LOCUS       NM_177930               3061 bp    mRNA    linear   ROD 30-MAR-2024
DEFINITION  Rattus norvegicus VAC14 component of PIKFYVE complex (Vac14), mRNA.
ACCESSION   NM_177930
VERSION     NM_177930.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Sbrissa,D., Ikonomov,O.C., Fu,Z., Ijuin,T., Gruenberg,J.,
            Takenawa,T. and Shisheva,A.
  TITLE     Core protein machinery for mammalian phosphatidylinositol
            3,5-bisphosphate synthesis and turnover that regulates the
            progression of endosomal transport. Novel Sac phosphatase joins the
            ArPIKfyve-PIKfyve complex
  JOURNAL   J Biol Chem 282 (33), 23878-23891 (2007)
   PUBMED   17556371
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000019.1.
            
            On Dec 2, 2020 this sequence version replaced NM_177930.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY220476.1, SRR26360186.435143.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-377               JAXUCZ010000019.1  55499962-55500338
            378-528             JAXUCZ010000019.1  55513894-55514044
            529-696             JAXUCZ010000019.1  55514313-55514480
            697-759             JAXUCZ010000019.1  55515327-55515389
            760-867             JAXUCZ010000019.1  55516147-55516254
            868-977             JAXUCZ010000019.1  55517104-55517213
            978-1084            JAXUCZ010000019.1  55517410-55517516
            1085-1219           JAXUCZ010000019.1  55518418-55518552
            1220-1372           JAXUCZ010000019.1  55518952-55519104
            1373-1436           JAXUCZ010000019.1  55527069-55527132
            1437-1581           JAXUCZ010000019.1  55533131-55533275
            1582-1647           JAXUCZ010000019.1  55533520-55533585
            1648-1804           JAXUCZ010000019.1  55550559-55550715
            1805-1937           JAXUCZ010000019.1  55562024-55562156
            1938-2112           JAXUCZ010000019.1  55591055-55591229
            2113-2231           JAXUCZ010000019.1  55592505-55592623
            2232-2311           JAXUCZ010000019.1  55593764-55593843
            2312-2462           JAXUCZ010000019.1  55596426-55596576
            2463-3061           JAXUCZ010000019.1  55600692-55601290
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="19"
                     /map="19q12"
     gene            1..3061
                     /gene="Vac14"
                     /note="VAC14 component of PIKFYVE complex"
                     /db_xref="GeneID:307842"
                     /db_xref="RGD:631410"
     exon            1..377
                     /gene="Vac14"
                     /inference="alignment:Splign:2.1.0"
     CDS             274..2625
                     /gene="Vac14"
                     /note="vacuole 14 protein; Vac14 homolog; Vac14, PIKFYVE
                     complex component"
                     /codon_start=1
                     /product="protein VAC14 homolog"
                     /protein_id="NP_808791.1"
                     /db_xref="GeneID:307842"
                     /db_xref="RGD:631410"
                     /translation="MNPEKDFAPLTPNIVRALNDKLYEKRKVAALEIEKLVRDFVAQN
                     NTVQIKHVIQTLSQEFALSQHPHSRKGGLIGLAACSIALGKDSGLYLKELIEPVLTCF
                     NDADSRLRYYACEALYNIVKVARGAVLPHFNVLFDGLSKLAADPDPNVKSGSELLDRL
                     LKDIVTESSKFDLVGFIPLLRERIYSNNQYARQFIISWILVLVSVPDINLLDYLPEIL
                     DGLFQILGDNGKEIRKMCEVVLGEFLKEIKKNPSSVKFAEMANILVIHCQTTDDLIQL
                     TAMCWMREFIQLAGRVMLPYSSGILTAVLPCLAYDDRKKSIKEVANVCNQSLMKLVTP
                     EDDEPDEPKSVAQKQTEPNPEDSLPKQEGTASGGASGPCDSSFGSGISVFTSASTDRA
                     PVTLHLDGIVQVLNCHLSDTTIGMMTRIAVLKWLYHLYIKTPRKMFRHTDSLFPILLQ
                     TLSDESDEVVLKDLEVLAEIASSPAGQTDDPGAPDGPDLQVNHSELQVPTSGRANLLN
                     PPNTKGLECSPSTPTMNSYFYKFMINLLQTFSSERKLLEARGPFIIRQLCLLLNAENI
                     FHSMADILLREEDLKFASTMVHTLNTILLTSTELFQLRNQLKDLKTLESQNLFCCLYR
                     SWCHNPVTTVSLCFLTQNYRHAYDLIQKFGDLEVTVDFLTEVDKLVQLIECPIFTYLR
                     LQLLDVKNNPYLMKALYGLLMLLPQSSAFQLLSHRLQCVPNPELLQTEDCLKAAPKSQ
                     KGDSPSIDYTELLQHFEKVQKQHLEVRHQRSGRGDHLDRRVVL"
     exon            378..528
                     /gene="Vac14"
                     /inference="alignment:Splign:2.1.0"
     exon            529..696
                     /gene="Vac14"
                     /inference="alignment:Splign:2.1.0"
     exon            697..759
                     /gene="Vac14"
                     /inference="alignment:Splign:2.1.0"
     exon            760..867
                     /gene="Vac14"
                     /inference="alignment:Splign:2.1.0"
     exon            868..977
                     /gene="Vac14"
                     /inference="alignment:Splign:2.1.0"
     exon            978..1084
                     /gene="Vac14"
                     /inference="alignment:Splign:2.1.0"
     exon            1085..1219
                     /gene="Vac14"
                     /inference="alignment:Splign:2.1.0"
     exon            1220..1372
                     /gene="Vac14"
                     /inference="alignment:Splign:2.1.0"
     exon            1373..1436
                     /gene="Vac14"
                     /inference="alignment:Splign:2.1.0"
     exon            1437..1581
                     /gene="Vac14"
                     /inference="alignment:Splign:2.1.0"
     exon            1582..1647
                     /gene="Vac14"
                     /inference="alignment:Splign:2.1.0"
     exon            1648..1804
                     /gene="Vac14"
                     /inference="alignment:Splign:2.1.0"
     exon            1805..1937
                     /gene="Vac14"
                     /inference="alignment:Splign:2.1.0"
     exon            1938..2112
                     /gene="Vac14"
                     /inference="alignment:Splign:2.1.0"
     exon            2113..2231
                     /gene="Vac14"
                     /inference="alignment:Splign:2.1.0"
     exon            2232..2311
                     /gene="Vac14"
                     /inference="alignment:Splign:2.1.0"
     exon            2312..2462
                     /gene="Vac14"
                     /inference="alignment:Splign:2.1.0"
     exon            2463..3061
                     /gene="Vac14"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggccgcggcc cagtgagcgt tgccggttcc tggcgggagc tggtcgcgtg agcggtgagg
       61 cggtccgggt cgcgcgtggg agaacagggt cggagcaggc agacccgagg cggcgcgtgg
      121 cggcaggacg aggcaggagg gccctgggcg gcgtagccca tgagcaggct cgggtgtccg
      181 cgcggagccc cacactcccc tgtccctggt acggcccggg cagcctcggg ctaaggtgtc
      241 tcgaggctcc cgggcgcagc gcgagctgcc accatgaacc cggagaagga ttttgcgcca
      301 ctcactccca acatcgtaag ggccctgaat gataaactct acgaaaagcg gaaggtagca
      361 gcgctggaga tcgagaagct ggtgagggac tttgtggccc agaacaacac tgtgcagatc
      421 aagcatgtga tccagaccct gtcgcaggag ttcgccctgt cccagcaccc ccacagccgg
      481 aaagggggtc tcatcggcct ggcagcctgc tctattgccc tcggcaagga ctcagggctc
      541 tacctgaagg agctgataga gccagtgttg acctgtttta atgatgcaga tagcaggctg
      601 cgctactatg cttgtgaggc cctctacaat atcgtcaaag tggcccgagg tgctgtgctg
      661 ccacacttca atgttctctt tgatggactg agtaagttgg ctgctgaccc agaccccaat
      721 gtgaaaagtg gatccgagct cctagaccgc cttttaaagg acattgtaac agaaagcagc
      781 aagtttgacc tggtgggctt catccccctt ttgcgggaga ggatttactc caacaaccaa
      841 tacgcccgac agttcatcat ctcctggatc ctggttctgg tgtcagtgcc agatattaac
      901 ctgctggatt acctgccaga gatcctggac gggctcttcc agatcctggg tgacaacggc
      961 aaagagattc ggaaaatgtg tgaggtggtt cttggagaat tcttgaaaga aattaagaag
     1021 aacccatcca gtgtgaagtt tgctgagatg gccaacatcc tggtgatcca ctgccagacc
     1081 acagatgacc tgattcagct gacggctatg tgctggatgc gggaattcat ccagctggca
     1141 ggccgggtaa tgctgcccta ttcctctggg atcctgactg ctgtcctgcc ctgcttggcc
     1201 tatgatgacc gcaagaaaag tatcaaggag gtggccaatg tgtgtaacca gagcctgatg
     1261 aagctggtta cccctgagga tgatgaacca gatgagccaa agtcagtagc acagaagcag
     1321 acagaaccca accctgagga ttccctgcca aagcaggagg ggacagccag tggaggtgca
     1381 agtggtccct gtgactccag ctttggaagt ggcatcagtg tctttacctc agccagcact
     1441 gacagggctc cagtgaccct gcatctggac ggaattgtgc aagtcctgaa ctgccacctt
     1501 agtgacacga ccattgggat gatgaccagg attgccgttc tgaagtggct ctaccatctc
     1561 tacatcaaaa ctccacgaaa gatgttccgg cacacagaca gtcttttccc catcctgctt
     1621 cagacgttat ctgatgaatc tgatgaggtt gtcttgaagg atctggaagt gttggcagaa
     1681 attgcttcct cccctgcagg ccagacggat gacccaggcg cacctgatgg ccctgacctc
     1741 caagtcaacc actcggagct tcaggtgccc acctccggca gagccaacct actaaaccct
     1801 cccaatacca aaggcttaga atgctctccc tccacgccca ccatgaactc ctacttttac
     1861 aagttcatga tcaaccttct gcagacattc agcagtgaac ggaagctcct ggaggccaga
     1921 ggcccattca tcatcagaca gctctgcctc ctgctgaatg cagagaacat cttccattcg
     1981 atggcagata tcctgctgcg ggaagaggac ctcaagtttg cgtccaccat ggtgcacacc
     2041 ctcaatacca tcctgctcac ctccactgag ctcttccagc tgagaaacca actcaaggac
     2101 ctgaagactc tggaaagcca gaacctcttc tgctgcctgt accgctcctg gtgccacaac
     2161 ccagtcacca ccgtatccct ctgcttcctc acccagaatt accgccatgc ctatgacctc
     2221 atccagaagt ttggggacct ggaagtcaca gtggatttcc tcacagaggt ggacaaactg
     2281 gtgcagctaa tcgagtgccc catctttaca tatctccgcc tgcagctgct ggacgtgaag
     2341 aacaacccgt acctgatgaa ggccctgtac ggcctgctca tgctgctgcc ccagagcagt
     2401 gccttccagc tgctctccca ccggctccag tgtgtgccca accctgagct gctgcagacc
     2461 gaagactgtc taaaagctgc ccccaagtcc cagaaaggtg actcgcccag catcgactac
     2521 acagagctgc tgcagcactt tgaaaaagtc cagaaacagc acctggaagt aagacaccag
     2581 cgcagtggac gtggggatca cctggaccgc agagttgtcc tctgatgtgc ctagcatgga
     2641 aaagggccct agtggcctaa gaagtactca gggtctctag gctgaggcca caaggagcct
     2701 gagaacctgc ctccagtagg gctggacctg accaccagtc cagggtaggg agggggacag
     2761 tcttggctct gtcagcccca gctcttccca gcagagcact tggaccccag cactgtcagc
     2821 tacaccctgg cttgggatcg atggcttctc tcctgatttg gaccacagcc ttaggatcca
     2881 gaagcagcct ctttctaatc cctgtctgtg agaagagctt gaccttgtcc ccggccccag
     2941 agcactgtca tggtgtgtat gcatatgcac atctgtgcag ataatgcaca caggccagca
     3001 caaagacctc tacagaatga tgttcacccc tgccccaata aagaaatgac agaatcctta
     3061 a
//
