LOCUS       NM_022861               7985 bp    mRNA    linear   ROD 19-AUG-2024
DEFINITION  Rattus norvegicus unc-13 homolog A (Unc13a), mRNA.
ACCESSION   NM_022861
VERSION     NM_022861.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 7985)
  AUTHORS   Bhaskar,B.R., Yadav,L., Sriram,M., Sanghrajka,K., Gupta,M., V,B.K.,
            Nellikka,R.K. and Das,D.
  TITLE     Differential SNARE chaperoning by Munc13-1 and Munc18-1 dictates
            fusion pore fate at the release site
  JOURNAL   Nat Commun 15 (1), 4132 (2024)
   PUBMED   38755165
  REMARK    GeneRIF: Differential SNARE chaperoning by Munc13-1 and Munc18-1
            dictates fusion pore fate at the release site.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7985)
  AUTHORS   Li,F., Kalyana Sundaram,R.V., Gatta,A.T., Coleman,J.,
            Ramakrishnan,S., Krishnakumar,S.S., Pincet,F. and Rothman,J.E.
  TITLE     Vesicle capture by membrane-bound Munc13-1 requires self-assembly
            into discrete clusters
  JOURNAL   FEBS Lett 595 (17), 2185-2196 (2021)
   PUBMED   34227103
  REMARK    GeneRIF: Vesicle capture by membrane-bound Munc13-1 requires
            self-assembly into discrete clusters.
REFERENCE   3  (bases 1 to 7985)
  AUTHORS   Stepien,K.P. and Rizo,J.
  TITLE     Synaptotagmin-1-, Munc18-1-, and Munc13-1-dependent liposome fusion
            with a few neuronal SNAREs
  JOURNAL   Proc Natl Acad Sci U S A 118 (4) (2021)
   PUBMED   33468652
  REMARK    GeneRIF: Synaptotagmin-1-, Munc18-1-, and Munc13-1-dependent
            liposome fusion with a few neuronal SNAREs.
REFERENCE   4  (bases 1 to 7985)
  AUTHORS   Wang,X., Gong,J., Zhu,L., Wang,S., Yang,X., Xu,Y., Yang,X. and
            Ma,C.
  TITLE     Munc13 activates the Munc18-1/syntaxin-1 complex and enables
            Munc18-1 to prime SNARE assembly
  JOURNAL   EMBO J 39 (16), e103631 (2020)
   PUBMED   32643828
  REMARK    GeneRIF: Munc13 activates the Munc18-1/syntaxin-1 complex and
            enables Munc18-1 to prime SNARE assembly.
REFERENCE   5  (bases 1 to 7985)
  AUTHORS   Magdziarek,M., Bolembach,A.A., Stepien,K.P., Quade,B., Liu,X. and
            Rizo,J.
  TITLE     Re-examining how Munc13-1 facilitates opening of syntaxin-1
  JOURNAL   Protein Sci 29 (6), 1440-1458 (2020)
   PUBMED   32086964
  REMARK    GeneRIF: Re-examining how Munc13-1 facilitates opening of
            syntaxin-1.
REFERENCE   6  (bases 1 to 7985)
  AUTHORS   Sakaguchi,G., Orita,S., Naito,A., Maeda,M., Igarashi,H., Sasaki,T.
            and Takai,Y.
  TITLE     A novel brain-specific isoform of beta spectrin: isolation and its
            interaction with Munc13
  JOURNAL   Biochem Biophys Res Commun 248 (3), 846-851 (1998)
   PUBMED   9704016
REFERENCE   7  (bases 1 to 7985)
  AUTHORS   Betz,A., Ashery,U., Rickmann,M., Augustin,I., Neher,E.,
            Sudhof,T.C., Rettig,J. and Brose,N.
  TITLE     Munc13-1 is a presynaptic phorbol ester receptor that enhances
            neurotransmitter release
  JOURNAL   Neuron 21 (1), 123-136 (1998)
   PUBMED   9697857
REFERENCE   8  (bases 1 to 7985)
  AUTHORS   Orita,S., Naito,A., Sakaguchi,G., Maeda,M., Igarashi,H., Sasaki,T.
            and Takai,Y.
  TITLE     Physical and functional interactions of Doc2 and Munc13 in
            Ca2+-dependent exocytotic machinery
  JOURNAL   J Biol Chem 272 (26), 16081-16084 (1997)
   PUBMED   9195900
REFERENCE   9  (bases 1 to 7985)
  AUTHORS   Betz,A., Okamoto,M., Benseler,F. and Brose,N.
  TITLE     Direct interaction of the rat unc-13 homologue Munc13-1 with the N
            terminus of syntaxin
  JOURNAL   J Biol Chem 272 (4), 2520-2526 (1997)
   PUBMED   8999968
REFERENCE   10 (bases 1 to 7985)
  AUTHORS   Brose,N., Hofmann,K., Hata,Y. and Sudhof,T.C.
  TITLE     Mammalian homologues of Caenorhabditis elegans unc-13 gene define
            novel family of C2-domain proteins
  JOURNAL   J Biol Chem 270 (42), 25273-25280 (1995)
   PUBMED   7559667
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000016.1.
            
            On Mar 8, 2021 this sequence version replaced NM_022861.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U24070.1 [ECO:0000332]
            RNAseq introns              :: partial sample support SAMEA5760393,
                                           SAMEA5760433 [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-113               JAXUCZ010000016.1  18415674-18415786   c
            114-143             JAXUCZ010000016.1  18409940-18409969   c
            144-243             JAXUCZ010000016.1  18409280-18409379   c
            244-361             JAXUCZ010000016.1  18407254-18407371   c
            362-485             JAXUCZ010000016.1  18406343-18406466   c
            486-559             JAXUCZ010000016.1  18406032-18406105   c
            560-614             JAXUCZ010000016.1  18405652-18405706   c
            615-647             JAXUCZ010000016.1  18404999-18405031   c
            648-855             JAXUCZ010000016.1  18403663-18403870   c
            856-1413            JAXUCZ010000016.1  18400358-18400915   c
            1414-1522           JAXUCZ010000016.1  18400105-18400213   c
            1523-1569           JAXUCZ010000016.1  18398356-18398402   c
            1570-1648           JAXUCZ010000016.1  18397755-18397833   c
            1649-1654           JAXUCZ010000016.1  18397602-18397607   c
            1655-1726           JAXUCZ010000016.1  18397263-18397334   c
            1727-1946           JAXUCZ010000016.1  18396939-18397158   c
            1947-2174           JAXUCZ010000016.1  18395486-18395713   c
            2175-2316           JAXUCZ010000016.1  18394748-18394889   c
            2317-2486           JAXUCZ010000016.1  18394454-18394623   c
            2487-2602           JAXUCZ010000016.1  18394183-18394298   c
            2603-2766           JAXUCZ010000016.1  18393205-18393368   c
            2767-2917           JAXUCZ010000016.1  18392498-18392648   c
            2918-2986           JAXUCZ010000016.1  18391821-18391889   c
            2987-3076           JAXUCZ010000016.1  18391553-18391642   c
            3077-3211           JAXUCZ010000016.1  18390831-18390965   c
            3212-3345           JAXUCZ010000016.1  18389717-18389850   c
            3346-3431           JAXUCZ010000016.1  18385346-18385431   c
            3432-3558           JAXUCZ010000016.1  18385135-18385261   c
            3559-3655           JAXUCZ010000016.1  18384369-18384465   c
            3656-3799           JAXUCZ010000016.1  18384031-18384174   c
            3800-3883           JAXUCZ010000016.1  18382893-18382976   c
            3884-3961           JAXUCZ010000016.1  18381927-18382004   c
            3962-4050           JAXUCZ010000016.1  18381649-18381737   c
            4051-4203           JAXUCZ010000016.1  18381141-18381293   c
            4204-4327           JAXUCZ010000016.1  18380405-18380528   c
            4328-4333           JAXUCZ010000016.1  18379950-18379955   c
            4334-4372           JAXUCZ010000016.1  18378488-18378526   c
            4373-4402           JAXUCZ010000016.1  18377274-18377303   c
            4403-4459           JAXUCZ010000016.1  18375936-18375992   c
            4460-4540           JAXUCZ010000016.1  18375642-18375722   c
            4541-4688           JAXUCZ010000016.1  18375255-18375402   c
            4689-4745           JAXUCZ010000016.1  18374211-18374267   c
            4746-4838           JAXUCZ010000016.1  18371938-18372030   c
            4839-4998           JAXUCZ010000016.1  18371501-18371660   c
            4999-7985           JAXUCZ010000016.1  18367891-18370877   c
FEATURES             Location/Qualifiers
     source          1..7985
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="16"
                     /map="16p14"
     gene            1..7985
                     /gene="Unc13a"
                     /gene_synonym="Munc13-1; Unc13h1"
                     /note="unc-13 homolog A"
                     /db_xref="GeneID:64829"
                     /db_xref="RGD:619722"
     exon            1..113
                     /gene="Unc13a"
                     /gene_synonym="Munc13-1; Unc13h1"
                     /inference="alignment:Splign:2.1.0"
     CDS             92..5299
                     /gene="Unc13a"
                     /gene_synonym="Munc13-1; Unc13h1"
                     /codon_start=1
                     /product="protein unc-13 homolog A"
                     /protein_id="NP_074052.2"
                     /db_xref="GeneID:64829"
                     /db_xref="RGD:619722"
                     /translation="MSLLCVGVKKAKFDGAQEKFNTYVTLKVQNVKSTTIAVRGSQPS
                     WEQDFMFEINRLDLGLTVEVWNKGLIWDTMVGTVWIPLRTIRQSNEEGPGEWLTLDSQ
                     AIMADSEICGTKDPTFHRILLDAHFELPLDIPEEEARYWAKKLEQLNAMRDQDEYSFQ
                     DQQDKPLPVPSSQCCNWNYFGWGEQNDDPDSAVDDRDSDYRSETSNSIPPPYYTTSQP
                     NASVHQYSVRPPPLGSRESYSDSMHSYEEFSEPRALSPTGSSRYASSGELSQGSSQLS
                     EDFDPDEHSLQGSELDDERDRDSYHSCHSSVSYHKDSPRWDQDEEDLEDLEDLEDEEL
                     PEEEELEEEELEEEEELEEEELELEEEEEVPDDLASYTQQEDTTVAEPKEFKRISFPT
                     AAPQKEDKVSAVPIEAPDVSKGIPKAATPEEKAAAECAQEAEPPKSEESFRSREAEEG
                     QEGQDAMSRAKANWLRAFNKVRMQLQEARGEGEMSKSLWFKGGPGGGLIIIDSMPDIR
                     KRKPIPLVSDLAMSLVQSRKAGITSALASSTLNNEELKNHVYKKTLQALIYPISCTTP
                     HNFEVWTATTPTYCYECEGLLWGIARQGMRCTECGVKCHEKCQDLLNADCLQRAAEKS
                     SKHGAEDRTQNIIMVLKDRMKIRERNKPEIFELIQEVFAVTKSAHTQQMKAVKQSVLD
                     GTSKWSAKISITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIYGNLNPVWEENF
                     HFECHNSSDRIKVRVWDEDDDIKSRVKQRFKRESDDFLGQTIIEVRTLSGEMDVWYNL
                     DKRTDKSAVSGAIRLHISVEIKGEEKVAPYHVQYTCLHENLFHFVTDVQNNGVVKIPD
                     AKGDDAWKVYYDETAQEIVDEFAMRYGVESIYQAMTHFACLSSKYMCPGVPAVMSTLL
                     ANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSMYRNNFPASS
                     PERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHE
                     LYGREYQTDPAKKGEVPPEEQGPSIKNLDFWSKLITLIVSIIEEDKNSYTPCLNQFPQ
                     ELNVGKISAEVMWSLFAQDMKYAMEEHDKHRLCKSADYMNLHFKVKWLYNEYVAELPT
                     FKDRVPEYPAWFEPFVIQWLDENEEVSRDFLHGALERDKKDGFQQTSEHALFSCSVVD
                     VFSQLNQSFEIIKKLECPDPQIVGHYMRRFAKTISNVLLQYADIVSKDFASYCSKEKE
                     KVPCILMNNTQQLRVQLEKMFEAMGGKELDAEASGTLKELQVKLNNVLDELSHVFATS
                     FQPHIEECVRQMGDILSQVKGTGNVPASACSSVAQDADNVLQPIMDLLDSNLTLFAKI
                     CEKTVLKRVLKELWKLVMNTMERTIVLPPLTDQTMIGTLLRKHGKGLEKGRVKLPSHS
                     DGTQMIFNAAKELGQLSKLKDHMVREEAKSLTPKQCAVVELALDTIKQYFHAGGVGLK
                     KTFLEKSPDLQSLRYALSLYTQATDLLIKTFVQTQSAQVHGGKGTRFTLSEDVCPEMG
                     SGVEDPVGEVSVHVELFTHPGTGEQKVTVKVVAANDLKWQTSGIFRPFIEVNIVGPQL
                     SDKKRKFATKSKNNSWAPKYNESFQFSLSADAGPECYELQVCVKDYCFAREDRTVGLA
                     VLQLRELAQRGSAACWLPLGRRIHMDDTGLTVLRILSQRSNDEVAKEFVKLKSDTRSA
                     EEGGAAPAP"
     exon            114..143
                     /gene="Unc13a"
                     /gene_synonym="Munc13-1; Unc13h1"
                     /inference="alignment:Splign:2.1.0"
     exon            144..243
                     /gene="Unc13a"
                     /gene_synonym="Munc13-1; Unc13h1"
                     /inference="alignment:Splign:2.1.0"
     exon            244..361
                     /gene="Unc13a"
                     /gene_synonym="Munc13-1; Unc13h1"
                     /inference="alignment:Splign:2.1.0"
     exon            362..485
                     /gene="Unc13a"
                     /gene_synonym="Munc13-1; Unc13h1"
                     /inference="alignment:Splign:2.1.0"
     exon            486..559
                     /gene="Unc13a"
                     /gene_synonym="Munc13-1; Unc13h1"
                     /inference="alignment:Splign:2.1.0"
     exon            560..614
                     /gene="Unc13a"
                     /gene_synonym="Munc13-1; Unc13h1"
                     /inference="alignment:Splign:2.1.0"
     exon            615..647
                     /gene="Unc13a"
                     /gene_synonym="Munc13-1; Unc13h1"
                     /inference="alignment:Splign:2.1.0"
     exon            648..855
                     /gene="Unc13a"
                     /gene_synonym="Munc13-1; Unc13h1"
                     /inference="alignment:Splign:2.1.0"
     exon            856..1413
                     /gene="Unc13a"
                     /gene_synonym="Munc13-1; Unc13h1"
                     /inference="alignment:Splign:2.1.0"
     exon            1414..1522
                     /gene="Unc13a"
                     /gene_synonym="Munc13-1; Unc13h1"
                     /inference="alignment:Splign:2.1.0"
     exon            1523..1569
                     /gene="Unc13a"
                     /gene_synonym="Munc13-1; Unc13h1"
                     /inference="alignment:Splign:2.1.0"
     exon            1570..1648
                     /gene="Unc13a"
                     /gene_synonym="Munc13-1; Unc13h1"
                     /inference="alignment:Splign:2.1.0"
     exon            1649..1654
                     /gene="Unc13a"
                     /gene_synonym="Munc13-1; Unc13h1"
                     /inference="alignment:Splign:2.1.0"
     exon            1655..1726
                     /gene="Unc13a"
                     /gene_synonym="Munc13-1; Unc13h1"
                     /inference="alignment:Splign:2.1.0"
     exon            1727..1946
                     /gene="Unc13a"
                     /gene_synonym="Munc13-1; Unc13h1"
                     /inference="alignment:Splign:2.1.0"
     exon            1947..2174
                     /gene="Unc13a"
                     /gene_synonym="Munc13-1; Unc13h1"
                     /inference="alignment:Splign:2.1.0"
     exon            2175..2316
                     /gene="Unc13a"
                     /gene_synonym="Munc13-1; Unc13h1"
                     /inference="alignment:Splign:2.1.0"
     exon            2317..2486
                     /gene="Unc13a"
                     /gene_synonym="Munc13-1; Unc13h1"
                     /inference="alignment:Splign:2.1.0"
     exon            2487..2602
                     /gene="Unc13a"
                     /gene_synonym="Munc13-1; Unc13h1"
                     /inference="alignment:Splign:2.1.0"
     exon            2603..2766
                     /gene="Unc13a"
                     /gene_synonym="Munc13-1; Unc13h1"
                     /inference="alignment:Splign:2.1.0"
     exon            2767..2917
                     /gene="Unc13a"
                     /gene_synonym="Munc13-1; Unc13h1"
                     /inference="alignment:Splign:2.1.0"
     exon            2918..2986
                     /gene="Unc13a"
                     /gene_synonym="Munc13-1; Unc13h1"
                     /inference="alignment:Splign:2.1.0"
     exon            2987..3076
                     /gene="Unc13a"
                     /gene_synonym="Munc13-1; Unc13h1"
                     /inference="alignment:Splign:2.1.0"
     exon            3077..3211
                     /gene="Unc13a"
                     /gene_synonym="Munc13-1; Unc13h1"
                     /inference="alignment:Splign:2.1.0"
     exon            3212..3345
                     /gene="Unc13a"
                     /gene_synonym="Munc13-1; Unc13h1"
                     /inference="alignment:Splign:2.1.0"
     exon            3346..3431
                     /gene="Unc13a"
                     /gene_synonym="Munc13-1; Unc13h1"
                     /inference="alignment:Splign:2.1.0"
     exon            3432..3558
                     /gene="Unc13a"
                     /gene_synonym="Munc13-1; Unc13h1"
                     /inference="alignment:Splign:2.1.0"
     exon            3559..3655
                     /gene="Unc13a"
                     /gene_synonym="Munc13-1; Unc13h1"
                     /inference="alignment:Splign:2.1.0"
     exon            3656..3799
                     /gene="Unc13a"
                     /gene_synonym="Munc13-1; Unc13h1"
                     /inference="alignment:Splign:2.1.0"
     exon            3800..3883
                     /gene="Unc13a"
                     /gene_synonym="Munc13-1; Unc13h1"
                     /inference="alignment:Splign:2.1.0"
     exon            3884..3961
                     /gene="Unc13a"
                     /gene_synonym="Munc13-1; Unc13h1"
                     /inference="alignment:Splign:2.1.0"
     exon            3962..4050
                     /gene="Unc13a"
                     /gene_synonym="Munc13-1; Unc13h1"
                     /inference="alignment:Splign:2.1.0"
     exon            4051..4203
                     /gene="Unc13a"
                     /gene_synonym="Munc13-1; Unc13h1"
                     /inference="alignment:Splign:2.1.0"
     exon            4204..4327
                     /gene="Unc13a"
                     /gene_synonym="Munc13-1; Unc13h1"
                     /inference="alignment:Splign:2.1.0"
     exon            4328..4333
                     /gene="Unc13a"
                     /gene_synonym="Munc13-1; Unc13h1"
                     /inference="alignment:Splign:2.1.0"
     exon            4334..4372
                     /gene="Unc13a"
                     /gene_synonym="Munc13-1; Unc13h1"
                     /inference="alignment:Splign:2.1.0"
     exon            4373..4402
                     /gene="Unc13a"
                     /gene_synonym="Munc13-1; Unc13h1"
                     /inference="alignment:Splign:2.1.0"
     exon            4403..4459
                     /gene="Unc13a"
                     /gene_synonym="Munc13-1; Unc13h1"
                     /inference="alignment:Splign:2.1.0"
     exon            4460..4540
                     /gene="Unc13a"
                     /gene_synonym="Munc13-1; Unc13h1"
                     /inference="alignment:Splign:2.1.0"
     exon            4541..4688
                     /gene="Unc13a"
                     /gene_synonym="Munc13-1; Unc13h1"
                     /inference="alignment:Splign:2.1.0"
     exon            4689..4745
                     /gene="Unc13a"
                     /gene_synonym="Munc13-1; Unc13h1"
                     /inference="alignment:Splign:2.1.0"
     exon            4746..4838
                     /gene="Unc13a"
                     /gene_synonym="Munc13-1; Unc13h1"
                     /inference="alignment:Splign:2.1.0"
     exon            4839..4998
                     /gene="Unc13a"
                     /gene_synonym="Munc13-1; Unc13h1"
                     /inference="alignment:Splign:2.1.0"
     exon            4999..7985
                     /gene="Unc13a"
                     /gene_synonym="Munc13-1; Unc13h1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cccggtgctg aagcgacatg gccggcgacc gggccccagc gtaagctctg agccccgcgc
       61 tgcgcccgcc gcgctctggc cgcctggagt catgtctctg ctgtgcgtgg gagtcaaaaa
      121 agccaagttt gacggtgccc aagagaagtt caacacatac gtgacgctga aggtgcagaa
      181 cgtgaagagc actaccatag ctgtacgcgg cagccagccc agctgggagc aggacttcat
      241 gtttgagatc aaccgcctgg atctgggcct gacggtggag gtgtggaaca agggtctcat
      301 ctgggacaca atggtgggta ctgtgtggat cccacttcgg accatccgtc agtccaatga
      361 ggagggtccg ggagaatggc tgacactgga ctctcaggcc atcatggcag acagtgagat
      421 ctgtgggacc aaggacccca ccttccaccg catcctcctg gacgcacatt ttgagctgcc
      481 tttggacatc cccgaggagg aggcgcgcta ctgggccaag aagctggagc agctgaatgc
      541 catgcgtgac caagatgagt actcctttca ggaccagcag gacaagccac tgccggtgcc
      601 cagcagccag tgctgcaact ggaattactt tggctgggga gaacagaatg atgatcccga
      661 cagtgccgtg gatgaccggg acagtgatta taggagtgag acgagcaaca gcatcccacc
      721 gccttactac acgacttcgc agcccaatgc ttcggtgcac cagtactccg tgcggccacc
      781 ccctctgggg tcccgggagt cctacagcga ctccatgcac agctatgaag agttctctga
      841 gccgcgggca ctcagcccca caggcagcag ccgctatgct tccagtgggg agctgagcca
      901 gggcagctcc cagctgagtg aggacttcga cccggatgag cacagcctac agggctcaga
      961 gctggatgac gagagggacc gagattctta tcactcctgc cacagctctg tgagctacca
     1021 caaggactca ccccgctggg accaggatga ggaggacctg gaggacctgg aggacctgga
     1081 agatgaggag ttgcccgagg aagaggagtt ggaagaggag gagttggagg aggaggagga
     1141 gttggaggaa gaggaattgg agttggagga ggaggaggag gtgcctgatg acctggccag
     1201 ctatacccag caggaggaca ccactgtggc tgagcccaaa gagttcaagc ggatcagctt
     1261 cccaacagct gcacctcaga aggaagacaa agtttcagct gtgcccattg aggcccccga
     1321 tgtgtccaaa ggcatcccca aggcagccac acctgaagag aaggcagctg cggagtgtgc
     1381 acaggaagca gagcccccca agtctgagga gagtttcaga tctcgagagg ctgaggaggg
     1441 ccaggaaggg caggatgcca tgtccagggc caaagccaac tggttgcgag ccttcaacaa
     1501 ggtgcgcatg cagctgcagg aggcccgagg agaaggagag atgtccaagt ctctgtggtt
     1561 caaaggcggc cctggtggtg gccttatcat cattgacagc atgccagaca tcaggaagcg
     1621 gaagcccatt cccctcgtga gcgacctggc tatgtctctg gtccagtcac ggaaggcggg
     1681 catcacctcg gccttggcct ccagcacgtt gaacaatgaa gagttgaaaa accacgttta
     1741 caagaagacc ctgcaagcct taatctaccc catctcctgc accacgccgc acaacttcga
     1801 ggtgtggacg gccaccactc ccacctactg ctacgagtgc gaggggctgc tgtggggcat
     1861 cgcgcggcag ggcatgcgct gcaccgagtg cggcgttaag tgccacgaga agtgccaaga
     1921 cctgctcaac gcggactgcc tgcagcgggc ggctgagaag agttctaagc atggcgctga
     1981 agaccgcacg cagaacatca tcatggtgct gaaggaccgc atgaagatcc gcgagcgcaa
     2041 caagcctgag atcttcgagc tgatccagga ggtcttcgcg gtcaccaaga gcgcacacac
     2101 acagcagatg aaggccgtca agcagagtgt gctggatggc acatccaagt ggtctgccaa
     2161 aattagcatc acggtggtct gtgcccaggg cttgcaggcc aaggacaaga caggatccag
     2221 tgacccttat gtcaccgtcc aggttgggaa gaccaagaaa aggacaaaaa ccatctacgg
     2281 gaaccttaac ccagtgtggg aagagaattt ccactttgaa tgtcacaact cctctgaccg
     2341 gatcaaggtg cgtgtgtggg atgaagacga tgacatcaaa tcccgtgtga agcaaaggtt
     2401 taagagggag tctgatgact tcctagggca gacaatcatc gaggtgcgga cgcttagcgg
     2461 cgagatggat gtgtggtaca atctggacaa gagaacagac aagtctgctg tgtcgggcgc
     2521 cattcggctt cacatcagtg tggagatcaa aggggaggag aaggtggcac cctaccatgt
     2581 ccagtacacc tgtctgcatg agaacctgtt ccattttgtg acggatgtac agaacaatgg
     2641 cgtggtgaag attcccgatg ccaaaggtga cgacgcctgg aaggtttact atgatgagac
     2701 tgcccaggag atcgtggatg agtttgccat gcgctatggc gttgaatcca tctaccaagc
     2761 catgacccac tttgcctgcc tctcctccaa gtacatgtgc cctggggtac ccgctgtcat
     2821 gagcaccctg cttgccaaca tcaacgccta ctacgcacac accaccgcct ccaccaacgt
     2881 gtctgcctcc gaccgcttcg ctgcctccaa tttcgggaaa gagcgctttg tgaaacttct
     2941 ggaccagctg cacaactccc tgcggattga cctgtccatg taccggaaca acttcccggc
     3001 cagcagcccc gagcggctgc aggatctcaa gtccacagtg gacctgctta ccagcatcac
     3061 cttcttccgg atgaaggttc aggaactgca gagtccgcca cgtgccagtc aggtggtgaa
     3121 ggactgcgtg aaggcgtgcc tcaactccac ctacgagtac atcttcaaca actgtcatga
     3181 gctctatggc cgggagtacc agacagaccc ggccaagaag ggggaggtcc ccccggagga
     3241 gcaaggccct agcatcaaga acctggattt ctggtccaag ctcatcaccc tcatcgtgtc
     3301 tatcattgag gaggataaga actcctacac accctgcctc aatcagtttc cccaggagct
     3361 gaatgtgggg aagatcagtg ctgaggtgat gtggagcctg tttgcccagg acatgaagta
     3421 cgccatggag gaacacgaca agcaccggct gtgtaagagc gcagactaca tgaacctgca
     3481 tttcaaggtc aagtggttgt acaacgagta cgtggctgaa ctgcccacct tcaaggaccg
     3541 cgtgccggag taccctgcgt ggtttgagcc cttcgtcatc cagtggttag atgagaatga
     3601 ggaggtgtcc cgggacttcc tgcatggtgc actcgagcgg gacaagaagg atgggttcca
     3661 gcagacatcg gagcacgccc tgttctcttg ctcggtagtg gacgtcttct cccagctcaa
     3721 ccagagcttt gagatcatca agaagctgga gtgtcctgac ccccagattg tgggccacta
     3781 catgcggcgc tttgccaaga ccattagcaa tgtgcttctc cagtacgccg acatcgtctc
     3841 caaggacttc gcttcctact gctccaagga gaaggagaaa gtgccctgca tcctcatgaa
     3901 caacacacag cagctgcggg tgcagctgga gaagatgttc gaggcgatgg gtgggaagga
     3961 gctggacgcc gaggccagtg gaaccctgaa ggagctgcag gtgaaactca acaatgtcct
     4021 ggatgaactc agccacgtgt ttgccaccag cttccagcca cacatcgagg agtgtgtcag
     4081 acaaatgggt gacatcctaa gccaagtgaa gggcacgggc aacgtgcccg ccagtgcctg
     4141 cagcagcgtg gcacaggacg cggacaacgt gctacagccc atcatggatc ttctggacag
     4201 caacctcacc ctgtttgcca aaatctgtga gaagacggtt ctgaagcggg tgctgaagga
     4261 gctgtggaag ctggtgatga acaccatgga gaggaccatc gtcctgccgc cactcactga
     4321 ccagacgatg attggtaccc tcttgagaaa acatggcaag ggcctagaaa agggcagggt
     4381 gaaactgcca agccactcag acgggacaca aatgatcttc aatgccgcca aggagctggg
     4441 ccagctgtcc aaactgaagg atcacatggt gcgagaagaa gccaagagct tgaccccgaa
     4501 gcagtgtgcc gtggttgaac tggccctgga caccatcaag caatacttcc acgcgggggg
     4561 cgtgggcctc aagaagacct ttctcgagaa aagcccggac cttcagtccc tgcgctacgc
     4621 cctgtcgctc tacacacagg ccaccgacct gctcatcaaa accttcgtgc agacgcagtc
     4681 agcgcaggtc catggtggaa aggggactag gtttaccctt agtgaagacg tttgtcctga
     4741 gatgggctcg ggtgtggaag accctgtagg tgaagtatcc gtccacgtgg agctgttcac
     4801 gcatccggga actggggaac agaaggtcac agtgaaggtg gtggccgcca acgacctcaa
     4861 gtggcagact tctggcatct tccgtccgtt cattgaggtc aacatcgttg gacctcagct
     4921 cagcgacaag aaacgaaagt tcgccaccaa atcgaaaaac aacagctggg cgcccaaata
     4981 taacgagagc ttccagttct ccctgagcgc cgacgcggga cccgagtgct acgagttgca
     5041 ggtgtgcgtg aaggactact gcttcgcgcg cgaggaccgc acggtggggc tggcggtgct
     5101 gcagctgcgg gagctggctc agcgcgggag cgccgcgtgc tggctgccgc tcggccgccg
     5161 catccacatg gacgacacgg ggctcacagt gctgcgtatc ctgtcgcagc gcagcaatga
     5221 cgaggtggcc aaggagttcg tcaagctcaa gtccgacacg cgctcggccg aggagggcgg
     5281 tgccgcgcct gcgccctagc gcgcgttttg gcggcggggc ggggcctggg aagggatgag
     5341 gtcccgcctt ccgtaggcca ccgcctctcc agaggccacg cccctccacc tcagtggcct
     5401 tggctgggga ggccctgccc cctgcccctg gaaaagccat agagggtccg gtgcccaggg
     5461 catggactgg gcagggctgc ctgggaggct gggtcgcagc ctctggtgtc tgcaaaatac
     5521 agggcgggat ggactctcaa aagcacacgc gccctcggcg cacagctggg gctgcgggca
     5581 cccctctccg cggaccccgc cctcagcagc gggcccactg gcagctggcg accccctgct
     5641 cgtgtcacac agggcgggct gagctccgac gaatggccca gcccccatcg ctgctaaata
     5701 accattcaga atcttcctga ttcttccggg aggtcccgca ggccagggac cctcactgaa
     5761 ccctcaggct gccaaacaag attcctgtcc ttgctcttct ttctgagact ttagtcggaa
     5821 ccggcctagt caagaagggg ggcgttggag ttttcccgga gccaagagcc ccccattccg
     5881 ccttttctcc aaatccaggc aggaaataac agagagacca gaaactagga aagagggaga
     5941 gattgagaat atgaacgtgc atttctgagg gtcctgtgct tgtggtagtg ggacgtccct
     6001 tttctacttg gcttgaacca cagcatgttt cctgaatgtc acgtgcagtg acaggatagc
     6061 atgcaggctc tcatatatga tccaggtttc atccatggag ctcagaggtt acataccata
     6121 cattgatggg ggactcatag tggagctcag gagggagtga gtgactttca ctttaggaca
     6181 tctaacatgc ctaaagggaa agtggtctga acagagatca ctcatagaaa gtttctacat
     6241 tgctggcatt gtgtgacatg tgcaagtgcg tgcgcgtgca cgagtgcgag tgcgtgtgtt
     6301 ttccagccct agaatgttga agaaagtaca ttggaagtgt ttgtctagtg aattgtcctg
     6361 actcccaggc taggactgtt tgaaatttca ctttatttac ccagagacct cctattcatt
     6421 cttcaaccct cattacaaaa atcctccccg acccgtacaa aagaatttct caaggacttc
     6481 cccagcttat tcttgccctt ctctgatttg gccctgacgt catgagacta gggtgtctat
     6541 gcctggttct gtttctccta atccccagcc tcacttcaca ggagccccaa attccacagc
     6601 ataagcaggt cacagtaatt gaaggaagat tgtgccaact tgaattatgt gttgagaatg
     6661 gctgaggggt ctgcatgtga cccccaattc acacgcacgt ttttcattac agtttcttct
     6721 gacttgacgg acatccctat gtgggaggtg tccaagctga gccccaggac acaaaagaag
     6781 gaagcagagc agagggccga gctctgatct caggacagcc ccagatataa caacgtcctg
     6841 aaaggaagtt actccagggc ctggccttct gagctctcac cacctcccct ggcggcccat
     6901 cgctgctgga gtcctgcatt aaagcacact ggggtgcagc tccagtcccc acacgccagg
     6961 gatggttaga gactggtctg cagcaggctg gaatgagcca cagccccacc cgcaggagag
     7021 gcaggactct gagaggcaga tgccaggggg tgagggaggc tacagtgtgg gcagaagggg
     7081 gaggcaggaa gagcaggtgg aaggatctgg ggtccaggct catttatacc ctacctagaa
     7141 ttcctcagtt agggggctgg ggcgtgactc agcacctgcc actcatgcat tgttctaggt
     7201 tcatttccca acactgaaaa atcgaaaagg tcctgcatca gctcaccatg tctgggcgat
     7261 tcagcacttg aagggtcgcc ctctgcaccc cagtccgttt caggccccag ggcctgtcgc
     7321 ctcacctgcc ccatcagaca tgcaaaaaat caagtgcact cagaactcct ggggacctct
     7381 cccagcctgg actgcagacc tgtcaggtcc caagggcggc cctgctgggg gtggggtaag
     7441 gggtggggtt ggcctggctc cgcccacccc caccccagca tctttcagga ccaaagctgg
     7501 ctctgcagac ctgtctccct ctcctttttg ccttttatta ttttaaaaag tgaattcagt
     7561 tgagctgttt agtccgaccc ttctcagcca cagctgctgt gttgacgcaa gtgacaagcg
     7621 agggtcccct gcaccccaga aggttcttga cccctacccc tgccacgcag cccagggagg
     7681 ccctctgact cgcctttctt agcatgtgat tgcaaaggta gaatcccagc gtcgtgcttc
     7741 tggtttggtt ttttgggttc ttttttttct tttggaggat ttttttaagt atcaccccgt
     7801 tagtctagca tctttcaaga tgccctcaat tctcagtcaa cacgtgactt gggacacatg
     7861 ttcaaaatac ccggggagtg ttcatgagtg catggcgttg agatgttttg cactattctg
     7921 aatttttggt ctctgtaaaa aatattttat taacattaaa aaagaaagaa aaagaaaaaa
     7981 acaca
//
