LOCUS       XM_221132               5373 bp    mRNA    linear   ROD 11-JUN-2023
DEFINITION  PREDICTED: Rattus norvegicus ubiquitin-conjugating enzyme E2O
            (Ube2o), transcript variant X1, mRNA.
ACCESSION   XM_221132
VERSION     XM_221132.10
DBLINK      BioProject: PRJNA677964
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
COMMENT     MODEL REFSEQ:  This record is predicted by automated computational
            analysis. This record is derived from a genomic sequence
            (NC_051345.1) annotated using gene prediction method: Gnomon,
            supported by EST evidence.
            Also see:
                Documentation of NCBI's Annotation Process
            
            [WARNING] On Jan 18, 2024 this sequence was replaced by
            NM_001427322.1.
            
            On Jan 21, 2021 this sequence version replaced XM_221132.9.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider         :: NCBI RefSeq
            Annotation Status           :: Updated annotation
            Annotation Name             :: GCF_015227675.2-RS_2023_06
            Annotation Pipeline         :: NCBI eukaryotic genome annotation
                                           pipeline
            Annotation Software Version :: 10.1
            Annotation Method           :: Best-placed RefSeq; Gnomon;
                                           cmsearch; tRNAscan-SE
            Features Annotated          :: Gene; mRNA; CDS; ncRNA
            Annotation Date             :: 06/06/2023
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5373
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN/NHsdMcwi"
                     /db_xref="taxon:10116"
                     /chromosome="10"
                     /sex="male"
                     /tissue_type="kidney"
                     /geo_loc_name="USA: Wisconsin, Milwaukee, Medical College
                     of Wisconsin"
                     /collection_date="2019-03-08"
                     /collected_by="Rebecca Schilling"
     gene            1..5373
                     /gene="Ube2o"
                     /note="ubiquitin-conjugating enzyme E2O; Derived by
                     automated computational analysis using gene prediction
                     method: Gnomon. Supporting evidence includes similarity
                     to: 48 ESTs, 1 long SRA read, 4 Proteins, and 100%
                     coverage of the annotated genomic feature by RNAseq
                     alignments, including 238 samples with support for all
                     annotated introns"
                     /db_xref="GeneID:303689"
                     /db_xref="RGD:1310297"
     CDS             65..3940
                     /gene="Ube2o"
                     /codon_start=1
                     /product="(E3-independent) E2 ubiquitin-conjugating enzyme
                     isoform X1"
                     /protein_id="XP_221132.5"
                     /db_xref="GeneID:303689"
                     /db_xref="RGD:1310297"
                     /translation="MADPAAPAPAPAPAQAQAAAAPTPAAAPAAAPPPAPATDSASGP
                     SSDSGPEAGSQRLLFSHDLVSGRYRGSVHFGLVRLIHGEDSDSEGEEDGRGSSGCSEA
                     GGAGHEEGRASPLRRGYVRVQWYPEGVKQHVKETKLKLEDRSVVPRDVVRHMRSTDSQ
                     CGTVIDVNIDCAVKLIGTNCIIYPVNSKDLQHIWPFMYGDYIAYDCWLGKVYDLKNQI
                     ILKLSNGARCSMNTEDGAKLYDVCPHVSDSGLFFDDSYGFYPGQVLIGPAKIFSSVQW
                     LSGVKPVLSTKSKFRVVVEEVQVVELKVTWITKSFCPGGTDSVSPPPSVIAQENLGRV
                     KRLGCFDHAQRQLGERCLYVFPAKVEPAKIAWECPEKNCAQGEGSMAKKVKRLLKKQV
                     VRIMSCTPDAQCSRDHAMEDPDKKGEARAGSEVGSASPEEQLDGSASPVEMQDEGSEE
                     LHETGEPLPPFLLKEGGDEGLHSAEQDADDEAADDTDDTSSVTSSASSTTSSQSGSGT
                     GRKKSIPLSIKNLKRKHKRKKNKITRDFKPGDRVAVEVVTTMTSADVMWQDGSVECNI
                     RSNDLFPVHHLDNNEFCPGDFVVDKRVQSCPDPAVYGVVQSGDHVGRTCMVKWFKLRP
                     SGDDVELIGEEEDVSVYDIADHPDFRFRTTDIVIRIGNTEDGALPKEDEPSVGQVARV
                     DVSSKVEVVWADNSKTIILPQHLYNIESEIEESDYDSVEGSTSGASSDEWEDDSDSWE
                     TDNGLVDDEHPKIEDLAAILPTEQPAAPEEDKGVVISEEAATAAIQGAVAMAAPVAGL
                     MEKAGKDGPPKSFRELKEAIKILESLKNMTVEQLLTGSPTSPTVEPEKPTREKKFLDD
                     IKKLQENLKKTLDNVAIAEEEKMEAVPDTERKEDKPEVQSPVKAEWPSETPVLCQQCG
                     GRPGVTFTSAKGEVFSVLEFAPSNHSFKKIEFQPPEAKKFFSTVRKEMALLATSLPDG
                     IMVKTFEDRMDLFSALIKGPTRTPYEDGLYLFDIQLPNIYPAVPPHFCYLSQCSGRLN
                     PNLYDNGKVCVSLLGTWIGKGTERWTSKSSLLQVLISIQGLILVNEPYYNEAGFDSDR
                     GLQEGYENSRCYNEMALIRVVQSMTQLVRRPPEVFEQEIRQHFSVGGWRLVNRIESWL
                     ETHVVLERAQALPNGVPKDSSSLEPQAAAELSDSGREEPEDVGMTPGEASQGSDSEGG
                     AQGPASASREHPEQTETAPDASAPPSVRPKRRRKSYRSFLPEKSGYPDIGFPLFPLSK
                     GFIKSIRGVLTQFRAALLEAGMPESTEDK"
ORIGIN      
        1 tgccgccgcc gctaccgccg ccgctgtcgc tgccgccgcc gcccgagact cgcgcagagc
       61 agttatggcg gatcccgcag cgcccgcacc cgcacccgct cccgcccagg cccaggccgc
      121 cgccgccccg accccggccg cagcccccgc cgccgccccg ccgcccgcgc ccgccacgga
      181 ctcggcctca gggccgtcct ccgactccgg ccccgaagcg ggctcgcagc gcctgctgtt
      241 ctctcacgac ctggtgtccg gccgttaccg cggctcggtg cacttcgggc tggtgcgcct
      301 catccacggt gaggactcgg actcggaggg cgaggaggac ggccgcggca gctcgggctg
      361 ctccgaggcc gggggcgcgg gccacgagga gggccgggcc agcccgctgc ggcgcggcta
      421 cgtgcgcgtc cagtggtacc cggagggcgt caagcagcat gtgaaggaga ccaagctgaa
      481 actggaggac cgctctgtgg tgccccggga tgtggtccgg cacatgcgct caactgacag
      541 tcagtgtggc acggtgatag acgtcaacat tgactgtgcc gtcaagctca ttggcaccaa
      601 ctgcatcatt taccctgtca acagcaagga cctccagcac atctggccct tcatgtatgg
      661 agactatatc gcctatgact gctggctggg gaaggtctat gacttgaaga accagatcat
      721 cctcaaactg tccaatgggg ccaggtgctc catgaacact gaagatggtg ccaaactcta
      781 cgatgtctgc cctcacgtca gcgactcggg tctcttcttt gatgactcct atggcttcta
      841 cccaggccaa gtacttattg gcccagccaa gatcttctcc agtgtccagt ggctctcagg
      901 ggtcaagcct gtgctcagca ccaagagcaa gttccgtgtg gtagtggagg aggtgcaggt
      961 tgtagaattg aaagtcacgt ggatcaccaa gagtttctgc cctgggggca cggacagcgt
     1021 cagcccccca ccttctgtca ttgctcagga aaacctaggc agggtgaagc gtctcggatg
     1081 ctttgaccat gctcagcggc agcttgggga acgctgtctg tatgtcttcc cagccaaggt
     1141 agagccggcc aagattgcct gggaatgtcc agaaaaaaac tgcgcccagg gggagggctc
     1201 tatggccaag aaggtgaagc gcctgttgaa gaaacaggtt gtgagaatca tgtcctgcac
     1261 cccagatgcc cagtgttcta gggaccatgc catggaggac ccagacaaga aaggggaagc
     1321 cagagccggg agcgaagtag ggtctgccag ccccgaggag caacttgacg gatcagccag
     1381 cccagtggag atgcaggatg agggatcaga ggagcttcac gagacaggag agcccctgcc
     1441 ccccttcctg ctgaaggagg gtggagatga ggggctacac tcggcagagc aggatgccga
     1501 tgatgaggca gctgatgata cagatgacac cagctcggtg acctcctctg ccagctctac
     1561 cacctcctct cagagtggca gtggcactgg ccgtaaaaag agcattccct tatccatcaa
     1621 aaacttgaag cgaaaacaca agaggaagaa gaataagatt acccgtgact tcaagcccgg
     1681 agacagggta gctgtagagg ttgtaaccac gatgacctca gctgatgtaa tgtggcagga
     1741 tggctctgtg gaatgcaata ttcgctccaa cgaccttttc cctgtgcatc acctggacaa
     1801 caacgagttc tgccctggag acttcgtggt agacaagcga gtccagagct gcccagatcc
     1861 tgctgtctat ggtgtggtcc aatctggaga ccatgttggc cgcacctgca tggtgaagtg
     1921 gttcaagctg cggccaagtg gggatgatgt ggagctcatt ggagaggagg aggacgtgag
     1981 tgtctatgac atcgctgacc accctgactt tcgcttccgc actactgata tcgtcatccg
     2041 catcggcaac actgaggatg gggctctgcc caaggaggat gagccctcag tgggccaggt
     2101 agcccgagta gacgtcagca gcaaagtgga agtggtgtgg gctgacaact cgaaaaccat
     2161 catcctgccg cagcacttat acaacattga gtccgaaatc gaggagtcag actacgactc
     2221 tgtagaaggc agcaccagtg gggcctcttc agatgagtgg gaggacgaca gcgacagctg
     2281 ggagactgac aatgggctcg tggatgatga gcaccccaag atagaagacc tggccgccat
     2341 cctgcccaca gaacagccag ctgcccccga ggaggacaag ggggtggtaa tcagtgaaga
     2401 ggcggccacg gctgccatcc agggtgctgt ggccatggct gcccccgtgg cagggctgat
     2461 ggagaaagct ggcaaggatg ggcctcccaa gagtttccgg gagctgaaag aggccatcaa
     2521 gatcctggag agcctcaaga acatgactgt ggaacaactg ctcacagggt cccccacctc
     2581 acctactgtg gagcctgaga agccaacccg agagaagaag tttttggatg atatcaagaa
     2641 gctacaggag aacctcaaga agaccctgga caatgtggcc attgctgagg aggagaagat
     2701 ggaggcagtg cccgacacag aacgcaagga ggataaacct gaggtgcagt caccagtgaa
     2761 agccgagtgg cccagtgaga cacctgtgct ctgccaacag tgtggtggca gacctggcgt
     2821 caccttcacc agcgccaagg gcgaggtgtt ctccgtgctg gagtttgcac cctcaaacca
     2881 ctctttcaag aagattgagt tccagcctcc agaagccaag aagttcttca gcacagttcg
     2941 gaaggagatg gctctgctgg ccacctcact gcccgacggc atcatggtca agacttttga
     3001 ggacagaatg gatctcttct cggcactgat caagggccct actcggactc cctacgaaga
     3061 tggcctctac ctttttgaca tccagcttcc caacatctac cctgcagtgc ccccccactt
     3121 ttgctacctc tcccagtgca gtggccgtct gaaccccaac ctgtatgaca atgggaaagt
     3181 gtgcgtcagc ctgctgggga cctggattgg aaaggggaca gagaggtgga caagcaaatc
     3241 cagtcttctc caggtactca tctccatcca aggtctgatc ctggtgaatg aaccatacta
     3301 caacgaagcc ggctttgata gtgaccgggg cctgcaggaa ggctatgaga acagccgctg
     3361 ctacaatgag atggcattga tccgtgtggt gcagtccatg acccagctgg ttcgacgacc
     3421 ccctgaggtc ttcgagcagg agatccggca gcacttcagt gtaggtggct ggcggctggt
     3481 gaaccgaatc gagtcctggc tggaaaccca cgtggtgctg gagagggcac aggcactgcc
     3541 caatggggtt cctaaggaca gcagctctct ggagccccag gctgcagccg aactctcaga
     3601 ctctggccga gaagagcctg aggatgttgg gatgacccct ggggaagcgt cccagggctc
     3661 agactcagag ggtggcgccc agggcccagc ctcggcaagc agagagcacc cagagcagac
     3721 tgagacggcc cctgatgcat cagcacctcc cagcgttaga ccaaagagga ggaggaagag
     3781 ctaccgcagc ttcctgcccg agaagagcgg ctaccctgac atcggcttcc ccctcttccc
     3841 gctctccaag ggtttcatca agagcatccg aggggtcctg acccagttcc gtgctgctct
     3901 gctggaggcg ggcatgccag agagcacaga ggacaagtag ctgaccacac ttgaaaggga
     3961 gtgtcatggt gggagaggtc agccgctgcc tgctccctcc cttcctgaga tcttgcccct
     4021 ctctctgccc cttcctgtcc ccattggagc cttctgctgc cctctcctcc ctgcggcaag
     4081 tttgatgctg aagtgcaaga agtgtcccaa gatgctgcgt tctgccctca gttatgtttc
     4141 agaggcagac acctgaggca gtgggaaccc cactgatgtc ccctgttgtc ccaggcagaa
     4201 tgtcctgaca cttggtccct agccgtccct tctcaggtca gaggtggggc gttgaggaac
     4261 ctgcagatgg caggagggtg gacgtgcctt gcatgaagtg tggctgctcc tgcaatgctc
     4321 ccagtccctc tcccttccag cttgacagta gcctccccac atcccagacc ctcctgctgc
     4381 cacaccaggt cctttattcc ccagtactcc ttctcttgga gctgtggctg ctgtggctgc
     4441 tctggccttc cagtgcatta tagcccttag gcctgttgaa cttactctct aagagaggtc
     4501 agggttaagc taaggttcag agagaccagg agaggctagc cctcacataa cacagagcta
     4561 aggacatcgt gctgcttggt aagaaatggc tgtaggtgcc ttgcccttct agtccaaggt
     4621 ggcagggaca tggcagccct ccccagacct gcctgcctgt gtgggtgggc tgttctgtca
     4681 tctgctccat tcagcctcat tttatcccta ctgaggtgtc aggagcatct gcacaaacca
     4741 ggcagcgaca agggcctctc ccatccagtg actcggcctc cagtcagcct tggcttatag
     4801 actctgcaag ggccatctga gggaggaggc tggaaggcag caggagctcc acccctcccc
     4861 cacaggccca gaggtttaca agtctctaag cttttgataa tgtgaagctc caggtagaga
     4921 ggatgccggc gagcacgttg cagctcttgt catttctggt atatgtaata tttaagggtg
     4981 aaacaaacaa atctcagaag caaagatact aactcttact agaaaagaag acgacaaaat
     5041 acaaaacaca aaaaagccaa agcatgatgc gtcttgtcgg ccttaagtgg gctccacacc
     5101 tgtgctgtgc cagggccacc agggcagcag agccttgggg agacagagcc ggaactggaa
     5161 cactcaccag ggaccagtac cttgaggaca tggggcctgg tggggcggtg cttcccagac
     5221 caccgttcac gtggagatgt gaatgcctac tgcttacaat acctgtataa agtgctgtgt
     5281 gattaaactt ttttttaacc tgcttttttg gggagggggg gttggctcat taacaaaaca
     5341 agatggcacc attaaatctg cctctgctgt tca
//
