LOCUS       NM_001271198            5627 bp    mRNA    linear   ROD 26-FEB-2024
DEFINITION  Rattus norvegicus ubiquitination factor E4B (Ube4b), mRNA.
ACCESSION   NM_001271198 XM_001075491 XM_233679
VERSION     NM_001271198.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 5627)
  AUTHORS   Gireud-Goss M, Reyes S, Tewari R, Patrizz A, Howe MD, Kofler J,
            Waxham MN, McCullough LD and Bean AJ.
  TITLE     The ubiquitin ligase UBE4B regulates amyloid precursor protein
            ubiquitination, endosomal trafficking, and amyloid beta42
            generation and secretion
  JOURNAL   Mol Cell Neurosci 108, 103542 (2020)
   PUBMED   32841720
  REMARK    GeneRIF: The ubiquitin ligase UBE4B regulates amyloid precursor
            protein ubiquitination, endosomal trafficking, and amyloid beta42
            generation and secretion.
REFERENCE   2  (bases 1 to 5627)
  AUTHORS   Starita LM, Pruneda JN, Lo RS, Fowler DM, Kim HJ, Hiatt JB,
            Shendure J, Brzovic PS, Fields S and Klevit RE.
  TITLE     Activity-enhancing mutations in an E3 ubiquitin ligase identified
            by high-throughput mutagenesis
  JOURNAL   Proc Natl Acad Sci U S A 110 (14), E1263-E1272 (2013)
   PUBMED   23509263
REFERENCE   3  (bases 1 to 5627)
  AUTHORS   Nordquist KA, Dimitrova YN, Brzovic PS, Ridenour WB, Munro KA, Soss
            SE, Caprioli RM, Klevit RE and Chazin WJ.
  TITLE     Structural and functional characterization of the monomeric U-box
            domain from E4B
  JOURNAL   Biochemistry 49 (2), 347-355 (2010)
   PUBMED   20017557
REFERENCE   4  (bases 1 to 5627)
  AUTHORS   Beirowski B, Babetto E, Coleman MP and Martin KR.
  TITLE     The WldS gene delays axonal but not somatic degeneration in a rat
            glaucoma model
  JOURNAL   Eur J Neurosci 28 (6), 1166-1179 (2008)
   PUBMED   18783366
  REMARK    GeneRIF: Data show that Wld(S) delays optic nerve (ON)axonal
            degeneration in experimental rat glaucoma for at least 2 weeks,
            especially in proximal ON where wild-type axons are most severely
            affected.
REFERENCE   5  (bases 1 to 5627)
  AUTHORS   Kaneko-Oshikawa C, Nakagawa T, Yamada M, Yoshikawa H, Matsumoto M,
            Yada M, Hatakeyama S, Nakayama K and Nakayama KI.
  TITLE     Mammalian E4 is required for cardiac development and maintenance of
            the nervous system
  JOURNAL   Mol Cell Biol 25 (24), 10953-10964 (2005)
   PUBMED   16314518
REFERENCE   6  (bases 1 to 5627)
  AUTHORS   Okumura F, Hatakeyama S, Matsumoto M, Kamura T and Nakayama KI.
  TITLE     Functional regulation of FEZ1 by the U-box-type ubiquitin ligase
            E4B contributes to neuritogenesis
  JOURNAL   J Biol Chem 279 (51), 53533-53543 (2004)
   PUBMED   15466860
  REMARK    GeneRIF: E4B serves as a ubiquitin ligase for FEZ1 and thereby
            regulates its function but not its degradation
REFERENCE   7  (bases 1 to 5627)
  AUTHORS   Kaneko C, Hatakeyama S, Matsumoto M, Yada M, Nakayama K and
            Nakayama KI.
  TITLE     Characterization of the mouse gene for the U-box-type ubiquitin
            ligase UFD2a
  JOURNAL   Biochem Biophys Res Commun 300 (2), 297-304 (2003)
   PUBMED   12504083
REFERENCE   8  (bases 1 to 5627)
  AUTHORS   Mahoney JA, Odin JA, White SM, Shaffer D, Koff A, Casciola-Rosen L
            and Rosen A.
  TITLE     The human homologue of the yeast polyubiquitination factor Ufd2p is
            cleaved by caspase 6 and granzyme B during apoptosis
  JOURNAL   Biochem J 361 (Pt 3), 587-595 (2002)
   PUBMED   11802788
REFERENCE   9  (bases 1 to 5627)
  AUTHORS   Hatakeyama S, Yada M, Matsumoto M, Ishida N and Nakayama KI.
  TITLE     U box proteins as a new family of ubiquitin-protein ligases
  JOURNAL   J Biol Chem 276 (35), 33111-33120 (2001)
   PUBMED   11435423
REFERENCE   10 (bases 1 to 5627)
  AUTHORS   Meyer HH, Shorter JG, Seemann J, Pappin D and Warren G.
  TITLE     A complex of mammalian ufd1 and npl4 links the AAA-ATPase, p97, to
            ubiquitin and nuclear transport pathways
  JOURNAL   EMBO J 19 (10), 2181-2192 (2000)
   PUBMED   10811609
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JACYVU010000162.1.
            
            [WARNING] On Apr 3, 2024 this sequence was replaced by
            NM_001271198.2.
            
            On or before Sep 8, 2012 this sequence version replaced
            XM_001075491.3, XM_233679.5.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMD00132261, SAMD00132262
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-864               JACYVU010000162.1  74418945-74419808   c
            865-1051            JACYVU010000162.1  74390521-74390707   c
            1052-1187           JACYVU010000162.1  74377623-74377758   c
            1188-1275           JACYVU010000162.1  74375814-74375901   c
            1276-1420           JACYVU010000162.1  74374425-74374569   c
            1421-1649           JACYVU010000162.1  74372019-74372247   c
            1650-1791           JACYVU010000162.1  74363115-74363256   c
            1792-1892           JACYVU010000162.1  74361582-74361682   c
            1893-2007           JACYVU010000162.1  74359056-74359170   c
            2008-2091           JACYVU010000162.1  74356285-74356368   c
            2092-2148           JACYVU010000162.1  74351360-74351416   c
            2149-2265           JACYVU010000162.1  74350561-74350677   c
            2266-2364           JACYVU010000162.1  74350384-74350482   c
            2365-2478           JACYVU010000162.1  74349435-74349548   c
            2479-2677           JACYVU010000162.1  74347899-74348097   c
            2678-2816           JACYVU010000162.1  74345520-74345658   c
            2817-2916           JACYVU010000162.1  74340257-74340356   c
            2917-3044           JACYVU010000162.1  74338873-74339000   c
            3045-3143           JACYVU010000162.1  74337840-74337938   c
            3144-3379           JACYVU010000162.1  74334878-74335113   c
            3380-3506           JACYVU010000162.1  74331259-74331385   c
            3507-3651           JACYVU010000162.1  74329666-74329810   c
            3652-3786           JACYVU010000162.1  74324103-74324237   c
            3787-3978           JACYVU010000162.1  74321870-74322061   c
            3979-4153           JACYVU010000162.1  74319259-74319433   c
            4154-4300           JACYVU010000162.1  74317926-74318072   c
            4301-5627           JACYVU010000162.1  74316128-74317454   c
FEATURES             Location/Qualifiers
     source          1..5627
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="5"
                     /map="5q36"
     gene            1..5627
                     /gene="Ube4b"
                     /gene_synonym="ufd2a"
                     /note="ubiquitination factor E4B"
                     /db_xref="GeneID:298652"
                     /db_xref="RGD:1304738"
     exon            1..864
                     /gene="Ube4b"
                     /gene_synonym="ufd2a"
                     /inference="alignment:Splign:2.1.0"
     CDS             841..4362
                     /gene="Ube4b"
                     /gene_synonym="ufd2a"
                     /EC_number="2.3.2.27"
                     /note="ubiquitination factor E4B, UFD2 homolog;
                     ubiquitination factor E4B (UFD2 homolog, yeast)"
                     /codon_start=1
                     /product="ubiquitin conjugation factor E4 B"
                     /protein_id="NP_001258127.1"
                     /db_xref="GeneID:298652"
                     /db_xref="RGD:1304738"
                     /translation="MEELSADEIRRRRLARLAGGQTSQPTTPLTSPQRENPPGPPIAA
                     SVPGPSQSLGLNVHNTTPATSPIGAAGVAHRSQSSEGVSSLSSSPSNSLETQSQSLSR
                     SQSMDIDGVSCEKSMSQVDVDSGIENMEVDENDRREKRSLSDKEPSSGPEVSEEQALQ
                     LVCKIFRVSWKDRDRDVIFLSSLSAQFKQNPKEVFSDFKDLIGQILMEVLMMSTQTRD
                     ENPFASLTATSQPIATAARSPDRNLMLNTGSSSGTSPMFCSLGSFSASSLSSLGASGG
                     ASTWDSYSDHFTIETCKETDMLNYLIECFDRVGIEEKKAPKMCSQPAVSQLLSNIRSQ
                     CISHTALVLQGSLTQPRSLQQPSFLVPYMLCRNLPYGFIQELVRTTHQDEEVFKQIFI
                     PILQGLARAAKECSLDSDYFKYPLMALGELCETKFGKTHPMCNLVASLPLWLPKSLSP
                     GSGRELQRLSYLGAFFSFSVFAEDDAKVVEKYFSGPAITLENTRVVSQSLQHYLELGR
                     QELFKILHSILLNGETREAALSYMAAIVNANMKKAQMQADDRLVSTDGFMLNLLWVLQ
                     QLSTKIKLETVDPTYIFHPRCRITLPNDETRINATMEDVNEWLTELYGDQPPFSEPKF
                     PTECFFLTLHAHHLSILPSCRRYIRRLRAIRELNRTVEDLKNNESQWKDSPLATRHRE
                     MLKRCKTQLKKLVRCKACADAGLLDESFLRRCLNFYGLLIQLMLRVLDPAYPDITLPL
                     NSEVPKVFAALPEFYVEDVAEFLFFIVQYSPQVLYEPCTQDIVMFLVVMLCNQNYIRN
                     PYLVAKLVEVMFMTNPSVQPRTQKFFEMIENHPLSTKLLVPSLMKFYTDVEHTGATSE
                     FYDKFTIRYHISTIFKSLWQNIAHHGTFMEEFNSGKQFVRYINMLINDTTFLLDESLE
                     SLKRIHEVQEEMKNQEQWDQLPRDQQQARQSQLAQDERVSRSYLALATETVDMFHLLT
                     KQVQKPFLRPELGPRLAAMLNFNLQQLCGPKCRDLKVENPEKYGFEPKKLLDQLTDIY
                     LQLDCARFAKAIADDQRSYSKELFEEVISKMRKAGIKSTIAIEKFKLLAEKVEEIVAK
                     NARAEIDYSDAPDEFRDPLMDTLMTDPVRLPSGTIMDRSIILRHLLNSPTDPFNRQML
                     TESMLEPVPELKEQIQAWMREKQSSDH"
     exon            865..1051
                     /gene="Ube4b"
                     /gene_synonym="ufd2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1052..1187
                     /gene="Ube4b"
                     /gene_synonym="ufd2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1188..1275
                     /gene="Ube4b"
                     /gene_synonym="ufd2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1276..1420
                     /gene="Ube4b"
                     /gene_synonym="ufd2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1421..1649
                     /gene="Ube4b"
                     /gene_synonym="ufd2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1650..1791
                     /gene="Ube4b"
                     /gene_synonym="ufd2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1792..1892
                     /gene="Ube4b"
                     /gene_synonym="ufd2a"
                     /inference="alignment:Splign:2.1.0"
     exon            1893..2007
                     /gene="Ube4b"
                     /gene_synonym="ufd2a"
                     /inference="alignment:Splign:2.1.0"
     exon            2008..2091
                     /gene="Ube4b"
                     /gene_synonym="ufd2a"
                     /inference="alignment:Splign:2.1.0"
     exon            2092..2148
                     /gene="Ube4b"
                     /gene_synonym="ufd2a"
                     /inference="alignment:Splign:2.1.0"
     exon            2149..2265
                     /gene="Ube4b"
                     /gene_synonym="ufd2a"
                     /inference="alignment:Splign:2.1.0"
     exon            2266..2364
                     /gene="Ube4b"
                     /gene_synonym="ufd2a"
                     /inference="alignment:Splign:2.1.0"
     exon            2365..2478
                     /gene="Ube4b"
                     /gene_synonym="ufd2a"
                     /inference="alignment:Splign:2.1.0"
     exon            2479..2677
                     /gene="Ube4b"
                     /gene_synonym="ufd2a"
                     /inference="alignment:Splign:2.1.0"
     exon            2678..2816
                     /gene="Ube4b"
                     /gene_synonym="ufd2a"
                     /inference="alignment:Splign:2.1.0"
     exon            2817..2916
                     /gene="Ube4b"
                     /gene_synonym="ufd2a"
                     /inference="alignment:Splign:2.1.0"
     exon            2917..3044
                     /gene="Ube4b"
                     /gene_synonym="ufd2a"
                     /inference="alignment:Splign:2.1.0"
     exon            3045..3143
                     /gene="Ube4b"
                     /gene_synonym="ufd2a"
                     /inference="alignment:Splign:2.1.0"
     exon            3144..3379
                     /gene="Ube4b"
                     /gene_synonym="ufd2a"
                     /inference="alignment:Splign:2.1.0"
     exon            3380..3506
                     /gene="Ube4b"
                     /gene_synonym="ufd2a"
                     /inference="alignment:Splign:2.1.0"
     exon            3507..3651
                     /gene="Ube4b"
                     /gene_synonym="ufd2a"
                     /inference="alignment:Splign:2.1.0"
     exon            3652..3786
                     /gene="Ube4b"
                     /gene_synonym="ufd2a"
                     /inference="alignment:Splign:2.1.0"
     exon            3787..3978
                     /gene="Ube4b"
                     /gene_synonym="ufd2a"
                     /inference="alignment:Splign:2.1.0"
     exon            3979..4153
                     /gene="Ube4b"
                     /gene_synonym="ufd2a"
                     /inference="alignment:Splign:2.1.0"
     exon            4154..4300
                     /gene="Ube4b"
                     /gene_synonym="ufd2a"
                     /inference="alignment:Splign:2.1.0"
     exon            4301..5627
                     /gene="Ube4b"
                     /gene_synonym="ufd2a"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gggagagggg gtggggcagg gggcggggac aggatgcaag cgaagaggaa atcagcggcg
       61 cgacgccttc tgcttattgg ctggtgagtc cgggagccag aagccccgcc tccccagcga
      121 gggtcacgtg atccctttca aagatggccg ccctgttgtt ttgatgaata atacttggtg
      181 gggcgagggg gtaagagtag ggttggaggg gtaggaggtt ttacgcttac agcgagcgct
      241 tcgccagtcc catccctccc ctccccctac caaggttctt gcgtggaggc ggggcgagcc
      301 tggcctgctt ttagagggga ggggcttccc ggtacggtgc tgggctctgc cttggctgct
      361 gtagggtcgc agcgccttgg gggcatccac tgtgcagtat agccgttccc gccaggggcc
      421 aggggtggga ccctgagcag ggtctgcggg agaaaaacgc agctgccgcg tcaccgccac
      481 gaagacacgg cgcgcagcgg gcacgagccg agctggagac cgcgccgccc gctctgggta
      541 acctgggaag ctgaggggag tcagtggcgc ctggagagga ccctgtagca gtggccgtgg
      601 ccagaggcgg cagctctggg cgcttgcagc tggagaagcc tagggcagtc cgagtgaccg
      661 aagccaaggc gattcagtgc ctcgtgttct tagtttttaa cctctgacta tgcaattctg
      721 aaacctcccc cattggggga ccagacagcc ggatagatac cttccactct cctttgctcc
      781 cgccctggtc tcaagaactg aaggatctct ctaacgcctt tcaccattaa gaggaaagcg
      841 atggaggagc tgagcgctga cgagattcga cggaggcgcc tggcacgact tgctggtgga
      901 cagacctccc agccgaccac cccgcttaca tctccccaga gggagaaccc tccaggacct
      961 ccaatagctg catcagtccc aggcccctcc cagagccttg gtctcaatgt ccacaacacg
     1021 accccagcta cctcccccat aggtgcagca ggagttgccc atcggagcca gagcagtgaa
     1081 ggcgtcagtt ctcttagcag ctcaccttcc aacagccttg agacgcaatc tcagtccctc
     1141 tcacgttccc agagcatgga tatcgatggt gtctcctgtg agaagagcat gtcccaggtg
     1201 gatgtggatt ctggaatcga gaatatggag gtggacgaga acgaccgaag agaaaaacgg
     1261 agcctgagtg acaaggagcc ttcttcaggt cctgaagtgt ctgaagagca ggccttacag
     1321 ctggtctgta agatcttccg tgtctcctgg aaggaccgcg acagagatgt catcttcctt
     1381 tcttctctct ctgcgcagtt taaacagaac ccaaaagaag tgttctctga ttttaaggat
     1441 ctgattggcc agattttaat ggaagtgctg atgatgtcca ctcagacacg agatgaaaat
     1501 ccgtttgcca gtctgacggc cacatcccag cccatcgcca cggcagctcg gtcaccagac
     1561 agaaacctca tgctgaacac aggctccagt tcggggacaa gtcccatgtt ctgcagcttg
     1621 ggttccttca gcgccagctc actctccagt ttgggagcct ctggtggagc aagtacctgg
     1681 gattcttaca gtgaccattt caccattgaa acctgcaaag agacggacat gctgaactac
     1741 ctcatcgagt gctttgaccg agttggaata gaggaaaaaa aagcaccaaa gatgtgcagc
     1801 cagccagcag tcagccagct tctgagcaac atccgctcgc agtgcatctc ccacaccgcg
     1861 ctagtgctac aagggtccct cacacagccc aggtccttgc aacagccatc cttcctcgtg
     1921 ccgtacatgc tctgcaggaa tctcccgtac ggcttcattc aagagctggt gaggaccact
     1981 caccaggatg aagaggtgtt caagcagatc tttatcccca ttttacaagg cctggcgcgt
     2041 gctgccaaag agtgctcctt ggatagcgac tactttaagt accccctcat ggcactgggt
     2101 gaactctgtg aaaccaagtt cgggaagaca caccctatgt gcaatttggt cgcctcctta
     2161 cccctgtggt tgccgaagtc cttaagccct ggttctgggc gggagctgca gagactctcc
     2221 tacttagggg ccttctttag cttctctgtc ttcgcagagg acgatgcaaa ggtggttgag
     2281 aaatacttct cagggcctgc cattaccctg gagaacaccc gtgtggtcag ccagtcacta
     2341 cagcattacc tggagctggg acggcaagag cttttcaaga ttctgcatag tattttgtta
     2401 aacggcgaaa ctcgtgaagc agcactcagt tacatggcag ccattgtcaa tgccaatatg
     2461 aagaaagcgc agatgcaggc agatgatcgg ttggtgtcta cagacgggtt catgctgaat
     2521 ctcctttggg tcctgcagca gctaagtaca aaaatcaagt tagaaacggt cgaccccact
     2581 tacatattcc acccgagatg tcggatcact ctgccaaacg atgagacacg gataaatgca
     2641 acgatggagg atgtgaacga gtggctgaca gagctctatg gagatcagcc tccattttct
     2701 gagccaaaat tccctacaga atgcttcttt ctcaccctgc acgctcacca tctctctatc
     2761 ctgcctagtt gtcgtcgcta cattcgccgg ctccgagcca tccgtgagct caatagaacc
     2821 gtggaagatt tgaaaaataa tgaaagccag tggaaagatt ccccactggc caccagacac
     2881 cgcgaaatgc tgaagcgctg taaaactcag cttaagaaac tggtacggtg caaggcctgt
     2941 gctgacgctg gcttacttga cgagagcttc ctaagaagat gtctgaattt ttatggcctt
     3001 ctcattcagc tgatgctgcg cgtcctggac cctgcatatc ctgacataac actgccttta
     3061 aattcagaag tccccaaggt atttgcagca ttgcctgagt tttatgtaga agatgttgct
     3121 gaatttttat tttttattgt acaatactct cctcaggtgc tttatgagcc ctgcactcag
     3181 gacattgtga tgttcctcgt tgtgatgctg tgcaaccaga actacatccg aaacccttac
     3241 ctagtggcca agctcgtgga ggtcatgttt atgaccaacc cctctgttca gccgagaact
     3301 cagaagtttt ttgagatgat tgagaaccat cctctctcta ccaaattgct ggtaccgtcc
     3361 ctcatgaagt tctatacaga cgtcgagcat actggagcca ccagcgagtt ctatgacaag
     3421 ttcacaattc gctatcacat cagcactatt tttaaaagcc tttggcaaaa catagctcac
     3481 catggcacct tcatggagga gttcaattct ggaaagcagt tcgttcggta tatcaacatg
     3541 ctgataaatg acacgacctt tttactggac gaaagtctgg agtctctgaa gcggatccat
     3601 gaagtgcaag aggagatgaa gaaccaagag cagtgggacc agctgccccg ggatcagcag
     3661 caggcccggc agtctcagct tgctcaggac gagcgtgttt cccgttccta tctcgccctg
     3721 gcgactgaaa ccgtggacat gttccatctc ctcaccaagc aagtccagaa gccgttcctg
     3781 aggccagaac ttggtccccg gttagcagcc atgctgaact ttaacctgca gcagctctgc
     3841 gggcccaagt gccgggacct caaagtggaa aacccagaga agtacggttt tgagccaaag
     3901 aagctgttgg accaactgac ggatatctac ttacagctgg actgtgctcg ctttgctaaa
     3961 gccatcgctg atgaccagcg atcctacagc aaggaactgt tcgaagaagt catttcaaag
     4021 atgcggaagg caggaatcaa atccaccatc gcaatagaaa agtttaagct tcttgcagag
     4081 aaagtggagg aaatcgtggc aaagaatgct cgggcggaaa tagactacag tgacgccccg
     4141 gacgagttca gagaccctct gatggacacc ctgatgactg atcccgtgag actgccctct
     4201 gggaccatca tggaccgttc tatcatcctg cggcatctgc tcaactcccc caccgacccc
     4261 ttcaaccgcc agatgctgac tgagagcatg ctggagccag tgccagagct aaaggagcag
     4321 attcaggcct ggatgagaga gaagcagagc agtgaccact aagtgtccca tcccatcgca
     4381 tcctcagctg gaagccgcca gccagccagc caggccaaag cagcatccgc ctcgaagctg
     4441 cttccaacca gatgtgagca gatcctgaga acccaccccg agtgaccaag cagtggagat
     4501 ggagggacat gacagaagag gattcctgta catatattta agtgacaaca gtcaagcttg
     4561 agggacgaat tttccatggc ttgtgtagta aagcgcgccc ttcgcctgtc gcccctgggg
     4621 ctgcggcagc ggaagtgttt agtgacgagt gggtctgggc agcttcccct gagcttacgt
     4681 ttttgttttg tttttttcga tgtgactaga gaactcggac attttgctgc taaagaatct
     4741 cccctccctc acccactggc actgctgtgg gatttttaaa gagacacaga cccagactcc
     4801 tttcccggcc ctccaaacat gtattctgtg aggtaactgt gcctgcgacg aagtgttaac
     4861 ctggggatcg tatttttata tcatattcac agacttgttt ttttaattgg tgttagatga
     4921 catgattaat aaaaaaggca agatattttc agaatttgaa tttcaggttt tttttttctt
     4981 ttgaaatagc cctttaaatt tttttattat aaacaaaatg aagagaaccc tgtgggcctg
     5041 gtccttctac cacccctcag ttatgatcct gagaagcagg tgcgacagca ggctggctgg
     5101 agtgaatcag gactcgtgtg acttgtgggg tttgggggag gtgagggttg tgttttgttt
     5161 tctgttggtt tttttttttt tttttttttt ttaatcctat gtgacaattc caatcctaag
     5221 ccgttatcct ctgaggatga ataagttcct caaccctggg ttcttttgta tgctgtgtcc
     5281 ttttggggtt tggccttacc aaagcaaaaa aaaaaaaaaa aaaaaaaaaa aaagggtgca
     5341 agaaatgtgg aaatctgtct gcttgtttac atgcccggac aaacaccacc acgtaaccac
     5401 gtaggcgcac tcgagcagaa gtactaccgc tccctacgca acatacttga attcttaaat
     5461 ttcctttggg gtgaaaaagg atttgaaacc ataaagtgtt ttaaagaaat gaaatcttca
     5521 gaaacatact ttttctcctc ttttccttcc tcctcctctt cacagtctgt gctctgttca
     5581 gttcccaaca caaacgacaa taaacggtga tgcccattca gaaggaa
//
