LOCUS       NM_001106502            4065 bp    mRNA    linear   ROD 22-MAR-2023
DEFINITION  Rattus norvegicus ubiquitin specific peptidase 8 (Usp8), mRNA.
ACCESSION   NM_001106502 XM_001078286 XM_215821
VERSION     NM_001106502.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 4065)
  AUTHORS   Kerrisk Campbell M and Sheng M.
  TITLE     USP8 Deubiquitinates SHANK3 to Control Synapse Density and SHANK3
            Activity-Dependent Protein Levels
  JOURNAL   J Neurosci 38 (23), 5289-5301 (2018)
   PUBMED   29735556
  REMARK    GeneRIF: we identify USP8 as a key enzyme that regulates SHANK3
            protein levels in neurons
REFERENCE   2  (bases 1 to 4065)
  AUTHORS   Yeates EF and Tesco G.
  TITLE     The Endosome-associated Deubiquitinating Enzyme USP8 Regulates
            BACE1 Enzyme Ubiquitination and Degradation
  JOURNAL   J Biol Chem 291 (30), 15753-15766 (2016)
   PUBMED   27302062
REFERENCE   3  (bases 1 to 4065)
  AUTHORS   Durcan TM and Fon EA.
  TITLE     The three 'P's of mitophagy: PARKIN, PINK1, and post-translational
            modifications
  JOURNAL   Genes Dev 29 (10), 989-999 (2015)
   PUBMED   25995186
  REMARK    Review article
REFERENCE   4  (bases 1 to 4065)
  AUTHORS   Ceriani M, Amigoni L, D'Aloia A, Berruti G and Martegani E.
  TITLE     The deubiquitinating enzyme UBPy/USP8 interacts with TrkA and
            inhibits neuronal differentiation in PC12 cells
  JOURNAL   Exp Cell Res 333 (1), 49-59 (2015)
   PUBMED   25662281
  REMARK    GeneRIF: USP8 knockdown increases TrkA half-life, suggesting that
            the deubiquitinating activity of USP8 promotes TrkA degradation.
REFERENCE   5  (bases 1 to 4065)
  AUTHORS   Scudder SL, Goo MS, Cartier AE, Molteni A, Schwarz LA, Wright R and
            Patrick GN.
  TITLE     Synaptic strength is bidirectionally controlled by opposing
            activity-dependent regulation of Nedd4-1 and USP8
  JOURNAL   J Neurosci 34 (50), 16637-16649 (2014)
   PUBMED   25505317
  REMARK    GeneRIF: Homeostatic downscaling of synaptic strength is
            accompanied by an increase and decrease in Nedd4-1 and USP8 levels,
            respectively.
REFERENCE   6  (bases 1 to 4065)
  AUTHORS   Row PE, Prior IA, McCullough J, Clague MJ and Urbe S.
  TITLE     The ubiquitin isopeptidase UBPY regulates endosomal ubiquitin
            dynamics and is essential for receptor down-regulation
  JOURNAL   J Biol Chem 281 (18), 12618-12624 (2006)
   PUBMED   16520378
REFERENCE   7  (bases 1 to 4065)
  AUTHORS   Mizuno E, Iura T, Mukai A, Yoshimori T, Kitamura N and Komada M.
  TITLE     Regulation of epidermal growth factor receptor down-regulation by
            UBPY-mediated deubiquitination at endosomes
  JOURNAL   Mol Biol Cell 16 (11), 5163-5174 (2005)
   PUBMED   16120644
REFERENCE   8  (bases 1 to 4065)
  AUTHORS   Berruti G and Martegani E.
  TITLE     The deubiquitinating enzyme mUBPy interacts with the sperm-specific
            molecular chaperone MSJ-1: the relation with the proteasome,
            acrosome, and centrosome in mouse male germ cells
  JOURNAL   Biol Reprod 72 (1), 14-21 (2005)
   PUBMED   15342353
REFERENCE   9  (bases 1 to 4065)
  AUTHORS   Wu X, Yen L, Irwin L, Sweeney C and Carraway KL 3rd.
  TITLE     Stabilization of the E3 ubiquitin ligase Nrdp1 by the
            deubiquitinating enzyme USP8
  JOURNAL   Mol Cell Biol 24 (17), 7748-7757 (2004)
   PUBMED   15314180
REFERENCE   10 (bases 1 to 4065)
  AUTHORS   Gnesutta N, Ceriani M, Innocenti M, Mauri I, Zippel R, Sturani E,
            Borgonovo B, Berruti G and Martegani E.
  TITLE     Cloning and characterization of mouse UBPy, a deubiquitinating
            enzyme that interacts with the ras guanine nucleotide exchange
            factor CDC25(Mm)/Ras-GRF1
  JOURNAL   J Biol Chem 276 (42), 39448-39454 (2001)
   PUBMED   11500497
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from CH473949.1.
            
            [WARNING] On May 3, 2024 this sequence was replaced by
            NM_001106502.2.
            
            On or before Oct 4, 2007 this sequence version replaced
            XM_215821.4, XM_001078286.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..4065
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="3"
                     /map="3q36"
     gene            1..4065
                     /gene="Usp8"
                     /note="ubiquitin specific peptidase 8"
                     /db_xref="GeneID:296121"
                     /db_xref="RGD:1304979"
     exon            1..139
                     /gene="Usp8"
                     /inference="alignment:Splign:2.1.0"
     exon            140..258
                     /gene="Usp8"
                     /inference="alignment:Splign:2.1.0"
     CDS             155..3400
                     /gene="Usp8"
                     /EC_number="3.4.19.12"
                     /note="ubiquitin specific protease 8"
                     /codon_start=1
                     /product="ubiquitin carboxyl-terminal hydrolase 8"
                     /protein_id="NP_001099972.1"
                     /db_xref="GeneID:296121"
                     /db_xref="RGD:1304979"
                     /translation="MPAVASVPKELYLSSSLKDLNKKTEVKPEKTSTKNYIHSAQKIF
                     KAAEECRLDRDEERAYVLYMKYVAVYNLIKKRPDFKQQQDYYLSILGPANIKKAIEEA
                     ERLSESLKLRYEEAEVRKQLEEKDRREEEQLQQQKRQEMGREDSGAAAKRSVENLLDS
                     KTKIQRVNGEKSEGGAAAERGAVTAKELYTMMMDKNTSLIIMDARKIQDYQHSCILGS
                     LSVPEEAISPGVTANWIEAKLSDDSKDTWKKRGSVDYVVLLDWFSSAKDLLLGTTLRS
                     LKDALFKWESKAVLHHEPLVLEGGYENWLLCYPQFTTNAKVTPPPRSRPEEVSVSLDF
                     TYPSLEEPVPSKLPAQMPPPPLEANEKAQLVTDQDGKPRPLVQSALAGPSVAPKAEAS
                     PIIQPVPATKNVPQVDRTKKPAVKLPEDHRMKSESTDQSGRVLSDRSTKPVFTSPATM
                     LTDEEKARIHEETALLMEKNRQEKELWERQQKEQKEKLRREEQERKAGKTQDAEERDF
                     TENQHKAKDGQEKRDSKQTKAEDREPPADGAQDATGTQRQSKSEHDASDAKVSVEGKR
                     CPMSEVQKRPADVSSASSMSGELSAGKAQREPLTRARSEEMGRIVPGLPLGWAKFLDP
                     ITGTFRYYHSPTNTVHMYPPEMAPSSVPPSTPPTHKVKPQIPAERDREPSKLKRSYSS
                     PDITQALQEEEKRRPAVTPTVNRENKPPCYPKAEISRLSASQIRNLNPVFGGSGPALT
                     GLRNLGNTCYMNSILQCLCNAPHLADYFNRNCYQDDINRSNLLGHKGEVAEEFGIIMK
                     ALWTGQYRYISPKDFKVTIGKINDQFAGSSQQDSQELLLFLMDGLHEDLNKADNRKRH
                     KEENNDHLDDFKAAEHAWQKHKQLNESIIVALFQGQFKSTVQCLTCHKKSRTFEAFMY
                     LSLPLASTSKCTLQDCLRLFSKEEKLTDNNRFYCSHCRARRDSLKKIEIWKLPPVLLV
                     HLKRFSYDGRWKQKLQTSVDFPLENLDLSQYVIGPKSSLKKYNLFSVSNHYGGLDGGH
                     YTAYCKNAARQRWFKFDDHEVSDISVSSVRSSAAYILFYTSLGPRVTEAAT"
     exon            259..403
                     /gene="Usp8"
                     /inference="alignment:Splign:2.1.0"
     exon            404..489
                     /gene="Usp8"
                     /inference="alignment:Splign:2.1.0"
     exon            490..652
                     /gene="Usp8"
                     /inference="alignment:Splign:2.1.0"
     exon            653..695
                     /gene="Usp8"
                     /inference="alignment:Splign:2.1.0"
     exon            696..840
                     /gene="Usp8"
                     /inference="alignment:Splign:2.1.0"
     exon            841..1003
                     /gene="Usp8"
                     /inference="alignment:Splign:2.1.0"
     exon            1004..1148
                     /gene="Usp8"
                     /inference="alignment:Splign:2.1.0"
     exon            1149..1372
                     /gene="Usp8"
                     /inference="alignment:Splign:2.1.0"
     exon            1373..1933
                     /gene="Usp8"
                     /inference="alignment:Splign:2.1.0"
     exon            1934..2014
                     /gene="Usp8"
                     /inference="alignment:Splign:2.1.0"
     exon            2015..2277
                     /gene="Usp8"
                     /inference="alignment:Splign:2.1.0"
     exon            2278..2490
                     /gene="Usp8"
                     /inference="alignment:Splign:2.1.0"
     exon            2491..2701
                     /gene="Usp8"
                     /inference="alignment:Splign:2.1.0"
     exon            2702..2938
                     /gene="Usp8"
                     /inference="alignment:Splign:2.1.0"
     exon            2939..3081
                     /gene="Usp8"
                     /inference="alignment:Splign:2.1.0"
     exon            3082..3214
                     /gene="Usp8"
                     /inference="alignment:Splign:2.1.0"
     exon            3215..4065
                     /gene="Usp8"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ccgtcgcacg caatgcgaat cgggaagagg ggatgatctg cgctgctttg cgtctgagaa
       61 cggaggctcg ttctccttct ggtggtgggc gatgggcgtc aggcgagaag gccggcgtta
      121 gcggagcgcg ggcgctgcgc aagaatcatt catcatgcct gctgtagctt cagttcctaa
      181 agaactctac ctcagttctt cactcaaaga cctcaataag aagacagaag ttaaacctga
      241 gaaaaccagc accaagaatt atatacacag cgctcagaag attttcaagg cagcagaaga
      301 atgcagacta gatcgtgatg aggaaagggc ctatgtgctt tatatgaaat atgtggcggt
      361 ttataatctt atcaaaaaga gacctgattt caagcaacag caggactatt acctttcaat
      421 acttggacct gcaaacatca aaaaggctat tgaagaagct gaaagactct ctgagagcct
      481 caaactgaga tacgaagagg ctgaagttcg gaaacagctt gaagaaaagg acagacggga
      541 ggaagaacag ctgcagcaac agaaaaggca ggagatggga agagaggata gtggtgcggc
      601 ggccaaacgc tccgtggaaa atttactgga ttccaaaacc aaaatccaaa gggtcaatgg
      661 agagaagagc gaaggaggtg cagcagcaga gagaggagca gtcacagcaa aggagctata
      721 tacaatgatg atggataaaa acacaagcct gattataatg gatgctcgga aaatacagga
      781 ttatcagcat tcctgtatct tgggctctct cagtgttcct gaagaagcta tcagtccagg
      841 agtcactgcc aattggattg aagcaaagct ttcagatgat tctaaagaca cgtggaaaaa
      901 aagggggagt gtggactatg tggtcctgct ggactggttt agttcagcga aagatttgct
      961 gctcgggacc acgctacgga gtctgaaaga tgctcttttc aagtgggaaa gtaaagctgt
     1021 cctgcaccat gagcccttgg tgctggaggg cggctatgag aactggctcc tgtgctatcc
     1081 acagttcaca acaaatgcta aggttactcc accccctcgg agcaggcctg aagaggtgtc
     1141 tgtctcattg gattttactt atccctcact ggaagaacca gttccttcca aacttcctgc
     1201 ccagatgcca cctcctcctt tagaagcaaa tgagaaggca cagttggtaa ctgatcaaga
     1261 tggtaaaccg agaccgttag tccagtcggc tctagccgga cccagtgtgg ctcccaaagc
     1321 tgaagcttca cccataattc agccagtgcc tgctacaaag aatgttccac aggttgatcg
     1381 tacaaaaaaa ccagcagtta agttgcctga agatcataga atgaaatctg aaagtacaga
     1441 tcagagtgga agagttcttt ctgaccgatc caccaagcca gtatttacct ctccagccac
     1501 catgctaaca gatgaagaaa aggctcgcat tcatgaagaa acagcccttc ttatggaaaa
     1561 gaatagacag gagaaggaac tgtgggaaag gcagcagaag gaacagaaag agaagctgag
     1621 aagggaggaa caagagcgca aagctggaaa gacccaggat gcagaagaac gggacttcac
     1681 tgagaatcag cacaaagcaa aggatggaca agagaagaga gacagcaaac agaccaaggc
     1741 agaagacaga gagcccccag cagatggggc ccaggacgcc acaggaacgc aaagacaaag
     1801 taagagtgaa cacgatgctt ccgatgctaa ggtatctgtg gaaggtaaaa ggtgtcccat
     1861 gtcagaagtg cagaagaggc cagcagatgt gtcgtctgca tcctccatgt cgggagagct
     1921 gagtgcaggc aaggctcagc gagaaccttt gacaagagca agaagtgaag aaatggggag
     1981 aattgtgcca ggactgcctt taggctgggc caagtttctt gatccaatca ctgggacttt
     2041 tcgttactac cattcaccca caaacacggt tcacatgtat ccgcctgaga tggctccttc
     2101 gtctgtgcct ccttccaccc ctcccactca caaagtcaag ccccagatcc ctgctgagcg
     2161 ggacagggag ccatccaaac tgaagcgctc ctactcctca ccagacatca ctcaggccct
     2221 gcaggaggag gagaagagga ggccagcagt gaccccgaca gtcaaccggg agaacaagcc
     2281 gccgtgttac cctaaagctg agatctcgag gctttctgct tctcagattc ggaacctcaa
     2341 tcccgttttt ggaggatctg gaccagctct tactggactt cgtaatttgg gaaatacttg
     2401 ttacatgaac tcaatattac agtgcctgtg caatgctcca catttggctg attatttcaa
     2461 ccgaaactgc taccaggacg atatcaacag gtcaaatttg ttggggcata aaggtgaagt
     2521 ggcagaagaa tttggtataa tcatgaaggc actgtggaca ggacagtata gatacatcag
     2581 tccaaaagac tttaaagtca ccattggtaa gattaatgac cagtttgcag gatccagcca
     2641 gcaagattca caagagctgc tgcttttcct catggatggc ctccacgagg atctaaataa
     2701 ggctgacaat cggaagaggc ataaggaaga gaacaatgac cacctcgatg actttaaagc
     2761 ggcagaacat gcctggcaga agcacaagca gctcaatgag tccattattg tcgcactctt
     2821 tcagggtcag ttcaaatcca cagtgcagtg cctcacctgt cacaagaagt ctcggacatt
     2881 cgaggctttc atgtatttgt ctttgccact agcatccaca agtaaatgta ctttacagga
     2941 ctgccttaga ttattttcca aagaagaaaa gcttacagat aacaacagat tttactgcag
     3001 ccattgccga gctcggcggg attctctaaa gaaaatagaa atctggaaat tgcctcctgt
     3061 gctgttagta cacctgaaac gattctccta tgacggcagg tggaagcaga aactgcaaac
     3121 gtccgtggat ttcccattag aaaatcttga cctgtcacag tatgttattg gcccaaaaag
     3181 cagtttgaag aaatataact tgttttctgt ttcaaaccac tacggcgggc tcgatggagg
     3241 ccactacacg gcctattgta aaaacgcggc gaggcagcgc tggtttaagt ttgatgacca
     3301 cgaggtttcg gacatctccg tgtcttctgt gaggtcgtca gcagcttata tcctgtttta
     3361 cacctccctg gggccacgcg tgactgaggc agccacataa caaggcgtag ggtatggggt
     3421 gacatttcta cattgcagca gcacatctaa tgcttagaga tcatggctcg ctgcagccgc
     3481 tatgcagagg agcccagaac actgggggct gtgctactag caccatagaa ttcaggtcag
     3541 tgctgtttat caaaaaagaa agaaaaaacc ctgacattta acaactatca tagtgcacat
     3601 tccttacagg aatcatttat ttcaaaacaa ctttttgagt ctgccccaaa gttaaataat
     3661 tcattagcca gacatttatt attctattgg taccaaacca ttgtaacctc cattttccta
     3721 tccactgctc tagcctcttc ctacttctgt cccagctcga cccactgaac tctctagaat
     3781 ggcctaaccc agataggaat gtatgtgtac atatttactg cactgctgca catcagacca
     3841 ctgtacatac ttaacagatc ctctgtggac gcctagaaag catccgtcct ttttcttccc
     3901 acctcttcta ggtagctgca atgcttcctt agggaaatga cagggcaaaa ttatttttct
     3961 gttggcttcc ggctgtattt gtgcactaaa tctttattct aaaaaatgga aactttaatt
     4021 ttttaaaaat aattcattta catctacatt aaaatcttaa cgagg
//
