complex subunit 4"
                     /protein_id="NP_001386701.1"
                     /db_xref="GeneID:314690"
                     /db_xref="RGD:1309995"
                     /translation="MAVDTLSPDWDFDRVDDGSQKIHAEVQLKNYGKFLEEYTSQLRR
                     IEDALDDLVGDVWDFNLDPIALKLLPYEQSSLLELIKTENKVLNKVITVYAALCCEIK
                     KLKHEAETKFYNGLLFYGEGAADSSMAEGDCQIQMGRFVSFLQELSCFVTRCYEVVMS
                     VIHQLAALYISNKTGPKIIETTGVHFQTMYEHLGELLTVLLTLDEIIDNHVTLKDHWT
                     MYKRLLKSVHHNPSKFGIQEERLKPFEKFLLKLEGQLLDGMIFQACIEQQFDSLNGGI
                     SVSKNSTFAEEFAHSIRSIFANVEAKLGEPSEIDQRDKYVGICGLFVLHFQIFRTVDK
                     KFYKSLLDICKKVPAVTLTANIIWFPDNFLIHKMPAAAKLLDRKSLQAIKIHRDAFLQ
                     QKAQSLTKDVQSYYVFVSSWMMKMESMLSKEQRTDTFAEDLTNTCNVFIQGFLYAYSI
                     STIIKTTMNLYMSMQKPMTRTSVKALCRLIELLKAVEHMFHRRSMVVADSVSHITQHL
                     QHQALNSISVAKKRVISDKKYSEQRLDVLSALVLAENTLNGPSTKQRRLVVSLALSVG
                     TQMKTFKDEELFPLQVVMKKLDLISELRERVQTQCDCCFLYWHRAVFPIYLDDVYENA
                     VDAARLHYMFSALRDCVPAMMHSRHLESHELLLDCYDKEIMDILNERLLDKLCKETEK
                     DLRLSVHTHLKLDDRNPFKVGRKDLALFFSLNPIRFFNRFIDIRAYVTHYLDKTFYNL
                     TTVALHDWATYSEMRNLATQRYGLVMTEAHLPSQTLEQGLDVLEIMRNIHIFVSRYLY
                     NLNNQIFIERTSNNKHLNTINIRHIANSIRTHGTGIMNTTVNFTYQFLKKKFYIFSQF
                     MYDEHIKSRLIKDIRFFREIKDQNDHKYPFDRAEKFNRGIRKLGITPEGQSYLDQFRQ
                     LLSQIGNAMGYIRMIRSGGLHCSSNAIRFVPDLEDIVSFEELVKEEGLAEETLRAARH
                     LDSVLSDHTRNSAEGTEYFKMLVDVFAPEFRRPKNIHLRNFYIIVPPLTLNFVEHSIS
                     CKEKLNKKNKIGAAFTDDGFAMGVAYTLKLLDQYQEFDSLHWFQSVREKYIKEIRAVA
                     KQQSVQSTSQDEKLLQTMNLTQKRLEVYLQEFELLYFSLSSARIFFRADKTAAEENQE
                     KKEKEEETKMSNGDGSESTVSADPVVK"
     exon            132..271
                     /gene="Washc4"
                     /gene_synonym="RGD1309995"
                     /inference="alignment:Splign:2.1.0"
     exon            272..325
                     /gene="Washc4"
                     /gene_synonym="RGD1309995"
                     /inference="alignment:Splign:2.1.0"
     exon            326..391
                     /gene="Washc4"
                     /gene_synonym="RGD1309995"
                     /inference="alignment:Splign:2.1.0"
     exon            392..437
                     /gene="Washc4"
                     /gene_synonym="RGD1309995"
                     /inference="alignment:Splign:2.1.0"
     exon            438..505
                     /gene="Washc4"
                     /gene_synonym="RGD1309995"
                     /inference="alignment:Splign:2.1.0"
     exon            506..588
                     /gene="Washc4"
                     /gene_synonym="RGD1309995"
                     /inference="alignment:Splign:2.1.0"
     exon            589..631
                     /gene="Washc4"
                     /gene_synonym="RGD1309995"
                     /inference="alignment:Splign:2.1.0"
     exon            632..735
                     /gene="Washc4"
                     /gene_synonym="RGD1309995"
                     /inference="alignment:Splign:2.1.0"
     exon            736..856
                     /gene="Washc4"
                     /gene_synonym="RGD1309995"
                     /inference="alignment:Splign:2.1.0"
     exon            857..980
                     /gene="Washc4"
                     /gene_synonym="RGD1309995"
                     /inference="alignment:Splign:2.1.0"
     exon            981..1108
                     /gene="Washc4"
                     /gene_synonym="RGD1309995"
                     /inference="alignment:Splign:2.1.0"
     exon            1109..1269
                     /gene="Washc4"
                     /gene_synonym="RGD1309995"
                     /inference="alignment:Splign:2.1.0"
     exon            1270..1396
                     /gene="Washc4"
                     /gene_synonym="RGD1309995"
                     /inference="alignment:Splign:2.1.0"
     exon            1397..1522
                     /gene="Washc4"
                     /gene_synonym="RGD1309995"
                     /inference="alignment:Splign:2.1.0"
     exon            1523..1630
                     /gene="Washc4"
                     /gene_synonym="RGD1309995"
                     /inference="alignment:Splign:2.1.0"
     exon            1631..1777
                     /gene="Washc4"
                     /gene_synonym="RGD1309995"
                     /inference="alignment:Splign:2.1.0"
     exon            1778..1857
                     /gene="Washc4"
                     /gene_synonym="RGD1309995"
                     /inference="alignment:Splign:2.1.0"
     exon            1858..1963
                     /gene="Washc4"
                     /gene_synonym="RGD1309995"
                     /inference="alignment:Splign:2.1.0"
     exon            1964..2080
                     /gene="Washc4"
                     /gene_synonym="RGD1309995"
                     /inference="alignment:Splign:2.1.0"
     exon            2081..2249
                     /gene="Washc4"
                     /gene_synonym="RGD1309995"
                     /inference="alignment:Splign:2.1.0"
     exon            2250..2404
                     /gene="Washc4"
                     /gene_synonym="RGD1309995"
                     /inference="alignment:Splign:2.1.0"
     exon            2405..2479
                     /gene="Washc4"
                     /gene_synonym="RGD1309995"
                     /inference="alignment:Splign:2.1.0"
     exon            2480..2584
                     /gene="Washc4"
                     /gene_synonym="RGD1309995"
                     /inference="alignment:Splign:2.1.0"
     exon            2585..2719
                     /gene="Washc4"
                     /gene_synonym="RGD1309995"
                     /inference="alignment:Splign:2.1.0"
     exon            2720..2828
                     /gene="Washc4"
                     /gene_synonym="RGD1309995"
                     /inference="alignment:Splign:2.1.0"
     exon            2829..2895
                     /gene="Washc4"
                     /gene_synonym="RGD1309995"
                     /inference="alignment:Splign:2.1.0"
     exon            2896..2982
                     /gene="Washc4"
                     /gene_synonym="RGD1309995"
                     /inference="alignment:Splign:2.1.0"
     exon            2983..3130
                     /gene="Washc4"
                     /gene_synonym="RGD1309995"
                     /inference="alignment:Splign:2.1.0"
     exon            3131..3227
                     /gene="Washc4"
                     /gene_synonym="RGD1309995"
                     /inference="alignment:Splign:2.1.0"
     exon            3228..3424
                     /gene="Washc4"
                     /gene_synonym="RGD1309995"
                     /inference="alignment:Splign:2.1.0"
     exon            3425..3524
                     /gene="Washc4"
                     /gene_synonym="RGD1309995"
                     /inference="alignment:Splign:2.1.0"
     exon            3525..5597
                     /gene="Washc4"
                     /gene_synonym="RGD1309995"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 agcggctggg gtgaggcccg cgtcgcggcg gtgactgctc ggggctgtgt ctgtgggagg
       61 ttccgcggcg atggccgtgg acactctgtc acccgactgg gacttcgacc gcgtggacga
      121 cggctctcag aaaattcatg ctgaagtgca gcttaagaac tatgggaaat ttctggagga
      181 gtatacatct cagctgagaa gaattgagga cgccctggat gatttggttg gagatgtttg
      241 ggatttcaat cttgatccta tagcattaaa gcttcttcca tatgagcagt cttccctttt
      301 agaactcata aagactgaaa acaaggtcct caacaaagtc atcactgttt atgctgcact
      361 ctgttgtgag atcaagaaat taaaacatga ggctgaaact aagttttaca atggtctcct
      421 gttttatggt gaaggagctg cagattccag catggcggaa ggtgactgcc aaatccaaat
      481 ggggaggttc gtttccttct tacaggaact gtcctgcttc gtgacgaggt gctacgaggt
      541 ggtgatgagt gtcatccatc agctggccgc cctgtacatc agcaacaaga caggacccaa
      601 aattatagag acaactggag ttcactttca gactatgtat gaacacttgg gagaactgct
      661 aacagtttta cttaccctgg atgaaattat tgacaatcat gtcactctga aggaccactg
      721 gactatgtac aaaaggttgc tgaaatctgt ccatcacaac ccttcaaaat ttgggattca
      781 ggaagaaaga ttaaagcctt ttgagaagtt cttgctgaag ctggaagggc agttgcttga
      841 tggaatgata ttccaggctt gtattgaaca acagtttgat tctctcaacg gagggatatc
      901 tgtgtcgaaa aatagcactt ttgcagaaga gttcgcacat agcattcgct caatttttgc
      961 caacgtggaa gccaagctcg gcgaaccttc tgaaattgac cagagagaca agtatgttgg
     1021 catttgtggg ctctttgtac tgcactttca gatttttcga actgttgata aaaagtttta
     1081 taagtcttta ttggacattt gtaagaaggt gccagccgtc actttaactg ctaatattat
     1141 ttggtttcct gataattttc tgattcataa aatgccagca gctgccaaac tcctagacag
     1201 aaaaagtctt caagccatta aaatacacag ggatgccttc ctacagcaga aagctcagtc
     1261 gctcaccaaa gatgtgcagt cttactacgt ctttgtgagc tcgtggatga tgaagatgga
     1321 gtctatgtta tctaaagagc agaggacgga tacatttgcc gaagacctca ccaacacatg
     1381 taatgttttc atacagggct tcttatatgc atatagtatt agcaccatta taaagaccac
     1441 gatgaacctc tacatgtcca tgcagaagcc tatgaccaga acctccgtga aggcgctgtg
     1501 caggctcatt gagcttctca aggcggtaga gcatatgttc cacaggagaa gcatggttgt
     1561 tgcggactcg gtgtcacata taacacagca ccttcagcat caggctctta actctatctc
     1621 tgtagccaag aaaagagtaa tttcagacaa gaaatacagt gaacagcgtc ttgatgtgct
     1681 gtctgctctt gttttggctg agaacacctt aaatggacca agcaccaaac aacgacgact
     1741 tgttgtttct ttggcgttga gtgttggcac gcaaatgaaa acattcaaag atgaagaact
     1801 ctttcccctg caagttgtca tgaaaaaact cgatcttatc agtgaactga gggaacgagt
     1861 ccaaacacag tgtgactgtt gttttttata ctggcatcgc gctgtcttcc cgatttattt
     1921 agatgatgtc tatgagaatg ctgttgatgc agctagatta cattacatgt tcagtgcttt
     1981 acgagactgt gtacctgcca tgatgcattc aaggcactta gagtcccatg agctgcttct
     2041 ggattgctat gacaaggaaa tcatggacat tttaaatgag cgtctgctgg ataagctgtg
     2101 taaggagacg gagaaggact tgcggctctc cgtgcacact cacttgaagc tggatgaccg
     2161 gaaccccttc aaagtgggca gaaaggactt ggctctgttt ttctctctga acccgattcg
     2221 gtttttcaac cgcttcattg acattcgagc ttatgtgact cactatctag ataagacttt
     2281 ctacaatctc acaacagtag ctcttcatga ttgggccact tacagtgaaa tgcgaaattt
     2341 ggctacgcag cgttatgggt tggttatgac agaggcacat cttcctagcc agactctgga
     2401 gcagggactc gacgttctgg aaatcatgag aaacattcac atatttgtct ctcgatacct
     2461 ctataatctc aataatcaga tttttattga acggacaagc aacaataaac atttgaatac
     2521 tattaatatt cgacatattg ctaattcaat tcgaacacat gggacaggga tcatgaatac
     2581 aacagttaat tttacctacc agtttttgaa aaagaagttc tatatattta gccagtttat
     2641 gtatgatgaa catatcaaat ccagattgat taaagacatt cggtttttca gggagatcaa
     2701 ggaccaaaat gatcacaagt atccttttga tagggcagaa aagttcaatc gcggcataag
     2761 aaaacttgga atcactccag agggacagag ctacctcgac cagttcaggc agctcctcag
     2821 tcagatcggt aatgctatgg gctatatacg aatgataaga tctggtggtc ttcattgtag
     2881 cagcaatgcc attagattcg tgccggatct tgaagacatt gtgagtttcg aagagctcgt
     2941 gaaggaagaa gggcttgcag aagaaacact tagagcagcc aggcatttgg actcggtcct
     3001 cagtgatcat acacgaaatt ctgccgaagg cacagaatac ttcaaaatgc ttgtagatgt
     3061 ttttgctcca gaatttcgaa ggccaaagaa tatacatctc cgaaatttct atatcattgt
     3121 ccctcctctc actctgaact ttgtagaaca ttctataagt tgcaaagaga aattgaataa
     3181 aaaaaacaaa ataggagctg cgtttactga cgatggtttt gccatgggtg tggcttatac
     3241 cttgaagctt ttggaccagt accaggagtt tgattcactt cactggttcc agtctgttag
     3301 agaaaaatac ataaaggaga taagagcggt tgctaagcaa cagagcgtgc agtcaaccag
     3361 tcaagatgaa aagctgctgc aaaccatgaa ccttactcag aagcggctgg aggtctatct
     3421 acaggaattt gaactgctgt atttctcctt gagcagtgca agaattttct tcagagcaga
     3481 caagactgcg gctgaagaga accaagaaaa gaaagagaag gaagaagaaa ctaaaatgag
     3541 caatggagac ggatccgaga gcactgtgtc tgcggatcct gtcgtgaagt gatgggatgc
     3601 ggtcgtcaga catgtcgtgc tttccagaga ctgacatgga tgctatgacg tgcttgataa
     3661 ttgtcacttc ctgggccact cctgtggaaa ctgcagtgcc ctggagtcag gcccacttta
     3721 tttctgatgc tctagctttc acgggcggag aagcagacgg taagactgga tctttgtagt
     3781 gtaggctgaa gagcccctgt gaaactctgc cgtgaggctg gcggcgggca agtccagatc
     3841 cctaacccat cgcctcccac ttctctactc tgtgtgtttg taatttgtct atgtctttga
     3901 tctgtgttga tctcaagtca tgggaaagtc aatgcatatg cggtacacag ccagtaaact
     3961 cgaagtttaa aaagggctga gcctgaaaga catgaagcgt ccgtctgtat cagaccgtga
     4021 ggatgctgga gatgagcaga aagaacggcc ttcctgtggg gttgggttcc acatgactca
     4081 ctctcatgag ctgctgatgt ctttggtgag acatttataa tagtttacat tacttttgga
     4141 acatttatcc tccaacaatt gctttaaatt taagatttgc ttaatattca aagaaaactc
     4201 agattaagcc atagacggga tcccacttga agtttcactg ggtttgaagg cactctgtga
     4261 tactagggaa gaaaaatgaa ttttcctaca tccaaactca ggtttcttca ttacattaga
     4321 ttgaaatctt ggtggcagtt tagacagatt tttactttaa atcgagtata atctaagaca
     4381 taagattgaa caataaccac actatgacag gtgttcagta atttaccact gtgagaatga
     4441 aactagtcag tcgcttaccg atctgaaaga atgaacggtt ttgagtaaat ttagttccca
     4501 acaagctact agctttattt ttgtaaaggt gtgctagtta atcgttaact gggttaatca
     4561 tggccttttt tgaacgtata gtaagatgat gcctttggga tgaaaaggaa ggtttagaac
     4621 tttaaatgta gtgcagacat tcttttggaa atgcttgttt ttaattgcat ttaccctttt
     4681 ctttggatac atcttgcatg taatcctaac acctagaatt ctgacctcct tccttggttc
     4741 ccacctttgg agtggagata cggtgcccag gctggatgca gtgcttaggt cagggtaact
     4801 gtctcagccc aagctaaaca ctttgactgt taagtcagaa gaaacagaat agattggaaa
     4861 tggtttttat tatataaaca tggcttagag aattatgaac aaagggtgga ttaaaggcat
     4921 ttaatgttta taattgatac taactgtaga gatggcccta aagcatgctg cataattaat
     4981 aatttatatt ttcattatta taaatgttta tattaatgat actggatttg attatatcag
     5041 attagtcacc attttatagt accaatattt tctctttttc ttaaaaatgt tctttgttta
     5101 attattcccg aacacgagaa ttcacaaact gactccctga acacttaaaa ttgggaagtg
     5161 acaggtaatt gtgttacctt tagttaaaaa taatgtcact gtagtctgga taaattgcca
     5221 tgaactcgtg aatttgaagt ccaggctcta aggagagaag tatttaaatg gtgttaagct
     5281 gccgagcaac agggcttcag tgtgttcaca tctgagttac tgtacagtgg agaatggttt
     5341 tccttgctaa caatattttt tcagaaatat tgtaatttaa atattaacat gagtgtttta
     5401 atgtaaattc acagaaaact taatgatgtt caaattctca ggaattaaga cctaaccata
     5461 acattgtcag ttctgattaa attttgtaaa agacagcaag tttgttatct catttgagag
     5521 gggttctctg ttcccctggc tctaatagaa tacagtatgt ataataaagc attaataaat
     5581 atgatttcaa aactgtc
//
