LOCUS       NM_031836               3546 bp    mRNA    linear   ROD 11-SEP-2024
DEFINITION  Rattus norvegicus vascular endothelial growth factor A (Vegfa),
            transcript variant 1, mRNA.
ACCESSION   NM_031836
VERSION     NM_031836.3
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Kadekawa,K., Nishijima,S., Noguchi,K., Matsumoto,S. and Sugaya,K.
  TITLE     Elucidation of the pathophysiology of interstitial cystitis/bladder
            pain syndrome via experimental autoimmune cystitis rat model
  JOURNAL   Am J Physiol Regul Integr Comp Physiol 327 (2), R250-R260 (2024)
   PUBMED   38842512
REFERENCE   2  (bases 1 to 3546)
  AUTHORS   Fu,W., Ye,Y. and Hu,F.
  TITLE     LncRNA XIST promotes neovascularization in diabetic retinopathy by
            regulating miR-101-3p/VEGFA
  JOURNAL   Arch Endocrinol Metab 68, e230097 (2024)
   PUBMED   38739522
  REMARK    GeneRIF: LncRNA XIST promotes neovascularization in diabetic
            retinopathy by regulating miR-101-3p/VEGFA.
REFERENCE   3  (bases 1 to 3546)
  AUTHORS   Feng,C., Tian,Q., Tang,X., Yu,J., Li,H., Geng,C. and Xu,L.
  TITLE     microRNA-9a-5p disrupts the ELAVL1/VEGF axis to alleviate traumatic
            brain injury
  JOURNAL   Exp Neurol 375, 114721 (2024)
   PUBMED   38342180
  REMARK    GeneRIF: microRNA-9a-5p disrupts the ELAVL1/VEGF axis to alleviate
            traumatic brain injury.
REFERENCE   4  (bases 1 to 3546)
  AUTHORS   Li,Z., Xie,L., Zou,L., Xiao,S. and Tao,J.
  TITLE     Overexpression of RAD54L attenuates osteoarthritis by suppressing
            the HIF-1alpha/VEGF signaling pathway: Bioinformatics analysis and
            experimental validation
  JOURNAL   PLoS One 19 (4), e0298575 (2024)
   PUBMED   38593124
  REMARK    GeneRIF: Overexpression of RAD54L attenuates osteoarthritis by
            suppressing the HIF-1alpha/VEGF signaling pathway: Bioinformatics
            analysis and experimental validation.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3546)
  AUTHORS   Eswarappa,S.M., Potdar,A.A., Koch,W.J., Fan,Y., Vasu,K.,
            Lindner,D., Willard,B., Graham,L.M., DiCorleto,P.E. and Fox,P.L.
  TITLE     Programmed translational readthrough generates antiangiogenic
            VEGF-Ax
  JOURNAL   Cell 157 (7), 1605-1618 (2014)
   PUBMED   24949972
REFERENCE   6  (bases 1 to 3546)
  AUTHORS   Bastide,A., Karaa,Z., Bornes,S., Hieblot,C., Lacazette,E., Prats,H.
            and Touriol,C.
  TITLE     An upstream open reading frame within an IRES controls expression
            of a specific VEGF-A isoform
  JOURNAL   Nucleic Acids Res 36 (7), 2434-2445 (2008)
   PUBMED   18304943
REFERENCE   7  (bases 1 to 3546)
  AUTHORS   Meiron,M., Anunu,R., Scheinman,E.J., Hashmueli,S. and Levi,B.Z.
  TITLE     New isoforms of VEGF are translated from alternative initiation CUG
            codons located in its 5'UTR
  JOURNAL   Biochem Biophys Res Commun 282 (4), 1053-1060 (2001)
   PUBMED   11352659
REFERENCE   8  (bases 1 to 3546)
  AUTHORS   Terman,B.I., Dougher-Vermazen,M., Carrion,M.E., Dimitrov,D.,
            Armellino,D.C., Gospodarowicz,D. and Bohlen,P.
  TITLE     Identification of the KDR tyrosine kinase as a receptor for
            vascular endothelial cell growth factor
  JOURNAL   Biochem Biophys Res Commun 187 (3), 1579-1586 (1992)
   PUBMED   1417831
REFERENCE   9  (bases 1 to 3546)
  AUTHORS   de Vries,C., Escobedo,J.A., Ueno,H., Houck,K., Ferrara,N. and
            Williams,L.T.
  TITLE     The fms-like tyrosine kinase, a receptor for vascular endothelial
            growth factor
  JOURNAL   Science 255 (5047), 989-991 (1992)
   PUBMED   1312256
REFERENCE   10 (bases 1 to 3546)
  AUTHORS   Breier,G., Albrecht,U., Sterrer,S. and Risau,W.
  TITLE     Expression of vascular endothelial growth factor during embryonic
            angiogenesis and endothelial cell differentiation
  JOURNAL   Development 114 (2), 521-532 (1992)
   PUBMED   1592003
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from CO565920.1, BC168708.1,
            AF215725.1 and JAXUCZ010000009.1.
            
            On Nov 27, 2013 this sequence version replaced NM_031836.2.
            
            Summary: This gene is a member of the PDGF/VEGF growth factor
            family. It encodes a heparin-binding protein, which exists as a
            disulfide-linked homodimer. This growth factor induces
            proliferation and migration of vascular endothelial cells, and is
            essential for both physiological and pathological angiogenesis.
            Disruption of this gene in mice resulted in abnormal embryonic
            blood vessel formation. This gene is upregulated in many known
            tumors and its expression is correlated with tumor stage and
            progression. Alternatively spliced transcript variants encoding
            different isoforms have been found for this gene. There is also
            evidence for alternative translation initiation from upstream
            non-AUG (CUG) codons resulting in additional isoforms. A recent
            study showed that a C-terminally extended isoform is produced by
            use of an alternative in-frame translation termination codon via a
            stop codon readthrough mechanism, and that this isoform is
            antiangiogenic. Expression of some isoforms derived from the AUG
            start codon is regulated by a small upstream open reading frame,
            which is located within an internal ribosome entry site. [provided
            by RefSeq, Nov 2015].
            
            Transcript Variant: This variant (1) represents the longest
            transcript. This variant can initiate translation from non-AUG
            (CUG) site, and also from a downstream, in-frame AUG site. The
            isoform (1) represented in this RefSeq is derived from the CUG
            start codon and it is the longest isoform.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR21887622.1281030.1,
                                           SRR26360194.1524171.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760386, SAMEA5760389
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            non-AUG initiation codon :: PMID: 11352659, 11563986
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-124               CO565920.1         1-124
            125-1014            BC168708.1         1-890
            1015-1659           AF215725.1         1-645
            1660-3546           JAXUCZ010000009.1  22466308-22468194
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="9"
                     /map="9q12"
     gene            1..3546
                     /gene="Vegfa"
                     /gene_synonym="Vegf; VEGF-A; VEGF111; VEGF164; VPF"
                     /note="vascular endothelial growth factor A"
                     /db_xref="GeneID:83785"
                     /db_xref="RGD:619991"
     exon            1..1080
                     /gene="Vegfa"
                     /gene_synonym="Vegf; VEGF-A; VEGF111; VEGF164; VPF"
                     /inference="alignment:Splign:2.1.0"
     CDS             478..1659
                     /gene="Vegfa"
                     /gene_synonym="Vegf; VEGF-A; VEGF111; VEGF164; VPF"
                     /note="isoform 1 is encoded by transcript variant 1;
                     non-AUG (CUG) translation initiation codon; vascular
                     permeability factor"
                     /codon_start=1
                     /product="vascular endothelial growth factor A isoform 1"
                     /protein_id="NP_114024.2"
                     /db_xref="GeneID:83785"
                     /db_xref="RGD:619991"
                     /translation="MTDRQTDTAPSPSAHLLAGGQPTVDAAASREQEPKPAPGGGVEG
                     VGARGIARKLFVQLLGSSLSGVAVVCAAGGKPIGAGRSASSGLEKPGPEKRGEKEKEE
                     ERGPQWALGSREPGSWTGEAAVCADSAPAARAPQAPARASVPEGRGARQGAQESGLPR
                     SPSRRGSASRAGPGRASETMNFLLSWVHWTLALLLYLHHAKWSQAAPTTEGEQKAHEV
                     VKFMDVYQRSYCRPIETLVDIFQEYPDEIEYIFKPSCVPLMRCAGCCNDEALECVPTS
                     ESNVTMQIMRIKPHQSQHIGEMSFLQHSRCECRPKKDRTKPEKKSVRGKGKGQKRKRK
                     KSRFKSWSVHCEPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRCDKPR
                     R"
     exon            1081..1129
                     /gene="Vegfa"
                     /gene_synonym="Vegf; VEGF-A; VEGF111; VEGF164; VPF"
                     /inference="alignment:Splign:2.1.0"
     exon            1130..1326
                     /gene="Vegfa"
                     /gene_synonym="Vegf; VEGF-A; VEGF111; VEGF164; VPF"
                     /inference="alignment:Splign:2.1.0"
     exon            1327..1403
                     /gene="Vegfa"
                     /gene_synonym="Vegf; VEGF-A; VEGF111; VEGF164; VPF"
                     /inference="alignment:Splign:2.1.0"
     exon            1404..1433
                     /gene="Vegfa"
                     /gene_synonym="Vegf; VEGF-A; VEGF111; VEGF164; VPF"
                     /inference="alignment:Splign:2.1.0"
     exon            1434..1505
                     /gene="Vegfa"
                     /gene_synonym="Vegf; VEGF-A; VEGF111; VEGF164; VPF"
                     /inference="alignment:Splign:2.1.0"
     exon            1506..1637
                     /gene="Vegfa"
                     /gene_synonym="Vegf; VEGF-A; VEGF111; VEGF164; VPF"
                     /inference="alignment:Splign:2.1.0"
     exon            1638..3546
                     /gene="Vegfa"
                     /gene_synonym="Vegf; VEGF-A; VEGF111; VEGF164; VPF"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ttggggcagc cgagctgcag cgaggccgcg gcgctggggg cgagctgagc ggcggcagcg
       61 gagctctgtc gcgagacgca gcgacaaggc agactattca acggactcat cagccaggga
      121 gtctgtgctc tgggatttga tattcaaacc tctttttttt ttcttaaact gtattgtttt
      181 acgctttaat ttatttttgc ttcctattcc cctcttaaat cgtgccaacg gtttggagag
      241 gttgctcctt cactccctca aattacttcg gattttggaa atcagcagag gaaagaggta
      301 gcaggagctc cagagagaag tcaaggaaga gagagagaga gagagaccgg tcagagagcg
      361 cgctggcgag cgaacagaga gagggacagg ggcaaagtga ctgacctgct tttgggggtg
      421 accgccagag cgcggcgtga gccctccccc ttgggatctt tcatcggacc agtcgcgctg
      481 acggacagac agacagacac cgcccccagc cccagcgccc acctcctcgc cggcgggcag
      541 ccgacggtgg acgcggcggc gagccgcgag caggagccga agcccgcgcc cggaggcggg
      601 gtggaggggg tcggggctcg cgggattgca cggaaacttt tcgtccaact tctgggctct
      661 tctctctccg gagtagccgt ggtctgcgcc gcaggaggca aaccgatcgg agctgggaga
      721 agtgctagct cgggcctgga gaagccgggg cccgagaaga gaggggagaa agagaaggaa
      781 gaggagaggg ggccgcagtg ggcgctcggc tctcgggagc cgggctcatg gacgggtgag
      841 gcggcggtgt gcgcagacag tgctccagcc gcgcgcgcgc cccaggcccc ggcccgggcc
      901 tcggttccag aagggagagg agcccgccaa ggcgcgcaag agagcgggct gcctcgcagt
      961 ccgagccgga gagggagcgc gagccgcgcc ggccccggac gggcctctga aaccatgaac
     1021 tttctgctct cttgggtgca ctggaccctg gctttactgc tgtacctcca ccatgccaag
     1081 tggtcccagg ctgcacccac gacagaaggg gagcagaaag cccatgaagt ggtgaagttc
     1141 atggacgtct accagcgcag ctattgccgt ccaattgaga ccctggtgga catcttccag
     1201 gagtaccccg atgagataga gtatatcttc aagccgtcct gtgtgcccct aatgcggtgt
     1261 gcgggctgct gcaatgatga agccctggag tgcgtgccca cgtcggagag caacgtcact
     1321 atgcagatca tgcggatcaa acctcaccaa agccagcaca taggagagat gagcttcctg
     1381 cagcatagca gatgtgaatg cagaccaaag aaagatagaa caaagccaga aaaaaaatca
     1441 gttcgaggaa agggaaaggg tcaaaaacga aagcgcaaga aatcccggtt taaatcctgg
     1501 agcgttcact gtgagccttg ttcagagcgg agaaagcatt tgtttgtcca agatccgcag
     1561 acgtgtaaat gttcctgcaa aaacacagac tcgcgttgca aggcgaggca gcttgagtta
     1621 aacgaacgta cttgcagatg tgacaagcca aggcggtgag ccaggctgca ggaaggagcc
     1681 tccctcaggg tttcgggaac tagacctctc accggaaaga ccgattaacc atgtcaccac
     1741 cacaccacca tcgtcaccgt cgacagaaca gtccttaatc cagaaagcct gacatgaagg
     1801 gagaggagac tcttcgagga gcactttggg tccggagggc gagactccgg cagacgcatt
     1861 cccgggcagg tgaccaagca cggtggtccc tcgtggaact ggattcgcca ttttcttata
     1921 tttgctgcta aatcgccaag cccggaagat tagggagttt tgtttctggg attcctgtag
     1981 acacacccac ccacatacac acacatatat atatatatat tatatatata aataaatata
     2041 tatgttttat atataaaata tatatatatt cttttttttt ttaaattaac tctgctaatg
     2101 ttattggtgt cttcactgga tatgtttgac tgctgtggac ttgagttggg aggaggatgt
     2161 cctcacttgg atcccgacag ggaagacaat gggatgaaag actccggtgt ggtctttcgt
     2221 ccttcttaga gaggccgaag tctgtttgcc tgccagggag cacgcaaggc cagggcacgg
     2281 gggcacgttg gctcacttcc agaaacacga caaacccatc cctggccctg agtcaagagg
     2341 acagagagac agatgacaga taaagagata aagattctgg ttccgaccag acgtttttgg
     2401 ggagcctcag gacatggcac tattgtggat ccccactaga ttctgcaaga gcaccctgcc
     2461 cctctgggca ctgcctggaa gaatcaggag cctggccatc aagctctctc ctccacttct
     2521 gaggagccta ggaggcctcc cacaggggtc ctggcaaaga gaagacacag tggtggaaga
     2581 agaggcctgg taatggctcc tcctcctcct cctgggaacc cctcgtcctc tccctacccc
     2641 acttcctggg tatagctcag gaggaccttg tgtgatcaga ccattgaaac cactaattct
     2701 gtccccagga gacttggctg tgtgtgtgag tggcttaccc ttccccattt tcccttccca
     2761 aggtacagag caatggggca ggacccgcaa gcccctcatg gaggcagaga aaagagaaag
     2821 tgttttatat acggtactta tttaatagcc ctttttaatt agaaattaaa acagttaatt
     2881 taattaaaga gtagggtttt tttcagtatt cttggttaat atttaatttc aactatttat
     2941 gaggatgcat ctcttgctct ttcttatttg tactgttttt ttttgttttg tttttctgtg
     3001 tgtgtgtgtg tatgaaatct gtgtttccaa tctctctctc ccagatcggt gacagtcact
     3061 agcttgtcct gagaagatat ttaattttgc taacactcag ctctgccctc ccctgtcccc
     3121 accacacatt cctttgaaat aaggtttcaa tatacattta catactatat atatatttgg
     3181 caacttgtgt ttgtatataa atatatatat atatatatgt ttatgtatat atgtgattct
     3241 gataaaatag acattgctat tctgtttttt atatgtaaaa acaaaacgag aaaaaataga
     3301 gaattctaca tactaaatct ctctcctttt ttaattttaa tatttgttat catttattta
     3361 ttggtgctac tgtttatccg taataattgt gggggaaaag atattaacat cacgtctttg
     3421 tctctagagc agttttccga gatattccgt agtacatatt tatttttaaa cagcaacaaa
     3481 gaaatacaga tatatcttaa gaaaaaaaaa gcattttgta ttaaagaatt gaattctgat
     3541 ctcaaa
//
