LOCUS       NM_031827                728 bp    mRNA    linear   ROD 01-APR-2024
DEFINITION  Rattus norvegicus vesicle-associated membrane protein 8 (Vamp8),
            mRNA.
ACCESSION   NM_031827
VERSION     NM_031827.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Messenger,S.W., Jones,E.K., Holthaus,C.L., Thomas,D.D.H.,
            Cooley,M.M., Byrne,J.A., Mareninova,O.A., Gukovskaya,A.S. and
            Groblewski,G.E.
  TITLE     Acute acinar pancreatitis blocks vesicle-associated membrane
            protein 8 (VAMP8)-dependent secretion, resulting in intracellular
            trypsin accumulation
  JOURNAL   J Biol Chem 292 (19), 7828-7839 (2017)
   PUBMED   28242757
  REMARK    GeneRIF: acute inhibition of VAMP8-mediated secretion during
            pancreatitis triggers intracellular trypsin accumulation and loss
            of the early endosomal compartment
REFERENCE   2  (bases 1 to 728)
  AUTHORS   Hubert,V., Peschel,A., Langer,B., Groger,M., Rees,A. and Kain,R.
  TITLE     LAMP-2 is required for incorporating syntaxin-17 into
            autophagosomes and for their fusion with lysosomes
  JOURNAL   Biol Open 5 (10), 1516-1529 (2016)
   PUBMED   27628032
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 728)
  AUTHORS   Malmersjo,S., Di Palma,S., Diao,J., Lai,Y., Pfuetzner,R.A.,
            Wang,A.L., McMahon,M.A., Hayer,A., Porteus,M., Bodenmiller,B.,
            Brunger,A.T. and Meyer,T.
  TITLE     Phosphorylation of residues inside the SNARE complex suppresses
            secretory vesicle fusion
  JOURNAL   EMBO J 35 (16), 1810-1821 (2016)
   PUBMED   27402227
  REMARK    GeneRIF: The authors took a proteomics approach using a PKCB
            knockout mast cell model and found that the key mast cell secretory
            protein VAMP8 becomes phosphorylated by PKC at multiple residues in
            the SNARE domain.
REFERENCE   4  (bases 1 to 728)
  AUTHORS   Diao,J., Liu,R., Rong,Y., Zhao,M., Zhang,J., Lai,Y., Zhou,Q.,
            Wilz,L.M., Li,J., Vivona,S., Pfuetzner,R.A., Brunger,A.T. and
            Zhong,Q.
  TITLE     ATG14 promotes membrane tethering and fusion of autophagosomes to
            endolysosomes
  JOURNAL   Nature 520 (7548), 563-566 (2015)
   PUBMED   25686604
REFERENCE   5  (bases 1 to 728)
  AUTHORS   Pirooz,S.D., He,S., Zhang,T., Zhang,X., Zhao,Z., Oh,S.,
            O'Connell,D., Khalilzadeh,P., Amini-Bavil-Olyaee,S., Farzan,M. and
            Liang,C.
  TITLE     UVRAG is required for virus entry through combinatorial interaction
            with the class C-Vps complex and SNAREs
  JOURNAL   Proc Natl Acad Sci U S A 111 (7), 2716-2721 (2014)
   PUBMED   24550300
REFERENCE   6  (bases 1 to 728)
  AUTHORS   Antonin,W., Holroyd,C., Fasshauer,D., Pabst,S., Von Mollard,G.F.
            and Jahn,R.
  TITLE     A SNARE complex mediating fusion of late endosomes defines
            conserved properties of SNARE structure and function
  JOURNAL   EMBO J 19 (23), 6453-6464 (2000)
   PUBMED   11101518
REFERENCE   7  (bases 1 to 728)
  AUTHORS   Antonin,W., Holroyd,C., Tikkanen,R., Honing,S. and Jahn,R.
  TITLE     The R-SNARE endobrevin/VAMP-8 mediates homotypic fusion of early
            endosomes and late endosomes
  JOURNAL   Mol Biol Cell 11 (10), 3289-3298 (2000)
   PUBMED   11029036
REFERENCE   8  (bases 1 to 728)
  AUTHORS   Verges,M., Havel,R.J. and Mostov,K.E.
  TITLE     A tubular endosomal fraction from rat liver: biochemical evidence
            of receptor sorting by default
  JOURNAL   Proc Natl Acad Sci U S A 96 (18), 10146-10151 (1999)
   PUBMED   10468577
REFERENCE   9  (bases 1 to 728)
  AUTHORS   Fasshauer,D., Antonin,W., Margittai,M., Pabst,S. and Jahn,R.
  TITLE     Mixed and non-cognate SNARE complexes. Characterization of assembly
            and biophysical properties
  JOURNAL   J Biol Chem 274 (22), 15440-15446 (1999)
   PUBMED   10336434
REFERENCE   10 (bases 1 to 728)
  AUTHORS   Wong,S.H., Zhang,T., Xu,Y., Subramaniam,V.N., Griffiths,G. and
            Hong,W.
  TITLE     Endobrevin, a novel synaptobrevin/VAMP-like protein preferentially
            associated with the early endosome
  JOURNAL   Mol Biol Cell 9 (6), 1549-1563 (1998)
   PUBMED   9614193
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000004.1.
            
            On Dec 3, 2020 this sequence version replaced NM_031827.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: CD372813.1, BG376981.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN16676784, SAMN16676785
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-141               JAXUCZ010000004.1  106005502-106005642 c
            142-297             JAXUCZ010000004.1  106003156-106003311 c
            298-728             JAXUCZ010000004.1  106000570-106001000 c
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="4"
                     /map="4q32"
     gene            1..728
                     /gene="Vamp8"
                     /note="vesicle-associated membrane protein 8"
                     /db_xref="GeneID:83730"
                     /db_xref="RGD:620421"
     exon            1..141
                     /gene="Vamp8"
                     /inference="alignment:Splign:2.1.0"
     CDS             139..441
                     /gene="Vamp8"
                     /note="EDB; VAMP-8; endobrevin"
                     /codon_start=1
                     /product="vesicle-associated membrane protein 8"
                     /protein_id="NP_114015.1"
                     /db_xref="GeneID:83730"
                     /db_xref="RGD:620421"
                     /translation="MEASGSAGNDRVRNLQSEVEGVKNIMTQNVERILARGENLDHLR
                     NKTEDLEATSEHFKTTSQKVARKFWWKNVKMIVIICVIVLIILILIILFATGTIPT"
     exon            142..297
                     /gene="Vamp8"
                     /inference="alignment:Splign:2.1.0"
     exon            298..728
                     /gene="Vamp8"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gagcccgaga ggctggagtc gcgggatggg caggaggcgg gcagaaagtt ggagtaagca
       61 ggaagtgaag ctgtagctgc cttgggtgga aacagactag gcgaagttct gctttgagag
      121 gcccaagtgc tccgagacat ggaggccagt gggagtgccg gaaatgaccg agtcaggaac
      181 ctgcagagtg aggtggaggg agtcaagaat attatgaccc agaatgtgga gcggatcttg
      241 gcccgagggg agaacctgga ccatctccga aacaagacag aggacttgga agccacgtct
      301 gaacacttca agacgacgtc ccagaaggtg gcccggaagt tctggtggaa gaatgtgaag
      361 atgattgtca tcatctgtgt gattgtcctt atcattctca tcctcatcat actctttgct
      421 acgggcacca tccccactta agagcccctc tcacctcacc ctactcttca ggggtgatcc
      481 tcccaaaagt gtgccaagag gggccctctc ctgccctcct ccccaggagt gctgctcggt
      541 ccctgtgagg gccttctgct gcctcatatg ccccagaagt tccttgatcc cctgggagga
      601 gacagctgga ctgtggctgc acctcatggt cctgagagtg ggttagagag acccagagga
      661 ccctgtttgt ggctgggaaa ctgctggtgg ttggtgggca ataaagacct cttggtatcc
      721 ttaagtgt
//
