LOCUS       NM_001005541            2641 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus VPS16 core subunit of CORVET and HOPS complexes
            (Vps16), mRNA.
ACCESSION   NM_001005541 XM_215829
VERSION     NM_001005541.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Wartosch,L., Gunesdogan,U., Graham,S.C. and Luzio,J.P.
  TITLE     Recruitment of VPS33A to HOPS by VPS16 Is Required for Lysosome
            Fusion with Endosomes and Autophagosomes
  JOURNAL   Traffic 16 (7), 727-742 (2015)
   PUBMED   25783203
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Perini,E.D., Schaefer,R., Stoter,M., Kalaidzidis,Y. and Zerial,M.
  TITLE     Mammalian CORVET is required for fusion and conversion of distinct
            early endosome subpopulations
  JOURNAL   Traffic 15 (12), 1366-1389 (2014)
   PUBMED   25266290
REFERENCE   3  (bases 1 to 2641)
  AUTHORS   Pirooz,S.D., He,S., Zhang,T., Zhang,X., Zhao,Z., Oh,S.,
            O'Connell,D., Khalilzadeh,P., Amini-Bavil-Olyaee,S., Farzan,M. and
            Liang,C.
  TITLE     UVRAG is required for virus entry through combinatorial interaction
            with the class C-Vps complex and SNAREs
  JOURNAL   Proc Natl Acad Sci U S A 111 (7), 2716-2721 (2014)
   PUBMED   24550300
REFERENCE   4  (bases 1 to 2641)
  AUTHORS   Graham,S.C., Wartosch,L., Gray,S.R., Scourfield,E.J., Deane,J.E.,
            Luzio,J.P. and Owen,D.J.
  TITLE     Structural basis of Vps33A recruitment to the human HOPS complex by
            Vps16
  JOURNAL   Proc Natl Acad Sci U S A 110 (33), 13345-13350 (2013)
   PUBMED   23901104
REFERENCE   5  (bases 1 to 2641)
  AUTHORS   Zlatic,S.A., Tornieri,K., L'Hernault,S.W. and Faundez,V.
  TITLE     Clathrin-dependent mechanisms modulate the subcellular distribution
            of class C Vps/HOPS tether subunits in polarized and nonpolarized
            cells
  JOURNAL   Mol Biol Cell 22 (10), 1699-1715 (2011)
   PUBMED   21411634
REFERENCE   6  (bases 1 to 2641)
  AUTHORS   Schroder,B., Wrocklage,C., Pan,C., Jager,R., Kosters,B.,
            Schafer,H., Elsasser,H.P., Mann,M. and Hasilik,A.
  TITLE     Integral and associated lysosomal membrane proteins
  JOURNAL   Traffic 8 (12), 1676-1686 (2007)
   PUBMED   17897319
REFERENCE   7  (bases 1 to 2641)
  AUTHORS   Kim,B.Y., Sahara,Y., Yamamoto,A., Kominami,E., Kohsaka,S. and
            Akazawa,C.
  TITLE     The interaction of mammalian Class C Vps with nSec-1/Munc18-a and
            syntaxin 1A regulates pre-synaptic release
  JOURNAL   Biochem Biophys Res Commun 350 (3), 691-697 (2006)
   PUBMED   17027648
REFERENCE   8  (bases 1 to 2641)
  AUTHORS   Richardson,S.C., Winistorfer,S.C., Poupon,V., Luzio,J.P. and
            Piper,R.C.
  TITLE     Mammalian late vacuole protein sorting orthologues participate in
            early endosomal fusion and interact with the cytoskeleton
  JOURNAL   Mol Biol Cell 15 (3), 1197-1210 (2004)
   PUBMED   14668490
REFERENCE   9  (bases 1 to 2641)
  AUTHORS   Kim,B.Y., Ueda,M., Nakamura,Y., Kohsaka,S. and Akazawa,C.
  TITLE     Expression of the mammalian homologue of vacuolar protein sorting
            16 (Vps16p) in the mouse and rat brain
  JOURNAL   Neurosci Lett 355 (3), 217-220 (2004)
   PUBMED   14732470
REFERENCE   10 (bases 1 to 2641)
  AUTHORS   Kim,B.Y., Kramer,H., Yamamoto,A., Kominami,E., Kohsaka,S. and
            Akazawa,C.
  TITLE     Molecular characterization of mammalian homologues of class C Vps
            proteins that interact with syntaxin-7
  JOURNAL   J Biol Chem 276 (31), 29393-29402 (2001)
   PUBMED   11382755
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC081963.1.
            
            On Oct 8, 2004 this sequence version replaced XM_215829.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC081963.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132262, SAMD00132263
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="3"
                     /map="3q36"
     gene            1..2641
                     /gene="Vps16"
                     /note="VPS16 core subunit of CORVET and HOPS complexes"
                     /db_xref="GeneID:296159"
                     /db_xref="RGD:1359414"
     exon            1..75
                     /gene="Vps16"
                     /inference="alignment:Splign:2.1.0"
     CDS             23..2542
                     /gene="Vps16"
                     /note="vacuolar protein sorting 16 homolog; VPS16
                     CORVET/HOPS core subunit"
                     /codon_start=1
                     /product="vacuolar protein sorting-associated protein 16
                     homolog"
                     /protein_id="NP_001005541.1"
                     /db_xref="GeneID:296159"
                     /db_xref="RGD:1359414"
                     /translation="MDCYTANWNPLGDSAFYRKYELYSMDWDLKEELKDCLVAAAPYG
                     GPIALLRNCWRKEKAASVRPVLEMYSASGMPLASLLWKSGPVVALGWSAEEELLCVQE
                     DGAVLVYGLHGDFRRHFSMGNEVLQNRVLDARIFHTEFGSGVAILTGAYRFTLSANVG
                     DLKLRRMPEVPGLQSAPSCWTTLCHERVAHILLAVGPDLYLLDHATCSAVTPAGLAPG
                     VSSFLQMAVSFTYRYLALFTDTGYIWMGTASLKEKLCEFNCNIRAPPKQMVWCSRPRS
                     KEKAVVVAWERRLMVVGNAPESIQFVLDEDSYLVPELDGVRIFSRSTHEFLHEVPVAS
                     EEIFKIASMAPGALLLEAQKEYEKESQKADEYLREIQELGQLIQAVQQCIEAAGHEHQ
                     PDMQKRLLRAASFGKCFLDRFPPDSFVHMCQDLRVLNAIRDYHIGIPLTYTQYKQLTI
                     QVLLDRLVLRRLYPLAIQICEYLRLPEVQGVSRILAHWACYKVQQKDVSDEDVARAIN
                     QKLGDTPGVSYSDIAARAYGCGRAELAIKLLEYEPRSGEQVPLLLKMKRSKLALSKAI
                     ESGDTDLVFTVLLHLKNELNRGDFFMTLRNQPMALSLYRQFCKHQELDTLKDLYNQDD
                     NHQELGSFHIRASYAAEERIEGRVAALQTAADAFYKAKNEFAAKATEDQMRLLRLQRR
                     LEDELGGRFLDLSLHDTVTTLILGGHNKRAEQLARDFRIPDKRLWWLKLSALADLEEW
                     EELEKFSKSKKSPIGYLPFVEICMKQHNKHEAKKYASRVGPEQKVKALLLVGDVAQAA
                     DVAIEHRNETELSLVLSHCTGTTDGAIADKIQRARAQAQKK"
     exon            76..164
                     /gene="Vps16"
                     /inference="alignment:Splign:2.1.0"
     exon            165..262
                     /gene="Vps16"
                     /inference="alignment:Splign:2.1.0"
     exon            263..391
                     /gene="Vps16"
                     /inference="alignment:Splign:2.1.0"
     exon            392..536
                     /gene="Vps16"
                     /inference="alignment:Splign:2.1.0"
     exon            537..652
                     /gene="Vps16"
                     /inference="alignment:Splign:2.1.0"
     exon            653..775
                     /gene="Vps16"
                     /inference="alignment:Splign:2.1.0"
     exon            776..831
                     /gene="Vps16"
                     /inference="alignment:Splign:2.1.0"
     exon            832..921
                     /gene="Vps16"
                     /inference="alignment:Splign:2.1.0"
     exon            922..1016
                     /gene="Vps16"
                     /inference="alignment:Splign:2.1.0"
     exon            1017..1093
                     /gene="Vps16"
                     /inference="alignment:Splign:2.1.0"
     exon            1094..1225
                     /gene="Vps16"
                     /inference="alignment:Splign:2.1.0"
     exon            1226..1353
                     /gene="Vps16"
                     /inference="alignment:Splign:2.1.0"
     exon            1354..1389
                     /gene="Vps16"
                     /inference="alignment:Splign:2.1.0"
     exon            1390..1498
                     /gene="Vps16"
                     /inference="alignment:Splign:2.1.0"
     exon            1499..1633
                     /gene="Vps16"
                     /inference="alignment:Splign:2.1.0"
     exon            1634..1742
                     /gene="Vps16"
                     /inference="alignment:Splign:2.1.0"
     exon            1743..1840
                     /gene="Vps16"
                     /inference="alignment:Splign:2.1.0"
     exon            1841..1948
                     /gene="Vps16"
                     /inference="alignment:Splign:2.1.0"
     exon            1949..2026
                     /gene="Vps16"
                     /inference="alignment:Splign:2.1.0"
     exon            2027..2196
                     /gene="Vps16"
                     /inference="alignment:Splign:2.1.0"
     exon            2197..2293
                     /gene="Vps16"
                     /inference="alignment:Splign:2.1.0"
     exon            2294..2397
                     /gene="Vps16"
                     /inference="alignment:Splign:2.1.0"
     exon            2398..2618
                     /gene="Vps16"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ctagccgaca gctgtactag acatggactg ttacactgcg aactggaatc cactcgggga
       61 ctctgccttt taccggaagt atgaactgta cagcatggac tgggacctga aggaggagct
      121 caaggattgt ctggtggctg ctgcacccta tgggggcccc attgctctac tgaggaactg
      181 ctggagaaaa gagaaagctg ccagtgtgcg gccagtactg gagatgtact ctgcttctgg
      241 catgcccctg gctagcctgc tgtggaagag tgggcctgtg gtggccctcg gctggtcagc
      301 cgaggaggag ctgctctgtg tgcaggaaga tggtgcagtg ctagtttatg ggcttcatgg
      361 agacttccgg aggcacttca gcatgggcaa tgaagtgctt cagaaccgtg tcttggatgc
      421 tcggattttt cacactgagt ttggttctgg ggtggccatc cttaccggag cctatcgatt
      481 tactctgagt gctaatgtgg gtgacctcaa actccgtcgg atgccagagg tgccaggtct
      541 gcagagtgca ccctcctgct ggaccacact gtgccacgag cgagtagcac acattcttct
      601 ggctgtagga cctgatcttt accttctgga tcatgctacc tgctctgcag tgacacctgc
      661 tggtctagct ccaggagtga gcagcttcct acagatggct gtatccttca cgtaccgtta
      721 cctggcactc ttcacagaca caggctacat ctggatgggg acagcatcac tcaaggagaa
      781 gctgtgtgag ttcaactgca acatccgggc acccccgaag cagatggtct ggtgtagccg
      841 tcctcgaagc aaggaaaagg ctgttgttgt agcctgggaa aggcggctga tggtggtggg
      901 caatgcgcct gaaagcattc agtttgtgct tgatgaggac tcctacttag tgcctgagct
      961 tgatggggtc cgcatcttct ctcgaagtac ccatgaattc ctgcatgaag tcccagtggc
     1021 cagtgaagag atcttcaaaa ttgcctcaat ggcccctgga gcactgctgt tggaggcaca
     1081 gaaggagtat gagaaagaga gccaaaaagc agatgaatat ctgcgggaga tccaggaact
     1141 ggggcagctg atccaggctg tgcagcagtg cattgaggct gcaggacatg agcaccaacc
     1201 agacatgcaa aagcgtctgc tcagggcggc ttcgtttgga aagtgtttcc ttgacagatt
     1261 tccgcctgac agcttcgtgc acatgtgtca ggaccttcga gtgctcaatg ctattaggga
     1321 ctaccacatt gggatccctc tgacctatac ccaatacaag cagcttacca tccaggtgct
     1381 gctcgacagg ctcgtgttgc ggaggcttta tcccctggct atccagatat gcgagtacct
     1441 gcgccttcct gaagtacagg gcgtcagcag gatcctagcc cactgggcct gctacaaggt
     1501 gcagcagaaa gatgtctcag atgaggacgt agcacgggct attaatcaga agctgggtga
     1561 cacgcctggt gtttcatact ctgacattgc tgcaagggcc tatggctgtg gccgcgctga
     1621 actggccatt aagctgctgg agtatgagcc acggtcaggg gaacaggtac ctcttctcct
     1681 gaagatgaag aggagcaaac tagctctgag caaggccatt gagagtggag acactgacct
     1741 ggtgtttaca gtgctgctgc acctgaagaa tgaactcaac cgaggagact ttttcatgac
     1801 tctgcggaac caacccatgg ccctgagttt gtatcgacag ttctgtaagc atcaggaact
     1861 agacactctg aaggaccttt acaatcagga cgacaatcac caggaattgg gcagcttcca
     1921 catcagagcc agctatgctg cggaagagcg tattgaaggg cgagtggcag ctctgcaaac
     1981 ggcagctgac gccttctaca aagccaagaa tgaatttgca gccaaggcta cagaggatca
     2041 aatgaggctc ctgcggctac agcgacgcct ggaggatgag ctagggggca ggttcctaga
     2101 cctatctcta catgacacag tcaccacgct tatcctcgga ggacacaaca agcgggcaga
     2161 gcagctggca cgtgactttc gcatccctga caagaggctc tggtggttaa agctgtctgc
     2221 cctggcagat ctggaagagt gggaggagct ggagaagttt tctaagagca agaaatcacc
     2281 aattggctac ctgccctttg tagagatctg catgaagcaa cataacaaac atgaggccaa
     2341 gaagtatgct tccagagtgg gccctgagca aaaggtcaag gctttgcttc ttgtcgggga
     2401 tgtggctcag gctgcagatg tggctattga gcaccggaat gaaactgagc tgagcctcgt
     2461 tttgtcccac tgcactggaa ctacagatgg ggccatagct gacaagattc agcgggccag
     2521 agcacaagcc cagaagaaat gaagtgcctg tccctaaagg ggctagttaa agtccagggg
     2581 tgtccagatg taaataaatt tggaatacta attcggaaaa aaaaaaaaaa aaaaaaaaaa
     2641 a
//
