LOCUS       NM_001106499            3880 bp    mRNA    linear   ROD 21-MAR-2023
DEFINITION  Rattus norvegicus VPS18 core subunit of CORVET and HOPS complexes
            (Vps18), mRNA.
ACCESSION   NM_001106499 XM_001080818 XM_230471
VERSION     NM_001106499.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3880)
  AUTHORS   Wartosch L, Gunesdogan U, Graham SC and Luzio JP.
  TITLE     Recruitment of VPS33A to HOPS by VPS16 Is Required for Lysosome
            Fusion with Endosomes and Autophagosomes
  JOURNAL   Traffic 16 (7), 727-742 (2015)
   PUBMED   25783203
REFERENCE   2  (bases 1 to 3880)
  AUTHORS   Perini ED, Schaefer R, Stoter M, Kalaidzidis Y and Zerial M.
  TITLE     Mammalian CORVET is required for fusion and conversion of distinct
            early endosome subpopulations
  JOURNAL   Traffic 15 (12), 1366-1389 (2014)
   PUBMED   25266290
REFERENCE   3  (bases 1 to 3880)
  AUTHORS   Pirooz SD, He S, Zhang T, Zhang X, Zhao Z, Oh S, O'Connell D,
            Khalilzadeh P, Amini-Bavil-Olyaee S, Farzan M and Liang C.
  TITLE     UVRAG is required for virus entry through combinatorial interaction
            with the class C-Vps complex and SNAREs
  JOURNAL   Proc Natl Acad Sci U S A 111 (7), 2716-2721 (2014)
   PUBMED   24550300
REFERENCE   4  (bases 1 to 3880)
  AUTHORS   Graham SC, Wartosch L, Gray SR, Scourfield EJ, Deane JE, Luzio JP
            and Owen DJ.
  TITLE     Structural basis of Vps33A recruitment to the human HOPS complex by
            Vps16
  JOURNAL   Proc Natl Acad Sci U S A 110 (33), 13345-13350 (2013)
   PUBMED   23901104
REFERENCE   5  (bases 1 to 3880)
  AUTHORS   Zlatic SA, Tornieri K, L'Hernault SW and Faundez V.
  TITLE     Clathrin-dependent mechanisms modulate the subcellular distribution
            of class C Vps/HOPS tether subunits in polarized and nonpolarized
            cells
  JOURNAL   Mol Biol Cell 22 (10), 1699-1715 (2011)
   PUBMED   21411634
REFERENCE   6  (bases 1 to 3880)
  AUTHORS   Xu L, Sowa ME, Chen J, Li X, Gygi SP and Harper JW.
  TITLE     An FTS/Hook/p107(FHIP) complex interacts with and promotes
            endosomal clustering by the homotypic vacuolar protein sorting
            complex
  JOURNAL   Mol Biol Cell 19 (12), 5059-5071 (2008)
   PUBMED   18799622
REFERENCE   7  (bases 1 to 3880)
  AUTHORS   Schroder B, Wrocklage C, Pan C, Jager R, Kosters B, Schafer H,
            Elsasser HP, Mann M and Hasilik A.
  TITLE     Integral and associated lysosomal membrane proteins
  JOURNAL   Traffic 8 (12), 1676-1686 (2007)
   PUBMED   17897319
REFERENCE   8  (bases 1 to 3880)
  AUTHORS   Kim BY, Sahara Y, Yamamoto A, Kominami E, Kohsaka S and Akazawa C.
  TITLE     The interaction of mammalian Class C Vps with nSec-1/Munc18-a and
            syntaxin 1A regulates pre-synaptic release
  JOURNAL   Biochem Biophys Res Commun 350 (3), 691-697 (2006)
   PUBMED   17027648
REFERENCE   9  (bases 1 to 3880)
  AUTHORS   Richardson SC, Winistorfer SC, Poupon V, Luzio JP and Piper RC.
  TITLE     Mammalian late vacuole protein sorting orthologues participate in
            early endosomal fusion and interact with the cytoskeleton
  JOURNAL   Mol Biol Cell 15 (3), 1197-1210 (2004)
   PUBMED   14668490
REFERENCE   10 (bases 1 to 3880)
  AUTHORS   Kim BY, Kramer H, Yamamoto A, Kominami E, Kohsaka S and Akazawa C.
  TITLE     Molecular characterization of mammalian homologues of class C Vps
            proteins that interact with syntaxin-7
  JOURNAL   J Biol Chem 276 (31), 29393-29402 (2001)
   PUBMED   11382755
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC169083.1.
            
            On Sep 4, 2008 this sequence version replaced NM_001106499.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC169083.1, SRR8487231.84183.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132262
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..3880
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="3"
                     /map="3q35"
     gene            1..3880
                     /gene="Vps18"
                     /note="VPS18 core subunit of CORVET and HOPS complexes"
                     /db_xref="GeneID:296083"
                     /db_xref="RGD:1311556"
     exon            1..347
                     /gene="Vps18"
                     /inference="alignment:Splign:2.1.0"
     CDS             257..3178
                     /gene="Vps18"
                     /note="vacuolar protein sorting 18 homolog; VPS18
                     CORVET/HOPS core subunit"
                     /codon_start=1
                     /product="vacuolar protein sorting-associated protein 18
                     homolog"
                     /protein_id="NP_001099969.1"
                     /db_xref="GeneID:296083"
                     /db_xref="RGD:1311556"
                     /translation="MASILDEYEDSLSRSAVLQTGCPSVGIPHSGYVNAHLEKEVPIF
                     TKQRIDFTPSERITSLVVSCSQLCMSLGKDTLLRIDLGKASEPNRVELGRKDDAKVHK
                     MFLDHTGSHLLVALSSTEVLYMNRNGQKARPLARWKGQLVESVGWNKALGSESSTGPI
                     LVGTAQGQIFEAELSASEGGLFGPAPDLYFRPLYVLNEEGGPAPVCSLEAERGPDGRG
                     FVIATTRQRLFQFIGRAVEDAEAQGFSGLFAAYTDHPPPFREFPGNLGYSELAFYTPK
                     LRSAPRAFAWMMGDGVLYGSLDCGRPDSLLSEEKVWEYPAGIGPGANPPLAIVLTQFH
                     FLLLLADRVEAVCTLTGQVVLRDHFLEKFGPLRHMVKDSSTGHLWAYTERAVFRYHVQ
                     REARDVWRTYLDMNRFDLAKEYCRERPDCLDTVLAREADFCFRQHRYLESARCYALTQ
                     SYFEEIALKFLEARQEEALAEFLQRKLAGLKPTERTQATLLTTWLTELYLSRLGALQG
                     DPDALNLYRDTRECFRTFLSSPRHKEWLFASRASIHELLASHGDTEHMVYFAVIMQDY
                     ERVVTYHCQHEAYEEALAVLARHRDPQLFYKFSPVLIRHIPRQLVDAWIEMGSRLDAR
                     QLIPALVNYSQGGDAQQVSQAIRYMEFCVNVLGETEQAIHNYLLSLYARGQPASLLAY
                     LEQAGASPHRVHYDLKYALRLCAEHGHHRACVHVYKVLELYEEAVDLALQVDVDLAKQ
                     CADLPEEDEELRKKLWLKIARHVVQEEEDVQTAMACLASCPLLKIEDVLPFFPDFVTI
                     DHFKEAICSSLKAYNHHIQELQREMEEATASAQRIRRDLQELRGRYGTVEPQDKCSTC
                     DFPLLNRPFYLFLCGHMFHADCLLQAVRPGLPTYKQARLEELQRKLGTAPPPTKGSVK
                     AKEAEAGAAAVGPSREQLKADLDELVAAECVYCGELMIRSIDRPFIDPQHYEEEHLSW
                     L"
     exon            348..489
                     /gene="Vps18"
                     /inference="alignment:Splign:2.1.0"
     exon            490..581
                     /gene="Vps18"
                     /inference="alignment:Splign:2.1.0"
     exon            582..2452
                     /gene="Vps18"
                     /inference="alignment:Splign:2.1.0"
     exon            2453..3777
                     /gene="Vps18"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 acgatcacct gggatctgct aaggggagcc tgtaatctct tgggactgga gctggggtgc
       61 caaactgcgc catattctag tgggggctcc tcgatcgtct cttcctatgc agagaaagcg
      121 aaaaaggcct cagagttttt aaaaggttct caggatctgt cagacgctgg gggaagcaca
      181 acttgcaaat ccaggacgac cggggtccca gttgtgaccc ccaggtccca gaccaaaggt
      241 ccagagtttg ataaccatgg cgtccatact ggatgagtat gaggactcat tgtcccgctc
      301 ggccgtcttg cagactggtt gccccagcgt gggcatcccc cattctgggt atgtaaatgc
      361 ccatctggag aaggaggtgc ctatcttcac aaagcagcgg attgacttca ccccctccga
      421 gcgaatcacc agccttgttg tttcctgcag tcagctctgt atgagcctgg gcaaggatac
      481 gctgctccgc attgacttgg gcaaggcaag cgaacccaac cgtgtggagc tgggccgcaa
      541 ggatgatgcc aaagtccaca agatgttcct ggaccacact ggctctcatc tgctggttgc
      601 tctgagtagc accgaggtcc tgtacatgaa ccgcaatgga cagaaggccc ggccgctggc
      661 tcgctggaag gggcagctgg tggagagtgt gggttggaac aaagcgctgg gcagtgagag
      721 cagcaccggc cccatcctgg ttggcacagc tcaaggacag atctttgaag cagagctctc
      781 agctagcgaa ggcggcctct ttggccctgc ccctgatctc tacttccgtc cactgtatgt
      841 cttaaatgaa gaagggggtc cagcacctgt gtgctccctt gaggcggagc gtggccctga
      901 cggacggggc tttgtcattg ctaccactcg gcagcgcctc ttccagttca taggccgagc
      961 agtagaagat gctgaagccc aaggcttctc gggcctcttt gcagcctata cagaccaccc
     1021 accgccattc cgtgagtttc ctggcaactt ggggtatagt gagttggctt tctatacccc
     1081 taagttacgc tctgcacccc gtgcctttgc ctggatgatg ggagatggtg tgctgtatgg
     1141 ctcactggac tgcgggcgcc ctgactccct gctgagcgag gagaaagtgt gggaataccc
     1201 agcagggatt ggtcctgggg ccaatccacc cttagccatc gtcttgaccc agttccattt
     1261 cctactgctg ctggcggacc gggtggaggc tgtgtgtact ctaacagggc aggtggtgct
     1321 acgggatcac ttcctggaga agtttggacc actgaggcac atggtgaagg actcgtccac
     1381 gggtcacctc tgggcctaca ctgagcgagc agtcttccga taccacgtgc aacgtgaggc
     1441 tcgggatgtc tggcgcacct acctggacat gaaccgcttt gacctggcca aagagtattg
     1501 tcgcgagcgg ccagattgcc tagacacagt cctggcccga gaggctgatt tctgctttcg
     1561 tcagcatcgc tacctggaga gcgcccgctg ctatgctctg acccagagct attttgagga
     1621 gattgccctc aagtttttgg aggcccggca agaagaggca ctggctgagt ttctgcagcg
     1681 gaaactggcc ggcttgaagc caactgagcg tactcaggcc acactactga ccacttggct
     1741 gacagagctc tacctgagcc ggcttggtgc tctgcagggt gacccagacg ccctgaatct
     1801 ctaccgggat acacgggagt gcttccgtac ttttctcagt agccctcggc acaaagagtg
     1861 gctctttgcc agccgggcct ctatccatga gctgcttgcc agtcacgggg acacagagca
     1921 tatggtgtat tttgcagtga tcatgcagga ctatgaacgg gtggtcacat accactgcca
     1981 gcatgaggcc tatgaggagg ccctggctgt gctagcccgc caccgagacc cccagctctt
     2041 ctacaaattc tcgcctgtcc tcatccgaca tatcccccgc cagctggtgg acgcctggat
     2101 tgagatgggc agccggctgg atgcccggca gctcattcct gccctggtga actatagcca
     2161 ggggggcgac gctcagcagg tgagccaagc catccgctat atggaattct gtgtgaatgt
     2221 gcttggcgag actgagcagg ccattcacaa ctacctgctg tcactgtatg cccgtggcca
     2281 gccagcctca cttctggcct acctggaaca agctggggcc agcccgcacc gtgtacacta
     2341 tgatctcaaa tatgcacttc gactttgtgc tgagcacggc caccaccgcg cctgcgtcca
     2401 tgtctacaag gtgttggagc tgtatgaaga ggctgtggac ctggccctgc aggtggatgt
     2461 ggacctggcc aagcagtgtg cagacctgcc agaggaggat gaggaacttc ggaagaaact
     2521 atggctcaag atcgctcggc atgtggtaca ggaggaggag gacgtacaga cagccatggc
     2581 ctgcttggcc agctgcccct tgctcaagat tgaggatgtg ttgcccttct ttcctgactt
     2641 tgtcacaatc gaccacttta aggaggcaat ctgtagttca cttaaggcct acaaccacca
     2701 tatccaggag ctacagcgcg agatggagga agccacagcc agcgcccagc gtatccgacg
     2761 agacttgcag gagcttcgag ggcgctatgg caccgtggag ccccaggaca aatgctccac
     2821 ctgtgacttc cctttgctca accgcccctt ttaccttttt ctctgtggcc acatgttcca
     2881 cgctgactgt ctgctgcagg ccgtgaggcc tggccttcct acctacaaac aggccaggct
     2941 tgaggagttg cagcgcaagc tcgggactgc gcctcctcca accaaaggct ctgtgaaggc
     3001 caaggaggcg gaagctgggg ctgcagctgt ggggcccagc cgggaacagc tcaaggctga
     3061 cctcgatgag ctggtggctg ctgagtgtgt gtactgtggg gagctgatga ttcgctccat
     3121 tgaccggccc ttcatcgacc cccagcacta tgaggaggaa cacctcagct ggctgtagga
     3181 agtgtcgcta tgcacgtgga cagacaggga tacagctacc agactcttct gggaaagcca
     3241 tgcctggcta gtgttgagtc cttgagattg ccaggtctgt ggttgtgagg gtgttgcagc
     3301 caagcctcag ccctgaggtt tcaggtgtga acccgttctg tgttctgcaa gctgtgcttc
     3361 agacctgact tagagctgct cctcccccag gcagcccgag ctggtgagat cagccgtgat
     3421 cctttcaact cactacatct aacagttcct ctgtcctcgt catagccagc atgttcacct
     3481 ggactgagta gcccactgtc catccttcct atcttccttc ctttgtcact ccacagcctc
     3541 tggacctccc actttgccct gccctcttct gccgggatcg agccatcagc cctccagatc
     3601 ctaggagagg aggcacttgt ggagagggtg gtactaaggg tgggcatgtg gacatgctgt
     3661 gtgaccctgg ggctccagtg tcagggtgcc ctgaggctga ggacacacca aggacaagag
     3721 gaaatagcaa caagcagaga gcattaaacg gtgccacagt ggtggctctg gacttgaaaa
     3781 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     3841 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
//
