LOCUS       NM_001127390            1885 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus WASH complex subunit 1 (Washc1), mRNA.
ACCESSION   NM_001127390 XM_001072651 XM_346083
VERSION     NM_001127390.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Gokhale,A., Hartwig,C., Freeman,A.H., Das,R., Zlatic,S.A.,
            Vistein,R., Burch,A., Carrot,G., Lewis,A.F., Nelms,S.,
            Dickman,D.K., Puthenveedu,M.A., Cox,D.N. and Faundez,V.
  TITLE     The Proteome of BLOC-1 Genetic Defects Identifies the Arp2/3 Actin
            Polymerization Complex to Function Downstream of the Schizophrenia
            Susceptibility Factor Dysbindin at the Synapse
  JOURNAL   J Neurosci 36 (49), 12393-12411 (2016)
   PUBMED   27927957
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Tyrrell,B.J., Woodham,E.F., Spence,H.J., Strathdee,D., Insall,R.H.
            and Machesky,L.M.
  TITLE     Loss of strumpellin in the melanocytic lineage impairs the WASH
            Complex but does not affect coat colour
  JOURNAL   Pigment Cell Melanoma Res 29 (5), 559-571 (2016)
   PUBMED   27390154
REFERENCE   3  (bases 1 to 1885)
  AUTHORS   Bartuzi,P., Billadeau,D.D., Favier,R., Rong,S., Dekker,D.,
            Fedoseienko,A., Fieten,H., Wijers,M., Levels,J.H., Huijkman,N.,
            Kloosterhuis,N., van der Molen,H., Brufau,G., Groen,A.K.,
            Elliott,A.M., Kuivenhoven,J.A., Plecko,B., Grangl,G.,
            McGaughran,J., Horton,J.D., Burstein,E., Hofker,M.H. and van de
            Sluis,B.
  TITLE     CCC- and WASH-mediated endosomal sorting of LDLR is required for
            normal clearance of circulating LDL
  JOURNAL   Nat Commun 7, 10961 (2016)
   PUBMED   26965651
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1885)
  AUTHORS   Gallon,M. and Cullen,P.J.
  TITLE     Retromer and sorting nexins in endosomal sorting
  JOURNAL   Biochem Soc Trans 43 (1), 33-47 (2015)
   PUBMED   25619244
  REMARK    Review article
REFERENCE   5  (bases 1 to 1885)
  AUTHORS   Wang,F., Zhang,L., Zhang,G.L., Wang,Z.B., Cui,X.S., Kim,N.H. and
            Sun,S.C.
  TITLE     WASH complex regulates Arp2/3 complex for actin-based polar body
            extrusion in mouse oocytes
  JOURNAL   Sci Rep 4, 5596 (2014)
   PUBMED   24998208
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1885)
  AUTHORS   Piotrowski,J.T., Gomez,T.S., Schoon,R.A., Mangalam,A.K. and
            Billadeau,D.D.
  TITLE     WASH knockout T cells demonstrate defective receptor trafficking,
            proliferation, and effector function
  JOURNAL   Mol Cell Biol 33 (5), 958-973 (2013)
   PUBMED   23275443
REFERENCE   7  (bases 1 to 1885)
  AUTHORS   Zech,T., Calaminus,S.D., Caswell,P., Spence,H.J., Carnell,M.,
            Insall,R.H., Norman,J. and Machesky,L.M.
  TITLE     The Arp2/3 activator WASH regulates alpha5beta1-integrin-mediated
            invasive migration
  JOURNAL   J Cell Sci 124 (Pt 22), 3753-3759 (2011)
   PUBMED   22114305
REFERENCE   8  (bases 1 to 1885)
  AUTHORS   Monfregola,J., Napolitano,G., D'Urso,M., Lappalainen,P. and
            Ursini,M.V.
  TITLE     Functional characterization of Wiskott-Aldrich syndrome protein and
            scar homolog (WASH), a bi-modular nucleation-promoting factor able
            to interact with biogenesis of lysosome-related organelle subunit 2
            (BLOS2) and gamma-tubulin
  JOURNAL   J Biol Chem 285 (22), 16951-16957 (2010)
   PUBMED   20308062
REFERENCE   9  (bases 1 to 1885)
  AUTHORS   Derivery,E., Sousa,C., Gautier,J.J., Lombard,B., Loew,D. and
            Gautreau,A.
  TITLE     The Arp2/3 activator WASH controls the fission of endosomes through
            a large multiprotein complex
  JOURNAL   Dev Cell 17 (5), 712-723 (2009)
   PUBMED   19922875
REFERENCE   10 (bases 1 to 1885)
  AUTHORS   Gomez,T.S. and Billadeau,D.D.
  TITLE     A FAM21-containing WASH complex regulates retromer-dependent
            sorting
  JOURNAL   Dev Cell 17 (5), 699-711 (2009)
   PUBMED   19922874
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC166761.1.
            
            On or before May 17, 2008 this sequence version replaced
            XM_346083.3, XM_001072651.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC166761.1, SRR8487227.80109.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132262
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="9"
                     /map="9q37"
     gene            1..1885
                     /gene="Washc1"
                     /gene_synonym="ORF19; Wash; Wash1; Wash2"
                     /note="WASH complex subunit 1"
                     /db_xref="GeneID:367328"
                     /db_xref="RGD:1310196"
     exon            1..76
                     /gene="Washc1"
                     /gene_synonym="ORF19; Wash; Wash1; Wash2"
                     /inference="alignment:Splign:2.1.0"
     exon            77..230
                     /gene="Washc1"
                     /gene_synonym="ORF19; Wash; Wash1; Wash2"
                     /inference="alignment:Splign:2.1.0"
     CDS             82..1509
                     /gene="Washc1"
                     /gene_synonym="ORF19; Wash; Wash1; Wash2"
                     /note="WAS protein family homolog 2; WAS protein family
                     homolog 1"
                     /codon_start=1
                     /product="WASH complex subunit 1"
                     /protein_id="NP_001120862.1"
                     /db_xref="GeneID:367328"
                     /db_xref="RGD:1310196"
                     /translation="MTAVKTQHSLAGQVYAVPLIQPDLRREEAIQQVADALQYLQNIS
                     GDIFSRISQRVELSRRQLQAIGERVSLAQAKIEKIKGSKKAIKVFSSAKYPAPEHLQE
                     YNSVFTGALDPGLQRRPRYRIQSKHRPLDERALQEKLKYFPVCVSTKSEPEDEAEEGL
                     GGLPSNISSISSLLLFNTTENLYKKYVFLDPLAGAVTKTHTMLGTEEEKLFDAPLSIS
                     KREQLEQPAPENYFYVPGLGQVPEIDVPSYLPDLPGVADDLMYSADLGPGIAPSAPGA
                     IPELPAFHTEVAEPFQPEREDGALLAPPPPPPPPPPPPPPAPTAVVSAPQPPMSPDVV
                     TVTGKVAREEDSGSGEAHSASVQGAPKEVVDPSSGRATLLESIRQAGGIGKAKLRSVK
                     ERKLEKKKQKEQEQVRATSQGGDLMSDLFNKLVMRRKGISGKGPGTGTSEGPGGAFSR
                     MSDSIPPLPPPQQPAGDEDEDDWES"
     exon            231..342
                     /gene="Washc1"
                     /gene_synonym="ORF19; Wash; Wash1; Wash2"
                     /inference="alignment:Splign:2.1.0"
     exon            343..489
                     /gene="Washc1"
                     /gene_synonym="ORF19; Wash; Wash1; Wash2"
                     /inference="alignment:Splign:2.1.0"
     exon            490..626
                     /gene="Washc1"
                     /gene_synonym="ORF19; Wash; Wash1; Wash2"
                     /inference="alignment:Splign:2.1.0"
     exon            627..759
                     /gene="Washc1"
                     /gene_synonym="ORF19; Wash; Wash1; Wash2"
                     /inference="alignment:Splign:2.1.0"
     exon            760..961
                     /gene="Washc1"
                     /gene_synonym="ORF19; Wash; Wash1; Wash2"
                     /inference="alignment:Splign:2.1.0"
     exon            962..1141
                     /gene="Washc1"
                     /gene_synonym="ORF19; Wash; Wash1; Wash2"
                     /inference="alignment:Splign:2.1.0"
     exon            1142..1300
                     /gene="Washc1"
                     /gene_synonym="ORF19; Wash; Wash1; Wash2"
                     /inference="alignment:Splign:2.1.0"
     exon            1301..1369
                     /gene="Washc1"
                     /gene_synonym="ORF19; Wash; Wash1; Wash2"
                     /inference="alignment:Splign:2.1.0"
     exon            1370..1855
                     /gene="Washc1"
                     /gene_synonym="ORF19; Wash; Wash1; Wash2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gttcggggca gggaaggggc gaactgcgag cgcgggaccg aggggaggcc gcgaccctgc
       61 tgcccaccgg ctggaggcac catgacggcg gtgaagaccc agcactcctt ggcaggccag
      121 gtttatgcgg tgcccctcat ccagccagac ctgcgtcgag aggaggcaat ccagcaagtg
      181 gcagacgccc tgcagtactt acagaacatc tccggagaca tcttcagcag gatctcccag
      241 cgagtagagc tgagccgacg ccagctgcag gccattgggg agagggtctc cttggcccag
      301 gctaagatag agaagatcaa gggcagtaag aaggccatca aggtcttttc cagtgccaag
      361 tacccagccc cggagcacct gcaggagtat aactccgtct ttactggagc cctggaccct
      421 ggtctgcaga gaagaccccg gtacaggatc cagagcaagc accgcccact ggatgagcgg
      481 gctctgcagg agaagctgaa atactttccc gtgtgtgtga gcaccaagtc ggagcctgag
      541 gatgaggctg aggagggact tgggggtctt cccagcaaca ttagctccat cagctccctg
      601 ctgctcttca acaccacaga aaacctgtat aagaagtatg ttttcctgga ccctctggct
      661 ggtgcagtaa cgaaaacaca caccatgctg ggcacagagg aggagaagtt gttcgatgct
      721 cctctgtcca tcagcaagag agagcagctg gagcagccag ctccagaaaa ctacttctat
      781 gtgccaggcc tgggccaggt tcctgagatc gatgtgccat cctacctacc cgacttgcct
      841 ggcgtcgcag acgacctgat gtacagtgcg gatttgggtc ccggtattgc cccgtccgct
      901 cctggtgcta ttccagagct gcctgccttc catacggagg tggcggaacc tttccagcca
      961 gaacgggagg atggagcgct gttggcacca ccaccgcctc ctcctcctcc tccaccgcca
     1021 cctcccccag ctcccacagc ggtggtcagt gcgccccaac caccgatgtc ccctgatgtg
     1081 gtaaccgtga ctggcaaggt tgccagggaa gaggactctg gcagcggtga ggcccactca
     1141 gcctctgttc agggagcccc taaggaggtg gttgacccct ccagcggccg ggccactctg
     1201 cttgagtcca tccgccaagc cgggggcatt ggcaaggcca agctgcgcag tgtcaaggaa
     1261 cgcaagcttg agaagaagaa acagaaggag caggagcaag tgagagctac cagccaaggc
     1321 ggggacttga tgtcagatct cttcaacaaa ctcgtcatga ggcgcaaagg tatctctgga
     1381 aaaggacctg gcactggcac cagtgagggg cctggtggag ccttcagtcg aatgtcagac
     1441 tccattccac ctctgccgcc cccacagcag ccagcaggag acgaggacga ggatgactgg
     1501 gagtcctaag agtgctttgg cccttctccc ccaccagcag gaacagcctg caggggctcc
     1561 tgggccagtc ggtgcctgtc aggctctgtt accaaggagc tgcaggggtt tgtgcaggtg
     1621 tcccaagcct gtgcctaaat tctcttcatg gagtcgggct ctgtgagccg tctgctgcct
     1681 tccctgtttc atgcgactgc ttcactggat ccttccccga agtgcttctt cctttgaagg
     1741 atcctgagag aaaacagcaa catgctggac taaagaaagt aactgtaggc ctgaacccca
     1801 gccaacagtg gccttttaat aaataaagag cagccatgcg ggttgtggct tcggaaaaaa
     1861 aaaaaaaaaa aaaaaaaaaa aaaaa
//
