LOCUS       NM_001173511            3535 bp    mRNA    linear   ROD 28-MAR-2024
DEFINITION  Rattus norvegicus VPS50 subunit of EARP/GARPII complex (Vps50),
            mRNA.
ACCESSION   NM_001173511 XM_001064458 XM_231428
VERSION     NM_001173511.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3535)
  AUTHORS   Topalidou,I., Cattin-Ortola,J., Pappas,A.L., Cooper,K.,
            Merrihew,G.E., MacCoss,M.J. and Ailion,M.
  TITLE     The EARP Complex and Its Interactor EIPR-1 Are Required for Cargo
            Sorting to Dense-Core Vesicles
  JOURNAL   PLoS Genet 12 (5), e1006074 (2016)
   PUBMED   27191843
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3535)
  AUTHORS   Schindler,C., Chen,Y., Pu,J., Guo,X. and Bonifacino,J.S.
  TITLE     EARP is a multisubunit tethering complex involved in endocytic
            recycling
  JOURNAL   Nat Cell Biol 17 (5), 639-650 (2015)
   PUBMED   25799061
REFERENCE   3  (bases 1 to 3535)
  AUTHORS   Ghosh,D., Lippert,D., Krokhin,O., Cortens,J.P. and Wilkins,J.A.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   4  (bases 1 to 3535)
  AUTHORS   Gonzales,P.A., Pisitkun,T., Hoffert,J.D., Tchapyjnikov,D.,
            Star,R.A., Kleta,R., Wang,N.S. and Knepper,M.A.
  TITLE     Large-scale proteomics and phosphoproteomics of urinary exosomes
  JOURNAL   J Am Soc Nephrol 20 (2), 363-379 (2009)
   PUBMED   19056867
REFERENCE   5  (bases 1 to 3535)
  AUTHORS   Canestri,F.
  TITLE     A proposed clinical application of a model of CO2 laser radiation
            induced damage craters
  JOURNAL   J Med Eng Technol 12 (3), 112-117 (1988)
   PUBMED   3172165
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000004.1.
            
            On or before May 13, 2010 this sequence version replaced
            XM_001064458.2, XM_231428.5.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643298.21710.1,
                                           SRR26643297.15235.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-119               JAXUCZ010000004.1  32439149-32439267
            120-188             JAXUCZ010000004.1  32445460-32445528
            189-311             JAXUCZ010000004.1  32455389-32455511
            312-383             JAXUCZ010000004.1  32456606-32456677
            384-437             JAXUCZ010000004.1  32459176-32459229
            438-508             JAXUCZ010000004.1  32460123-32460193
            509-626             JAXUCZ010000004.1  32461958-32462075
            627-662             JAXUCZ010000004.1  32463435-32463470
            663-745             JAXUCZ010000004.1  32464838-32464920
            746-788             JAXUCZ010000004.1  32472487-32472529
            789-887             JAXUCZ010000004.1  32473666-32473764
            888-1028            JAXUCZ010000004.1  32476549-32476689
            1029-1161           JAXUCZ010000004.1  32485885-32486017
            1162-1253           JAXUCZ010000004.1  32488205-32488296
            1254-1348           JAXUCZ010000004.1  32492499-32492593
            1349-1447           JAXUCZ010000004.1  32492889-32492987
            1448-1538           JAXUCZ010000004.1  32499418-32499508
            1539-1715           JAXUCZ010000004.1  32502513-32502689
            1716-1834           JAXUCZ010000004.1  32505543-32505661
            1835-1941           JAXUCZ010000004.1  32509313-32509419
            1942-2063           JAXUCZ010000004.1  32521765-32521886
            2064-2144           JAXUCZ010000004.1  32524652-32524732
            2145-2293           JAXUCZ010000004.1  32529432-32529580
            2294-2390           JAXUCZ010000004.1  32531045-32531141
            2391-2549           JAXUCZ010000004.1  32531768-32531926
            2550-2671           JAXUCZ010000004.1  32535464-32535585
            2672-2861           JAXUCZ010000004.1  32537270-32537459
            2862-3535           JAXUCZ010000004.1  32539626-32540299
FEATURES             Location/Qualifiers
     source          1..3535
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="4"
                     /map="4q13"
     gene            1..3535
                     /gene="Vps50"
                     /gene_synonym="Ccdc132; RGD1304751"
                     /note="VPS50 subunit of EARP/GARPII complex"
                     /db_xref="GeneID:312083"
                     /db_xref="RGD:1304751"
     exon            1..119
                     /gene="Vps50"
                     /gene_synonym="Ccdc132; RGD1304751"
                     /inference="alignment:Splign:2.1.0"
     CDS             87..2981
                     /gene="Vps50"
                     /gene_synonym="Ccdc132; RGD1304751"
                     /note="coiled-coil domain-containing protein 132;
                     coiled-coil domain containing 132; EARP/GARPII complex
                     subunit VPS50; VPS50 EARP/GARPII complex subunit;
                     coiled-coil domain-containing protein 132-like;
                     syndetin-like"
                     /codon_start=1
                     /product="syndetin"
                     /protein_id="NP_001166982.1"
                     /db_xref="GeneID:312083"
                     /db_xref="RGD:1304751"
                     /translation="MQKIKSLMTRQGLKSPPESLNDLGAFESLRVPGKEEFRELREQP
                     SDPQAEQELINSIEQVYFSADPFDIVKYELEKLPPVLNLQELEEYRDKLKQQQAAVSK
                     KVADLILEKQPAYVKELERVTSLQTGLQLAAVICTNSRRHLNIAKEGFTQASLGLLAN
                     QRKRQLLIGLLKSLRTIKTLQRTDVRLSEMLEEEDYPGAIQLCLECQKAASTFKHYSC
                     ISELNSKLQDTLEQIEEQLDVALSKICKNFDVNHYTKVQQAYRLLGKTQTAMDQLHMH
                     FTQAIHNTVFQVVLGYVELCAGNTDTKFQKLQYKDLCTHVTPDSYIPCLADLCKALWE
                     VMLSYYRTMEWHEKHDSEETAAASEGSNVMSTEETNFDRGYVKKKLEHGLTRIWQDVQ
                     LKVKTYLLGTDLSIFKYDDFIFVLDIVSRLMQVGEEFCGSKSEVLQESIRKQSINYFK
                     NHHRIRLDELRMFLENETWELCPVKSNFSILQLHEFKFLEQSRSPSVSPSKQPSATSS
                     KPVTLFEQYCSGGNPFEIQADHKDEETEDVLASNGYESDEQEKSAYQDYDSDSDVPEE
                     LKRDYVDEQTGDVPVKSVSRETLKSRKKSDYSLNKVNAPILTNTTLNVIRLVGKYMQM
                     MNILKPIAFDVIHFMSQLFDYYLYAIYTFFGRNDSLESTGLGLSSSRLRTTLNRIQES
                     LIDLEGSADPTATLTAAEERKEKVPSPHLNQLVILTSGDTLYGLAERVVATESLVFLA
                     EQFEFLQPHLDAVMPAVKKPFLQQFYSQTVSTASELRKPIYWIVAGKAIDYEQMLLLM
                     TNVKWDVKEIMSQHNVYVDALLKEFEQFNKRLNEVSKRVRIPLPVSNILWEHCIRLAN
                     RTIVEGYANVKKCSNEGRALMQLDFQQFLMKLEKLTDIRPIPDKEFVEIYIKAYYLTE
                     NDMERWIKEHREYSTKQLTNLVNVCLGSHINKKARQKLLAAIDDIDRPKR"
     exon            120..188
                     /gene="Vps50"
                     /gene_synonym="Ccdc132; RGD1304751"
                     /inference="alignment:Splign:2.1.0"
     exon            189..311
                     /gene="Vps50"
                     /gene_synonym="Ccdc132; RGD1304751"
                     /inference="alignment:Splign:2.1.0"
     exon            312..383
                     /gene="Vps50"
                     /gene_synonym="Ccdc132; RGD1304751"
                     /inference="alignment:Splign:2.1.0"
     exon            384..437
                     /gene="Vps50"
                     /gene_synonym="Ccdc132; RGD1304751"
                     /inference="alignment:Splign:2.1.0"
     exon            438..508
                     /gene="Vps50"
                     /gene_synonym="Ccdc132; RGD1304751"
                     /inference="alignment:Splign:2.1.0"
     exon            509..626
                     /gene="Vps50"
                     /gene_synonym="Ccdc132; RGD1304751"
                     /inference="alignment:Splign:2.1.0"
     exon            627..662
                     /gene="Vps50"
                     /gene_synonym="Ccdc132; RGD1304751"
                     /inference="alignment:Splign:2.1.0"
     exon            663..745
                     /gene="Vps50"
                     /gene_synonym="Ccdc132; RGD1304751"
                     /inference="alignment:Splign:2.1.0"
     exon            746..788
                     /gene="Vps50"
                     /gene_synonym="Ccdc132; RGD1304751"
                     /inference="alignment:Splign:2.1.0"
     exon            789..887
                     /gene="Vps50"
                     /gene_synonym="Ccdc132; RGD1304751"
                     /inference="alignment:Splign:2.1.0"
     exon            888..1028
                     /gene="Vps50"
                     /gene_synonym="Ccdc132; RGD1304751"
                     /inference="alignment:Splign:2.1.0"
     exon            1029..1161
                     /gene="Vps50"
                     /gene_synonym="Ccdc132; RGD1304751"
                     /inference="alignment:Splign:2.1.0"
     exon            1162..1253
                     /gene="Vps50"
                     /gene_synonym="Ccdc132; RGD1304751"
                     /inference="alignment:Splign:2.1.0"
     exon            1254..1348
                     /gene="Vps50"
                     /gene_synonym="Ccdc132; RGD1304751"
                     /inference="alignment:Splign:2.1.0"
     exon            1349..1447
                     /gene="Vps50"
                     /gene_synonym="Ccdc132; RGD1304751"
                     /inference="alignment:Splign:2.1.0"
     exon            1448..1538
                     /gene="Vps50"
                     /gene_synonym="Ccdc132; RGD1304751"
                     /inference="alignment:Splign:2.1.0"
     exon            1539..1715
                     /gene="Vps50"
                     /gene_synonym="Ccdc132; RGD1304751"
                     /inference="alignment:Splign:2.1.0"
     exon            1716..1834
                     /gene="Vps50"
                     /gene_synonym="Ccdc132; RGD1304751"
                     /inference="alignment:Splign:2.1.0"
     exon            1835..1941
                     /gene="Vps50"
                     /gene_synonym="Ccdc132; RGD1304751"
                     /inference="alignment:Splign:2.1.0"
     exon            1942..2063
                     /gene="Vps50"
                     /gene_synonym="Ccdc132; RGD1304751"
                     /inference="alignment:Splign:2.1.0"
     exon            2064..2144
                     /gene="Vps50"
                     /gene_synonym="Ccdc132; RGD1304751"
                     /inference="alignment:Splign:2.1.0"
     exon            2145..2293
                     /gene="Vps50"
                     /gene_synonym="Ccdc132; RGD1304751"
                     /inference="alignment:Splign:2.1.0"
     exon            2294..2390
                     /gene="Vps50"
                     /gene_synonym="Ccdc132; RGD1304751"
                     /inference="alignment:Splign:2.1.0"
     exon            2391..2549
                     /gene="Vps50"
                     /gene_synonym="Ccdc132; RGD1304751"
                     /inference="alignment:Splign:2.1.0"
     exon            2550..2671
                     /gene="Vps50"
                     /gene_synonym="Ccdc132; RGD1304751"
                     /inference="alignment:Splign:2.1.0"
     exon            2672..2861
                     /gene="Vps50"
                     /gene_synonym="Ccdc132; RGD1304751"
                     /inference="alignment:Splign:2.1.0"
     exon            2862..3535
                     /gene="Vps50"
                     /gene_synonym="Ccdc132; RGD1304751"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gcttccctgt gttgtagctg gctgttgggt ggtcttaagt gatttgttac ttcccagcgg
       61 cagggtaatc gtctctcgag ttcgacatgc aaaaaatcaa atctcttatg acccggcagg
      121 gtctgaagag ccctccggag agcctcaatg atctgggtgc ctttgagagc cttcgggtcc
      181 ctggaaagga ggaattcagg gaacttcgag agcagccaag cgaccctcaa gctgaacaag
      241 agctaattaa tagtattgaa caagtttatt tttctgcgga tccatttgat attgttaaat
      301 atgagctgga gaagcttcct cctgttctca atctgcaaga gctagaggag tacagagaca
      361 aattaaagca acagcaagct gcagtctcta aaaaagtggc agatttgatt cttgagaaac
      421 agcctgctta tgtaaaggag cttgagagag ttacatcgct gcagacaggt cttcagttgg
      481 ctgctgttat ctgcacaaac agcaggagac acttgaatat tgcgaaggaa ggttttactc
      541 aggctagctt aggccttctt gcaaatcaaa ggaaacgtca gttgctgatt ggacttctga
      601 agtcactgag aaccattaaa acattgcaaa gaaccgatgt acgcttaagt gaaatgctgg
      661 aggaagagga ctatccagga gctattcagc tgtgcctgga atgtcagaag gctgccagca
      721 cttttaaaca ctacagctgt ataagtgagc tgaattcaaa gctgcaggat actttggagc
      781 aaattgagga acagttggat gtagctcttt ccaagatctg caagaatttt gacgttaacc
      841 attacaccaa ggttcagcaa gcttatcggc ttcttggaaa aacacagact gcaatggatc
      901 agcttcatat gcacttcact caagccattc acaacaccgt gtttcaagtt gtgcttggct
      961 atgtggagct gtgtgcagga aacacagata caaagttcca gaagcttcag tataaagatc
     1021 tctgtacgca cgttacacca gacagctata ttccatgcct ggcggacctg tgcaaagcac
     1081 tgtgggaagt catgctgagc tactacagaa ccatggagtg gcatgagaaa cacgacagtg
     1141 aggaaacagc tgcagcttct gaaggaagta atgtgatgag tactgaagaa accaattttg
     1201 atcgtggcta tgtaaaaaag aaattagagc atggccttac gcgaatatgg caggatgttc
     1261 aactaaaagt aaaaacctac ttgcttggaa ctgatttatc tattttcaaa tatgatgatt
     1321 tcatctttgt tctggatata gtcagcaggt tgatgcaagt tggagaagag ttttgtggta
     1381 gcaagtctga agttttgcag gaatccatta gaaaacaaag tatcaattac ttcaagaacc
     1441 accataggat aagacttgat gaactgagaa tgttcttaga gaatgagaca tgggagcttt
     1501 gccctgttaa gtcaaatttc agcatcttgc agcttcatga gtttaaattc ctggaacagt
     1561 ctcgatcccc atcagtttca cctagtaagc agccatcggc aacttcttcc aaacctgtga
     1621 ctttgtttga gcagtactgc agtggtggga atccatttga aattcaagct gaccacaaag
     1681 acgaagaaac agaagatgtc ttagcctcta atgggtacga atctgatgag caagagaaga
     1741 gtgcctatca ggactatgac agcgacagcg acgttcctga ggagctgaag cgcgattatg
     1801 tagatgagca aaccggcgat gtgcccgtaa agagtgtttc acgagagaca ctgaagagca
     1861 ggaagaaatc ggattacagt ctaaataaag tgaatgcacc catcttaaca aacacaacct
     1921 tgaatgtcat aaggcttgtt ggaaagtaca tgcagatgat gaacatcctt aagccaatcg
     1981 cctttgatgt tatccatttc atgtctcagc tctttgatta ttacttgtat gccatataca
     2041 ccttttttgg tcggaatgat tcattggaat caacaggact tggccttagt agtagcagac
     2101 taagaacaac tctaaacaga attcaggaaa gccttattga cctggagggc tcagctgatc
     2161 ctactgccac tctcacagca gccgaggaaa gaaaggagaa ggtacccagc ccacacctca
     2221 atcagctagt gattttgaca tctggggata cattgtatgg gttggcagag agagtggtag
     2281 ccacagaatc cttagtgttc ttggctgagc agttcgagtt ccttcaacca catttggatg
     2341 ctgtgatgcc tgcagtcaaa aagcccttcc tgcagcagtt ttactcccag acagtctcaa
     2401 ctgccagtga actgcgcaaa cccatttact ggattgtggc tggtaaagct attgattatg
     2461 aacagatgct tctcctaatg acaaatgtga agtgggatgt caaagagatc atgtcacagc
     2521 acaacgtcta tgtagatgca ttgctaaagg aatttgaaca gtttaacaag cggttaaatg
     2581 aagtttctaa gagagttcgg atacccttgc ctgtatcaaa tatactttgg gaacattgta
     2641 tacgattagc taacagaact attgtggaag ggtacgccaa tgtcaagaag tgcagtaatg
     2701 agggacgtgc cttgatgcaa ttggattttc aacagttttt aatgaaactc gagaaactaa
     2761 ctgacattag gcctattcct gataaagaat ttgtggaaat atatatcaaa gcctactacc
     2821 tgactgagaa tgacatggag cgttggatca aagagcaccg ggaatattca acgaagcagt
     2881 tgaccaacct ggtgaatgtt tgcctgggat ctcatattaa taagaaagca agacagaagc
     2941 tcctagcagc tatagatgat atagacagac ctaaacgata atgaacagag ctcttcctcg
     3001 atggcattga tcttctctca gcctgtacgt cacgaaaggc ggttcttctt gcactcctga
     3061 taattctcct ctaagaaaac ttggagcatg tccttttgag ctggaaaata gaaaatactc
     3121 aactgtgtgt ggtgcgctca agcgactttt atatgctcca gtttcttcag atattgctcc
     3181 tctttgttgt aaccttttcc tgaaatgatc ttattgtcat aactattatg tggctacaat
     3241 tatacagttt acaaaagagt gtaaatacat agttggaaga aacggtggct agtgtgtcta
     3301 tgaatagttt ttggagaatg ccttaataaa atgagaggat gagtgtacag tctttcctaa
     3361 ttatgagcat tgcatgagaa gtaaaaaaca tgtaagaaag tagttgaaaa atcagtatgt
     3421 tctctgtttt taactgtcag agcttctgcc agggctaatt tggttataac agtgatcata
     3481 aatgatgaaa tttaataaat gttactctaa aatgatcagt tgtatgagac tacct
//
