LOCUS       NM_001168641            1978 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus tryptophanyl tRNA synthetase 2 (mitochondrial)
            (Wars2), mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_001168641 XM_001065089 XM_001075111
VERSION     NM_001168641.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Pravenec,M., Zidek,V., Landa,V., Mlejnek,P., Silhavy,J.,
            Simakova,M., Trnovska,J., Skop,V., Markova,I., Malinska,H.,
            Huttl,M., Kazdova,L., Bardova,K., Tauchmannova,K., Vrbacky,M.,
            Nuskova,H., Mracek,T., Kopecky,J. and Houstek,J.
  TITLE     Mutant Wars2 gene in spontaneously hypertensive rats impairs brown
            adipose tissue function and predisposes to visceral obesity
  JOURNAL   Physiol Res 66 (6), 917-924 (2017)
   PUBMED   29261326
  REMARK    GeneRIF: Wars2 gene regulates brown adipose tissue function and
            consequently lipid and glucose metabolism.
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Musante,L., Puttmann,L., Kahrizi,K., Garshasbi,M., Hu,H., Stehr,H.,
            Lipkowitz,B., Otto,S., Jensen,L.R., Tzschach,A., Jamali,P.,
            Wienker,T., Najmabadi,H., Ropers,H.H. and Kuss,A.W.
  TITLE     Mutations of the aminoacyl-tRNA-synthetases SARS and WARS2 are
            implicated in the etiology of autosomal recessive intellectual
            disability
  JOURNAL   Hum Mutat 38 (6), 621-636 (2017)
   PUBMED   28236339
REFERENCE   3  (bases 1 to 1978)
  AUTHORS   Wang,M., Sips,P., Khin,E., Rotival,M., Sun,X., Ahmed,R.,
            Widjaja,A.A., Schafer,S., Yusoff,P., Choksi,P.K., Ko,N.S.,
            Singh,M.K., Epstein,D., Guan,Y., Houstek,J., Mracek,T., Nuskova,H.,
            Mikell,B., Tan,J., Pesce,F., Kolar,F., Bottolo,L., Mancini,M.,
            Hubner,N., Pravenec,M., Petretto,E., MacRae,C. and Cook,S.A.
  TITLE     Wars2 is a determinant of angiogenesis
  JOURNAL   Nat Commun 7, 12061 (2016)
   PUBMED   27389904
  REMARK    GeneRIF: The data demonstrate a pro-angiogenic function for Wars2
            both within and outside the heart.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1978)
  AUTHORS   Banin,E., Dorrell,M.I., Aguilar,E., Ritter,M.R., Aderman,C.M.,
            Smith,A.C., Friedlander,J. and Friedlander,M.
  TITLE     T2-TrpRS inhibits preretinal neovascularization and enhances
            physiological vascular regrowth in OIR as assessed by a new method
            of quantification
  JOURNAL   Invest Ophthalmol Vis Sci 47 (5), 2125-2134 (2006)
   PUBMED   16639024
REFERENCE   5  (bases 1 to 1978)
  AUTHORS   Mootha,V.K., Bunkenborg,J., Olsen,J.V., Hjerrild,M.,
            Wisniewski,J.R., Stahl,E., Bolouri,M.S., Ray,H.N., Sihag,S.,
            Kamal,M., Patterson,N., Lander,E.S. and Mann,M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000002.1.
            
            On or before Dec 13, 2009 this sequence version replaced
            XM_001065089.1, XM_001075111.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643297.8749.1,
                                           SRR26643292.10043.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-100               JAXUCZ010000002.1  189150361-189150460
            101-358             JAXUCZ010000002.1  189196374-189196631
            359-439             JAXUCZ010000002.1  189217781-189217861
            440-525             JAXUCZ010000002.1  189220484-189220569
            526-644             JAXUCZ010000002.1  189228863-189228981
            645-1978            JAXUCZ010000002.1  189230524-189231857
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="2"
                     /map="2q34"
     gene            1..1978
                     /gene="Wars2"
                     /note="tryptophanyl tRNA synthetase 2 (mitochondrial)"
                     /db_xref="GeneID:690654"
                     /db_xref="RGD:1593417"
     exon            1..100
                     /gene="Wars2"
                     /inference="alignment:Splign:2.1.0"
     CDS             11..1093
                     /gene="Wars2"
                     /EC_number="6.1.1.2"
                     /codon_start=1
                     /product="tryptophan--tRNA ligase, mitochondrial"
                     /protein_id="NP_001162112.1"
                     /db_xref="GeneID:690654"
                     /db_xref="RGD:1593417"
                     /translation="MALFSVRKARECWRFIRALHKGPTATAAPQKESGERVFSGIQPT
                     GIPHLGNYLGAIESWVKLQEEYDTVIYSIVDLHSITVPQDPGILQQSILDMTAVLLAC
                     GIDPERSILFQQSQVSEHTQLSWILTCMVRLPRLQHLHQWKAKAARQKHDGTVGLLTY
                     PVLQAADILCYKSTHVPVGEDQVQHMELVQDLARSFNQKYGELFPLPRSILTSMKKVK
                     SLRDPSAKMSKSDPDKLATVQITDSPEEIVRKFRKAVTDFTSEVTYEPDSRPGVSNMV
                     AIHAAVSGLSVEEVVRNSAGVDTARYKLLVADAVIEKFAPIRSEIEKLKMDKDHLRKV
                     LLVGSAKAKELASPVFEEVKKLVGIL"
     exon            101..358
                     /gene="Wars2"
                     /inference="alignment:Splign:2.1.0"
     exon            359..439
                     /gene="Wars2"
                     /inference="alignment:Splign:2.1.0"
     exon            440..525
                     /gene="Wars2"
                     /inference="alignment:Splign:2.1.0"
     exon            526..644
                     /gene="Wars2"
                     /inference="alignment:Splign:2.1.0"
     exon            645..1978
                     /gene="Wars2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ccttctcaag atggcgctgt tctccgtgcg gaaggcgcgt gagtgctggc gcttcatccg
       61 ggcacttcat aaaggaccca cagctactgc ggctccccag aaggagagcg gggagcgagt
      121 gttctctggc attcagccta caggaatccc ccacctggga aattaccttg gagccatcga
      181 gagctgggtg aagttacagg aggagtatga cacggtgata tacagcattg tggacctcca
      241 ctccatcact gtcccccaag accccggcat cctgcagcag agcatcctgg acatgactgc
      301 cgtgctcctt gcctgcggca tagacccaga gagaagtatc cttttccagc agtctcaggt
      361 gtctgaacac actcagttaa gttggatcct cacctgcatg gtgagactgc ctcgactaca
      421 gcatttacac cagtggaagg cgaaggctgc gaggcagaag catgatggga cagtaggcct
      481 gctcacatac cctgtactcc aggcggcaga cattctgtgc tacaagtcca cacacgttcc
      541 tgtcggggaa gatcaagtcc agcacatgga actagttcag gatctagctc ggagtttcaa
      601 ccagaagtat ggcgagctct ttccactgcc caggtctatt ctcacatcca tgaagaaagt
      661 gaaatctctt cgtgaccctt ctgccaagat gtcaaaatcg gaccctgaca aactggccac
      721 tgtccaaata acagacagcc cagaggagat tgtacggaaa ttccgcaagg ctgtgacgga
      781 cttcacgtca gaagtcacct acgagccgga cagcagacct ggtgtctcca acatggtggc
      841 gatccatgcg gccgtgtcag gcctctcggt ggaggaggtg gtgcgcaata gtgcaggcgt
      901 ggacactgca cgctacaagc tgctagtggc cgacgctgtg attgagaaat ttgctccaat
      961 cagaagtgaa attgaaaaac tgaaaatgga taaggaccat ttaagaaagg ttttacttgt
     1021 gggatctgca aaagccaaag aattagcctc tcctgtgttc gaggaggtga agaagttggt
     1081 ggggattctg taggaaggtc agccagtcac tgcactccaa tgaaggcagc ttttctccca
     1141 cagattttag cctgtccata tttagttcag tttgatgatc agttgcattt gatgaagcca
     1201 tcaactgagc aatgttccag tcccatcagc aaatgctgta attgagagct gaggcaggca
     1261 cagctcttca gctcaagtta aacgaggccc attttttggt ctgaagtatt cccgcaaact
     1321 tgaacagtga ctgagccatg aagtgtgctt gctgtgcagt attcactgta tcaggtgcac
     1381 ttcatctgcg ctgtacaggc ccaagctata atccgagtcc agtgctattt gcagctcatt
     1441 aagaggtaga tttcacagct cacgtgtcac tgtgacgctg tttgtcctga tgtatttgaa
     1501 tgacttgttt cctgagaagc acacactgac ttatttcagc aaatgactgt tgttcagtga
     1561 ttcaggaggt gatcatggaa gaacattgct tcagctccag ggcctgcatt cctggttgta
     1621 ttcctaataa gcacttctat ttttacacgc atctcagctt agtctcacca agtgctccct
     1681 ttgtggaata cataggaacc gaggggcagg tctggcttgc acaccaaggc ctaatatcaa
     1741 gattgatttt gcagggtcag caagatggct cagcagtaaa gagctcttgc tgccaaccaa
     1801 acttgactat gtgagttcag tccctgagat ccacatgatt aaaggagaga accaactcct
     1861 caaagttgta ctgtaactta cacacacaca cacacacaca cacacacaca cacacacaca
     1921 caccacaatg aaatgcacac acctacccac acacagaata aataaatgtt tacttttg
//
