LOCUS       NM_022961               2168 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus VPS33A core subunit of CORVET and HOPS complexes
            (Vps33a), mRNA.
ACCESSION   NM_022961
VERSION     NM_022961.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Kondo,H., Maksimova,N., Otomo,T., Kato,H., Imai,A., Asano,Y.,
            Kobayashi,K., Nojima,S., Nakaya,A., Hamada,Y., Irahara,K.,
            Gurinova,E., Sukhomyasova,A., Nogovicina,A., Savvina,M.,
            Yoshimori,T., Ozono,K. and Sakai,N.
  TITLE     Mutation in VPS33A affects metabolism of glycosaminoglycans: a new
            type of mucopolysaccharidosis with severe systemic symptoms
  JOURNAL   Hum Mol Genet 26 (1), 173-183 (2017)
   PUBMED   28013294
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Hubert,V., Peschel,A., Langer,B., Groger,M., Rees,A. and Kain,R.
  TITLE     LAMP-2 is required for incorporating syntaxin-17 into
            autophagosomes and for their fusion with lysosomes
  JOURNAL   Biol Open 5 (10), 1516-1529 (2016)
   PUBMED   27628032
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2168)
  AUTHORS   Wartosch,L., Gunesdogan,U., Graham,S.C. and Luzio,J.P.
  TITLE     Recruitment of VPS33A to HOPS by VPS16 Is Required for Lysosome
            Fusion with Endosomes and Autophagosomes
  JOURNAL   Traffic 16 (7), 727-742 (2015)
   PUBMED   25783203
REFERENCE   4  (bases 1 to 2168)
  AUTHORS   Perini,E.D., Schaefer,R., Stoter,M., Kalaidzidis,Y. and Zerial,M.
  TITLE     Mammalian CORVET is required for fusion and conversion of distinct
            early endosome subpopulations
  JOURNAL   Traffic 15 (12), 1366-1389 (2014)
   PUBMED   25266290
REFERENCE   5  (bases 1 to 2168)
  AUTHORS   Jiang,P., Nishimura,T., Sakamaki,Y., Itakura,E., Hatta,T.,
            Natsume,T. and Mizushima,N.
  TITLE     The HOPS complex mediates autophagosome-lysosome fusion through
            interaction with syntaxin 17
  JOURNAL   Mol Biol Cell 25 (8), 1327-1337 (2014)
   PUBMED   24554770
REFERENCE   6  (bases 1 to 2168)
  AUTHORS   Gissen,P., Johnson,C.A., Gentle,D., Hurst,L.D., Doherty,A.J.,
            O'Kane,C.J., Kelly,D.A. and Maher,E.R.
  TITLE     Comparative evolutionary analysis of VPS33 homologues: genetic and
            functional insights
  JOURNAL   Hum Mol Genet 14 (10), 1261-1270 (2005)
   PUBMED   15790593
REFERENCE   7  (bases 1 to 2168)
  AUTHORS   Suzuki,T., Oiso,N., Gautam,R., Novak,E.K., Panthier,J.J.,
            Suprabha,P.G., Vida,T., Swank,R.T. and Spritz,R.A.
  TITLE     The mouse organellar biogenesis mutant buff results from a mutation
            in Vps33a, a homologue of yeast vps33 and Drosophila carnation
  JOURNAL   Proc Natl Acad Sci U S A 100 (3), 1146-1150 (2003)
   PUBMED   12538872
REFERENCE   8  (bases 1 to 2168)
  AUTHORS   Kim,B.Y., Kramer,H., Yamamoto,A., Kominami,E., Kohsaka,S. and
            Akazawa,C.
  TITLE     Molecular characterization of mammalian homologues of class C Vps
            proteins that interact with syntaxin-7
  JOURNAL   J Biol Chem 276 (31), 29393-29402 (2001)
   PUBMED   11382755
REFERENCE   9  (bases 1 to 2168)
  AUTHORS   Pevsner,J., Hsu,S.C., Hyde,P.S. and Scheller,R.H.
  TITLE     Mammalian homologues of yeast vacuolar protein sorting (vps) genes
            implicated in Golgi-to-lysosome trafficking
  JOURNAL   Gene 183 (1-2), 7-14 (1996)
   PUBMED   8996080
REFERENCE   10 (bases 1 to 2168)
  AUTHORS   Swank,R.T., Paigen,K. and Ganschow,R.E.
  TITLE     Genetic control of glucuronidase induction in mice
  JOURNAL   J Mol Biol 81 (2), 225-243 (1973)
   PUBMED   4777306
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC105752.1.
            
            On Aug 12, 2007 this sequence version replaced NM_022961.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC105752.1, SRR8487227.86607.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132262
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="12"
                     /map="12q16"
     gene            1..2168
                     /gene="Vps33a"
                     /gene_synonym="r-Vps33a"
                     /note="VPS33A core subunit of CORVET and HOPS complexes"
                     /db_xref="GeneID:65081"
                     /db_xref="RGD:620643"
     exon            1..118
                     /gene="Vps33a"
                     /gene_synonym="r-Vps33a"
                     /inference="alignment:Splign:2.1.0"
     CDS             17..1810
                     /gene="Vps33a"
                     /gene_synonym="r-Vps33a"
                     /note="vacuolar protein sorting 33A; VPS33A CORVET/HOPS
                     core subunit; vacuolar protein sorting protein 33a;
                     vacuolar protein sorting 33 homolog A"
                     /codon_start=1
                     /product="vacuolar protein sorting-associated protein 33A"
                     /protein_id="NP_075250.2"
                     /db_xref="GeneID:65081"
                     /db_xref="RGD:620643"
                     /translation="MAAHLSYGRVNLNVLREAVRRELREFLDKCAGSKAIVWDEYLTG
                     PFGLIAQYSLLKEHEVEKMFTLKGSRLPAADVKNIIFLVRPRLELMDMIAENVLSEDR
                     RGPTRDFHILFVPRRSLLCEQRLKDLGVLGSFIYREEYSLDLIPFDGDLLSMESESAF
                     KECYLEGDQTSLYHAAKGLMTLQALYGTIPQIFGKGECARQVANMMVRMKREFTGSQN
                     SVFPVFDNLLLLDRNVDLLTPLASQLTYEGLIDEIYGIQNSYVKLPPEKFAPKKQGGG
                     GGKDLPTEAKKLQLNSAEELYAEIRDKNFNAVGSVLSKKAKIISAAFEERHNAKTVGE
                     IKQFVSQLPHMQAARGSLANHTSIAELIKDVTTSEDFFDKLTVEQEFMSGIDTDKVNN
                     YIEDCIAQKHPLIKVLRLVCLQSVCNSGLKQKVLDYYKREILQTYGYEHILTLNNLEK
                     AGLLKAQTGGRNNYPTIRKTLRLWMDDVNEQNPTDISYVYSGYAPLSVRLAQLLSRPG
                     WRSIEEVLRILPGPHFEERQPLPTGLQKKRQPGENRVTLVFFLGGVTFAEIAALRFLS
                     QLEDGGTEYVIATTKLINGSSWLEALMEKPF"
     exon            119..184
                     /gene="Vps33a"
                     /gene_synonym="r-Vps33a"
                     /inference="alignment:Splign:2.1.0"
     exon            185..312
                     /gene="Vps33a"
                     /gene_synonym="r-Vps33a"
                     /inference="alignment:Splign:2.1.0"
     exon            313..499
                     /gene="Vps33a"
                     /gene_synonym="r-Vps33a"
                     /inference="alignment:Splign:2.1.0"
     exon            500..616
                     /gene="Vps33a"
                     /gene_synonym="r-Vps33a"
                     /inference="alignment:Splign:2.1.0"
     exon            617..791
                     /gene="Vps33a"
                     /gene_synonym="r-Vps33a"
                     /inference="alignment:Splign:2.1.0"
     exon            792..988
                     /gene="Vps33a"
                     /gene_synonym="r-Vps33a"
                     /inference="alignment:Splign:2.1.0"
     exon            989..1115
                     /gene="Vps33a"
                     /gene_synonym="r-Vps33a"
                     /inference="alignment:Splign:2.1.0"
     exon            1116..1183
                     /gene="Vps33a"
                     /gene_synonym="r-Vps33a"
                     /inference="alignment:Splign:2.1.0"
     exon            1184..1321
                     /gene="Vps33a"
                     /gene_synonym="r-Vps33a"
                     /inference="alignment:Splign:2.1.0"
     exon            1322..1459
                     /gene="Vps33a"
                     /gene_synonym="r-Vps33a"
                     /inference="alignment:Splign:2.1.0"
     exon            1460..1628
                     /gene="Vps33a"
                     /gene_synonym="r-Vps33a"
                     /inference="alignment:Splign:2.1.0"
     exon            1629..2113
                     /gene="Vps33a"
                     /gene_synonym="r-Vps33a"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cgggtggtgg agcaagatgg cggctcacct gtcatacggg cgagtgaacc tgaacgtgct
       61 gcgcgaggcg gtgcgtcggg agctgcgcga gtttctggac aagtgcgctg ggagcaaggc
      121 aatagtgtgg gatgaatact taacaggacc gtttggactg attgcacagt actcactact
      181 aaaggaacat gaagtggaaa aaatgttcac cctcaaagga agtcgtttgc cagcggctga
      241 tgtcaagaat atcatttttt tggtcagacc caggctagaa ttaatggata tgattgccga
      301 gaacgtactc agtgaggaca ggcgtggccc taccagggat ttccatattt tgtttgtgcc
      361 tcgtcggagc ctactgtgtg agcaaaggct gaaggacctg ggtgtgttgg gatcctttat
      421 ttatcgagag gagtacagtt tagatctcat tccatttgat ggggacctct tatccatgga
      481 gtcagagagc gctttcaaag aatgctacct tgaaggcgac cagacaagcc tgtaccacgc
      541 agccaagggg ctgatgaccc tgcaggcgct gtatggaact atcccccaga tctttgggaa
      601 aggagagtgt gctcggcaag tggccaatat gatggtcagg atgaaaaggg agtttacagg
      661 cagccaaaac tcagtgttcc ccgtgtttga taacctctta ctgcttgatc gcaacgtgga
      721 tctgctgacg ccgctcgcct cccagctcac gtacgaagga ctcatcgacg agatttacgg
      781 cattcagaac agttatgtga agttacctcc agagaaattt gcacccaaga agcagggcgg
      841 tggtggcggg aaggaccttc ccacagaggc aaagaagctt cagctcaact ccgcagagga
      901 actctatgcc gagatccgag acaagaactt caacgctgtg ggctccgtgc tcagcaagaa
      961 ggcgaagatc atctccgcgg cctttgagga gcgacacaat gctaagacgg ttggagagat
     1021 caagcaattc gtctcccagc ttccccacat gcaggcggcc agggggtcgc ttgccaacca
     1081 cacctcaatt gcagagttaa tcaaagacgt caccacttct gaagacttct ttgataagtt
     1141 gacggtggag caggagttca tgtccgggat agacaccgat aaggtcaaca attacatcga
     1201 ggactgcatt gcccaaaagc acccgctcat caaggtcctg agattggttt gcctccagtc
     1261 tgtgtgcaac agtgggctca agcagaaggt tctggactac tacaaaaggg aaattctcca
     1321 gacctacggc tatgagcaca tactgaccct gaacaacctg gagaaggccg gcctgctaaa
     1381 ggctcagaca gggggcagaa acaactaccc aacgattcgg aaaacattac gcctctggat
     1441 ggacgatgtc aatgaacaaa accccacaga catatcctac gtgtacagtg gttacgcacc
     1501 actcagcgtg cgactcgccc agctcctttc tcggccaggt tggaggagca tcgaggaggt
     1561 tctccgcatt ctcccagggc cccactttga agaaaggcag ccactgccca ccgggctgca
     1621 gaagaaacgc cagccaggag aaaacagagt cacactggtg tttttcctcg ggggcgtcac
     1681 ctttgctgaa atcgcagccc tgcgttttct ctcccagctg gaagacggag gcacagagta
     1741 tgtaattgcg accactaaac tgataaatgg aagcagctgg ctagaagctc tcatggaaaa
     1801 gcctttctag gctgtcagag gggacagtga gctgcccctg agaacgtgtt gcccaaagga
     1861 cataaagcca ggagcatgga ggcaacggga ttgtgttcct tcctgctcct ctctgtgctc
     1921 ctaaaccctg aagaagggag ggtcggcctg ggtaaggaca caatgagctg ggcgtggcgg
     1981 ctcatgccta gaactcctcc acttaagaga cacaggcagg aggatcgctg caagtttggg
     2041 acagctgggg ctacatagcg agttccaggc aaaagccctc ccttccagaa ataaaggaga
     2101 tcctgtttcg gaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     2161 aaaaaaaa
//
