LOCUS       NM_001394051            5667 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus WASP family member 2 (Wasf2), transcript variant
            2, mRNA.
ACCESSION   NM_001394051 XM_039109780
VERSION     NM_001394051.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 5667)
  AUTHORS   Li,Y., Liu,S., Liu,H., Cui,Y. and Deng,Y.
  TITLE     Dragon's Blood Regulates Rac1-WAVE2-Arp2/3 Signaling Pathway to
            Protect Rat Intestinal Epithelial Barrier Dysfunction Induced by
            Simulated Microgravity
  JOURNAL   Int J Mol Sci 22 (5), 2722 (2021)
   PUBMED   33800361
  REMARK    GeneRIF: Dragon's Blood Regulates Rac1-WAVE2-Arp2/3 Signaling
            Pathway to Protect Rat Intestinal Epithelial Barrier Dysfunction
            Induced by Simulated Microgravity.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5667)
  AUTHORS   Bayes,A., Collins,M.O., Reig-Viader,R., Gou,G., Goulding,D.,
            Izquierdo,A., Choudhary,J.S., Emes,R.D. and Grant,S.G.
  TITLE     Evolution of complexity in the zebrafish synapse proteome
  JOURNAL   Nat Commun 8, 14613 (2017)
   PUBMED   28252024
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5667)
  AUTHORS   Burgers,P.P., van der Heyden,M.A., Kok,B., Heck,A.J. and
            Scholten,A.
  TITLE     A systematic evaluation of protein kinase A-A-kinase anchoring
            protein interaction motifs
  JOURNAL   Biochemistry 54 (1), 11-21 (2015)
   PUBMED   25097019
REFERENCE   4  (bases 1 to 5667)
  AUTHORS   Guo,Z., Neilson,L.J., Zhong,H., Murray,P.S., Zanivan,S. and
            Zaidel-Bar,R.
  TITLE     E-cadherin interactome complexity and robustness resolved by
            quantitative proteomics
  JOURNAL   Sci Signal 7 (354), rs7 (2014)
   PUBMED   25468996
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5667)
  AUTHORS   Murk,K., Blanco Suarez,E.M., Cockbill,L.M., Banks,P. and
            Hanley,J.G.
  TITLE     The antagonistic modulation of Arp2/3 activity by N-WASP, WAVE2 and
            PICK1 defines dynamic changes in astrocyte morphology
  JOURNAL   J Cell Sci 126 (Pt 17), 3873-3883 (2013)
   PUBMED   23843614
  REMARK    GeneRIF: The Arp2/3 regulators PICK1, and N-WASP and WAVE2 function
            antagonistically to control the complexity of astrocyte branched
            morphology, and this mechanism underlies the morphological changes
            seen in astrocytes during their response to pathological insult.
REFERENCE   6  (bases 1 to 5667)
  AUTHORS   Hirao,N., Sato,S., Gotoh,T., Maruoka,M., Suzuki,J., Matsuda,S.,
            Shishido,T. and Tani,K.
  TITLE     NESH (Abi-3) is present in the Abi/WAVE complex but does not
            promote c-Abl-mediated phosphorylation
  JOURNAL   FEBS Lett 580 (27), 6464-6470 (2006)
   PUBMED   17101133
REFERENCE   7  (bases 1 to 5667)
  AUTHORS   Choi,J., Ko,J., Racz,B., Burette,A., Lee,J.R., Kim,S., Na,M.,
            Lee,H.W., Kim,K., Weinberg,R.J. and Kim,E.
  TITLE     Regulation of dendritic spine morphogenesis by insulin receptor
            substrate 53, a downstream effector of Rac1 and Cdc42 small GTPases
  JOURNAL   J Neurosci 25 (4), 869-879 (2005)
   PUBMED   15673667
REFERENCE   8  (bases 1 to 5667)
  AUTHORS   Yamazaki,D., Suetsugu,S., Miki,H., Kataoka,Y., Nishikawa,S.,
            Fujiwara,T., Yoshida,N. and Takenawa,T.
  TITLE     WAVE2 is required for directed cell migration and cardiovascular
            development
  JOURNAL   Nature 424 (6947), 452-456 (2003)
   PUBMED   12879075
REFERENCE   9  (bases 1 to 5667)
  AUTHORS   Yan,C., Martinez-Quiles,N., Eden,S., Shibata,T., Takeshima,F.,
            Shinkura,R., Fujiwara,Y., Bronson,R., Snapper,S.B., Kirschner,M.W.,
            Geha,R., Rosen,F.S. and Alt,F.W.
  TITLE     WAVE2 deficiency reveals distinct roles in embryogenesis and
            Rac-mediated actin-based motility
  JOURNAL   EMBO J 22 (14), 3602-3612 (2003)
   PUBMED   12853475
REFERENCE   10 (bases 1 to 5667)
  AUTHORS   Sun,P., Yamamoto,H., Suetsugu,S., Miki,H., Takenawa,T. and Endo,T.
  TITLE     Small GTPase Rah/Rab34 is associated with membrane ruffles and
            macropinosomes and promotes macropinosome formation
  JOURNAL   J Biol Chem 278 (6), 4063-4071 (2003)
   PUBMED   12446704
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000005.1.
            
            On Mar 26, 2021 this sequence version replaced XM_039109780.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26360190.483587.1,
                                           SRR26643296.16298.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-193               JAXUCZ010000005.1  150620733-150620925
            194-366             JAXUCZ010000005.1  150662888-150663060
            367-501             JAXUCZ010000005.1  150671352-150671486
            502-655             JAXUCZ010000005.1  150672001-150672154
            656-773             JAXUCZ010000005.1  150674770-150674887
            774-904             JAXUCZ010000005.1  150675852-150675982
            905-1060            JAXUCZ010000005.1  150678170-150678325
            1061-1572           JAXUCZ010000005.1  150680472-150680983
            1573-5667           JAXUCZ010000005.1  150681849-150685943
FEATURES             Location/Qualifiers
     source          1..5667
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="5"
                     /map="5q36"
     gene            1..5667
                     /gene="Wasf2"
                     /note="WASP family member 2"
                     /db_xref="GeneID:313024"
                     /db_xref="RGD:1307299"
     exon            1..193
                     /gene="Wasf2"
                     /inference="alignment:Splign:2.1.0"
     exon            194..366
                     /gene="Wasf2"
                     /inference="alignment:Splign:2.1.0"
     CDS             237..1730
                     /gene="Wasf2"
                     /note="wiskott-Aldrich syndrome protein family member 2;
                     WAS protein family, member 2"
                     /codon_start=1
                     /product="actin-binding protein WASF2"
                     /protein_id="NP_001380980.1"
                     /db_xref="GeneID:313024"
                     /db_xref="RGD:1307299"
                     /translation="MPLVTRNIEPRHLCRQTLPSDTSELECRTNITLANVIRQLGSLS
                     KYAEDIFGEICTQASAFASRVNSLAERVDRVQVKVTQLDPKEEEVSLQGINTRKAFRS
                     STIQDQKLFDRNSLPVPVLETYNSCDAPPPLNNLSPYRDDGKEALKFYTNPSYFFDLW
                     KEKMLQDTKDIMKEKRKHRKEKKDNPNRGNVNPRKIKTRKEEWEKMKMGQEFVESKEK
                     LGPSGYSPALVYQNGNFGSVENVDATSYPPPPQSDSASSPSPSFSDDNLPPPPAEFSY
                     PADNQRGSVLAGPKRTSMVSPSHPPPAPPLSSPPGPKPGFAPPPAPPPPPPMSVPPPL
                     PSVGFGSPGTPPPPSPPSFPPHPDFAAPPPPPPPPAADYPMPPPPLSQPSGGAPPPPP
                     PPPPPGPPPLPLGGADGQPAAPPPPPVSDAAKPKSSLPAVSDARSDLLSAIRQGFQLR
                     RVEEQREQEKRDVVGNDVATILSRRIAVEYSDSEDDSSEFDEDDWSD"
     exon            367..501
                     /gene="Wasf2"
                     /inference="alignment:Splign:2.1.0"
     exon            502..655
                     /gene="Wasf2"
                     /inference="alignment:Splign:2.1.0"
     exon            656..773
                     /gene="Wasf2"
                     /inference="alignment:Splign:2.1.0"
     exon            774..904
                     /gene="Wasf2"
                     /inference="alignment:Splign:2.1.0"
     exon            905..1060
                     /gene="Wasf2"
                     /inference="alignment:Splign:2.1.0"
     exon            1061..1572
                     /gene="Wasf2"
                     /inference="alignment:Splign:2.1.0"
     exon            1573..5667
                     /gene="Wasf2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggctgcgtcc cggggcgggg aaatcgtgta atggcggact caggccggtg gagtgcggtt
       61 gggggcgtag cgcgctgagg ggggcgtccg gctgtgtagc agcccgcaaa gcggtgggct
      121 ggagcgtact ccgtggggac agggaccggc tgcccagccc ttcatgtcgt tgagggcgac
      181 tgcgcgaagt caggttgttt ttcatcactg agaaccttgc ctgaagcagg tccactatgc
      241 ccttagtaac caggaacatc gagccaaggc acctgtgccg tcagacgttg cctagcgata
      301 caagcgagct ggaatgcagg accaacatca ccctggcaaa tgtcatccga cagctgggca
      361 gcctgagtaa gtatgcggag gacattttcg gagagatttg tactcaggca agtgcctttg
      421 cctcccgagt gaactccctt gctgagcggg ttgaccgggt acaagttaaa gtcactcagc
      481 tggatcccaa ggaagaagaa gtgtcactgc aaggaatcaa cactcgtaag gccttcagaa
      541 gctctaccat ccaagaccag aagctttttg acagaaattc ccttccagta ccagtcttgg
      601 aaacctataa cagctgtgac gctcctcctc ctcttaacaa tctaagtcct tacagggacg
      661 acggaaaaga ggcgctcaaa ttctacacca acccttcgta cttctttgat ctttggaagg
      721 agaagatgct gcaggacacc aaggatatca tgaaagagaa gagaaagcac aggaaagaaa
      781 agaaagataa tccaaacaga gggaatgtga acccacgtaa aatcaagaca cgcaaggaag
      841 agtgggagaa gatgaaaatg ggacaagaat ttgtggagtc caaggagaag ctggggcctt
      901 ctggatactc gcccgccttg gtgtaccaga atggcaactt tggctctgtt gaaaacgtgg
      961 atgcgacaag ctacccacca ccgccacagt cggactctgc ctcctcacct tccccttcat
     1021 tctctgatga caacttgcct cccccaccag cagaattcag ctaccctgca gacaaccaaa
     1081 gaggatctgt cttggctgga cccaagagaa ccagcatggt cagcccaagc catccacccc
     1141 cagctcctcc tctgagctcc ccgccaggtc ccaaacctgg ctttgcccct ccacctgccc
     1201 ctccacctcc acctccaatg agcgtgccgc ctccactgcc atctgtagga ttcgggtctc
     1261 ctgggacccc tccaccaccc tcgcctccct ctttccctcc ccaccctgac tttgctgctc
     1321 ctccaccacc tccccctcca ccagcagctg actacccaat gccaccacct cccttgtccc
     1381 aaccatctgg aggcgctcct ccgcctcccc ctccccctcc ccctccaggg cccccacctc
     1441 ttcccttggg tggtgctgat ggccagcctg cagctccacc accacctcct gtttctgatg
     1501 ccgccaagcc caagtcttcg ttgcctgctg tgagtgatgc tcgtagtgac ctgctttctg
     1561 ccatccgcca aggctttcaa ctgcgaaggg ttgaagagca gcgggagcaa gagaagcgtg
     1621 atgtggtggg caatgatgtg gccaccatcc tgtcccggcg aattgctgtt gagtacagcg
     1681 actcagaaga cgactcctct gaatttgatg aagatgactg gtcggattaa ctgccttttg
     1741 cccacattcc tccttctgct ctccccaccc gccttctcag atacaaactg taacagtctc
     1801 atgtggggaa ggaagaaagg acaaagaact gcaaggggcc aaagccttct ctggaacaac
     1861 caaagatcta tccgagtgct tccttcaaac taccatacac ttcttccctg tccccagctg
     1921 tgggctcctg agggccgtgg ctgagctgat ccctctcccc ctccagtgac tgttctatgt
     1981 taacaggaag gaaaccccta cagcagatcc ctttggttgg gtttcagctg gagatagtgc
     2041 ctttccctga taaccattct cacccatggc cttcttgcat ctagctactt caaataatgc
     2101 tgtccacctc ctggtcttac agcagtgggc acagtctgtg tggacacagc tggtcccttt
     2161 tctgtgccct gaaactataa agcaggccaa gtgccctggc acgtggactg ccaccccctc
     2221 ccccacaccc cgccctctgc agcaggtagg gccgcttcac ttcagctcgg tctggacgcc
     2281 tttgttccag aaggcatcag gcccctaaga ctgtctcact cctctgccca gaagtgatat
     2341 cctgctgaag gctgaggctg cggccttcct cccatgctcc ctcttggtga gtgtggtgtt
     2401 tagatgtcct gtgcatgccc ctgctcaccc caggtgcctt aagactgcac agcccagttt
     2461 taacctctca tattcgtgac caaacgaccc tgacgcacag ggacctgggc ctctgccgac
     2521 cgtcaggagc aaaacttgaa ggtggggcac ggtaggactg gaagagaatg gagcaccttg
     2581 ggtcctgcct gctcaggatg ccgaggccag gatgagaagt gactctctca aggtcacaca
     2641 ggtagtggta gaacaagagc ccaggcttcc tgtttccaag tcacctgtgt ttcctgggac
     2701 caaatactga gcacctgctg gagtctgggc agagcagcct ggccctgttc tactccaggc
     2761 ctcccgaatg caggggttgc attggagggt tcacggcccg tgccaagctc agcagaacag
     2821 ttttaacact cggcctctct ggtaaccgtt tatttctcct tctgtcccca gccagtcgtg
     2881 tggcacggga gctgtgctgg gaagcagagt ggagaggagc tgtggggcag ccagccttgg
     2941 caggtgcctg tgcagttcac tgcagctccc tccccccttt gtttcctcga tttaagcata
     3001 gactgtgcca gctgcaggaa ggaacttcag tccctcaggc tggcagccac agcaggtacc
     3061 tgcctggggg agcctggcag ccatggagaa ggctgaaagg cagaaagaca caggtcttct
     3121 gcctctctac ctcactgtgc atggcgatgc tgaccccccc ttacctccct tcccagaact
     3181 aatacacggg tgctattagt caggaaaaaa actgagcagc tctggccagc agcaaggttt
     3241 cttctcttct gccccaacta ttgtgtggcc tcttgtgctg aaatcggctt ctctggcttc
     3301 agagcctgaa acaaagagaa acaggatctg tctctaccca gcacagcaaa tggtggtagt
     3361 aattgccaaa gccctcataa acccctccgg cttgaggaga gtgcaatcag gctctgccgc
     3421 ctgcacttgc tgagaacctc ccctctgcct cctttcctga actcagaggt agggactaag
     3481 cacaggtgcc agcaggccct cctgctgtgg ctactccaga gtgctctgtg tctccatgtg
     3541 tccgcatccc ccaccccgca tcccccaccc ccgtcccccc aaatccagtg tctggcacag
     3601 ggcttggcgc tcaactccat gctgtgcatg tttcccaccc tgtgccttag aagctgaagg
     3661 ctctttcatc agaaccttaa aatggctgtt gatggtaccc tggctgcagc ccagcagggg
     3721 cctggagagg ctggggaggg cagtggtcct tccaaataga agtcctgggg cctggccagg
     3781 ccagggtttg ggcctaatgg tttttactaa attactcccc tcctgctctc tgagaaaggg
     3841 gagccagagc cgttcactgt ggttctgttc cgaccttgaa ggggcggtat tggcctggct
     3901 tctggaatgg acagagtcca ccaggaaaga ctgggggcgg aaggagatgg ggaggggccc
     3961 tgtctgcgga aggtaggatt agatcattag ctcagtgacc tcctagggtt tctatgtgct
     4021 gtgttctcat cctacagctg gtttggtaat gatctgcaag tcccggagag caacagcaca
     4081 gctctgcctg acgctctcat taaaatccat gcagccaagc tctgcgcttt gtagcagtcg
     4141 gccttgcaga gcctcctcag cttggggggc tggggaccca gtcagctgag aggtccccta
     4201 ggctctactt aggcatatgt gtatccccca ccaaaaaaaa aatgtttttt aaaacatgct
     4261 tctttaaacc cagagccctt taaaataata aaggacccgg cgcaggtggg tgtgtttgtg
     4321 gaatcctgta aatggggaac acaaagttta gtgacattta aactgggctg aggctgtaag
     4381 tgtataaagc agtggcccat aaggactctt aagcgctgaa gccaaggagc cacctccctt
     4441 cccagtctgc ctagcccagg gggcctgccc ccattcctgt agcccagggg cccagcttgc
     4501 aattggtagt gtcagaagtg acttgtagct ttgccttggt tgtgagatga tttcattcca
     4561 ggggaggggg tggggggatc ccttggaggc tcagagcagc tggcccaagc tccggctcgc
     4621 ctccagtgga agctgcagcg ccctcagaaa cgaccctggc caatgtcact gcagagctga
     4681 ggcggccttg tcagggctaa tgtgaaatct cttcccgtgg cgccgatcct ctgactctat
     4741 agcgccagct tggacagatt gaggtctctg tggctttggt gaccctgctt aagcattccc
     4801 accgtgtccc tgctctgcac gaggactgcc tcctgctgca gccccttact tgacctcagg
     4861 gcctggggag ggaagcactg ggccaggacc aagcgctcca actcaggaga tatgtcagcc
     4921 tttgtgggga ggagggggtg aagcattgag cttggctggt gcccatccga cctgggacca
     4981 ggtgttgcac agagaagtgc ttctgtgcct cctgcctcgt tcactttagt ggggcctcag
     5041 cgaaggctga ggacaattct gttcctgtat taactttatt cagttaattg acctgaggaa
     5101 ctaaccaatt tttattttct gcctagaatg atttacacat aggagagaaa gctatcatgt
     5161 taccaaagtg ccttgttttc tctgatataa atatatttat aaggacatat tccagtaggt
     5221 tcttggtgtt tcccatatca agatttccac atagtgggaa taccaggccc ttttctccca
     5281 gaggttggaa ggggaaatct gagcagtgga atgaagccac cccacatggt gccccctctg
     5341 cccttgtcct acaggtaaaa gaagatacac acacacacaa aaattttttt gagatggtgt
     5401 ttcaggtaga tgacatgggc cttgacctta ctatgtagct gaagatggcc ttgaactcct
     5461 gaccctctgc ctcagccatg cctgactagg acatccgatt tttttttgtt ttaagtctgg
     5521 tttcatttgc ttcactgcca ggctcacagt gtgtaccatc gtatttgtac tcctacaacc
     5581 cgaagattcc atgtaactta tgtatgcgca tgaatacttt aaatgccaac attttcaata
     5641 atttgataaa gtctttgtag tcagtca
//
