LOCUS       NM_001107248            5270 bp    mRNA    linear   ROD 24-JUN-2024
DEFINITION  Rattus norvegicus vinculin (Vcl), mRNA.
ACCESSION   NM_001107248 XM_001057629 XM_223781
VERSION     NM_001107248.1
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 5270)
  AUTHORS   Shen,K., Kenche,H., Zhao,H., Li,J. and Stone,J.
  TITLE     The role of extracellular matrix stiffness in regulating
            cytoskeletal remodeling via vinculin in synthetic smooth muscle
            cells
  JOURNAL   Biochem Biophys Res Commun 508 (1), 302-307 (2019)
   PUBMED   30502091
  REMARK    GeneRIF: The vinculin-mediated mecahnotransduction mechanism in
            synthetic vascular smooth muscle cells may be more complex than
            those proposed for non-muscle cells.
REFERENCE   2  (bases 1 to 5270)
  AUTHORS   Vukelic,S., Xu,Q., Seidel-Rogol,B., Faidley,E.A., Dikalova,A.E.,
            Hilenski,L.L., Jorde,U., Poole,L.B., Lassegue,B., Zhang,G. and
            Griendling,K.K.
  TITLE     NOX4 (NADPH Oxidase 4) and Poldip2 (Polymerase delta-Interacting
            Protein 2) Induce Filamentous Actin Oxidation and Promote Its
            Interaction With Vinculin During Integrin-Mediated Cell Adhesion
  JOURNAL   Arterioscler Thromb Vasc Biol 38 (10), 2423-2434 (2018)
   PUBMED   30354218
  REMARK    GeneRIF: Integrin engagement during cell attachment activates
            Poldip2/Nox4 to oxidize actin, which modulates filamentous actin
            assembly and its interaction with vinculin.
REFERENCE   3  (bases 1 to 5270)
  AUTHORS   Rogg,M., Yasuda-Yamahara,M., Abed,A., Dinse,P., Helmstadter,M.,
            Conzelmann,A.C., Frimmel,J., Sellung,D., Biniossek,M.L., Kretz,O.,
            Grahammer,F., Schilling,O., Huber,T.B. and Schell,C.
  TITLE     The WD40-domain containing protein CORO2B is specifically enriched
            in glomerular podocytes and regulates the ventral actin
            cytoskeleton
  JOURNAL   Sci Rep 7 (1), 15910 (2017)
   PUBMED   29162887
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5270)
  AUTHORS   Birukova,A.A., Shah,A.S., Tian,Y., Moldobaeva,N. and Birukov,K.G.
  TITLE     Dual role of vinculin in barrier-disruptive and barrier-enhancing
            endothelial cell responses
  JOURNAL   Cell Signal 28 (6), 541-551 (2016)
   PUBMED   26923917
REFERENCE   5  (bases 1 to 5270)
  AUTHORS   Feutlinske,F., Browarski,M., Ku,M.C., Trnka,P., Waiczies,S.,
            Niendorf,T., Stallcup,W.B., Glass,R., Krause,E. and Maritzen,T.
  TITLE     Stonin1 mediates endocytosis of the proteoglycan NG2 and regulates
            focal adhesion dynamics and cell motility
  JOURNAL   Nat Commun 6, 8535 (2015)
   PUBMED   26437238
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5270)
  AUTHORS   Kawabe,H., Hata,Y., Takeuchi,M., Ide,N., Mizoguchi,A. and Takai,Y.
  TITLE     nArgBP2, a novel neural member of ponsin/ArgBP2/vinexin family that
            interacts with synapse-associated protein 90/postsynaptic
            density-95-associated protein (SAPAP)
  JOURNAL   J Biol Chem 274 (43), 30914-30918 (1999)
   PUBMED   10521485
REFERENCE   7  (bases 1 to 5270)
  AUTHORS   Watabe-Uchida,M., Uchida,N., Imamura,Y., Nagafuchi,A., Fujimoto,K.,
            Uemura,T., Vermeulen,S., van Roy,F., Adamson,E.D. and Takeichi,M.
  TITLE     alpha-Catenin-vinculin interaction functions to organize the apical
            junctional complex in epithelial cells
  JOURNAL   J Cell Biol 142 (3), 847-857 (1998)
   PUBMED   9700171
REFERENCE   8  (bases 1 to 5270)
  AUTHORS   van der Flier,A., Gaspar,A.C., Thorsteinsdottir,S., Baudoin,C.,
            Groeneveld,E., Mummery,C.L. and Sonnenberg,A.
  TITLE     Spatial and temporal expression of the beta1D integrin during mouse
            development
  JOURNAL   Dev Dyn 210 (4), 472-486 (1997)
   PUBMED   9415431
REFERENCE   9  (bases 1 to 5270)
  AUTHORS   Johnson,R.P. and Craig,S.W.
  TITLE     F-actin binding site masked by the intramolecular association of
            vinculin head and tail domains
  JOURNAL   Nature 373 (6511), 261-264 (1995)
   PUBMED   7816144
REFERENCE   10 (bases 1 to 5270)
  AUTHORS   Kieval,R.S., Bloch,R.J., Lindenmayer,G.E., Ambesi,A. and
            Lederer,W.J.
  TITLE     Immunofluorescence localization of the Na-Ca exchanger in heart
            cells
  JOURNAL   Am J Physiol 263 (2 Pt 1), C545-C550 (1992)
   PUBMED   1514597
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from CH474061.1.
            
            [WARNING] On Jul 16, 2024 this sequence was replaced by
            NM_001107248.2.
            
            On or before Oct 4, 2007 this sequence version replaced
            XM_223781.4, XM_001057629.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN16676785
                              [ECO:0000348]
            ##Evidence-Data-END##
FEATURES             Location/Qualifiers
     source          1..5270
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="15"
                     /map="15p16"
     gene            1..5270
                     /gene="Vcl"
                     /note="vinculin"
                     /db_xref="GeneID:305679"
                     /db_xref="RGD:1311217"
     exon            1..256
                     /gene="Vcl"
                     /inference="alignment:Splign:2.1.0"
     CDS             89..3289
                     /gene="Vcl"
                     /note="metavinculin"
                     /codon_start=1
                     /product="vinculin"
                     /protein_id="NP_001100718.1"
                     /db_xref="GeneID:305679"
                     /db_xref="RGD:1311217"
                     /translation="MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPV
                     AAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSV
                     PARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTY
                     TKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNS
                     KNQGIEEALKNRNFTVGKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALASIDSK
                     LNQAKGWLRDPNASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKMLGQMTDQ
                     VADLRARGQGASPVAMQKAQQVSQGLDVLTAKVENAARKLEAMTNSKQSIAKKIDAAQ
                     NWLADPNGGPEGEEQIRGALAEARKIAELCDDPKERDDILRSLGEIAALTSKLGDLRR
                     QGKGDSPEARALAKQVATALQNLQTKTNRAVANSRPAKAAVHLEGKIEQAQRWIDNPT
                     VDDRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLAAQLADLAARGEGES
                     PQARALASQLQDSLKDLKTQMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREE
                     VFDERAANFENHSGRLGATAEKAAAVGAANKSTVEGIQASVKTARELTPQVISAARIL
                     LRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVA
                     MANIQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMV
                     MDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTD
                     ELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSS
                     KGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQC
                     TDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNL
                     MQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ"
     exon            257..327
                     /gene="Vcl"
                     /inference="alignment:Splign:2.1.0"
     exon            328..478
                     /gene="Vcl"
                     /inference="alignment:Splign:2.1.0"
     exon            479..587
                     /gene="Vcl"
                     /inference="alignment:Splign:2.1.0"
     exon            588..710
                     /gene="Vcl"
                     /inference="alignment:Splign:2.1.0"
     exon            711..871
                     /gene="Vcl"
                     /inference="alignment:Splign:2.1.0"
     exon            872..962
                     /gene="Vcl"
                     /inference="alignment:Splign:2.1.0"
     exon            963..1110
                     /gene="Vcl"
                     /inference="alignment:Splign:2.1.0"
     exon            1111..1264
                     /gene="Vcl"
                     /inference="alignment:Splign:2.1.0"
     exon            1265..1440
                     /gene="Vcl"
                     /inference="alignment:Splign:2.1.0"
     exon            1441..1631
                     /gene="Vcl"
                     /inference="alignment:Splign:2.1.0"
     exon            1632..1831
                     /gene="Vcl"
                     /inference="alignment:Splign:2.1.0"
     exon            1832..1960
                     /gene="Vcl"
                     /inference="alignment:Splign:2.1.0"
     exon            1961..2110
                     /gene="Vcl"
                     /inference="alignment:Splign:2.1.0"
     exon            2111..2219
                     /gene="Vcl"
                     /inference="alignment:Splign:2.1.0"
     exon            2220..2522
                     /gene="Vcl"
                     /inference="alignment:Splign:2.1.0"
     exon            2523..2647
                     /gene="Vcl"
                     /inference="alignment:Splign:2.1.0"
     exon            2648..2833
                     /gene="Vcl"
                     /inference="alignment:Splign:2.1.0"
     exon            2834..3037
                     /gene="Vcl"
                     /inference="alignment:Splign:2.1.0"
     exon            3038..3142
                     /gene="Vcl"
                     /inference="alignment:Splign:2.1.0"
     exon            3143..5270
                     /gene="Vcl"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ctccgagtca gtctctcgcc gccggctccc ggccctacgg ttcccggttt tctgttgcct
       61 gcggttcgcc gccccgccag ccgccgcgat gccggtgttt cacacgcgca cgatcgagag
      121 catcctggag ccggtggcgc agcagatctc tcacctggtg attatgcacg aggagggcga
      181 ggtggacggc aaagccattc ctgacctcac cgcgcccgtg gccgctgtgc aggcggccgt
      241 cagcaacctc gtccgggttg ggaaagagac cgttcagacc actgaggatc agattttgaa
      301 gagagatatg ccgccagcat ttattaaggt cgagaatgct tgcacgaagc ttgttcaggc
      361 agctcagatg cttcagtcag acccatactc tgttcctgcg cgggattacc tcatcgacgg
      421 ctccagggga atcctctctg gaacgtctga cctcctgctt acctttgatg aggctgaggt
      481 tcgaaaaatt ataagagttt gcaaagggat tttggaatac ctcacagtgg cagaggtagt
      541 ggagactatg gaagacttgg tcacttacac aaagaacctt gggcccggaa tgacgaagat
      601 ggccaaaatg atcgatgaga gacagcagga gttgacgcac caggaacacc gtgtgatgct
      661 ggtgaactca atgaacactg tcaaagagct gcttcctgtt ctcatttcag ctatgaagat
      721 ttttgttaca accaaaaact caaaaaacca aggaatagaa gaagctctga aaaatcgaaa
      781 ttttactgta ggaaagatga gtgctgagat taacgagatc attcgtgtgt tacaactcac
      841 ctcctgggat gaggacgcct gggccagcaa ggacactgaa gccatgaaga gagcgctggc
      901 ctccatagac tcgaaactga accaggccaa aggttggctc cgtgacccca acgcttcccc
      961 aggggacgcc ggtgagcagg ccatcaggca gatcttggac gaagctggaa aagttggtga
     1021 actttgtgca ggcaaggaac gcagggagat cctaggaacc tgcaagatgc tagggcagat
     1081 gactgaccaa gtggctgacc tccgagccag aggacaagga gcctcgccag tggccatgca
     1141 gaaggcccag caagtgtctc aggggctcga cgtgcttact gcgaaagtgg aaaatgcagc
     1201 tcgaaagctg gaagccatga cgaactcaaa gcagagtatt gcgaagaaga ttgacgctgc
     1261 gcagaattgg ctggcagatc ccaatggtgg acctgaagga gaagaacaga tccgaggggc
     1321 tttggctgaa gctcggaaga ttgctgagtt atgtgatgat cctaaggaga gggacgacat
     1381 cctccgctct cttggagaga tagcggctct gacctctaaa ttaggagact tgcgaagaca
     1441 ggggaaagga gactccccag aggctcgagc cttggctaaa caagtggcaa ccgcgctaca
     1501 gaacctgcag accaaaacca atagggctgt ggccaacagc aggcctgcca aggcagccgt
     1561 ccacctggaa ggcaagattg agcaggcaca gcggtggatt gataacccta cagtggatga
     1621 ccgaggagtc ggtcaggctg ccatccgtgg acttgtggct gaagggcatc ggctggccaa
     1681 tgtcatgatg ggaccttatc gccaagatct tctcgccaag tgtgaccgtg tagaccagct
     1741 agcagctcag ctggctgacc tggctgcccg aggggagggg gagagtcctc aggcgagagc
     1801 gcttgcttct cagcttcagg actccttaaa ggatcttaaa acccagatgc aggaagctat
     1861 gactcaagag gtgtccgacg ttttcagcga tactacaact cccatcaagc tgttggcagt
     1921 ggcggccact gctcctcctg atgcgcccaa tagggaagag gtatttgacg agagagcagc
     1981 caactttgaa aaccattcag gaaggcttgg ggccacggca gagaaggcag ctgctgttgg
     2041 tgctgccaat aaatcgacag tggaaggcat tcaggcatcc gtgaagacag cccgagaact
     2101 cactccccag gtgatctcag ctgctcgaat cttactgagg aaccctggta accaagctgc
     2161 gtatgaacat tttgagacca tgaagaacca gtggattgat aatgttgaga aaatgacagg
     2221 gctggtggac gaggccattg ataccaaatc tctgttggat gcttctgaag aagcaattaa
     2281 aaaagacctg gacaagtgta aggtagccat ggccaatatt cagcctcaga tgctggtcgc
     2341 tggagcaacc agtattgctc gtcgggccaa ccggattctg ctggttgcta agagggaggt
     2401 agagaactct gaggatccga agttccgaga ggctgtgaaa gctgcctctg atgaactgag
     2461 caaaacaatc tcccccatgg ttatggatgc caaggctgtg gctggaaaca tctctgaccc
     2521 tggcctgcaa aagagcttcc tggactcagg atatcggatc ctgggagctg tggccaaagt
     2581 tagagaagcc ttccaacctc aggagcctga cttcccgcct cctccaccag accttgaaca
     2641 gctacgtcta actgatgagc tggctcctcc caaaccacct ctgcctgagg gtgaagtccc
     2701 tccacccagg ccacccccac cagaagagaa ggatgaagag ttccctgagc agaaagccgg
     2761 ggaggtgatt aaccagccga tgatgatggc cgccaggcag ctccacgatg aagctcggaa
     2821 gtggtctagc aagggcaatg acatcatcgc agcagccaag cgcatggctc tgctgatggc
     2881 ggagatgtct cggctggtaa gagggggcag tggtaccaag cgggcactta ttcagtgtgc
     2941 caaggatatc gccaaggcct ctgatgaggt gacgaggttg gccaaggagg ttgccaagca
     3001 gtgcacagat aagcggatta gaaccaatct cttacaggtc tgtgagcgaa tcccaactat
     3061 aagcacccag ctcaaaatcc tgtccacagt gaaggccacc atgctgggcc ggaccaacat
     3121 cagtgacgag gagtctgagc aggccacaga gatgctggtt cataatgccc agaacctcat
     3181 gcagtctgtg aaagagactg tgcgagaggc cgaagctgct tcaatcaaga ttcgaacaga
     3241 cgccggattt actctgcgct gggtcagaaa gactccctgg taccagtagg cacctggtca
     3301 gacctggctg gtacacagac ctctgctaat gaggaggtga ccatcttgag cttcagaagc
     3361 cattcagagt tgccaggggt ggaaaatcaa tccctggttt cacacaccag aaaggaatgg
     3421 ggcctcttca catagaaaaa acatttatac tcttgtcatg gacactttga agtgtctctc
     3481 tacaaaccct gtactttcag ttactctgtg cactctccca aggattttac ataagctcag
     3541 aataagcgtg aacactagca ggaagccctg ctctgccctg ggacatttct ctctgtgttt
     3601 atggttcact ggctgccttt ccccccttta ggctctcgag acctagaact gaggcgagtc
     3661 agtaaataac tgtgcttcca aagtgatttt gggtttcccc tttgctgctg accctgccca
     3721 tcttccctgg gctgtgcatc actgctgcag gaaaaggtgg tctctgggga gttccagctt
     3781 gcacagcctg gatccatttc ccactttctc cccatgtatt cctagttccg cacaatggaa
     3841 aaccaacttt cactgttagg aggaaatcac tcttctgtct ttaccatgcc cctcactagt
     3901 atgtgttatg gaaacttccc ttctcacatc ctgccttgct tggttgccaa ggtaaccatc
     3961 atgtaaacac actggtgctc tctgccactg agggggcaga gtagctgggt gtgatcctgc
     4021 ccatcctccc agtggggcca ctcccaggtt ttctatgctt gtcactgcca gtagaggtct
     4081 gtgctgaggc cttagcattc atccttagct tttactttct tttttaattt ctgagctgaa
     4141 atgctgcatt tacttttaac caaatcatcc taggcttggg cagtcttcct ctatgtgctt
     4201 cttaaacaag actttaaaga tcataggtgt gtgtgtctgc aatctctcca tcccccactt
     4261 tttttttttt ttttttttta attttgagac tgggtctctc tatgttgtcc aggctagtct
     4321 tgaacttctg gattcaagtc atctacttgt gtcagcctct tagctcctac caccacactt
     4381 gactttgctt gtaactttga aaagtccatt caaaattaag ctcttaagag actgaatgga
     4441 aaggcaattt tgtctgaagg atatttccta tgtaagggag aatagcattt gcagaatata
     4501 attctggtgc tgctagggaa aaatcagtag gaagttatag ttcccagttg cttttaacca
     4561 actacaacct tctctcaata taaagtattc aagaataaag agtatggtat ctacttatca
     4621 gaaaggcatg tttcctattg ggcaaagtta gtgaaaaagt gactttactc atttttgcat
     4681 ttacctcggc tgtataagca tttcctagcg catggatgct tcttccagaa atcaagaacc
     4741 aggtgaatac aggactaaga ccttcctgga tgttcttccc acatctagta tgttgacccc
     4801 aacactgaac ttgcaaatct taagttgacc ctgaatactc aggcttcccc tatttccttt
     4861 cagctgataa cagattcttt tgaaagctct cagcagatct gtatagttgc ttacctgata
     4921 taaatgcata tcaatgcctt taaagtatga atctatgcca aagatcatct tttgttttac
     4981 taaagattac ttagaggaat taagaaaaat gtttgctctc caagttcttc cagttctgga
     5041 gacatatgtc cgccagatgt gcttttctca agtgtcagtg ctcacagtac aaggaggcaa
     5101 aaggaaacag tccctgaacg acgtgttaga gatgccacca ctaaaaacta catttataag
     5161 ctaggactta catgcaaatg ttttctgcct cttctttcgt tctgtttaaa acaataaagt
     5221 acatttatat gaataatgct ctaaatgagg ttattttact aacgacttta
//
