LOCUS       NM_031795               3746 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Rattus norvegicus UDP-glucose ceramide glucosyltransferase (Ugcg),
            mRNA.
ACCESSION   NM_031795 XM_346741
VERSION     NM_031795.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3746)
  AUTHORS   Hasson,S.A., Kane,L.A., Yamano,K., Huang,C.H., Sliter,D.A.,
            Buehler,E., Wang,C., Heman-Ackah,S.M., Hessa,T., Guha,R.,
            Martin,S.E. and Youle,R.J.
  TITLE     High-content genome-wide RNAi screens identify regulators of parkin
            upstream of mitophagy
  JOURNAL   Nature 504 (7479), 291-295 (2013)
   PUBMED   24270810
REFERENCE   2  (bases 1 to 3746)
  AUTHORS   Amen,N., Mathow,D., Rabionet,M., Sandhoff,R., Langbein,L.,
            Gretz,N., Jackel,C., Grone,H.J. and Jennemann,R.
  TITLE     Differentiation of epidermal keratinocytes is dependent on
            glucosylceramide:ceramide processing
  JOURNAL   Hum Mol Genet 22 (20), 4164-4179 (2013)
   PUBMED   23748427
REFERENCE   3  (bases 1 to 3746)
  AUTHORS   Seymour,E.M., Bennink,M.R. and Bolling,S.F.
  TITLE     Diet-relevant phytochemical intake affects the cardiac AhR and nrf2
            transcriptome and reduces heart failure in hypertensive rats
  JOURNAL   J Nutr Biochem 24 (9), 1580-1586 (2013)
   PUBMED   23528973
REFERENCE   4  (bases 1 to 3746)
  AUTHORS   Nordstrom,V., Willershauser,M., Herzer,S., Rozman,J., von Bohlen
            Und Halbach,O., Meldner,S., Rothermel,U., Kaden,S., Roth,F.C.,
            Waldeck,C., Gretz,N., de Angelis,M.H., Draguhn,A., Klingenspor,M.,
            Grone,H.J. and Jennemann,R.
  TITLE     Neuronal expression of glucosylceramide synthase in central nervous
            system regulates body weight and energy homeostasis
  JOURNAL   PLoS Biol 11 (3), e1001506 (2013)
   PUBMED   23554574
REFERENCE   5  (bases 1 to 3746)
  AUTHORS   Jennemann,R., Kaden,S., Sandhoff,R., Nordstrom,V., Wang,S.,
            Volz,M., Robine,S., Amen,N., Rothermel,U., Wiegandt,H. and
            Grone,H.J.
  TITLE     Glycosphingolipids are essential for intestinal endocytic function
  JOURNAL   J Biol Chem 287 (39), 32598-32616 (2012)
   PUBMED   22851168
REFERENCE   6  (bases 1 to 3746)
  AUTHORS   Di Sano,F., Fazi,B., Citro,G., Lovat,P.E., Cesareni,G. and
            Piacentini,M.
  TITLE     Glucosylceramide synthase and its functional interaction with
            RTN-1C regulate chemotherapeutic-induced apoptosis in
            neuroepithelioma cells
  JOURNAL   Cancer Res 63 (14), 3860-3865 (2003)
   PUBMED   12873973
REFERENCE   7  (bases 1 to 3746)
  AUTHORS   Yamashita,T., Wada,R., Sasaki,T., Deng,C., Bierfreund,U.,
            Sandhoff,K. and Proia,R.L.
  TITLE     A vital role for glycosphingolipid synthesis during development and
            differentiation
  JOURNAL   Proc Natl Acad Sci U S A 96 (16), 9142-9147 (1999)
   PUBMED   10430909
REFERENCE   8  (bases 1 to 3746)
  AUTHORS   Wu,K., Marks,D.L., Watanabe,R., Paul,P., Rajan,N. and Pagano,R.E.
  TITLE     Histidine-193 of rat glucosylceramide synthase resides in a
            UDP-glucose- and inhibitor
            (D-threo-1-phenyl-2-decanoylamino-3-morpholinopropan-1-ol)-binding
            region: a biochemical and mutational study
  JOURNAL   Biochem J 341 (Pt 2) (Pt 2), 395-400 (1999)
   PUBMED   10393098
REFERENCE   9  (bases 1 to 3746)
  AUTHORS   Marks,D.L., Wu,K., Paul,P., Kamisaka,Y., Watanabe,R. and
            Pagano,R.E.
  TITLE     Oligomerization and topology of the Golgi membrane protein
            glucosylceramide synthase
  JOURNAL   J Biol Chem 274 (1), 451-456 (1999)
   PUBMED   9867864
REFERENCE   10 (bases 1 to 3746)
  AUTHORS   Ichikawa,S., Sakiyama,H., Suzuki,G., Hidari,K.I. and Hirabayashi,Y.
  TITLE     Expression cloning of a cDNA for human ceramide glucosyltransferase
            that catalyzes the first glycosylation step of glycosphingolipid
            synthesis
  JOURNAL   Proc Natl Acad Sci U S A 93 (10), 4638-4643 (1996)
   PUBMED   8643456
  REMARK    Erratum:[Proc Natl Acad Sci U S A. 1996 Oct 29;93(22):12654. doi:
            10.1073/pnas.93.22.12654.. PMID: 8901638]
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000005.1.
            
            On Oct 12, 2007 this sequence version replaced NM_031795.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26360194.513321.1,
                                           SRR26360172.1998116.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-112               JAXUCZ010000005.1  78828006-78828117
            113-254             JAXUCZ010000005.1  78844907-78845048
            255-357             JAXUCZ010000005.1  78848892-78848994
            358-459             JAXUCZ010000005.1  78850439-78850540
            460-572             JAXUCZ010000005.1  78851193-78851305
            573-751             JAXUCZ010000005.1  78854250-78854428
            752-838             JAXUCZ010000005.1  78855548-78855634
            839-1028            JAXUCZ010000005.1  78856417-78856606
            1029-3746           JAXUCZ010000005.1  78858008-78860725
FEATURES             Location/Qualifiers
     source          1..3746
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="5"
                     /map="5q24"
     gene            1..3746
                     /gene="Ugcg"
                     /note="UDP-glucose ceramide glucosyltransferase"
                     /db_xref="GeneID:83626"
                     /db_xref="RGD:621870"
     exon            1..112
                     /gene="Ugcg"
                     /inference="alignment:Splign:2.1.0"
     CDS             15..1199
                     /gene="Ugcg"
                     /EC_number="2.4.1.80"
                     /note="GCS; GLCT-1; glucosylceramide synthase;
                     UDP-glucose:N-acylsphingosine D-glucosyltransferase;
                     UDP-glucose:ceramide glycosyltransferase; glycosylceramide
                     synthase"
                     /codon_start=1
                     /product="ceramide glucosyltransferase"
                     /protein_id="NP_113983.1"
                     /db_xref="GeneID:83626"
                     /db_xref="RGD:621870"
                     /translation="MALLDLAQEGMALFGFVLFVVLWLMHFMSIIYTRLHLNKKATDK
                     QPYSKLPGVSLLKPLKGVDPNLINNLETFFELDYPKYEVLLCVQDHDDPAIEVCKKLL
                     GKYPNVDARLFIGGKKVGINPKINNLMPAYEVAKYDLIWICDSGIRVIPDTLTDMVNQ
                     MTERVGLVHGLPYVADRQGFAATLEQVYFGTSHPRSYISANVTGFKCVTGMSCLMRKD
                     VLDQAGGLIAFAQYIAEDYFMAKAIADRGWKFSMSTQVAMQNSGSYSISQFQSRMIRW
                     TKLRINMLPATIICEPISECFVASLIIGWAAHHVFRWDIMVFFMCHCLAWFIFDYIQL
                     RGVQGGTLCFSKLDYAVAWFIRESMTIYIFLSALWDPTISWRAGRYRLRCGGTAEEIL
                     DV"
     exon            113..254
                     /gene="Ugcg"
                     /inference="alignment:Splign:2.1.0"
     exon            255..357
                     /gene="Ugcg"
                     /inference="alignment:Splign:2.1.0"
     exon            358..459
                     /gene="Ugcg"
                     /inference="alignment:Splign:2.1.0"
     exon            460..572
                     /gene="Ugcg"
                     /inference="alignment:Splign:2.1.0"
     exon            573..751
                     /gene="Ugcg"
                     /inference="alignment:Splign:2.1.0"
     exon            752..838
                     /gene="Ugcg"
                     /inference="alignment:Splign:2.1.0"
     exon            839..1028
                     /gene="Ugcg"
                     /inference="alignment:Splign:2.1.0"
     exon            1029..3746
                     /gene="Ugcg"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cagcgggccg ggggatggcg ctgctggacc tggcccagga gggaatggcc ttgttcggct
       61 tcgtgctctt cgtggtgctg tggctgatgc atttcatgtc catcatctac acccggttac
      121 acctcaacaa gaaggcaaca gacaaacagc cgtacagcaa gctccctgga gtctctcttc
      181 tgaaaccact gaagggggtg gaccctaacc taatcaacaa cttggagaca ttctttgaac
      241 tggattatcc caaatatgaa gtactccttt gtgtacaaga tcatgatgat ccagccattg
      301 aggtgtgtaa gaaactgctt ggaaaatacc caaacgttga tgctagatta tttataggtg
      361 gtaaaaaggt tggcattaat cctaaaatta ataatttgat gccagcatat gaagttgcaa
      421 agtatgatct catatggatt tgtgatagcg gaatacgagt cattccagac acattaactg
      481 acatggtcaa tcagatgacg gagagagtgg ggctggtcca cgggcttcca tacgtagctg
      541 acagacaagg ctttgctgcc accttagagc aggtatattt tggaacttca cacccaagat
      601 cctatatctc tgccaatgtg accggcttca agtgtgtgac ggggatgtct tgcttgatga
      661 ggaaggatgt gctagatcag gcaggagggc tcatagcctt tgctcagtac attgcggaag
      721 attacttcat ggccaaagca atagcggacc gaggctggaa gttttcaatg tctacgcaag
      781 ttgccatgca aaactctggt tcatactcca tttctcagtt tcaatccaga atgatcaggt
      841 ggaccaaatt gaggattaac atgcttcctg ccacaataat ttgtgagcca atttcagaat
      901 gctttgttgc cagtttaatt atcgggtggg cagcccacca cgtgttccga tgggatatca
      961 tggttttctt catgtgtcac tgcctggcat ggtttatatt tgactacatt caactcaggg
     1021 gtgtccaggg tggcacacta tgcttttcaa aacttgatta tgctgtggct tggttcatcc
     1081 gcgaatccat gacaatatac attttcttgt cggcactgtg ggaccctact ataagctgga
     1141 gagccggtcg ctacaggttg cgctgtggag ggacggccga ggagatcctg gatgtgtaac
     1201 aaggcctctg tggccgactg gacaggatgg aatggaagtg ttatagatta tgtttataga
     1261 gactttccag gacctgcctt cagtggctca tccgtgtatg tgtggtatcc gctctttaat
     1321 ttattcgcat ggcccttgca tctgtgaaaa gcaaaaaaac aaaagcaaaa catgtctgta
     1381 gtcgtggcca aacagtgtat tttatggagg tagagaatca agacaattgg ttctaggaag
     1441 tgggttttgt tgttgtttat ttttttaaac aaatatatat atatatatct atcaatacac
     1501 acacacacac acacacacac acacacacac acacacacga ctgctctgca gcagacacta
     1561 tgacagtatc cttcagtagg gaaagtttct tacctgagtt actcactctg aggtccgcac
     1621 gggcagaaca gcagctctgt gagagggaag gggcaggtgc tggccgcccc tgtgtgaagg
     1681 gaatgggttg tacagggagg cgctgacgga gaaacttttt catgctttat gcctacctct
     1741 tgtagttgtt gcagagcaaa tagaagttag aagttgtgat atggtagcta ggccttgcaa
     1801 aaacaaatgg gaaactttaa taataaaaga gccgagtcta gtatttatat aaatctgtga
     1861 ggtttttttt gttttgtttt tttgtttttg ttttgttttg ttttattttt tggtctcatt
     1921 gcaatgaacc aaaagtggtt gagattggtt tttagtggta accatgaatt gaaggcatct
     1981 tttggaccaa ctgttgttgg ttctgtcttg aaccatatta atcactgtgc tgtaattagg
     2041 agagctgaat ctttccttcc ctgctcctcg cccagcccgc cccatctttc cttagctttt
     2101 tccatggggg ggggggggtg atcttatata agtgtaagtg gatgttttat agttgtaagg
     2161 aaaattcatt tcagacacag ttcacacagg agctattttc ttacccagta tgtctgtatt
     2221 gttaataaga atataatttc atgatacaag tatttaatat ctttttttaa gtgagtaaac
     2281 atattctagc tattggcaaa taaatcgcag tagagaatta ggagaggaca ggatgaggtt
     2341 tactcttcaa atcaatcagt atcaaactaa gtctactgtg tgtgtgtcgg ttggttgttt
     2401 tgttttgttt tttactataa tagtctatag tcttatttaa attggatttt tatgttcagt
     2461 ttgtatgaca tagactaggc agccgagttg taaatgcatg ctgtactttc ctctccccat
     2521 catgcagcta agcttgcttg tcagcctatg ctaacttgtg gtgaactgga cttttgtttt
     2581 aactaagcag agacgtaagg gacagtgcat cggccttttc ccctgtcttc tttaagcggt
     2641 gtttgctcat ccgtctgagt ttggtggtat gctgtccatc tgatgcttgc tgttcactcc
     2701 agcaccatga cagctcgcac cctgtgttcc tatggcttcc atctcagcac agcgtgtagc
     2761 ctattagcaa taacttccta cttatagaga acattttcat cttactgagc cagtccattt
     2821 acctaacgac tctacagaca gtctaaactg caatatacct gtaaatgtgt atatactata
     2881 aatacacact atccttacat ttttctgggc caaacggcag ttccttcccc ttctcttgct
     2941 cctccccttt ccattaatat tcattctctc tctctctccc tctctccctc tctccctctc
     3001 cctctctccc tccctctctc tctctctctc tctctctctc tctctctctc tctctctcct
     3061 tctctccctc tctctctctc tctctctttc tcgccttatg atgaatttgt ttgaagggca
     3121 ttttctccgt gagcaaaggc ttggatgcat attccagttt ttattttttt ttctgtgaaa
     3181 tgggtactat taccccctga ggaaaagttg cacagtgaga accagtctag ttatgaccca
     3241 ctacatgttt atttttttac attactctct cacgagctga gttttgctga gtgcgtttgt
     3301 cggtttgttt agtggcgttt ggtggaagcc cttctttgcg ctgctgaagt gaaatcttgc
     3361 tgactagtta gcttttttgt tgatctcagt atgggcaaag aaacaaaatc caaatggctg
     3421 gacagacttc tgtaaatatt tttaaaaaaa aaaaaacaaa tgttctgtgt gtgtgggttt
     3481 cgtgtggtcg tgaagtattt ccttaaagcg aagcctcgag ggtgcggaga ggaactcttt
     3541 gtttagcaag tttggagtga agataaaatg gtaagaaata ctgtaaatgt cgaggcgcca
     3601 caccagaact gtaggagtcc aaatttataa taaactgtct ttttaaaaag aaaacaatta
     3661 agtgtctaga atatatcatg ttttattatt taaaatcatt gtcttaagtt tttttgttga
     3721 gagaataaaa atttgaatac aatcaa
//
