LOCUS       NP_663711                437 aa            linear   ROD 01-MAY-2025
DEFINITION  vacuolar protein sorting-associated protein 4A [Rattus norvegicus].
ACCESSION   NP_663711
VERSION     NP_663711.1
DBSOURCE    REFSEQ: accession NM_145678.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (residues 1 to 437)
  AUTHORS   Ren,J., Yuan,D., Xie,L., Tao,X., Duan,C., Bao,Y., He,Y., Ge,J. and
            Lu,H.
  TITLE     Up-regulation of Vps4A promotes neuronal apoptosis after
            intracerebral hemorrhage in adult rats
  JOURNAL   Metab Brain Dis 32 (2), 565-575 (2017)
   PUBMED   28064406
  REMARK    GeneRIF: Vps4A was involved in promoting neuronal apoptosis via
            inhibiting Akt phosphorylation after intracerebral hemorrhage
REFERENCE   2  (residues 1 to 437)
  AUTHORS   Thoresen,S.B., Campsteijn,C., Vietri,M., Schink,K.O., Liestol,K.,
            Andersen,J.S., Raiborg,C. and Stenmark,H.
  TITLE     ANCHR mediates Aurora-B-dependent abscission checkpoint control
            through retention of VPS4
  JOURNAL   Nat Cell Biol 16 (6), 550-560 (2014)
   PUBMED   24814515
REFERENCE   3  (residues 1 to 437)
  AUTHORS   Cashikar,A.G., Shim,S., Roth,R., Maldazys,M.R., Heuser,J.E. and
            Hanson,P.I.
  TITLE     Structure of cellular ESCRT-III spirals and their relationship to
            HIV budding
  JOURNAL   Elife 3, e02184 (2014)
   PUBMED   24878737
  REMARK    Publication Status: Online-Only
REFERENCE   4  (residues 1 to 437)
  AUTHORS   Sandrin,V. and Sundquist,W.I.
  TITLE     ESCRT requirements for EIAV budding
  JOURNAL   Retrovirology 10, 104 (2013)
   PUBMED   24107264
  REMARK    Publication Status: Online-Only
REFERENCE   5  (residues 1 to 437)
  AUTHORS   Baietti,M.F., Zhang,Z., Mortier,E., Melchior,A., Degeest,G.,
            Geeraerts,A., Ivarsson,Y., Depoortere,F., Coomans,C., Vermeiren,E.,
            Zimmermann,P. and David,G.
  TITLE     Syndecan-syntenin-ALIX regulates the biogenesis of exosomes
  JOURNAL   Nat Cell Biol 14 (7), 677-685 (2012)
   PUBMED   22660413
  REMARK    Publication Status: Online-Only
REFERENCE   6  (residues 1 to 437)
  AUTHORS   Tanaka,H., Fujita,H., Katoh,H., Mori,K. and Negishi,M.
  TITLE     Vps4-A (vacuolar protein sorting 4-A) is a binding partner for a
            novel Rho family GTPase, Rnd2
  JOURNAL   Biochem J 365 (Pt 2), 349-353 (2002)
   PUBMED   11931639
REFERENCE   7  (residues 1 to 437)
  AUTHORS   Garrus,J.E., von Schwedler,U.K., Pornillos,O.W., Morham,S.G.,
            Zavitz,K.H., Wang,H.E., Wettstein,D.A., Stray,K.M., Cote,M.,
            Rich,R.L., Myszka,D.G. and Sundquist,W.I.
  TITLE     Tsg101 and the vacuolar protein sorting pathway are essential for
            HIV-1 budding
  JOURNAL   Cell 107 (1), 55-65 (2001)
   PUBMED   11595185
REFERENCE   8  (residues 1 to 437)
  AUTHORS   Scheuring,S., Rohricht,R.A., Schoning-Burkhardt,B., Beyer,A.,
            Muller,S., Abts,H.F. and Kohrer,K.
  TITLE     Mammalian cells express two VPS4 proteins both of which are
            involved in intracellular protein trafficking
  JOURNAL   J Mol Biol 312 (3), 469-480 (2001)
   PUBMED   11563910
REFERENCE   9  (residues 1 to 437)
  AUTHORS   Howard,T.L., Stauffer,D.R., Degnin,C.R. and Hollenberg,S.M.
  TITLE     CHMP1 functions as a member of a newly defined family of vesicle
            trafficking proteins
  JOURNAL   J Cell Sci 114 (Pt 13), 2395-2404 (2001)
   PUBMED   11559748
REFERENCE   10 (residues 1 to 437)
  AUTHORS   Bishop,N. and Woodman,P.
  TITLE     ATPase-defective mammalian VPS4 localizes to aberrant endosomes and
            impairs cholesterol trafficking
  JOURNAL   Mol Biol Cell 11 (1), 227-239 (2000)
   PUBMED   10637304
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000019.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: FQ213218.1, FQ219248.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Rattus norvegicus"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="19"
                     /map="19q12"
     Protein         1..437
                     /product="vacuolar protein sorting-associated protein 4A"
                     /EC_number="3.6.4.6"
                     /note="vacuolar protein sorting 4a; vacuolar protein
                     sorting protein 4a"
                     /calculated_mol_wt=48776
     Region          4..348
                     /region_name="PTZ00454"
                     /note="26S protease regulatory subunit 6B-like protein;
                     Provisional"
                     /db_xref="CDD:240423"
     Site            8
                     /site_type="acetylation"
                     /note="N6-acetyllysine.
                     /evidence=ECO:0000250|UniProtKB:Q9UN37; propagated from
                     UniProtKB/Swiss-Prot (Q793F9.1)"
     Region          75..106
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q793F9.1)"
     Site            95
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (Q793F9.1)"
     Site            97
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (Q793F9.1)"
     Region          121..291
                     /region_name="RecA-like_VPS4"
                     /note="ATPase domain of vacuolar protein
                     sorting-associated protein 4; cd19521"
                     /db_xref="CDD:410929"
     Site            order(122,141,145,148..149,153,157,169..170,174,178,189,
                     191,193..195,198,206,227..228,231,236,238,240..242,
                     244..246,249..250,273,276)
                     /site_type="other"
                     /note="hexamer interface [polypeptide binding]"
                     /db_xref="CDD:410929"
     Site            order(128..130,132,169..175,227,272)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:410929"
     Region          374..434
                     /region_name="Vps4_C"
                     /note="Vps4 C terminal oligomerization domain; pfam09336"
                     /db_xref="CDD:462762"
     CDS             1..437
                     /gene="Vps4a"
                     /coded_by="NM_145678.2:171..1484"
                     /db_xref="GeneID:246772"
                     /db_xref="RGD:628810"
ORIGIN      
        1 mttstlqkai dlvtkateed kaknyeealr lyqhaveyfl haikyeahsd kakesirakc
       61 mqyldraekl kdylrnkekh gkkpvkenqs egkgsdsdse gdnpekkklq eqlmgavvme
      121 kpnirwndva glegakealk eavilpikfp hlftgkrtpw rgillfgppg tgksylakav
      181 ateannstff svsssdlmsk wlgeseklvk nlfelarqhk psiifidevd slcgsrnene
      241 seaarrikte flvqmqgvgn nndgtlvlga tnipwvldsa irrrfekriy iplpeeaara
      301 qmfrlhlgst phnltdanih elarktegys gadisiivrd slmqpvrkvq sathfkkvcg
      361 psrtnpsvmi ddlltpcspg dpgaiemtwm dvpgdkllep vvcmsdmlrs lattrptvna
      421 ddllkvkkfs edfgqes
//
