LOCUS       NM_173323               1936 bp    mRNA    linear   ROD 13-FEB-2025
DEFINITION  Rattus norvegicus UDP glucuronosyltransferase family 2 member B7
            (Ugt2b7), mRNA.
ACCESSION   NM_173323
VERSION     NM_173323.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Abdullah,N.H. and Ismail,S.
  TITLE     Inhibition of UGT2B7 Enzyme Activity in Human and Rat Liver
            Microsomes by Herbal Constituents
  JOURNAL   Molecules 23 (10), 2696 (2018)
   PUBMED   30347696
  REMARK    GeneRIF: Meanwhile, only mitragynine and zerumbone inhibited
            ZDV(probe substrate to determine UGT2B7 activity) glucuronidation
            in RLM with IC50 values of 51.20 +/- 5.95 muM and 8.14 +/- 2.12
            microM, respectively, indicating a difference between the human and
            rat microsomal model so caution must be exercised when
            extrapolating inhibitory metabolic data from rats to humans.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Wang,F., Miao,M.X., Sun,B.B., Wang,Z.J., Tang,X.G., Chen,Y.,
            Zhao,K.J., Liu,X.D. and Liu,L.
  TITLE     Acute liver failure enhances oral plasma exposure of zidovudine in
            rats by downregulation of hepatic UGT2B7 and intestinal P-gp
  JOURNAL   Acta Pharmacol Sin 38 (11), 1554-1565 (2017)
   PUBMED   28770824
  REMARK    GeneRIF: Acute liver failure significantly increases the oral
            plasma exposure of zidovudine in rats, a result partly attributed
            to the impaired function and expression of hepatic UGT2B7 and
            intestinal P-gp.
REFERENCE   3  (bases 1 to 1936)
  AUTHORS   Gagez,A.L., Rouguieg-Malki,K., Sauvage,F.L., Marquet,P. and
            Picard,N.
  TITLE     Simultaneous evaluation of six human glucuronidation activities in
            liver microsomes using liquid chromatography-tandem mass
            spectrometry
  JOURNAL   Anal Biochem 427 (1), 52-59 (2012)
   PUBMED   22579593
REFERENCE   4  (bases 1 to 1936)
  AUTHORS   Kato,Y., Nakajima,M., Oda,S., Fukami,T. and Yokoi,T.
  TITLE     Human UDP-glucuronosyltransferase isoforms involved in haloperidol
            glucuronidation and quantitative estimation of their contribution
  JOURNAL   Drug Metab Dispos 40 (2), 240-248 (2012)
   PUBMED   22028316
  REMARK    GeneRIF: Data suggest that UGT2B7 is the predominant activity in
            liver microsomes for haloperidol O-glucuronidation (in both rat and
            human).
REFERENCE   5  (bases 1 to 1936)
  AUTHORS   Rowbotham,S.E., Illingworth,N.A., Daly,A.K., Veal,G.J. and
            Boddy,A.V.
  TITLE     Role of UDP-glucuronosyltransferase isoforms in 13-cis retinoic
            acid metabolism in humans
  JOURNAL   Drug Metab Dispos 38 (7), 1211-1217 (2010)
   PUBMED   20308471
REFERENCE   6  (bases 1 to 1936)
  AUTHORS   Brand,W., Boersma,M.G., Bik,H., Hoek-van den Hil,E.F., Vervoort,J.,
            Barron,D., Meinl,W., Glatt,H., Williamson,G., van Bladeren,P.J. and
            Rietjens,I.M.
  TITLE     Phase II metabolism of hesperetin by individual
            UDP-glucuronosyltransferases and sulfotransferases and rat and
            human tissue samples
  JOURNAL   Drug Metab Dispos 38 (4), 617-625 (2010)
   PUBMED   20056724
REFERENCE   7  (bases 1 to 1936)
  AUTHORS   Joseph,T.B., Wang,S.W., Liu,X., Kulkarni,K.H., Wang,J., Xu,H. and
            Hu,M.
  TITLE     Disposition of flavonoids via enteric recycling: enzyme stability
            affects characterization of prunetin glucuronidation across
            species, organs, and UGT isoforms
  JOURNAL   Mol Pharm 4 (6), 883-894 (2007)
   PUBMED   18052087
REFERENCE   8  (bases 1 to 1936)
  AUTHORS   Miley,M.J., Zielinska,A.K., Keenan,J.E., Bratton,S.M.,
            Radominska-Pandya,A. and Redinbo,M.R.
  TITLE     Crystal structure of the cofactor-binding domain of the human phase
            II drug-metabolism enzyme UDP-glucuronosyltransferase 2B7
  JOURNAL   J Mol Biol 369 (2), 498-511 (2007)
   PUBMED   17442341
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000014.1.
            
            On Dec 3, 2020 this sequence version replaced NM_173323.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U27518.1, SRR26643287.30115.1
                                           [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-742               JAXUCZ010000014.1  21251535-21252276
            743-891             JAXUCZ010000014.1  21253789-21253937
            892-1023            JAXUCZ010000014.1  21262058-21262189
            1024-1111           JAXUCZ010000014.1  21263791-21263878
            1112-1331           JAXUCZ010000014.1  21264535-21264754
            1332-1936           JAXUCZ010000014.1  21273847-21274451
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="14"
                     /map="14p21"
     gene            1..1936
                     /gene="Ugt2b7"
                     /gene_synonym="UDPGT 2B7; Ugt2b8"
                     /note="UDP glucuronosyltransferase family 2 member B7"
                     /db_xref="GeneID:286989"
                     /db_xref="RGD:708417"
     exon            1..742
                     /gene="Ugt2b7"
                     /gene_synonym="UDPGT 2B7; Ugt2b8"
                     /inference="alignment:Splign:2.1.0"
     CDS             19..1608
                     /gene="Ugt2b7"
                     /gene_synonym="UDPGT 2B7; Ugt2b8"
                     /EC_number="2.4.1.17"
                     /note="UDP-glucuronosyltransferase 2B8;
                     UDP-glucuronosyltransferase 2B7; UDPGT 2B8; UGT2B-RH4; UDP
                     glucuronosyltransferase 2 family, polypeptide B7"
                     /codon_start=1
                     /product="UDP-glucuronosyltransferase 2B7 precursor"
                     /protein_id="NP_775445.2"
                     /db_xref="GeneID:286989"
                     /db_xref="RGD:708417"
                     /translation="MPQKWISALLLLQISFCFRSGNCGKVLVWPLEYSHWMNLKIILD
                     ELVQRGHEVTVLRPSSSVFLDPKKASGLVYETFPTTSNNDEVEKFFTQWVNTWTYDVP
                     KYTCLRYYPSLNKMFGQFSDLWLQLCREVVSNKHLMAKLKESQFDVVLSDAVGPCGEL
                     IAEILQLPFVYSLRFGIGYGIEKYSAGQPFPPSYVPIILSGLSGQMTFMERVENMLCL
                     LYFDFWFESFPAKDWDPFFSEILGRPTTMVDTMKKAEIWLIRSYWDLEFPRPSLPNIE
                     FVGGLHCQPAKPLPKEMEDFAQSSGEHGVVVFSLGSMIRNITQERANTIASALAQIPQ
                     KVFWRFEGKKPDTLGPNTRVFKWIPQNDLLGHPKTKAFVTHGGANGIYESIHHGIPMV
                     GIPLFAEQRDNVAHMVAKGAAVSLDFHTMSSSDLLNALKAVINKPSYKKKVMWLSAIH
                     HDQPLKPLDRAVFWIEFVMRHKGAKHLRPLAHNLAWYQYHSLDVIGFLLACVLAIVLL
                     AVKCCLFLYRFFVKVAKNKRD"
     exon            743..891
                     /gene="Ugt2b7"
                     /gene_synonym="UDPGT 2B7; Ugt2b8"
                     /inference="alignment:Splign:2.1.0"
     exon            892..1023
                     /gene="Ugt2b7"
                     /gene_synonym="UDPGT 2B7; Ugt2b8"
                     /inference="alignment:Splign:2.1.0"
     exon            1024..1111
                     /gene="Ugt2b7"
                     /gene_synonym="UDPGT 2B7; Ugt2b8"
                     /inference="alignment:Splign:2.1.0"
     exon            1112..1331
                     /gene="Ugt2b7"
                     /gene_synonym="UDPGT 2B7; Ugt2b8"
                     /inference="alignment:Splign:2.1.0"
     exon            1332..1936
                     /gene="Ugt2b7"
                     /gene_synonym="UDPGT 2B7; Ugt2b8"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 aggactttga tcttcaggat gcctcagaag tggatttctg ctctgctcct actgcagata
       61 agtttctgct tcagatctgg gaactgtggg aaagtgttgg tgtggccatt ggaatacagt
      121 cactggatga atttaaagat aatactggat gaacttgtac agaggggaca tgaagtcact
      181 gttctcagac cttcatcttc tgtctttctt gatcccaaaa aagcatctgg tcttgtatat
      241 gaaacatttc ctacaacttc caataatgat gaagtagaaa agttttttac ccagtgggtg
      301 aatacgtgga catatgatgt gccaaaatat acatgtttga gatactaccc atctttgaac
      361 aaaatgtttg ggcaattttc tgatttgtgg ttacagcttt gtagagaagt tgtttcaaac
      421 aaacacctta tggcaaaact aaaggaatcc cagtttgatg tggtcctttc agatgctgtt
      481 ggtccctgtg gtgagctgat agctgagata ctgcagttac catttgtgta cagtcttcgc
      541 tttggtattg gctatggaat agaaaagtac agtgcgggac aacccttccc tccctcttat
      601 gtacctatta ttttgtcagg attaagtggt caaatgacat tcatggagag agtggaaaat
      661 atgctatgtt tactttattt tgatttttgg ttcgagtcat ttcctgctaa ggattgggat
      721 ccatttttca gcgaaatttt aggaagaccc actacaatgg ttgacacaat gaagaaagca
      781 gaaatatggc tcattcggtc ctactgggac ttggagtttc cccgcccatc cttgccaaac
      841 attgagtttg ttggaggact tcactgccaa cctgccaaac cattacctaa ggaaatggaa
      901 gactttgcac agagctcagg agaacatggt gtggtggtgt tttctctggg gtcgatgatc
      961 aggaacataa cacaagaaag ggctaacaca attgcatcag ccctggcaca aattccacaa
     1021 aaggtttttt ggagatttga gggcaagaaa ccagacacct taggacccaa tactcgggtg
     1081 ttcaagtgga taccccagaa tgaccttctt ggtcatccaa aaactaaggc ttttgtaact
     1141 catggtggag ccaatggcat ctatgagtca atccatcatg ggatccccat ggttggcatc
     1201 cctttatttg cagaacaacg tgataacgtt gctcacatgg tagccaaagg agcagctgtt
     1261 tctctagatt ttcatacaat gtccagttca gatttgctca acgcactgaa ggcagtcatc
     1321 aataaaccct cctataaaaa gaaagtgatg tggttgtcag ccattcacca tgaccagcct
     1381 ctgaagcctc tggacagagc agtcttctgg attgagtttg tcatgcgtca caaaggggcc
     1441 aagcatctga gaccacttgc acataacctt gcctggtatc agtaccactc tctggatgtg
     1501 attggattcc tgcttgcctg tgtgctggcc attgttttac ttgctgtaaa atgctgtctg
     1561 tttctttacc gattttttgt aaaggtggca aagaataaaa gggactaggg ttctttaaga
     1621 aggaaggctg tgatctgtgg tcgtgatggg catgactact ccctttattg ctggaactat
     1681 atatcagatg ctccttgttt tagaagacat tgagctaatt gatttatcta tagatgttac
     1741 actataagtt aagaaagtaa ccattttaaa tctttatttt ctcaaataaa ttgttttcat
     1801 attgattcat cgatgtgata gtgctgagga taatgataat ccctgaaaca ccaacttgct
     1861 tatgggcaaa gaataggaag cctaaacttt ttactgtgaa ttttcagata tttataaaat
     1921 aaaattgaat aaaatt
//
