LOCUS       NM_001170558           12281 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus versican (Vcan), transcript variant 1, mRNA.
ACCESSION   NM_001170558 XM_001058160 XM_001058357 XM_215451
VERSION     NM_001170558.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 12281)
  AUTHORS   Harten,I.A., Kaber,G., Agarwal,K.J., Kang,I., Ibarrientos,S.R.,
            Workman,G., Chan,C.K., Nivison,M.P., Nagy,N., Braun,K.R.,
            Kinsella,M.G., Merrilees,M.J. and Wight,T.N.
  TITLE     The synthesis and secretion of versican isoform V3 by mammalian
            cells: A role for N-linked glycosylation
  JOURNAL   Matrix Biol 89, 27-42 (2020)
   PUBMED   32001344
  REMARK    GeneRIF: The synthesis and secretion of versican isoform V3 by
            mammalian cells: A role for N-linked glycosylation.
REFERENCE   2  (bases 1 to 12281)
  AUTHORS   Bogen,O., Bender,O., Alvarez,P., Kern,M., Tomiuk,S., Hucho,F. and
            Levine,J.D.
  TITLE     Expression of a novel versican variant in dorsal root ganglia from
            spared nerve injury rats
  JOURNAL   Mol Pain 15, 1744806919874557 (2019)
   PUBMED   31429356
  REMARK    GeneRIF: mRNAs of the versican gene with exon 8beta are predicted
            to be constitutively degraded by nonsense-mediated RNA decay.
REFERENCE   3  (bases 1 to 12281)
  AUTHORS   Barallobre-Barreiro,J., Gupta,S.K., Zoccarato,A.,
            Kitazume-Taneike,R., Fava,M., Yin,X., Werner,T., Hirt,M.N.,
            Zampetaki,A., Viviano,A., Chong,M., Bern,M., Kourliouros,A.,
            Domenech,N., Willeit,P., Shah,A.M., Jahangiri,M., Schaefer,L.,
            Fischer,J.W., Iozzo,R.V., Viner,R., Thum,T., Heineke,J.,
            Kichler,A., Otsu,K. and Mayr,M.
  TITLE     Glycoproteomics Reveals Decorin Peptides With Anti-Myostatin
            Activity in Human Atrial Fibrillation
  JOURNAL   Circulation 134 (11), 817-832 (2016)
   PUBMED   27559042
REFERENCE   4  (bases 1 to 12281)
  AUTHORS   Barallobre-Barreiro,J., Oklu,R., Lynch,M., Fava,M., Baig,F.,
            Yin,X., Barwari,T., Potier,D.N., Albadawi,H., Jahangiri,M.,
            Porter,K.E., Watkins,M.T., Misra,S., Stoughton,J. and Mayr,M.
  TITLE     Extracellular matrix remodelling in response to venous
            hypertension: proteomics of human varicose veins
  JOURNAL   Cardiovasc Res 110 (3), 419-430 (2016)
   PUBMED   27068509
REFERENCE   5  (bases 1 to 12281)
  AUTHORS   Carthy,J.M., Abraham,T., Meredith,A.J., Boroomand,S. and
            McManus,B.M.
  TITLE     Versican localizes to the nucleus in proliferating mesenchymal
            cells
  JOURNAL   Cardiovasc Pathol 24 (6), 368-374 (2015)
   PUBMED   26395512
  REMARK    GeneRIF: Versican localizes to the nucleus in proliferating
            mesenchymal cells and suggest role in mitotic spindle organization
            during cell division.
REFERENCE   6  (bases 1 to 12281)
  AUTHORS   Lemire,J.M., Merrilees,M.J., Braun,K.R. and Wight,T.N.
  TITLE     Overexpression of the V3 variant of versican alters arterial smooth
            muscle cell adhesion, migration, and proliferation in vitro
  JOURNAL   J Cell Physiol 190 (1), 38-45 (2002)
   PUBMED   11807809
  REMARK    GeneRIF: Overexpression of alternatively spliced V3 variant of
            versican alters arterial smooth muscle cell adhesion, migration,
            proliferation and cell shape
REFERENCE   7  (bases 1 to 12281)
  AUTHORS   Lemire,J.M., Braun,K.R., Maurel,P., Kaplan,E.D., Schwartz,S.M. and
            Wight,T.N.
  TITLE     Versican/PG-M isoforms in vascular smooth muscle cells
  JOURNAL   Arterioscler Thromb Vasc Biol 19 (7), 1630-1639 (1999)
   PUBMED   10397680
REFERENCE   8  (bases 1 to 12281)
  AUTHORS   Mjaatvedt,C.H., Yamamura,H., Capehart,A.A., Turner,D. and
            Markwald,R.R.
  TITLE     The Cspg2 gene, disrupted in the hdf mutant, is required for right
            cardiac chamber and endocardial cushion formation
  JOURNAL   Dev Biol 202 (1), 56-66 (1998)
   PUBMED   9758703
REFERENCE   9  (bases 1 to 12281)
  AUTHORS   Milev,P., Maurel,P., Chiba,A., Mevissen,M., Popp,S., Yamaguchi,Y.,
            Margolis,R.K. and Margolis,R.U.
  TITLE     Differential regulation of expression of hyaluronan-binding
            proteoglycans in developing brain: aggrecan, versican, neurocan,
            and brevican
  JOURNAL   Biochem Biophys Res Commun 247 (2), 207-212 (1998)
   PUBMED   9642104
REFERENCE   10 (bases 1 to 12281)
  AUTHORS   Pyke,C., Kristensen,P., Ostergaard,P.B., Oturai,P.S. and Romer,J.
  TITLE     Proteoglycan expression in the normal rat kidney
  JOURNAL   Nephron 77 (4), 461-470 (1997)
   PUBMED   9434070
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AF072892.1, JAXUCZ010000002.1, AF062402.1 and AY007691.1.
            
            On or before May 29, 2010 this sequence version replaced
            XM_215451.6, XM_001058357.2, XM_001058160.1.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1, also referred to as
            V0).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data from several strains because no single
            transcript was available for the full length of the gene. The
            extent of this transcript is supported by transcript alignments and
            orthologous data.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMEA5760383, SAMEA5760386
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1322              AF072892.1         1-1322
            1323-3179           JAXUCZ010000002.1  22552729-22554585   c
            3180-10527          AF062402.1         1-7348
            10528-12281         AY007691.1         790-2543
FEATURES             Location/Qualifiers
     source          1..12281
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="WKY"
                     /db_xref="taxon:10116"
                     /chromosome="2"
                     /map="2q12"
     gene            1..12281
                     /gene="Vcan"
                     /gene_synonym="Cspg2"
                     /note="versican"
                     /db_xref="GeneID:114122"
                     /db_xref="RGD:619940"
     exon            1..274
                     /gene="Vcan"
                     /gene_synonym="Cspg2"
                     /inference="alignment:Splign:2.1.0"
     exon            275..350
                     /gene="Vcan"
                     /gene_synonym="Cspg2"
                     /inference="alignment:Splign:2.1.0"
     CDS             281..10354
                     /gene="Vcan"
                     /gene_synonym="Cspg2"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; versican core protein; GHAP; PG-M; large
                     fibroblast proteoglycan; glial hyaluronate-binding
                     protein; chondroitin sulfate proteoglycan core protein 2;
                     chondroitin sulfate proteoglycan 2 (versican)"
                     /codon_start=1
                     /product="versican core protein isoform 1 precursor"
                     /protein_id="NP_001164029.1"
                     /db_xref="GeneID:114122"
                     /db_xref="RGD:619940"
                     /translation="MLINMNGILWMCSTLLLTHALHKAKMEENPPVKGSLSGKVILPC
                     HFSTLPTLPPDYNTSEFLRIKWSKIEVDKNGKDIKETTVLVAQDGNIKIGQDYKGRVS
                     VPTHPDDVGDASLTMVKLRASDAGVYRCDVMYGIEDTQNTMSLAVDGVVFHYRAATSR
                     YTLNFESAQQACLDIGAVIATPEQLFAAYEDGFEQCDAGWLSDQTVRYPIRAPREGCY
                     GDMMGKEGVRTYGFRSPQETYDVYCYVDHLDGDVFHITAPSKFTFEEAEAECANRDAR
                     LATVGELHAAWRNGFDQCDYGWLSDASVRHPVTVARAQCGGGLLGVRTLYRFENQTCF
                     PLPDSRFDAYCFKPKQNISEATTIETNTLAETASPSLSKEPHMVPERATPVIPLVTEL
                     PIFTTHFPPAGNIVNSEQKSVIYSQAITDRVATESPTAAGSPINSWDADDYLPSGSGP
                     LGKPDISEIKEEGLQSTTVTSQHATASHHGITEDTQTHESVTQIEQIEVGPLVTSMEI
                     TNHISFKEFLETKTPLESTEVTLEQHQTDMPTVITSPELATTSHYGVTLREDDREDIA
                     LTVRSGQSTRVFSQIPEVITVSKTSEDTTYSQLGDLEFVSTATITMLGTDGSLTDEGK
                     EPQTDDKTAEDEFGQSQPTIPFPSQHLTEVESLPYSGDTISVERISTVSYPSLQTDVT
                     QGRERTEVPRPGLKKDPYAVDEIQEKITKDPFIGTIEEGFSGMSLSTSSSETSAERTE
                     SVSPALTIEKLTVKPAVASDVDEMTILTRLETDVPTSDEDVTSAHLTHSTLNVEVVTV
                     LKWPGNEDNSTSKPLPPTERAGFTKSPPVSLSTVGIVGKDKETPSFTDGGDEYTLSLD
                     GTPKPLEKFSEEDLTSGEFAVTIPTSISIDSAEKSTLGEPTTGDRVLSTTSTKDLVIN
                     ATVEGSALDEDMDASKPLFTATPFVHTSDVEESAFVNYSSTQQPTTYVDISHTSPLSI
                     IPKTEWSVSETSVPLEDEVLGKSDQDTLEQTHLEATMSPEALSTIEVTQGETQEEPQT
                     PGIPFPALSSTAVMTKETTAFEEEGEGSTYTLSEDRLMTDSEIVPSLETTPVGTSYPG
                     GAMTQQEVEMDTMVTQMSSIRPTVVLSTEPEVSYEAEGSSPMEFASTLKPFGTQVTQL
                     VEETTEEGKKTPLDYTDLGSGLFEQPRVTELPDFSMTPSDISVFTAIDSLHRTTPLRP
                     PSPFTEEPHIFEKEPSEKTTGDIILPRESVTQHPLTTLMDIIAKKTESDIDHEYHMTS
                     KPPVMQPTRPSVVERKTTSKPQELSTSPPPAGTKFHPDINVYIIEVRENKTGRMSDMV
                     VNGHPIDSESKEEEPCSEETDPLHDLFAEILPELPDSFEIDIYHSEEDEDGEEDCVNA
                     TDVTTTPSVQYITGKPHVTTVPKNPEAAEARRGLYESVAPSQNFSNTSATDTHQFIPA
                     ETELSTTMQFTKSKEATELLEITWKPETYPETPEHFSSGEPDVFPTLPSHDGKTTKWS
                     EFITESNPNTENPEHKQPKPIPLFPEEFSGEGAIDQASQQTIFSRATEVALGKETDQS
                     PTISTSSIRSGSVSVHALEEDPIALTGISQTDESMSTVESWVEMTPSQTVEFSGSSSA
                     PTIEGSGEVEEYTNKIFNTVTDLPQREPTDTLIPLDMSNIMITDHHIYTPATTAPLDS
                     QLPSTDARPTQFGIQTTTSEWVSSTSFEGRKTEEDKERDTNAAHTGEVQPATERSDRL
                     LLTSELESSNVAASSPLDTWEGFVPETTSTVSEKEMANTTPVFTETSDVANLETQSFE
                     HSSSSQPRVQEELTTLSGKPPLIFMDLGSGDASTDMEFITASSFTLDLESDTKVKKEL
                     PSTLSPSVETSSSSEPIGLAPSTVLDIEIVEVMNQTSKKTLISELSGKPTSQAEVRDL
                     YPGLGEDFSGDSSEYPTVSSTTMKEETVGMGGSENERVKDTQTLSSIPPTSDNINPVP
                     DSKGFGSTVASTTAFPWEEFMTSAEGSGEELSSVRSSVSLVLPLGVDILPTTESPYFD
                     QEFEEAAAVTEAGKQSALPIAVSGNTVDLTENRDIEVNSTMSVDLPQTMEPAKLWSKP
                     EVNPEKQEIGSETVTQDKAQGQKSFESLHSSLAPEQTTLESQSLIETEVQTSYYSMLT
                     TMKTYNTNEEVEEEGTSIAHMSTPGPGIKGLESYPTHPEATGKSYSFSASALVTESGP
                     ARSVVMDSSTQEEESIKLFQKDMILTHKESNSDLSFSGLGSGEALPPLPTTSVSLTDM
                     GKINSTLYPETSHMESLGTSILGDNHERMKNVSNEVRTLISETGSISQDSTEAPNTTL
                     SDTRTEESTTSPLPFMKLMDTEHSPKQTLRWEEEIQTHRPQTMTGQMTNDNSSVSEAE
                     AAATSAPAFLPETYSVEMTKAFATSPSQTSDLFDANSGEGSGEVDGLDLVYTSRTTQA
                     SSQGDSMFASHGFIEKHPEVSRTETGATDGSPTASAMFLHQSEYNESSLYPTSTLPST
                     VTYESPSEGIADGLQDHIRFEVSTLKPSRRKATESVIIDLDKEDSKDLGLAITESAIV
                     EILPELTSDRNIIIDIDHTKPVYEYIPGIQTDLDSDIPLGSHGSSEESLEVQEKYEAT
                     INLSPTEEAFDGSGDALPAGHTQAIYNESVTPSDGKQPEDISFSFATGIPVSSTETEL
                     NTFFPTVSTLHIPSKLTTASPEIDKPNIEAISLDDIFESSTLSDGQAIADQSEVISTL
                     GHLEKTQEEYEEKKYGGPSFQPEFFSGVGEVFTDAPAYVSIGRTYSVAQPLTEFPNVV
                     GQSDSTHYTEATSAVSSVTELSPQTPSSPSPVYIDSGVSEFTEVPHKSAQPAPTAASS
                     QKLIEGSFKKVRANIEATIKSLGENDHGTESPSMSPSPALDISEDDSKPKLLEDLETS
                     PTKTETSQDSPNKANDQIPGKTAGILAGIKTTESGPVVTAADDMELGDATQRPHSASA
                     PAAFRVETSMVPQPIPQEPERPTFPSLEINHETHTSLFEESILATSEKQVSQRILDYS
                     NQATVSTLDLNTEHSIPPFSILDNSNETAFLIGISEETVEGTAVYLPGPDLCKTNPCL
                     NGGTCYPTETSYVCTCAPGYSGDQCELDFDECHSNPCRNGATCVDGLNTFRCLCLPSY
                     VGALCEQDTETCDYGWHKFQGQCYKYFAHRRTWDAAERECRLQGAHLTSILSHEEQMF
                     VNRVGHDYQWIGLNDKMFEHDFRWTDGSALQYENWRPNQPDSFFSAGEDCVVIIWHEN
                     GQWNDVPCNYHLTYTCKKGTVACGQPPVVENAKTFGKMKPRYEINSLIRYHCKDGFIQ
                     RHLPTIRCLGNGRWAMPKITCMNPSAYQRTYSKKYLKNSSSVKDNSINTSKHEHRWSR
                     RWQETRR"
     exon            351..725
                     /gene="Vcan"
                     /gene_synonym="Cspg2"
                     /inference="alignment:Splign:2.1.0"
     exon            726..900
                     /gene="Vcan"
                     /gene_synonym="Cspg2"
                     /inference="alignment:Splign:2.1.0"
     exon            901..1028
                     /gene="Vcan"
                     /gene_synonym="Cspg2"
                     /inference="alignment:Splign:2.1.0"
     exon            1029..1322
                     /gene="Vcan"
                     /gene_synonym="Cspg2"
                     /inference="alignment:Splign:2.1.0"
     exon            1323..4220
                     /gene="Vcan"
                     /gene_synonym="Cspg2"
                     /inference="alignment:Splign:2.1.0"
     exon            4221..9428
                     /gene="Vcan"
                     /gene_synonym="Cspg2"
                     /inference="alignment:Splign:2.1.0"
     exon            9429..9542
                     /gene="Vcan"
                     /gene_synonym="Cspg2"
                     /inference="alignment:Splign:2.1.0"
     exon            9543..9656
                     /gene="Vcan"
                     /gene_synonym="Cspg2"
                     /inference="alignment:Splign:2.1.0"
     exon            9657..9815
                     /gene="Vcan"
                     /gene_synonym="Cspg2"
                     /inference="alignment:Splign:2.1.0"
     exon            9816..9898
                     /gene="Vcan"
                     /gene_synonym="Cspg2"
                     /inference="alignment:Splign:2.1.0"
     exon            9899..10043
                     /gene="Vcan"
                     /gene_synonym="Cspg2"
                     /inference="alignment:Splign:2.1.0"
     exon            10044..10226
                     /gene="Vcan"
                     /gene_synonym="Cspg2"
                     /inference="alignment:Splign:2.1.0"
     exon            10227..12270
                     /gene="Vcan"
                     /gene_synonym="Cspg2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gaacttgcag gcgagctgcc ccgagccttt ctgggtgaag aactcaaggc gcgcgggcgc
       61 agcagctgcg agcattaggt gctgaggacc ggcgccggaa ccgggatcag ccgcgagctg
      121 cgcatcctcc ctcctctcca gctctgtccc gcactcgccg catccttccc caggccaccg
      181 cgcttcctat gtgatctgcc ggggcaacgc ggagcccatt ctcacagctc agcagtgaat
      241 ctccccccca aactgcagta agccgccttt caaggacaag atgttgataa atatgaacgg
      301 catcctatgg atgtgctcaa ccttactgtt aacgcatgca ctgcataaag ccaaaatgga
      361 agaaaaccca cctgttaaag gctctctgtc tggaaaagtg atcctacctt gtcatttttc
      421 aaccttgccc accttaccac ccgattacaa cacgagtgaa tttctcagaa tcaaatggtc
      481 taaaatagaa gtggacaaaa atggaaaaga cataaaggag actactgtcc tggtggccca
      541 agacgggaac atcaagattg gtcaggacta caaggggcgg gtatcagtgc ctacgcatcc
      601 cgatgacgta ggcgatgcct ctctcaccat ggtcaaactc cgtgctagtg acgcaggtgt
      661 ctaccgctgt gatgtcatgt atggcattga agacactcag aacacgatgt cgctggccgt
      721 ggacggtgtc gtgtttcact acagggcagc gaccagcaga tacactctga acttcgagtc
      781 tgctcaacag gcttgtttgg acatcggggc ggtcatagca accccagagc agctgttcgc
      841 tgcctatgag gatggatttg agcagtgtga tgcaggatgg ctgtctgacc aaactgtcag
      901 atatcccata cgggctcccc gagagggctg ttatggagac atgatgggga aggaaggggt
      961 ccggacctat ggattccgct ctccccagga aacctatgat gtgtattgct atgtggatca
     1021 tctggacggc gatgtgttcc acatcactgc tcccagtaaa ttcaccttcg aggaggccga
     1081 agcagagtgt gcaaaccggg atgccaggct ggcgactgtt ggggaacttc acgcagcttg
     1141 gaggaacggc tttgaccagt gcgattacgg ctggctgtcg gatgccagcg tgcggcaccc
     1201 tgtgactgtg gccagggccc agtgtggagg tggtctactt ggggtgagaa ccctgtatcg
     1261 ttttgagaac cagacatgct tccctctccc tgatagcaga tttgatgcct actgctttaa
     1321 acctaaacag aatatatcag aggcaaccac catcgaaacg aataccctcg cagaaactgc
     1381 atcacccagt ttatccaaag aaccacacat ggtacccgag agagctacac cagtcattcc
     1441 attagtcacc gaattaccta tttttaccac acacttccct cctgcaggaa atatagtcaa
     1501 ttctgaacaa aaatctgtaa tctactctca ggctatcaca gacagagtag ccactgaatc
     1561 acccacagct gctgggagtc ccattaactc ttgggatgcg gatgactact tgccttctgg
     1621 gtcaggacca cttggaaagc cagacatatc tgaaattaag gaggaaggac ttcaaagtac
     1681 aactgtcacc tcccaacatg ctactgcctc acaccatgga attacagagg atacgcaaac
     1741 acatgaatca gttacacaga ttgaacaaat agaagtgggc cccttggtaa catccatgga
     1801 aatcacaaat cacatttctt tcaaggaatt tctagaaacc aaaacaccat tggaatccac
     1861 agaagtgacc ctggaacagc atcagaccga catgccaaca gttatcacca gtcctgaatt
     1921 ggcaaccaca agtcactatg gagtcacctt gagagaagat gatagagaag atatagcact
     1981 gacagtaaga tctggtcaga gtacccgggt ctttagccaa ataccagaag tcatcacggt
     2041 atcaaagaca tcagaagaca ctacctactc acagctaggg gacttagaat tcgtttcaac
     2101 agcaacaata accatgcttg gcacagatgg atcactgaca gatgaaggga aagaacccca
     2161 aactgatgat aagacggccg aagacgagtt tggtcaatca caacctacaa tccccttccc
     2221 atcacagcat cttacagaag tagaatcact tccatattct ggtgacacaa tatcagtaga
     2281 gagaatatcc acagttagtt atccatcatt acaaacagat gtgacacaag gaagagagag
     2341 aacagaagta ccaaggccag ggttaaaaaa agatccttat gcagttgatg agatacaaga
     2401 aaagatcacc aaagatccat ttataggtac aatagaagaa ggcttctctg gaatgtctct
     2461 ctctacatct tcctcagaga cttcagcgga aaggacggaa tctgtttccc cagcattgac
     2521 aattgagaag ttaactgtga agccagccgt agcaagcgat gtggacgaaa tgacaatcct
     2581 gactagatta gaaacagatg tgccaacgtc tgacgaagat gttacaagtg ctcatttgac
     2641 ccatagtact ttaaatgtgg aagtggtcac agtattaaaa tggccaggga atgaagacaa
     2701 ttcaacttca aagcctttac ccccaacaga acgtgcaggc tttacaaaat cgcctcctgt
     2761 ctcacttagc actgtaggaa tagttggaaa agataaagaa accccaagtt tcacagatgg
     2821 gggagacgaa tacactcttt ccctagatgg tactcccaag ccattagaaa agttttctga
     2881 ggaagattta acatctggag aatttgcagt tacaattcct acatctatat caattgacag
     2941 tgcagagaag tcaactttgg gagagcctac aactggagac agggttctgt ccaccacaag
     3001 cacaaaagat ctggtcatca atgcaactgt ggaaggaagt gctttggatg aagatatgga
     3061 tgcctccaag ccactgttta ctgccacccc atttgtccac acctccgatg tggaagaatc
     3121 ggcatttgtt aattatagta gcacccagca gcccactact tatgtagaca tatcccacac
     3181 tagtcctctt tctataattc ccaagacaga atggagtgta tcggaaactt ctgttccact
     3241 ggaagatgag gttctaggca aatctgatca agataccctt gaacagacac atctagaagc
     3301 aaccatgtct cctgaagctt tgagtacaat agaagtcaca cagggagaaa cccaggaaga
     3361 accacaaaca ccaggaatcc catttcctgc tctcagttct actgcagtta tgaccaagga
     3421 gacaacagca tttgaagaag agggggaggg gtcaacatat accctctctg aagacagatt
     3481 gatgacagat tctgaaatag tcccaagttt agaaacaact ccagttggta catcttatcc
     3541 aggtggtgca atgacacagc aggaagtaga aatggataca atggtaacac agatgtcaag
     3601 cattaggcca acagtagttt taagtactga gccagaagta agttatgaag cagaaggtag
     3661 tagtccaatg gaatttgcat cgactttgaa gccctttggg actcaagtca cacagcttgt
     3721 ggaagaaacg actgaggaag ggaaaaagac acccttggat tatacagatt taggttcagg
     3781 gttatttgaa caaccgagag ttactgagct cccagatttt tcaatgactc caagtgatat
     3841 cagtgtcttc actgccatcg acagccttca cagaaccaca cccttgaggc caccttcgcc
     3901 attcactgag gaaccacaca tctttgagaa ggagcccagt gagaaaacaa caggtgacat
     3961 aatacttcct agagaatctg taacacagca ccctctgaca actctcatgg atatcatagc
     4021 caagaaaaca gaatctgata ttgatcacga gtatcacatg acctcaaagc ctcctgtaat
     4081 gcagccaaca agaccatcag ttgtggaaag aaaaacaact tccaaacctc aagagttgtc
     4141 tacttcacca cccccggcag ggaccaaatt ccaccctgac ataaatgttt atattattga
     4201 ggtcagagaa aacaagacag gtcgaatgag tgatatggtt gtaaatggtc atccaataga
     4261 ttcagaatct aaagaagagg aaccttgcag tgaagaaaca gatccactgc atgatctgtt
     4321 tgctgaaatt ttacccgagt taccagattc cttcgaaata gacatatatc acagtgagga
     4381 agatgaagat ggagaggagg actgtgtaaa tgcaactgat gtaacaacca ctccgtcagt
     4441 gcagtacatc actgggaagc cacatgtcac tacagtgccc aaaaacccag aagctgcaga
     4501 agcgaggcgt ggcctgtatg aaagtgttgc gccttctcag aatttctcaa atacttccgc
     4561 aactgacacc catcagttta taccggccga aacagaattg tcaactacca tgcaatttac
     4621 caaatccaaa gaagccactg aattgttaga aatcacatgg aaacccgaga cctaccctga
     4681 aacaccagaa cacttttcaa gtggtgagcc tgatgttttc cctacactgc catcccatga
     4741 tggtaaaacc accaaatggt cagagttcat cacagagagc aatccaaaca cagaaaatcc
     4801 agaacacaaa caacctaagc ctatacctct gtttcctgaa gagttttcag gagagggtgc
     4861 cattgaccaa gcatcccagc aaacaatctt ttccagggct acagaagtag cacttggtaa
     4921 agagacagac caaagtccta ccatatctac ctctagcata cgttcaggtt ctgtgtcagt
     4981 acatgccttg gaggaagatc caattgctct aacaggaatc tcacagactg atgaatccat
     5041 gtctactgtg gaaagctggg tagagatgac ccctagccaa actgtagagt tctctgggag
     5101 ttcttcagct cctactattg aagggtctgg ggaagtagaa gaatatacaa ataaaatttt
     5161 caacacggta actgatttgc cacagagaga gcctacagat acgctcatcc ccttggatat
     5221 gagcaacatc atgatcacag accaccacat ttatactcct gcaaccaccg ctcctttgga
     5281 ttcacagcta ccttctacag atgcacggcc tacacaattt ggaattcaaa ccaccacctc
     5341 tgagtgggtt tccagtacat cttttgaagg aaggaaaaca gaggaagaca aagaaagaga
     5401 tacaaatgca gcccacacag gggaagtcca accagcgaca gagaggtcag atcgcttact
     5461 cttaacttca gaattagaaa gttcaaatgt ggctgcatct agtcctttgg acacctggga
     5521 aggttttgtg ccagagacaa catcaacagt gtccgaaaag gaaatggcaa acacaactcc
     5581 tgtttttaca gaaacaagtg atgtagccaa tttggagaca cagtcttttg agcatagtag
     5641 tagcagtcag cccagggtac aggaagagct gaccactctc tcaggaaagc cccccttgat
     5701 ttttatggac ctgggttcag gagatgctag tactgatatg gaattcatca ctgcttcttc
     5761 atttacatta gatttggagt ccgataccaa agtcaaaaaa gaattaccca gtactctgtc
     5821 tcccagtgtg gagacttcat cctcctctga gccaatagga ttagctccga gtactgtatt
     5881 ggacatagaa attgtggaag ttatgaatca gacatccaag aagacactga tttctgaact
     5941 atcaggaaag ccaacctccc aagcagaagt aagggattta tatccaggtc tgggagaaga
     6001 ttttagtggt gactccagtg aatatccaac agtgtcttct acaacaatga aagaggaaac
     6061 agtggggatg ggaggctctg agaatgaacg agttaaggat acacagactt tatcatctat
     6121 accacctacc tcagacaaca tcaacccagt gcctgactca aaaggattcg gcagtactgt
     6181 ggccagcacc acagccttcc cttgggaaga gttcatgacc tcagctgagg gctcaggtga
     6241 ggagctgtcc tcagtcagga gctctgttag tctagtgctt ccactaggtg tggacattct
     6301 tcctactaca gagtctccat attttgatca agagtttgaa gaagcggctg cagtgacaga
     6361 agcaggtaaa caatctgctt tgccaatagc agtgagtgga aatactgtag atctgacaga
     6421 aaacagggac atagaggtaa acagcacaat gtcagtagac cttccccaga caatggagcc
     6481 agccaaatta tggtctaaac cagaagtcaa tcctgaaaaa caagagattg gaagcgaaac
     6541 agtgacacag gataaagctc agggacaaaa gtcttttgaa tcgctccaca gttctcttgc
     6601 accagaacaa acaactttgg aaagccaatc acttattgaa actgaagtcc aaacttcata
     6661 ttactctatg ttaacaacaa tgaaaacata caatactaat gaggaagtgg aggaggaagg
     6721 cacgtccata gctcatatgt ctactccagg ccctggaatc aaaggcttgg aatcatatcc
     6781 tacccatcct gaagctactg ggaagtcata ctctttctca gcttctgcct tagtgactga
     6841 atctggacca gctagaagtg tagtcatgga ttcctcaact caagaggaag aaagtataaa
     6901 actcttccaa aaggacatga tactaacaca taaagagtca aattctgatc tctcgttctc
     6961 tggcctggga tcaggagagg ctttgcctcc tctccctaca acatcagtga gtttgactga
     7021 catgggaaag atcaatagca cattgtatcc tgagacatct cacatggaaa gcttaggaac
     7081 aagcatctta ggagataacc atgaaagaat gaaaaatgtg tcaaatgaag ttagaaccct
     7141 tatttccgaa acaggcagca tttctcaaga cagtacggag gcacccaata caactttgag
     7201 tgataccaga acagaagaat ctacaacttc acctcttcct ttcatgaagc tcatggacac
     7261 ggaacattct ccaaagcaga ccctcaggtg ggaagaggaa atccagaccc ataggccaca
     7321 aactatgact ggacagatga ccaatgacaa ttcttcagtg tcagaagctg aagcagcagc
     7381 aacctctgcc cctgctttcc tgcctgaaac ctacagtgtt gaaatgacca aagcctttgc
     7441 tacatcaccg tcacaaacat ctgacttgtt tgatgcaaat tctggagaag gatctggaga
     7501 agtggatggt cttgacttag tgtacacttc tagaactact caggcaagca gtcaaggaga
     7561 cagcatgttt gcctctcatg gattcattga aaagcatcca gaggtgtcaa ggaccgagac
     7621 tggagctact gatggatctc caacagcttc agcgatgttc ctgcatcagt cagagtacaa
     7681 tgagagttcc ctgtatccaa ccagcacact gccaagcaca gtgacatatg agagtccctc
     7741 agaaggtatt gcagatggtt tgcaagacca tatcagattc gaagtttcta ccttgaaacc
     7801 tagcagaagg aaagccactg agagcgttat tatagatcta gacaaagagg atagtaagga
     7861 cttagggttg gcaattactg agagtgctat tgttgaaatt ctccctgagc tgacatcaga
     7921 tagaaatatt attatagaca ttgatcacac taaacctgta tatgagtaca tccctggaat
     7981 acaaacagac ctagattcag acataccatt gggatcacat ggcagtagtg aagaaagcct
     8041 tgaagttcaa gagaagtatg aagcaactat taacctgtct ccaactgagg aagcatttga
     8101 cggctctggt gatgcacttc ctgctggtca cactcaagcg atatacaatg agtctgtgac
     8161 tcccagtgat ggaaagcagc cagaggacat aagctttagt tttgcaactg gaatcccagt
     8221 ttctagcaca gagacagaat taaatacttt cttccctaca gtgtcaaccc tgcacattcc
     8281 tagcaaacta accaccgcta gtccagagat tgataaaccc aacattgaag caatatcact
     8341 ggatgatata tttgaatcaa gcaccttgtc tgatggccaa gctattgccg atcagagtga
     8401 agtaatatca acactaggcc atttggaaaa gacacaggaa gagtatgaag aaaagaaata
     8461 tggaggtcct tcttttcagc cagaattctt ttcaggagtg ggggaagtat tcacagatgc
     8521 ccctgcttat gtaagtattg gtaggacata ctctgtagct cagcctttaa cagaattccc
     8581 caatgtggta ggacaatctg actccacaca ttacactgaa gcaacatctg cagtttcctc
     8641 tgtgacagag ctgtctcctc agacaccatc ttctccctct cctgtctaca tagacagtgg
     8701 agtctctgaa ttcacagagg tcccccacaa aagtgctcag ccagctccta ctgccgcttc
     8761 atcacaaaag ttgatagagg gttcttttaa gaaagtgcgt gctaatattg aagctaccat
     8821 caagtcgctg ggtgaaaatg atcacggaac agagtctcca tccatgtctc ctagccctgc
     8881 tttagacatt tcagaagacg acagtaaacc taagttacta gaagacctgg aaacttctcc
     8941 cacaaaaacc gagacttccc aagattcccc aaacaaagca aatgatcaaa ttccaggaaa
     9001 aacagctgga atccttgctg gtatcaaaac aacagagagt ggaccagtcg ttacagctgc
     9061 agatgacatg gagctaggag atgccacaca gcggccacat tctgcttctg ctcctgcagc
     9121 tttcagggtt gagacaagta tggtacctca gcccatccca caggagcctg agaggccaac
     9181 ctttccttcc ctggaaatta accatgaaac acacacatca ttatttgaag aatctatatt
     9241 ggcaacatct gagaaacaag tgtcccaaag aattcttgat tacagtaacc aggcaacagt
     9301 cagtactctg gatctaaata ctgaacactc aataccacca ttttccattc tggacaattc
     9361 taacgaaact gctttcctga ttggcattag tgaagagacc gtggaaggca cagcagttta
     9421 tctaccagga cctgatctct gcaaaacaaa cccatgcctc aatggaggca cctgctatcc
     9481 tactgagact tcctatgtgt gcacctgtgc acctggctac agtggagacc agtgtgaact
     9541 ggattttgat gaatgtcact ctaacccttg tcggaatgga gccacctgtg tggacggtct
     9601 gaatacattt agatgcctct gccttccgag ttatgtcggt gcactctgcg aacaagacac
     9661 tgagacatgc gactatggct ggcacaaatt ccaagggcaa tgctacaagt actttgctca
     9721 tcgccgtaca tgggatgctg ctgaaaggga gtgtcgcctg cagggtgccc acctcacaag
     9781 catcctttct catgaggaac aaatgtttgt gaatcgtgtg ggccatgatt accagtggat
     9841 tggcctcaat gacaagatgt ttgaacatga cttccgctgg actgacggca gcgcactgca
     9901 atatgagaac tggagaccca accagccaga cagcttcttt tctgctggag aagactgcgt
     9961 tgtgatcatt tggcatgaga atggccagtg gaatgacgtc ccctgcaact accacctcac
    10021 ctacacctgc aagaagggaa cagttgcttg cggccaaccc cctgttgtag aaaatgccaa
    10081 gacctttgga aagatgaaac cacgttatga aatcaactcc ttgattagat accactgcaa
    10141 agatggtttc attcagcgtc accttccaac tatccggtgc ctaggaaatg ggagatgggc
    10201 aatgcctaaa ataacctgca tgaacccatc tgcataccaa aggacttatt ctaagaaata
    10261 cttaaaaaat tcctcatcag tcaaggacaa ttctataaat acgtcaaaac atgagcatcg
    10321 ctggagccgg aggtggcagg aaacgaggcg ctgatcctaa aatggcgaac ataagcttca
    10381 ttcatcattt cagccaaagc cctgcctttc cgtgcctttc ctatcacctc aaggagaatt
    10441 agcagttggt ttggattttg ggactgccgt ctggtcattt ggggtggctg tattcctaaa
    10501 atattttcaa tgaaacatgg aattttgaaa aaaaaaagcg aataaaatga aagaaaatga
    10561 gcgaagaaga taaccatttt cagcctatct aatttcttta gttctctctc tgcctccagt
    10621 gcagactgtt tcctgtacac cagcctactg tactatttaa aaaacaaaac aaaacaaaac
    10681 gcaatatcag caccgatggc catgtaaata agatgattta atgttgattt taatcctgta
    10741 tataaaataa aacgtcatac ttggttcagg catatttaat gatgattacg gagctttcaa
    10801 ggtcttcgat cattggcttg gctgctttgc ttaggctgga aatggttttg tctacccttt
    10861 gactgtcagc aagtttgagg acggcttttc tggacaaccg gcaaacagaa tcttgcaggt
    10921 ctctgcacgc atgtcagcca cgggagtcac gctgatgcct ggctgataaa actctcggag
    10981 ctccagggtg taactcggct ttgccgtgtt ccttttcctt cacttacacg aagcgcctga
    11041 atggaggact tttctatgac ctgggacatt tttttagggg tcaaattgct aattattaac
    11101 tcaaccaggt ttcccttaca atggctttct taacactaac gctggtaagg tatttggaat
    11161 ggatatagac caagggttgt ggagggtggg ggaggagggt tttcttaaaa caacactcgt
    11221 gcaaaaaaat ttttttgtac atataaccat tttaatcctt tataaaaatt ttgaatgttc
    11281 atgtatgaat gctgcagctg cgaagcacac gtaaataaat gaagtaagcc atacggattt
    11341 aatttattgg atgttatttt tcccaagacc tgaaatcaat gtagtatgct agtaattttt
    11401 cagtgttagc ttttctattt tcttcacagt ttgatacctt ataatatagg tactcccttc
    11461 ccgtttcagt aatgtgacgg atagaacgca ccaagtgttt attacggaga atggaaacgt
    11521 gcttagctac ccataaatgg tgtttgccca ttccaagcaa gcgtgtaaat gtagaatagc
    11581 attgctcact gttaggtggc gtgtacgtgt taaagttttg cttctctctt cccagccatt
    11641 taccccttgc atttgaataa tgattgctga atcattatct agctatttta aggctcaagg
    11701 ttaaaactgc tcatggaaaa aggcatttaa ggctcatcca ttcccttcac ttacaacaac
    11761 ttacagaaaa attacgagta aaatgtgaat agaaaccagc ttcaatatga cacaatgaaa
    11821 acaagcacgg aaccatcgtg aggtgtccca taagtatggg cggactttat gttttatacc
    11881 aggtaaatac tcaacgaaga gtagaggaag agcagactgg ggttggtcct atgttgactg
    11941 tttctcacag aaccttcttt ggcttagagg gaatgactgt gctcacaagc atacgtagtg
    12001 tcttaggcga ggtctttgga ctaggcagta agttagtcaa tcctgatgtg ttctagggca
    12061 cagaaaatca ctgtgttgac ttactagggc tttttacttt ttcattacag tgcacataat
    12121 acttccctgt atttatatta taacatgtat agtgtaaaat gtgaatgact ttttcttttt
    12181 gtgaatgaaa atctaaaaaa atctttgtaa ctttttatat ctgcttttgt ttcaccaaag
    12241 aaacctaaaa attcttcttt tactaccttg aaaaaaaaaa a
//
